##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935043.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1377403
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37602212279195	33.0	31.0	34.0	31.0	34.0
2	32.543808892531814	34.0	31.0	34.0	31.0	34.0
3	32.54417697652757	34.0	31.0	34.0	31.0	34.0
4	36.04545002443003	37.0	35.0	37.0	35.0	37.0
5	35.971733036736524	37.0	35.0	37.0	35.0	37.0
6	35.916310622236196	37.0	35.0	37.0	35.0	37.0
7	36.159740468112815	37.0	35.0	37.0	35.0	37.0
8	35.93941569751191	37.0	36.0	37.0	35.0	37.0
9	37.65705026052651	39.0	38.0	39.0	35.0	39.0
10	37.489032621534875	39.0	37.0	39.0	35.0	39.0
11	37.40922373481109	39.0	37.0	39.0	35.0	39.0
12	37.15369721134628	39.0	37.0	39.0	34.0	39.0
13	36.99825613854478	39.0	37.0	39.0	33.0	39.0
14	38.08747621429603	40.0	37.0	41.0	33.0	41.0
15	38.19261755637239	40.0	37.0	41.0	33.0	41.0
16	38.29206484957562	40.0	37.0	41.0	34.0	41.0
17	38.33122985792829	40.0	37.0	41.0	34.0	41.0
18	38.31612825004737	40.0	37.0	41.0	34.0	41.0
19	38.28443237019231	40.0	37.0	41.0	34.0	41.0
20	38.267688541407274	40.0	37.0	41.0	34.0	41.0
21	38.1822502201607	40.0	36.0	41.0	34.0	41.0
22	38.13201437778196	40.0	36.0	41.0	34.0	41.0
23	37.94727832014305	40.0	36.0	41.0	34.0	41.0
24	37.74319207958746	40.0	35.0	41.0	33.0	41.0
25	37.693653926991594	40.0	35.0	41.0	33.0	41.0
26	37.84769962022734	40.0	36.0	41.0	34.0	41.0
27	37.8612439496647	40.0	36.0	41.0	34.0	41.0
28	37.845196358654654	40.0	36.0	41.0	34.0	41.0
29	37.87338854351268	40.0	36.0	41.0	34.0	41.0
30	37.78726705256196	40.0	36.0	41.0	34.0	41.0
31	37.6994372743489	40.0	35.0	41.0	33.0	41.0
32	37.6131705826109	40.0	35.0	41.0	33.0	41.0
33	37.51493644198539	40.0	35.0	41.0	33.0	41.0
34	37.40977114177913	40.0	35.0	41.0	33.0	41.0
35	37.32641862984181	40.0	35.0	41.0	33.0	41.0
36	37.257955732635985	39.0	35.0	41.0	33.0	41.0
37	37.23177748269751	39.0	35.0	41.0	33.0	41.0
38	37.1484670789885	39.0	35.0	41.0	33.0	41.0
39	37.11020884955238	39.0	35.0	41.0	33.0	41.0
40	37.02206761565061	39.0	35.0	41.0	32.0	41.0
41	36.942740795540594	39.0	35.0	41.0	32.0	41.0
42	36.91651245133051	39.0	35.0	41.0	32.0	41.0
43	36.865538988952395	39.0	35.0	41.0	32.0	41.0
44	36.76125723553673	39.0	35.0	41.0	32.0	41.0
45	36.73191433443952	39.0	35.0	41.0	32.0	41.0
46	36.61408462156682	39.0	35.0	41.0	32.0	41.0
47	36.53825931844202	38.0	35.0	41.0	31.0	41.0
48	36.47225830058451	38.0	35.0	41.0	31.0	41.0
49	36.43194620601233	38.0	35.0	40.0	31.0	41.0
50	36.33706547756902	38.0	35.0	40.0	31.0	41.0
51	35.330569194346175	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	11.0
10	30.0
11	19.0
12	19.0
13	21.0
14	36.0
15	37.0
16	110.0
17	192.0
18	288.0
19	613.0
20	946.0
21	1508.0
22	2386.0
23	3265.0
24	4782.0
25	6302.0
26	7681.0
27	9169.0
28	11113.0
29	13513.0
30	17037.0
31	22387.0
32	30396.0
33	45024.0
34	93520.0
35	130872.0
36	95863.0
37	150453.0
38	277134.0
39	452227.0
40	438.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.46258865415568	21.133175984080186	24.723120248757986	10.681115113006143
