##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935045.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1105299
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.026774655545694	33.0	31.0	34.0	30.0	34.0
2	32.19772115961382	34.0	31.0	34.0	30.0	34.0
3	32.21853091335467	34.0	31.0	34.0	30.0	34.0
4	35.80316909723071	37.0	35.0	37.0	35.0	37.0
5	35.713786043414494	37.0	35.0	37.0	33.0	37.0
6	35.64415330150484	37.0	35.0	37.0	33.0	37.0
7	35.993814343449145	37.0	35.0	37.0	35.0	37.0
8	35.796802494166734	37.0	35.0	37.0	35.0	37.0
9	37.55974808626444	39.0	37.0	39.0	35.0	39.0
10	37.261944505513895	39.0	37.0	39.0	34.0	39.0
11	37.17880682059786	39.0	37.0	39.0	34.0	39.0
12	37.06214788939464	39.0	37.0	39.0	34.0	39.0
13	36.92134345548128	39.0	37.0	39.0	33.0	39.0
14	37.95012661732255	40.0	37.0	41.0	33.0	41.0
15	38.0301990683064	40.0	37.0	41.0	33.0	41.0
16	38.09930435113033	40.0	37.0	41.0	34.0	41.0
17	38.14808029320573	40.0	37.0	41.0	34.0	41.0
18	38.156930387162205	40.0	37.0	41.0	34.0	41.0
19	38.120475093164835	40.0	37.0	41.0	34.0	41.0
20	38.07338919152193	40.0	36.0	41.0	34.0	41.0
21	37.987723683817684	40.0	36.0	41.0	34.0	41.0
22	37.9155920705619	40.0	36.0	41.0	34.0	41.0
23	37.70359423106327	39.0	36.0	41.0	33.0	41.0
24	37.44817194261462	39.0	35.0	41.0	33.0	41.0
25	37.42258701039266	39.0	35.0	41.0	33.0	41.0
26	37.61788529619587	39.0	36.0	41.0	33.0	41.0
27	37.59289929693232	39.0	36.0	41.0	33.0	41.0
28	37.6130956419937	39.0	36.0	41.0	33.0	41.0
29	37.526057654987476	39.0	36.0	41.0	33.0	41.0
30	37.35433850930834	39.0	35.0	41.0	33.0	41.0
31	37.27434115112743	39.0	35.0	41.0	33.0	41.0
32	37.06664169604786	39.0	35.0	41.0	32.0	41.0
33	37.064644951275625	39.0	35.0	41.0	32.0	41.0
34	37.048854653808604	39.0	35.0	41.0	32.0	41.0
35	36.86725582851337	39.0	35.0	41.0	31.0	41.0
36	36.8773345492939	39.0	35.0	41.0	31.0	41.0
37	36.760710902660726	39.0	35.0	40.0	31.0	41.0
38	36.60692898482673	39.0	35.0	40.0	31.0	41.0
39	36.6628966460659	39.0	35.0	40.0	31.0	41.0
40	36.69505717457448	39.0	35.0	40.0	31.0	41.0
41	36.68973734708889	39.0	35.0	41.0	31.0	41.0
42	36.69867610483679	39.0	35.0	41.0	31.0	41.0
43	36.542453218540864	39.0	35.0	40.0	31.0	41.0
44	36.36690524464421	39.0	35.0	40.0	31.0	41.0
45	36.35121084882914	39.0	35.0	40.0	31.0	41.0
46	36.240352158103825	39.0	35.0	40.0	30.0	41.0
47	36.197969961069354	38.0	35.0	40.0	30.0	41.0
48	36.18100532073222	39.0	35.0	40.0	30.0	41.0
49	36.17351142089154	39.0	35.0	40.0	30.0	41.0
50	36.09176521466137	38.0	35.0	40.0	30.0	41.0
51	35.01639918248365	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	13.0
10	23.0
11	6.0
12	15.0
13	22.0
14	25.0
15	32.0
16	66.0
17	146.0
18	241.0
19	507.0
20	987.0
21	1553.0
22	2399.0
23	3482.0
24	4870.0
25	5957.0
26	7093.0
27	8299.0
28	10324.0
29	12484.0
30	15947.0
31	20714.0
32	28040.0
33	40169.0
34	72710.0
35	103169.0
36	88453.0
37	142103.0
38	259801.0
39	275496.0
40	148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.60928219422979	23.31332969630842	26.464151329187846	10.613236780273935
2	28.959765638076213	25.57688010212621	26.18893168273924	19.274422577058335
3	29.652519363538737	25.04833533731596	28.1805194793445	17.118625819800794
