##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935042.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2437449
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.38412947306795	33.0	31.0	34.0	31.0	34.0
2	32.55643543721325	34.0	31.0	34.0	31.0	34.0
3	32.561949809001135	34.0	31.0	34.0	31.0	34.0
4	36.05060700757226	37.0	35.0	37.0	35.0	37.0
5	35.971406581224876	37.0	35.0	37.0	35.0	37.0
6	35.911523482132345	37.0	35.0	37.0	35.0	37.0
7	36.16848721757871	37.0	35.0	37.0	35.0	37.0
8	35.94197006788655	37.0	36.0	37.0	35.0	37.0
9	37.679496883832236	39.0	38.0	39.0	35.0	39.0
10	37.49584175915065	39.0	37.0	39.0	35.0	39.0
11	37.408813476712744	39.0	37.0	39.0	35.0	39.0
12	37.149642105332255	39.0	37.0	39.0	34.0	39.0
13	37.00810109257671	39.0	37.0	39.0	33.0	39.0
14	37.922362273015764	40.0	37.0	41.0	33.0	41.0
15	38.05213729600086	40.0	37.0	41.0	33.0	41.0
16	38.19017997915034	40.0	37.0	41.0	33.0	41.0
17	38.287101801924884	40.0	37.0	41.0	34.0	41.0
18	38.28409209792697	40.0	37.0	41.0	34.0	41.0
19	38.281832358338576	40.0	37.0	41.0	34.0	41.0
20	38.220658155309096	40.0	37.0	41.0	34.0	41.0
21	38.12791652256109	40.0	36.0	41.0	34.0	41.0
22	38.143650185091055	40.0	36.0	41.0	34.0	41.0
23	37.987097986460434	40.0	36.0	41.0	34.0	41.0
24	37.782552578536006	40.0	35.0	41.0	33.0	41.0
25	37.728134209167045	40.0	35.0	41.0	33.0	41.0
26	37.86503225298252	40.0	36.0	41.0	34.0	41.0
27	37.88994764608408	40.0	36.0	41.0	34.0	41.0
28	37.84792789510673	40.0	36.0	41.0	34.0	41.0
29	37.868448939854744	40.0	36.0	41.0	34.0	41.0
30	37.792321398314385	40.0	36.0	41.0	34.0	41.0
31	37.653242385789405	40.0	35.0	41.0	33.0	41.0
32	37.61896474551878	40.0	35.0	41.0	33.0	41.0
33	37.55451334571513	40.0	35.0	41.0	33.0	41.0
34	37.35540641055464	40.0	35.0	41.0	33.0	41.0
35	37.318896108185236	40.0	35.0	41.0	33.0	41.0
36	37.27372059887202	40.0	35.0	41.0	33.0	41.0
37	37.18508489818659	39.0	35.0	41.0	32.0	41.0
38	37.11232686304411	39.0	35.0	41.0	32.0	41.0
39	37.0419237489687	39.0	35.0	41.0	32.0	41.0
40	36.95839256534188	39.0	35.0	41.0	32.0	41.0
41	36.877277432266276	39.0	35.0	41.0	32.0	41.0
42	36.86107196499291	39.0	35.0	41.0	32.0	41.0
43	36.79843885964383	39.0	35.0	41.0	32.0	41.0
44	36.684461090262815	39.0	35.0	41.0	31.0	41.0
45	36.66727426912317	39.0	35.0	41.0	32.0	41.0
46	36.59413345674104	39.0	35.0	41.0	31.0	41.0
47	36.52556709904494	38.0	35.0	41.0	31.0	41.0
48	36.4375619756557	38.0	35.0	41.0	31.0	41.0
49	36.37547000983405	38.0	35.0	40.0	31.0	41.0
50	36.26395013803366	38.0	35.0	40.0	31.0	41.0
51	35.244766967431936	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	17.0
9	30.0
10	45.0
11	40.0
12	32.0
13	50.0
14	61.0
15	123.0
16	192.0
17	376.0
18	621.0
19	1126.0
20	1862.0
21	2879.0
22	4295.0
23	5990.0
24	8289.0
25	10951.0
26	13851.0
27	17032.0
28	20652.0
29	25197.0
30	32387.0
31	42083.0
32	57072.0
33	87467.0
34	172842.0
35	200594.0
36	166802.0
37	268640.0
38	485047.0
39	810069.0
40	734.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.932118374579325	22.03607131882554	25.673808969951782	10.358001336643351
