##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935041.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1581850
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.30237000979865	33.0	31.0	34.0	30.0	34.0
2	32.47019881783987	34.0	31.0	34.0	31.0	34.0
3	32.48498466984859	34.0	31.0	34.0	30.0	34.0
4	36.006344470082496	37.0	35.0	37.0	35.0	37.0
5	35.92663337231723	37.0	35.0	37.0	35.0	37.0
6	35.85528147422322	37.0	35.0	37.0	35.0	37.0
7	36.084057906881185	37.0	35.0	37.0	35.0	37.0
8	35.66022758162911	37.0	35.0	37.0	35.0	37.0
9	37.495251130006004	39.0	37.0	39.0	35.0	39.0
10	37.38595758131302	39.0	37.0	39.0	34.0	39.0
11	37.33638145209723	39.0	37.0	39.0	34.0	39.0
12	37.16926573316054	39.0	37.0	39.0	34.0	39.0
13	37.061407845244496	39.0	37.0	39.0	33.0	39.0
14	38.23377184941682	40.0	37.0	41.0	33.0	41.0
15	38.26096279672535	40.0	37.0	41.0	34.0	41.0
16	38.383420046148494	40.0	37.0	41.0	34.0	41.0
17	38.329887157442236	40.0	37.0	41.0	34.0	41.0
18	38.3408730284161	40.0	37.0	41.0	34.0	41.0
19	38.323460505104784	40.0	37.0	41.0	34.0	41.0
20	38.202005879192086	40.0	37.0	41.0	34.0	41.0
21	38.20467490596454	40.0	37.0	41.0	34.0	41.0
22	38.12907165660461	40.0	37.0	41.0	34.0	41.0
23	37.88606947561399	40.0	36.0	41.0	33.0	41.0
24	37.64436893510763	40.0	36.0	41.0	33.0	41.0
25	37.61400575275785	39.0	36.0	41.0	33.0	41.0
26	37.76698802035591	40.0	36.0	41.0	33.0	41.0
27	37.74194266207289	40.0	36.0	41.0	33.0	41.0
28	37.70111009261308	40.0	36.0	41.0	33.0	41.0
29	37.743423207004454	40.0	36.0	41.0	33.0	41.0
30	37.63313841388248	40.0	36.0	41.0	33.0	41.0
31	37.615438884850015	40.0	36.0	41.0	33.0	41.0
32	37.4169655782786	40.0	35.0	41.0	33.0	41.0
33	37.32639694029143	39.0	35.0	41.0	33.0	41.0
34	37.25276100768088	39.0	35.0	41.0	33.0	41.0
35	37.16684894269368	39.0	35.0	41.0	32.0	41.0
36	37.1020204191295	39.0	35.0	41.0	32.0	41.0
37	37.03331858267219	39.0	35.0	41.0	32.0	41.0
38	36.95540411543446	39.0	35.0	41.0	32.0	41.0
39	36.89733034105636	39.0	35.0	41.0	32.0	41.0
40	36.854914182760695	39.0	35.0	41.0	31.0	41.0
41	36.77367386288207	39.0	35.0	41.0	31.0	41.0
42	36.82173278123716	39.0	35.0	41.0	32.0	41.0
43	36.731329772102285	39.0	35.0	41.0	31.0	41.0
44	36.67220216834719	39.0	35.0	41.0	31.0	41.0
45	36.698859563169705	39.0	35.0	41.0	31.0	41.0
46	36.59252836868224	39.0	35.0	41.0	31.0	41.0
47	36.494257989063435	39.0	35.0	41.0	31.0	41.0
48	36.44416031861428	39.0	35.0	40.0	31.0	41.0
49	36.411551032019474	39.0	35.0	40.0	31.0	41.0
50	36.32719663684926	38.0	35.0	40.0	31.0	41.0
51	35.41319025192022	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	12.0
9	17.0
10	23.0
11	29.0
12	20.0
13	27.0
14	39.0
15	56.0
16	90.0
17	182.0
18	323.0
19	704.0
20	1301.0
21	2414.0
22	3987.0
23	5578.0
24	7227.0
25	8458.0
26	9995.0
27	11470.0
28	13714.0
29	16615.0
30	20938.0
31	27449.0
32	36623.0
33	51089.0
34	96490.0
35	136887.0
36	114422.0
37	180512.0
38	324083.0
39	510638.0
40	436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.05939248348453	20.562632360843317	24.17315168947751	10.204823466194647
2	29.535290956791098	29.630622372538483	23.688782122198692	17.145304548471728
3	30.71795682270759	28.48664538356987	25.359168062711383	15.436229731011158
4	27.801182160128963	24.573252836868225	29.1580111894301	18.467553813572714
5	28.92733192148434	29.102569775895315	22.361475487562032	19.608622815058318
6	24.861459683282234	37.82261276353637	22.74292758478996	14.57299996839144
7	76.98530201978696	6.380250971963207	12.074469766412745	4.55997724183709
8	76.42665233745298	8.835793532888706	8.506179473401398	6.231374656256914
9	71.22223978253311	7.594651831716029	9.82469892846983	11.358409457281033