4	28.594525101352662	25.769950031620404	27.542502074099406	18.093022792927524
5	22.96925990161938	28.562316622018113	27.542683020612525	20.925740455749985
6	24.003097804304538	32.057208049586585	26.863771703403334	17.075922442705547
7	76.08818971156222	6.3165713530908825	10.894880027938141	6.700358907408764
8	75.38412682902998	4.332402363523355	10.811101792365687	9.472369015080988
9	70.20806134810582	6.243107068766008	14.190549344566492	9.358282238561692
10	36.83781492609692	22.94817963284143	22.816179151523706	17.397826289537942
11	26.193093452540893	25.349249388626973	26.09502044243232	22.362636716399816
12	23.88512067775326	22.600038541607294	32.75095698087124	20.763883799768205
13	21.419453016785504	27.16097635119547	33.04047140185597	18.37909923016306
14	16.40415851276442	31.801892519580676	29.524680652022667	22.26926831563224
15	15.086053637974883	26.880780675636185	37.810221487579376	20.222944198809554
16	15.66273017527384	27.801255587854506	32.334689527449136	24.20132470942252
17	16.090035365995988	27.390687949595538	26.89046131408786	29.62881537032061
18	17.830288455883885	26.77981252131776	33.05277576474782	22.337123258050536
19	18.65667118128217	28.029519614149656	29.46469688292489	23.849112321643283
20	20.573347121457633	29.09737546130052	28.530198615940122	21.79907880130173
21	19.86512246912374	29.302025967634098	30.335411504036465	20.497440059205697
22	17.12767314545657	27.70182547889756	26.517711497070025	28.65278987857584
23	16.564024757101926	27.707344347547586	28.012420168660245	27.716210726690242
24	20.763702853255094	25.18069771165992	29.937872014721805	24.117727420363178
25	17.22791751372253	27.862325036030978	27.044356323492558	27.865401126753937
26	18.967446817557963	29.232994872880553	28.507761248313805	23.291797061247678
27	21.227016400087216	30.43710344440735	26.83672019969257	21.49915995581286
28	18.634957599708315	26.9389549796028	28.919233619138353	25.506853801550534
29	21.331874904437623	23.919048148962407	30.559604233786512	24.189472712813455
30	22.746967110257042	28.416383259190496	27.6015811106316	21.235068519920855
31	23.728873363678062	29.32545854108255	22.555344752867775	24.390323342371612
32	21.730771492600645	30.219877155412245	25.272618540322572	22.776732811664534
33	20.69494317827122	27.241226129762175	26.33902681536851	25.724803876598095
34	20.054392521842505	23.989345869307762	27.884400510631057	28.07186109821867
35	19.4724685356632	26.330522329252087	27.07964089355007	27.117368241534646
36	22.67865980155596	26.06344527589367	26.574981068471065	24.682913854079302
37	20.74696530079191	26.33251274089635	29.271265060404474	23.649256897907264
38	18.13970699331131	28.104250524066337	28.879606332766066	24.876436149856282
39	20.035935977504728	21.425695671487986	32.66989294299552	25.868475408011772
40	22.08931700833892	22.219146131499258	30.358844077484914	25.3326927826769
41	18.933790766118488	26.66834946923864	24.171920901041258	30.225938863601616
42	22.62926140347544	24.22638580148901	26.16622289534325	26.9781298996923
43	24.333325190740243	22.90312395107568	28.345271279536128	24.41827957864795
44	21.777636639497548	22.188385224269634	28.40326463698963	27.630713499243193