2	31.072978347444398	24.321370416365635	25.97465629024443	18.630994945945538
3	32.70271501065253	24.083744931688827	26.046986008732905	17.166554048925743
4	29.119911842258034	26.010841662738382	25.11121258331969	19.758033911683896
5	25.409064969154226	30.016422907720326	24.804990791602204	19.769521331523244
6	27.563366454026323	33.235895397196	23.720906570763123	15.479831578014554
7	81.83908668448038	5.987079114270698	7.191945349420645	4.981888851828285
8	83.38746779932626	4.4286054805659525	7.047901309935101	5.136025410172684
9	77.71280547818643	6.862584612026754	9.124416551895035	6.300193357891796
10	43.822947680136075	25.980400000164106	16.12878874593889	14.067863573760928
11	34.45938766308546	23.221532019746874	22.59234962454599	19.726730692621672
12	30.151030852337833	22.11968332465623	27.906224909731442	19.823060913274492
13	28.164938015113343	22.882406975489538	29.44693406918463	19.50572094021249
14	21.82515408527522	25.646321215336197	31.48299718271028	21.0455275166783
15	19.786875540780546	24.62053565018181	35.01164537186214	20.580943437175506
16	25.693296557179245	25.35589462589781	29.008155657820943	19.942653159101994
17	26.761708655237506	23.21689602531171	26.308242757079224	23.71315256237156
18	26.017857194140266	23.220834569256628	29.886984302030523	20.87432393457258
19	26.66406558660304	25.42112675998554	26.125879967129567	21.78892768628185
20	25.99619520244321	28.187297457300648	26.251954399866417	19.564552940389728
21	24.661439070109775	25.685829734283672	30.125758528691264	19.526972666915288
22	23.43109537881613	23.90901307063245	29.34666530458688	23.31322624596453
23	22.342785428536146	25.337268595158296	29.64689722738814	22.673048748917413
24	23.02985621442746	25.182516639322504	28.423445987998107	23.364181158251927
25	23.447547005086054	26.159562723158515	26.428491426897548	23.964398844857882
26	21.61038036077883	25.86852073622874	26.912891305623216	25.608207597369216
27	22.108154878317453	25.563611792492893	28.503201502882725	23.82503182630693
28	20.038573114760556	26.36129002083736	27.614649578309127	25.98548728609296
29	22.69811594006685	25.911680613625148	27.278765627506463	24.111437818801544
30	25.139069576430114	24.66714175353002	27.368777767247643	22.825010902792222
31	23.39749467578604	27.211605247945702	26.578156096804488	22.812743979463775
32	25.950204496586387	24.95949658844144	25.70658914299335	23.38370977197882
33	25.83319692022274	25.110843344824858	26.860090200861634	22.195869534090765
34	21.219726033242132	25.71532778737114	30.18175970040809	22.883186478978637
35	21.8912477758509	26.181552926850983	27.442297254219472	24.484902043078645
36	25.919557701514982	26.53692446488111	25.631141410548487	21.912376423055417
37	23.110842524294867	28.680846245398367	26.442358383703617	21.76595284660315
38	22.668371727982823	27.184691864322087	27.04150938132449	23.105427026370602
39	22.601375454419763	25.02169276157163	28.260611811775345	24.116319972233267
40	24.349432541973187	23.545436232717073	28.24469352999796	23.860437695311777
