##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935044.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	673385
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09274783370583	33.0	31.0	34.0	30.0	34.0
2	32.27555707359089	34.0	31.0	34.0	30.0	34.0
3	32.28042353185771	34.0	31.0	34.0	30.0	34.0
4	35.847227069210035	37.0	35.0	37.0	35.0	37.0
5	35.75603703676203	37.0	35.0	37.0	35.0	37.0
6	35.68282483274798	37.0	35.0	37.0	33.0	37.0
7	35.99941192631258	37.0	35.0	37.0	35.0	37.0
8	35.70621115706468	37.0	35.0	37.0	35.0	37.0
9	37.4309897012853	39.0	37.0	39.0	35.0	39.0
10	37.289171870475286	39.0	37.0	39.0	34.0	39.0
11	37.1845363350832	39.0	37.0	39.0	34.0	39.0
12	36.9224366447129	39.0	35.0	39.0	33.0	39.0
13	36.67351515106514	39.0	35.0	39.0	33.0	39.0
14	37.642017567958895	39.0	36.0	41.0	33.0	41.0
15	37.79270402518619	40.0	36.0	41.0	33.0	41.0
16	37.91920075439756	40.0	36.0	41.0	33.0	41.0
17	37.99673143892424	40.0	36.0	41.0	34.0	41.0
18	38.00762862255619	39.0	36.0	41.0	34.0	41.0
19	37.953216956124656	40.0	36.0	41.0	34.0	41.0
20	37.88912731943836	40.0	36.0	41.0	34.0	41.0
21	37.83441567602486	39.0	35.0	41.0	34.0	41.0
22	37.726951149788015	39.0	35.0	41.0	33.0	41.0
23	37.50289061977917	39.0	35.0	41.0	33.0	41.0
24	37.275530342968736	39.0	35.0	41.0	33.0	41.0
25	37.23817281347223	39.0	35.0	41.0	33.0	41.0
26	37.396232467310675	39.0	35.0	41.0	33.0	41.0
27	37.47893701225896	39.0	35.0	41.0	33.0	41.0
28	37.4733013060879	39.0	35.0	41.0	33.0	41.0
29	37.44933433325661	39.0	35.0	41.0	33.0	41.0
30	37.428413166316446	39.0	35.0	41.0	33.0	41.0
31	37.33321057047603	39.0	35.0	41.0	33.0	41.0
32	37.16308649583819	39.0	35.0	41.0	33.0	41.0
33	37.143353356549376	39.0	35.0	41.0	33.0	41.0
34	37.03324992389198	39.0	35.0	41.0	33.0	41.0
35	36.93229875925362	39.0	35.0	41.0	32.0	41.0
36	36.92530276142177	39.0	35.0	41.0	32.0	41.0
37	36.81292277077749	39.0	35.0	41.0	32.0	41.0
38	36.70467117622162	39.0	35.0	41.0	31.0	41.0
39	36.67738960624308	39.0	35.0	41.0	31.0	41.0
40	36.62358680398286	39.0	35.0	41.0	31.0	41.0
41	36.608807740000145	39.0	35.0	41.0	31.0	41.0
42	36.596519078981565	39.0	35.0	41.0	31.0	41.0
43	36.44951105236974	39.0	35.0	40.0	31.0	41.0
44	36.26785716937562	38.0	35.0	40.0	31.0	41.0
45	36.28818580752467	39.0	35.0	40.0	31.0	41.0
46	36.2082419418312	38.0	35.0	40.0	31.0	41.0
47	36.14290933121468	38.0	35.0	40.0	31.0	41.0
48	36.12284799928718	38.0	35.0	40.0	31.0	41.0
49	36.114904549403384	38.0	35.0	40.0	31.0	41.0
50	35.996620061331924	38.0	35.0	40.0	30.0	41.0
51	34.961635617068985	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	2.0
10	11.0
11	12.0
12	10.0
13	5.0
14	10.0
15	25.0
16	52.0
17	105.0
18	180.0
19	384.0
20	640.0
21	1034.0
22	1628.0
23	2334.0
24	3268.0
25	3936.0
26	4607.0
27	5284.0
28	6264.0
29	7446.0
30	9683.0
31	12722.0
32	16901.0
33	25070.0
34	50111.0
35	74319.0
36	47953.0
37	74620.0
38	141502.0
39	183121.0
40	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.027643918412195	22.89626291051924	25.1530699377028	10.923023233365758
2	30.996383940836225	24.53128596568085	24.895119433904824	19.5772106595781
3	28.87263601060315	25.873163197873428	27.955924174135156	17.298276617388268
4	28.71982595394908	25.88192490180209	26.738492838420814	18.65975630582802
5	24.695530788479104	30.19312874507154	25.630954060455757	19.4803864059936