45	21.80830707347062	20.89669854039495	28.604658106087133	28.6903362800473
46	24.15789754627481	22.409863756322952	29.92149635528486	23.510742342117382
47	17.899138604124314	23.626819530280947	32.00572876660524	26.468313098989505
48	18.838522426963202	21.344360213842588	30.93868717876339	28.878430180430815
49	21.687525275966053	17.733029705084324	34.52577085476419	26.053674164185438
50	20.35928739644205	18.267726651340496	32.99812991778695	28.3748560344305
51	18.374213674308944	18.94329045805705	27.936060740125523	34.746435127508484
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1964.0
1	1847.5
2	1731.0
3	1925.5
4	2120.0
5	1756.5
6	1393.0
7	1543.0
8	1693.0
9	1801.5
10	1910.0
11	2131.5
12	2353.0
13	2487.5
14	2622.0
15	2614.5
16	2607.0
17	2649.5
18	2692.0
19	2930.5
20	3169.0
21	3271.0
22	3373.0
23	3262.0
24	3151.0
25	3269.5
26	4599.0
27	5810.0
28	5802.0
29	5794.0
30	7330.5
31	8867.0
32	10371.0
33	11875.0
34	14021.5
35	16168.0
36	19294.0
37	22420.0
38	25111.0
39	27802.0
40	33312.0
41	38822.0
42	50198.5
43	61575.0
44	77984.5
45	94394.0
46	116938.5
47	139483.0
48	164866.0
49	190249.0
50	180283.5
51	170318.0
52	135990.5
53	101663.0
54	82888.5
55	64114.0
56	50367.0
57	36620.0
58	30655.0
59	24690.0
60	22029.5
61	19369.0
62	15663.5
63	11958.0
64	9827.5
65	7697.0
66	6073.0
67	4449.0
68	3921.5
69	3394.0
70	2330.0
71	1266.0
72	1036.0
73	806.0
74	572.5
75	446.0
76	553.0
77	433.0
78	313.0
79	242.5
80	172.0
81	114.5
82	57.0
83	37.0
84	17.0
85	40.5
86	64.0
87	37.0
88	10.0
89	6.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1105299.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.65340669013367
#Duplication Level	Percentage of deduplicated	Percentage of total
1	62.44112182367492	13.52063005036214
2	12.390359946937334	5.365870059363543
3	6.335246994909951	4.115390389896971
4	3.999394027371651	3.464020215550798
5	2.655977851404549	2.875548428822506
6	1.9013933801612741	2.470298648313601
7	1.4234054176875404	2.157510347588953
8	1.108445823409134	1.9201302566606453
9	0.8507215276866286	1.6578917297145341
>10	5.3580350959394005	22.691538163581388
>50	1.0177434800896727	16.025693966268204
>100	0.4924156792016149	15.797185983834765
>500	0.017299951025934984	2.4174676773508397
>1k	0.006751200400364872	2.4660746845828077
>5k	0.0012658500750684135	1.989506733977646
>10k+	4.219500250228045E-4	1.0652426641306716
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	11659	1.0548276982065488	TruSeq Adapter, Index 19 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	9398	0.850267665129526	TruSeq Adapter, Index 19 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT	7303	0.6607261926410862	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	5074	0.45906130377391097	TruSeq Adapter, Index 19 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	3807	0.3444316877152698	TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	2281	0.20636949820817713	No Hit
ACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	2047	0.18519875617366885	No Hit
AGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	2005	0.18139887939824428	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	1840	0.1664707920662192	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1711	0.1547997419702723	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCC	1693	0.1531712233522332	No Hit
AAAACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC	1605	0.14520957677515314	No Hit
GGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	1588	0.14367153141367178	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC	1475	0.13344805342264854	No Hit
GGCCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT	1282	0.11598671490700707	No Hit
AGCCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT	1212	0.1096535869479661	No Hit
TGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTG	1193	0.10793459507336929	No Hit
TTGTCTGGGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAAAAGAAAGAAA	1173	0.10612512994221472	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6925727789494065	0.0
2	0.0	0.0	0.0	3.566365300249073	0.0
3	0.0	0.0	0.0	4.993399975934114	0.0
4	0.0	0.0	0.0	6.670321786231598	0.0
5	0.0	0.0	0.0	10.684982072724214	0.0
6	0.0	0.0	0.0	12.821417553078398	0.0
7	0.0	0.0	0.0	15.01331313970247	0.0
8	0.0	0.0	0.0	18.260579264072437	0.0
9	0.0	0.0	0.0	19.363629208024253	0.0
10	0.0	0.0	0.0	21.818349604948526	0.0
11	0.0	0.0	0.0	25.80460128888201	0.0
12	0.0	0.0	0.0	28.545850489324607	0.0
13	0.0	0.0	0.0	29.600406767761484	0.0
14	0.0	0.0	0.0	29.97722788132442	0.0
15	0.0	0.0	0.0	30.67369101030581	0.0
16	0.0	0.0	0.0	32.35314607178691	0.0
17	0.0	0.0	0.0	34.40797467472603	0.0
18	0.0	0.0	0.0	36.83772445284036	0.0
19	0.0	0.0	0.0	38.1001882748469	0.0
20	0.0	0.0	0.0	39.182519843047	0.0
21	0.0	0.0	0.0	40.63895832711329	0.0
22	0.0	0.0	0.0	41.94394457970196	0.0
23	0.0	0.0	0.0	43.282044044190755	0.0
24	0.0	0.0	0.0	44.34365723663914	0.0
25	0.0	0.0	0.0	45.25942753951646	0.0
26	0.0	0.0	0.0	46.07323448225322	0.0
27	0.0	0.0	0.0	46.78978267419042	0.0
28	0.0	0.0	0.0	47.53030627911542	0.0
29	0.0	0.0	0.0	48.283948506241295	0.0
30	0.0	0.0	0.0	49.1941999404686	0.0
31	0.0	0.0	0.0	49.99787387847089	0.0
32	0.0	0.0	0.0	50.78707209542395	0.0
33	0.0	0.0	0.0	51.509048682754624	0.0
34	0.0	0.0	0.0	52.174298538223596	0.0
35	0.0	0.0	0.0	52.94829724807496	0.0
36	0.0	0.0	0.0	53.61390899657016	0.0
37	0.0	0.0	0.0	54.27128767871861	0.0
38	9.047325655772782E-5	0.0	0.0	54.914190639817825	0.0
39	9.047325655772782E-5	0.0	0.0	55.52759931927922	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCGAT	20	7.033167E-4	45.000004	36
AGTCCGC	20	7.033167E-4	45.000004	39
AGTCCAT	20	7.033167E-4	45.000004	25
TCGCACG	35	1.2118653E-7	45.000004	1
GCGACCG	20	7.033167E-4	45.000004	10
TCCGCAA	20	7.033167E-4	45.000004	39
CGTATAC	20	7.033167E-4	45.000004	24
ATAGGCG	20	7.033167E-4	45.000004	1
CTATCCA	20	7.033167E-4	45.000004	31
GAGTCGA	20	7.033167E-4	45.000004	20
GCGAAAG	55	1.8189894E-12	45.000004	1
CGGTTTA	20	7.033167E-4	45.000004	19
TTTCGCG	20	7.033167E-4	45.000004	1
TTTCGCA	20	7.033167E-4	45.000004	15
CGTTGAT	20	7.033167E-4	45.000004	25
ATTACGC	40	6.8139343E-9	45.000004	12
TAGCGAT	20	7.033167E-4	45.000004	29
GATCCGT	20	7.033167E-4	45.000004	7
TTGTCCG	20	7.033167E-4	45.000004	1
TTGTCAA	20	7.033167E-4	45.000004	30
>>END_MODULE