41	21.62465758257916	24.751369156852103	25.793729427774693	27.83024383279404
42	22.626729831065184	24.165715877542464	27.74737030395303	25.46018398743933
43	22.67144871543979	23.635776584453662	28.560761681577745	25.1320130185288
44	22.426602566863963	24.219214432794285	28.190907789250154	25.163275211091594
45	21.62359089359408	23.969691263283867	27.15351172475814	27.253206118363916
46	21.923863842894765	24.679490729857324	27.596926130556987	25.799719296690927
47	20.24916213631547	26.05375538113823	29.21070348548831	24.48637899705799
48	21.296527640168062	25.27441599803729	28.523304487601585	24.90575187419306
49	23.488368372015167	23.85896074133243	29.299033538753015	23.35363734789938
50	20.91879665995063	25.092422446582475	28.158250695706865	25.830530197760037
51	20.3095531434709	25.106658641883378	26.699963773601006	27.883824441044712
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	664.0
1	1400.0
2	2136.0
3	3229.5
4	4323.0
5	3368.5
6	2414.0
7	2472.5
8	2531.0
9	2604.0
10	2677.0
11	2899.0
12	3121.0
13	3251.0
14	3381.0
15	3406.5
16	3432.0
17	3934.0
18	4436.0
19	4311.5
20	4187.0
21	4990.0
22	5793.0
23	7608.0
24	9423.0
25	11416.5
26	15280.0
27	17150.0
28	19876.5
29	22603.0
30	26970.5
31	31338.0
32	36877.0
33	42416.0
34	50794.5
35	59173.0
36	64355.0
37	69537.0
38	75113.5
39	80690.0
40	92884.5
41	105079.0
42	115386.0
43	125693.0
44	139174.0
45	152655.0
46	172995.5
47	193336.0
48	217742.5
49	242149.0
50	242794.0
51	243439.0
52	216186.0
53	188933.0
54	172064.0
55	155195.0
56	148697.5
57	142200.0
58	133840.5
59	125481.0
60	114779.5
61	104078.0
62	93017.0
63	81956.0
64	73540.0
65	65124.0
66	54910.0
67	44696.0
68	37869.0
69	31042.0
70	26523.0
71	22004.0
72	17400.0
73	12796.0
74	10652.5
75	6362.5
76	4216.0
77	3110.5
78	2005.0
79	1609.5
80	1214.0
81	850.0
82	486.0
83	331.0
84	176.0
85	140.0
86	104.0
87	64.5
88	25.0
89	18.0
90	11.0
91	9.0
92	7.0
93	5.0
94	3.0
95	2.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2437449.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.995647863102153
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.19600429393728	12.76431635375133
2	12.573955343741408	4.7770085591216676
3	5.517003761137674	3.1439718211794423
4	3.0249891603648207	2.2984651551996476
5	1.8916756232808614	1.7966802005528766
6	1.2542691565459851	1.4295393133978607
7	0.9153721605805853	1.2171661058253058
8	0.6838028023262768	1.0391421792633924
9	0.5174697858776328	0.8846706449093746
>10	3.8628079944520395	15.356446907118126
>50	1.2439929234844322	17.539788898700547
>100	1.3094279393693362	35.41297467568936
>500	0.005273745658050577	0.6459407849718082
>1k	0.003515830438700385	1.186777925577929
>5k	4.394788048375481E-4	0.5071104747413568
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	6425	0.2635952588136203	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	5724	0.23483568271582297	No Hit
CCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	4805	0.19713232974310438	No Hit
CTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGCT	3514	0.14416711898382284	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	2489	0.10211495707192232	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.20529988524888E-5	0.0	0.0	0.1523313923696455	0.0
2	8.20529988524888E-5	0.0	0.0	0.8146631991069352	0.0
3	8.20529988524888E-5	0.0	0.0	1.1899325893587926	0.0
4	8.20529988524888E-5	0.0	0.0	1.644383123503302	0.0
5	8.20529988524888E-5	0.0	0.0	2.9171892417031087	0.0
6	8.20529988524888E-5	0.0	0.0	3.605531849076637	0.0
7	8.20529988524888E-5	0.0	0.0	4.322757112046242	0.0
8	8.20529988524888E-5	0.0	0.0	5.412954281299834	0.0
9	8.20529988524888E-5	0.0	0.0	5.782685094129149	0.0
10	1.2307949827873322E-4	0.0	0.0	6.68063208707136	0.0
11	1.2307949827873322E-4	0.0	0.0	8.075574094063096	0.0
12	1.2307949827873322E-4	0.0	0.0	9.132170560286594	0.0
13	1.2307949827873322E-4	0.0	0.0	9.546045886498549	0.0
14	1.2307949827873322E-4	0.0	0.0	9.715649435126643	0.0
15	1.2307949827873322E-4	0.0	0.0	10.056210406863897	0.0
16	1.2307949827873322E-4	0.0	0.0	10.730932216427913	0.0
17	1.641059977049776E-4	0.0	0.0	11.525861669310824	0.0
18	1.641059977049776E-4	0.0	0.0	12.47816877399281	0.0
19	1.641059977049776E-4	0.0	0.0	13.023575057365303	0.0
20	1.641059977049776E-4	0.0	0.0	13.606028269719694	0.0
21	1.641059977049776E-4	0.0	0.0	14.295109354082896	0.0
22	1.641059977049776E-4	0.0	0.0	15.053607275475303	0.0
23	1.641059977049776E-4	0.0	0.0	15.725621336077186	0.0
24	1.641059977049776E-4	0.0	0.0	16.294412724122637	0.0
25	1.641059977049776E-4	0.0	0.0	16.800433568045936	0.0
26	1.641059977049776E-4	0.0	0.0	17.281264141321522	0.0
27	1.641059977049776E-4	0.0	0.0	17.865440466651815	0.0
28	1.641059977049776E-4	0.0	0.0	18.364979123665766	0.0
29	1.641059977049776E-4	0.0	0.0	18.946940018027043	0.0
30	1.641059977049776E-4	0.0	0.0	19.612430865220155	0.0
31	1.641059977049776E-4	0.0	0.0	20.132154559951818	0.0
32	2.0513249713122203E-4	0.0	0.0	20.622544307593717	0.0
33	2.0513249713122203E-4	0.0	0.0	21.134678099931527	0.0
34	2.0513249713122203E-4	0.0	0.0	21.6736432228941	0.0
35	2.0513249713122203E-4	0.0	0.0	22.24354232642406	0.0
36	2.0513249713122203E-4	0.0	0.0	22.73951167798793	0.0
37	2.0513249713122203E-4	0.0	0.0	23.273717727017058	0.0
38	2.0513249713122203E-4	0.0	0.0	23.91373111806647	0.0
39	2.0513249713122203E-4	0.0	0.0	24.937260225752414	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCGTAA	35	1.2126839E-7	45.000004	36
ACGTTAC	20	7.034844E-4	45.0	30
ACCGCGT	55	1.8189894E-12	45.0	35
CATTACG	20	7.034844E-4	45.0	26
CGTTACA	20	7.034844E-4	45.0	31
ACTCGAT	25	3.8920974E-5	45.0	29
CGCGTAC	20	7.034844E-4	45.0	28
TAATCCG	30	2.1665073E-6	44.999996	1
TATAGCG	160	0.0	43.59375	1
CGTAAGG	415	0.0	43.373493	2
TAGCACG	80	0.0	42.1875	1
GCGTAAG	225	0.0	42.0	1
TCGATAG	60	3.6379788E-12	41.249996	1
CGCGTAA	60	3.6379788E-12	41.249996	31
TCGACTA	55	6.184564E-11	40.909092	1
CGAATAT	170	0.0	39.705883	14
ATTTTCG	40	3.4603545E-7	39.375	37
TCTATCG	40	3.4603545E-7	39.375	43
CCTTACG	35	6.25117E-6	38.57143	15
CGCATAC	35	6.25117E-6	38.57143	13
>>END_MODULE