6	24.537968621219658	33.055384364071074	26.03874455177944	16.367902462929827
7	75.12433451888593	7.2166739680866065	11.120235823488791	6.538755689538673
8	73.0886491383087	6.139132888317976	11.414718177565582	9.357499795807747
9	67.61302969326611	8.058391559063537	14.86935408421631	9.459224663454043
10	40.30190752689769	24.670136697431634	20.257802000341556	14.77015377532912
11	31.6217319958122	24.130920647178066	24.16938304239031	20.07796431461942
12	30.38425269348144	19.76328549046979	30.83807925629469	19.01438255975408
13	23.024272889951515	29.40071430162537	30.244807947904988	17.33020486051813
14	15.340407047974042	34.68506129480164	28.75591229385864	21.218619363365683
15	13.218738166130816	27.325824008553802	40.07618227314241	19.379255552172978
16	13.03399986634689	26.5340035789333	37.97916496506456	22.45283158965525
17	14.23613534605018	25.11683509433682	29.6301521417911	31.0168774178219
18	16.938601245944	27.14554081246241	33.6297957334957	22.286062208097892
19	19.843922867304737	26.919221544881456	28.490982127609023	24.745873460204788
20	22.336107872910745	27.899344357239915	28.130712742339075	21.633835027510266
21	19.460932453202847	28.900257653496887	30.645618776777027	20.99319111652324
22	16.404285809752224	28.826005925287912	25.817028891347448	28.952679373612423
23	16.030651113404666	30.05115944073598	27.52630367471803	26.39188577114132
24	20.814689961908865	24.64726716514327	30.00779643146194	24.530246441485925
25	16.509723263808965	28.077400001485035	28.00819739079427	27.40467934391173
26	15.468120020493478	32.10867482940665	28.046808289462938	24.37639686063693
27	19.32906138390371	30.323811786719336	26.950407270729226	23.396719558647728
28	17.228628496328252	28.65433592966876	29.41942573713403	24.697609836868953
29	17.423019520779345	29.217015526036366	28.922236165046744	24.437728788137544
30	19.676856478834544	27.85033821662199	27.20969430563496	25.263110998908502
31	21.860451302004055	29.68079182042962	22.195623603139364	26.263133274426963
32	20.817214520667967	31.70222086919073	24.60598320425908	22.87458140588222
33	23.40325371073012	26.148340102615887	25.5121512953214	24.936254891332595
34	20.79538451257453	25.057136704856802	27.70569585007091	26.441782932497755
35	16.627635008204816	27.651046578109106	27.621494390281935	28.099824023404146
36	24.141612896040154	24.61979402570595	27.54353007566251	23.695063002591386
37	21.591214535518315	25.905982461741793	28.728736161334155	23.774066841405734
38	20.9192363952271	28.148087646739977	26.745026990503202	24.187648967529718
39	22.17126903628682	24.758793260913148	29.74821238964337	23.321725313156662
40	22.27670649034356	21.272080607676145	31.8897807346466	24.561432167333695
41	18.496551007224692	24.36050699080021	27.588081112587897	29.554860889387204
42	24.429561098034558	23.108177342827656	28.014286032507407	24.447975526630383
43	26.254965584323976	22.39743980041135	27.482495155074734	23.86509946018994
44	21.47731238444575	24.470251045093075	27.369781031653513	26.682655538807666
45	20.580648514594177	24.308976291423182	26.13898438486156	28.971390809121083
46	22.58886075573409	23.64561135160421	29.63980486645827	24.125723026203435
47	18.459276639663788	22.90725216629417	32.51052518247362	26.12294601156842
48	20.3921976283998	20.286017657060967	31.624256554571307	27.69752815996792