2	32.05684901223535	23.863458987674633	25.38639744504695	18.693294555043078
3	32.28423344511374	24.809950319550634	25.868681859993043	17.037134375342582
4	28.514385404997668	26.403528959934018	25.45493221664248	19.627153418425834
5	25.25404692744244	30.124153933162624	24.397289682104656	20.224509457290278
6	26.303703418679937	33.95099328228557	23.105147876111783	16.640155422922703
7	81.65431612970205	6.277320435631402	7.466805285018255	4.601558149648287
8	82.82122225666707	4.458825775753357	7.231362208445895	5.488589759133674
9	77.04034331274144	7.250238310792121	9.144019578874156	6.565398797592281
10	42.321818668900825	27.701478797418037	15.885909933403658	14.09079260027748
11	34.06773471525762	24.38255180219587	21.91479182200126	19.634921660545242
12	30.824457330207643	23.40549570459771	26.740176985239618	19.02986997995503
13	25.336375773829445	27.108914384533794	28.359456164971323	19.195253676665438
14	19.913707172120287	30.050900135980534	28.826349296465885	21.20904339543329
15	18.004389419799434	25.350315049408195	36.04166681791749	20.60362871287488
16	21.408258875579623	25.857791800947144	32.217223281784634	20.5167260416886
17	22.649362604844043	24.01817042652005	27.551268582978256	25.781198385657646
18	24.698145713346058	24.188127948029734	29.574133350951026	21.539592987673178
19	25.503429279593554	26.47881556813801	25.584741720469605	22.433013431798827
20	26.994060561796367	27.34646287252169	25.94215345835605	19.71732310732589
21	24.590697130759843	27.244314118671152	27.693928356479546	20.471060394089456
22	22.91261163218027	25.168160661767107	26.941207475226932	24.978020230825692
23	21.941508766860533	27.138825746713195	27.857569643742607	23.062095842683657
24	22.752600364599175	25.11102415197295	28.78525747366602	23.351118009761848
25	21.544457214047014	27.20750571909601	27.59824103766291	23.64979602919407
26	21.08395291719272	28.251499379629635	25.94026584812143	24.72428185505622
27	21.671072300554016	28.72441834379626	27.00444241808679	22.600066937562936
28	19.497924717747818	27.269506455264004	28.58364618052959	24.64892264645859
29	20.59070584280708	26.006985609875976	27.89684645670149	25.505462090615456
30	22.488189730964724	27.298474012326096	26.968940825597155	23.24439543111203
31	24.946148658018025	27.37114700635907	24.72515305977989	22.95755127584302
32	25.236695433362637	26.4210256548011	25.19908842945747	23.143190482378795
33	23.95805730058668	28.664668219831086	24.087939404807454	23.289335074774776
34	21.70766289894824	26.534935672421216	27.565207858557013	24.192193570073535
35	20.498575943278766	27.547783764083565	27.384868480756904	24.56877181188076
36	24.076105540644242	30.157913116204917	24.54546708552254	21.220514257628306
37	23.24751724803852	28.731750983553834	26.04938423976135	21.9713475286463
38	21.853444489376024	29.308706311805622	25.121769010231574	23.716080188586783
39	22.75020455160908	27.411512825222538	25.298333167562436	24.539949455605946
40	25.123729220859836	24.70743856373189	28.120383068716997	22.048449146691272
