FastQCFastQC Report
Sat 14 Jan 2017
SRR2935044.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935044.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences673385
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC64450.9571047766136758No Hit
CCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC58920.8749823652145503No Hit
CTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGCT37500.5568879615673055TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC25470.3782383034965139No Hit
ACTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC20980.3115602515648552TruSeq Adapter, Index 13 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14340.21295395650333762No Hit
GCCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG12650.18785687236870438No Hit
AGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG11440.16988795414213265No Hit
CGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG11260.1672148919266096No Hit
AAAACTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTC11200.1663238711881019No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGC10660.1583046845415327No Hit
GAATCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTC10660.1583046845415327No Hit
ACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG10260.15236454628481477No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCG9750.14479087000749943No Hit
GGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG9240.13721719373018407No Hit
AGACATAGACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCC9080.1348411384274969No Hit
AGGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCT8510.12637644141167387No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCC8450.12548542067316618No Hit
CGTTCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTC8230.12221834463197132No Hit
GGCCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCT7950.11806024785226876No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTCAC453.8380676E-1045.00000415
TTCTAAC302.1641572E-645.00000431
AGCGTGT302.1641572E-645.00000443
TTTACGG302.1641572E-645.0000042
CACGGCC302.1641572E-645.00000441
GGGTCTA302.1641572E-645.0000047
CCGGCTA302.1641572E-645.00000419
TAGAACG302.1641572E-645.0000041
TAGCGCA302.1641572E-645.0000041
CGCGCAA302.1641572E-645.00000418
TGAGTCG302.1641572E-645.0000041
TTATCGT302.1641572E-645.00000427
GTAATTT302.1641572E-645.0000049
AATATCG302.1641572E-645.00000423
GACCACG453.8380676E-1045.00000410
ATATCGT302.1641572E-645.00000424
CGGATTC302.1641572E-645.0000045
ACTACTA302.1641572E-645.00000419
ACCACGA453.8380676E-1045.00000411
GTTCACC302.1641572E-645.00000414
Produced by FastQC (version 0.11.5)