49	22.424912939848678	17.65067531946806	34.29390319059676	25.630508550086507
50	20.057916348002998	20.122069841175552	32.32742042071029	27.492593390111153
51	18.228799275303132	20.211468921939158	27.237464451985122	34.32226735077259
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1651.0
1	1551.5
2	1452.0
3	1600.5
4	1749.0
5	1470.5
6	1192.0
7	1268.0
8	1344.0
9	1472.0
10	1600.0
11	1665.5
12	1731.0
13	1868.5
14	2006.0
15	2068.5
16	2131.0
17	2182.0
18	2233.0
19	2036.0
20	1839.0
21	2079.0
22	2319.0
23	2198.5
24	2078.0
25	2560.5
26	3328.5
27	3614.0
28	4147.5
29	4681.0
30	5238.5
31	5796.0
32	7652.0
33	9508.0
34	11121.5
35	12735.0
36	12980.0
37	13225.0
38	16830.0
39	20435.0
40	22489.0
41	24543.0
42	33078.0
43	41613.0
44	49883.0
45	58153.0
46	69158.5
47	80164.0
48	91509.0
49	102854.0
50	98688.5
51	94523.0
52	75327.0
53	56131.0
54	45356.0
55	34581.0
56	28502.0
57	22423.0
58	19517.0
59	16611.0
60	15546.5
61	14482.0
62	12963.5
63	11445.0
64	9606.5
65	7768.0
66	5957.5
67	4147.0
68	3528.5
69	2910.0
70	2545.0
71	2180.0
72	1564.5
73	949.0
74	859.5
75	571.5
76	373.0
77	243.5
78	114.0
79	97.5
80	81.0
81	75.5
82	70.0
83	47.0
84	24.0
85	66.5
86	109.0
87	56.5
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	673385.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.389901510176283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.37563117699072	14.41364041708256
2	12.373020738986332	5.540614314585451
3	6.146447635312702	4.12855071576322
4	3.716087149233508	3.3281130109828
5	2.4871604046750004	2.7843638250341725
6	1.7300770697225583	2.324175311766148
7	1.2673711294460424	1.9863420335596436
8	0.9786441071384504	1.752939613787545
9	0.7089009954157223	1.4285001121041554
>10	4.5323914586614915	19.85746236667543
>50	1.065721331475617	17.3032589240114
>100	0.5984641145346745	18.91000616872015
>500	0.01138019009741551	1.7486865768672613
>1k	0.0073636524159747425	2.644238329398494
>5k	0.0013388458938135893	1.8491082796615586
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	6445	0.9571047766136758	No Hit
CCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	5892	0.8749823652145503	No Hit
CTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGCT	3750	0.5568879615673055	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	2547	0.3782383034965139	No Hit
ACTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	2098	0.3115602515648552	TruSeq Adapter, Index 13 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1434	0.21295395650333762	No Hit
GCCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG	1265	0.18785687236870438	No Hit
AGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG	1144	0.16988795414213265	No Hit
CGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG	1126	0.1672148919266096	No Hit
AAAACTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTC	1120	0.1663238711881019	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGC	1066	0.1583046845415327	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTC	1066	0.1583046845415327	No Hit
ACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG	1026	0.15236454628481477	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCG	975	0.14479087000749943	No Hit
GGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG	924	0.13721719373018407	No Hit
AGACATAGACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCC	908	0.1348411384274969	No Hit
AGGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCT	851	0.12637644141167387	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCC	845	0.12548542067316618	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTC	823	0.12221834463197132	No Hit
GGCCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCT	795	0.11806024785226876	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4850345641794814E-4	0.0	0.0	0.5861431424816412	0.0
2	1.4850345641794814E-4	0.0	0.0	3.235147798065	0.0
3	1.4850345641794814E-4	0.0	0.0	4.485398397647705	0.0
4	1.4850345641794814E-4	0.0	0.0	5.930782538963594	0.0
5	1.4850345641794814E-4	0.0	0.0	9.646636025453493	0.0
6	1.4850345641794814E-4	0.0	0.0	11.518225086688894	0.0
7	1.4850345641794814E-4	0.0	0.0	13.429613074244303	0.0
8	1.4850345641794814E-4	0.0	0.0	16.32483646056862	0.0
9	1.4850345641794814E-4	0.0	0.0	17.358420517237537	0.0
10	1.4850345641794814E-4	0.0	0.0	19.62948387623722	0.0
11	1.4850345641794814E-4	0.0	0.0	23.128373812900495	0.0
12	1.4850345641794814E-4	0.0	0.0	25.743074170051308	0.0
13	1.4850345641794814E-4	0.0	0.0	26.75126413567276	0.0
14	1.4850345641794814E-4	0.0	0.0	27.13024495645136	0.0
15	1.4850345641794814E-4	0.0	0.0	27.884345508141703	0.0
16	1.4850345641794814E-4	0.0	0.0	29.48476725795793	0.0
17	1.4850345641794814E-4	0.0	0.0	31.180231219881644	0.0
18	8.910207385076888E-4	0.0	0.0	33.365756587984585	0.0
19	8.910207385076888E-4	0.0	0.0	34.47002828990845	0.0
20	8.910207385076888E-4	0.0	0.0	35.45787328200064	0.0
21	8.910207385076888E-4	0.0	0.0	36.762327643175894	0.0
22	8.910207385076888E-4	0.0	0.0	37.99312428996785	0.0
23	8.910207385076888E-4	0.0	0.0	39.2213963780007	0.0
24	8.910207385076888E-4	0.0	0.0	40.05702532726449	0.0
25	8.910207385076888E-4	0.0	0.0	40.79092940888199	0.0
26	8.910207385076888E-4	0.0	0.0	41.524090973217405	0.0
27	8.910207385076888E-4	0.0	0.0	42.17839720219488	0.0
28	8.910207385076888E-4	0.0	0.0	42.80805185740698	0.0
29	8.910207385076888E-4	0.0	0.0	43.41246092502803	0.0
30	8.910207385076888E-4	0.0	0.0	44.157057255507624	0.0
31	8.910207385076888E-4	0.0	0.0	44.89853501340244	0.0
32	8.910207385076888E-4	0.0	0.0	45.585512002791866	0.0
33	8.910207385076888E-4	0.0	0.0	46.199276788167246	0.0
34	8.910207385076888E-4	0.0	0.0	46.74888808037007	0.0
35	8.910207385076888E-4	0.0	0.0	47.45279446379114	0.0
36	8.910207385076888E-4	0.0	0.0	48.01621657744084	0.0
37	8.910207385076888E-4	0.0	0.0	48.6082998581792	0.0
38	8.910207385076888E-4	0.0	0.0	49.13474461118083	0.0
39	8.910207385076888E-4	0.0	0.0	49.65539772938215	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGTCAC	45	3.8380676E-10	45.000004	15
TTCTAAC	30	2.1641572E-6	45.000004	31
AGCGTGT	30	2.1641572E-6	45.000004	43
TTTACGG	30	2.1641572E-6	45.000004	2
CACGGCC	30	2.1641572E-6	45.000004	41
GGGTCTA	30	2.1641572E-6	45.000004	7
CCGGCTA	30	2.1641572E-6	45.000004	19
TAGAACG	30	2.1641572E-6	45.000004	1
TAGCGCA	30	2.1641572E-6	45.000004	1
CGCGCAA	30	2.1641572E-6	45.000004	18
TGAGTCG	30	2.1641572E-6	45.000004	1
TTATCGT	30	2.1641572E-6	45.000004	27
GTAATTT	30	2.1641572E-6	45.000004	9
AATATCG	30	2.1641572E-6	45.000004	23
GACCACG	45	3.8380676E-10	45.000004	10
ATATCGT	30	2.1641572E-6	45.000004	24
CGGATTC	30	2.1641572E-6	45.000004	5
ACTACTA	30	2.1641572E-6	45.000004	19
ACCACGA	45	3.8380676E-10	45.000004	11
GTTCACC	30	2.1641572E-6	45.000004	14
>>END_MODULE