41	20.976431734212863	25.648412265691306	27.126991882549987	26.248164117545848
42	22.815036703129003	25.563832807101477	27.127863087273656	24.493267402495857
43	23.02492444114032	26.03392035591617	26.41855724141736	24.522597961526145
44	22.418638553858237	25.577699482286594	26.320546710004262	25.683115253850907
45	22.288320847275635	24.945422654081632	25.240325453044605	27.525931045598128
46	22.74882514412993	26.5758822944338	26.420807853620182	24.254484707816086
47	20.203890945496706	27.452822449203317	29.173161376880984	23.17012522841899
48	21.29601866701321	25.89118798202124	28.220789413120194	24.592003937845348
49	22.75666598664298	24.460815026539073	28.934088280626657	23.84843070619129
50	20.332538843025606	26.467272105549355	28.0417568424056	25.158432209019438
51	20.402816024068482	25.2846116931646	25.696546326674184	28.616025956092734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	782.0
1	1198.5
2	1615.0
3	2087.0
4	2559.0
5	2054.5
6	1550.0
7	1578.0
8	1606.0
9	1701.5
10	1797.0
11	1821.5
12	1846.0
13	2212.5
14	2579.0
15	2371.0
16	2163.0
17	2344.5
18	2526.0
19	2750.5
20	2975.0
21	3289.0
22	3603.0
23	3762.0
24	3921.0
25	5006.0
26	8399.5
27	10708.0
28	11675.5
29	12643.0
30	14777.0
31	16911.0
32	20133.5
33	23356.0
34	30378.5
35	37401.0
36	38392.5
37	39384.0
38	43932.5
39	48481.0
40	52762.0
41	57043.0
42	69858.0
43	82673.0
44	90087.0
45	97501.0
46	106826.5
47	116152.0
48	126472.0
49	136792.0
50	140176.5
51	143561.0
52	127217.0
53	110873.0
54	97494.0
55	84115.0
56	77291.0
57	70467.0
58	67070.5
59	63674.0
60	59868.5
61	56063.0
62	49125.0
63	42187.0
64	36835.5
65	31484.0
66	26264.5
67	21045.0
68	17910.5
69	14776.0
70	11969.5
71	9163.0
72	8021.0
73	6879.0
74	5322.5
75	3224.5
76	2683.0
77	2074.0
78	1465.0
79	891.5
80	318.0
81	218.5
82	119.0
83	94.5
84	70.0
85	45.0
86	20.0
87	14.5
88	9.0
89	6.5
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1377403.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.8012988014936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.13812831399426	13.294221397184181
2	12.563527407049287	4.9754832037547425
3	5.5573129215094035	3.301260410766272
4	3.1318731250892053	2.4806062223303558
5	1.9605960758145695	1.9411174363120045
6	1.3274328686695167	1.577093692286935
7	0.9308425549994809	1.2902324098083324
8	0.7243175369227339	1.1473942380615145
9	0.5176807393025736	0.9225675902437536
>10	3.661964655109152	14.957262810565734
>50	1.298748222276625	18.99627158919533
>100	1.1782802162446173	32.79386938302255
>500	0.005577217811192236	0.7996313961537506
>1k	0.0037181452074614907	1.5229882203145644
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	4570	0.3317837989317578	TruSeq Adapter, Index 15 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	4331	0.3144323048519569	TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT	2836	0.2058947163611521	TruSeq Adapter, Index 14 (95% over 22bp)
ACAATCGGGAAGGGAACTGTGGTGAGCGCCTGTGCTTGCTCACTGTAGAAA	1595	0.11579762785473822	No Hit
TCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	1558	0.11311141329008285	TruSeq Adapter, Index 15 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4520078727866861E-4	0.0	0.0	0.22462561792010036	0.0
2	1.4520078727866861E-4	0.0	0.0	1.1325661407736152	0.0
3	1.4520078727866861E-4	0.0	0.0	1.6012742821091577	0.0
4	2.1780118091800293E-4	0.0	0.0	2.159861710770196	0.0
5	2.1780118091800293E-4	0.0	0.0	3.6306004851158304	0.0
6	2.1780118091800293E-4	0.0	0.0	4.404665882098413	0.0
7	2.1780118091800293E-4	0.0	0.0	5.206972832206696	0.0
8	2.1780118091800293E-4	0.0	0.0	6.434355087073282	0.0
9	2.1780118091800293E-4	0.0	0.0	6.870973854420239	0.0
10	2.1780118091800293E-4	0.0	0.0	7.933916217693732	0.0
11	2.1780118091800293E-4	0.0	0.0	9.544483350188724	0.0
12	2.1780118091800293E-4	0.0	0.0	10.779996849142917	0.0
13	2.1780118091800293E-4	0.0	0.0	11.253859618426851	0.0
14	2.1780118091800293E-4	0.0	0.0	11.447267067082038	0.0
15	2.1780118091800293E-4	0.0	0.0	11.836622978169787	0.0
16	2.1780118091800293E-4	0.0	0.0	12.65105419401584	0.0
17	2.1780118091800293E-4	0.0	0.0	13.600376941243775	0.0
18	2.1780118091800293E-4	0.0	0.0	14.69141565685569	0.0
19	2.1780118091800293E-4	0.0	0.0	15.297483742956855	0.0
20	2.1780118091800293E-4	0.0	0.0	15.887216740489167	0.0
21	2.1780118091800293E-4	0.0	0.0	16.645673052839292	0.0
22	2.1780118091800293E-4	0.0	0.0	17.438033749018988	0.0
23	2.1780118091800293E-4	0.0	0.0	18.202951496403013	0.0
24	2.9040157455733723E-4	0.0	0.0	18.815408417144436	0.0
25	2.9040157455733723E-4	0.0	0.0	19.354321139129215	0.0
26	2.9040157455733723E-4	0.0	0.0	19.84459159737564	0.0
27	2.9040157455733723E-4	0.0	0.0	20.374429270155503	0.0
28	2.9040157455733723E-4	0.0	0.0	20.875807588628746	0.0
29	2.9040157455733723E-4	0.0	0.0	21.412469698410703	0.0
30	2.9040157455733723E-4	0.0	0.0	22.037704288432653	0.0
31	2.9040157455733723E-4	0.0	0.0	22.581336036003986	0.0
32	2.9040157455733723E-4	0.0	0.0	23.09454821864044	0.0
33	3.6300196819667155E-4	0.0	0.0	23.598031948529226	0.0
34	3.6300196819667155E-4	0.0	0.0	24.103330688258993	0.0
35	3.6300196819667155E-4	0.0	0.0	24.67302597714685	0.0
36	5.082027554753402E-4	0.0	0.0	25.1995240318193	0.0
37	5.082027554753402E-4	0.0	0.0	25.756441651426634	0.0
38	5.082027554753402E-4	0.0	0.0	26.29027234585666	0.0
39	5.082027554753402E-4	0.0	0.0	27.014243471228102	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGACA	30	2.165816E-6	45.000004	40
ACGTTAG	55	1.8189894E-12	45.000004	1
CGCGACT	30	2.165816E-6	45.000004	35
TCGTCCG	30	2.165816E-6	45.000004	1
CCGGTGC	30	2.165816E-6	45.000004	14
TTCGCTA	30	2.165816E-6	45.000004	9
CGATCTC	30	2.165816E-6	45.000004	11
AGCGTAC	40	6.8175723E-9	45.0	6
AACCGAT	20	7.033775E-4	45.0	28
TCGTTAA	20	7.033775E-4	45.0	30
GTCGCAC	25	3.8912098E-5	45.0	26
TACCCGT	25	3.8912098E-5	45.0	36
CGTATGA	20	7.033775E-4	45.0	34
TCCGATA	25	3.8912098E-5	45.0	18
GCGAAAA	20	7.033775E-4	45.0	44
ACTTCGA	25	3.8912098E-5	45.0	43
TGACGAT	25	3.8912098E-5	45.0	29
CGTTCGA	45	3.8562575E-10	45.0	31
TCGATAG	85	0.0	45.0	1
CGTTCAC	20	7.033775E-4	45.0	41
>>END_MODULE