10	41.65963903024939	26.04836109618485	17.06223725384834	15.229762619717418
11	33.51164775421184	24.16291051616778	22.308499541675886	20.016942187944494
12	28.26563833486108	21.768814995100673	28.595315611467587	21.37023105857066
13	24.871511205234377	24.45124379682018	31.590605936087492	19.08663906185795
14	20.248506495558996	30.229351708442646	27.786768656952304	21.735373139046054
15	18.354268735973704	25.71084489679805	35.33723172235042	20.59765464487783
16	20.83250624269052	25.182539431678098	29.969276480070807	24.015677845560578
17	21.124885418971456	25.79998103486424	27.845370926446883	25.229762619717423
18	20.737490912539116	26.56737364478301	29.293359041628474	23.401776401049403
19	23.414040522173405	26.52546069475614	26.354142301735312	23.706356481335146
20	24.899073869203782	27.539147201062047	28.25716724088883	19.304611688845338
21	23.729746815437622	29.061415431298798	27.433195309289754	19.77564244397383
22	20.818914562063405	24.971836773398238	28.040901476119735	26.168347188418622
23	21.399121282043176	29.081708126560674	26.96886556879603	22.55030502260012
24	22.605872870373297	24.93447545595347	27.954546891298165	24.505104782375067
25	20.805006795840313	29.359357714068967	26.049056484496003	23.786579005594717
26	19.98710370768404	28.33574612004931	26.675791004203937	25.00135916806271
27	22.87821221986914	29.043461769447166	25.37193792078895	22.706388089894745
28	19.510193760470333	26.73850238644625	29.335019123178558	24.41628472990486
29	21.182855517274078	25.634605051047828	27.65748964819673	25.525049783481368
30	22.036602712014414	30.539684546575213	25.622340929923826	21.80137181148655
31	25.71729304295603	26.88851661029807	22.046338148370577	25.34785219837532
32	24.43619812245156	28.655751177418843	25.13879318519455	21.769257514935045
33	22.82296045769194	28.96229098839966	24.03818313999431	24.17656541391409
34	22.572620665676265	25.393811044030723	25.581945190757654	26.451623099535354
35	22.253121345260297	26.194582292884917	26.208742927584787	25.34355343427
36	23.375414862344723	27.392736353004395	25.620634067705534	23.61121471694535
37	22.605430350538928	27.84145146505674	28.03299933622025	21.52011884818409
38	23.706799001169518	28.002402250529446	24.53791446723773	23.75288428106331
39	22.320700445680693	25.188861143597684	28.3681132850776	24.122325125644025
40	22.949457913202895	25.481809273951384	27.6319499320416	23.936782880804124
41	20.7070834782059	26.265575117741886	25.822992066251544	27.204349337800675
42	23.324588298511237	24.41963523722224	28.42993962765117	23.825836836615355
43	23.355817555394	24.156525587128993	28.590827196004675	23.896829661472328
44	22.22378860195341	24.99971552296362	27.594778265954425	25.18171760912855
45	22.532098492271707	23.47099914656889	26.058033315421813	27.938869045737587
46	24.428169548313683	26.427600594240918	25.87799095995196	23.26623889749344
47	20.377848721433764	24.91456206340677	31.34475455953472	23.36283465562474
48	21.55077915099409	24.386130164048424	27.953029680437464	26.110061004520023
49	21.511268451496665	22.719663684925877	32.59468344027563	23.17438442330183
50	21.119006226886242	23.663684925877927	29.46265448683503	25.754654360400796
51	20.63330910010431	23.02974365458166	26.641400891361382	29.69554635395265
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	843.0
1	1591.0
2	2339.0
3	2819.0
4	3299.0
5	2598.0
6	1897.0
7	1914.0
8	1931.0
9	2083.0
10	2235.0
11	2255.0
12	2275.0
13	2569.5
14	2864.0
15	2758.5
16	2653.0
17	2954.5
18	3256.0
19	3723.5
20	4191.0
21	4371.5
22	4552.0
23	5023.5
24	5495.0
25	8042.0
26	11185.5
27	11782.0
28	16282.0
29	20782.0
30	23765.5
31	26749.0
32	26623.5
33	26498.0
34	30853.0
35	35208.0
36	36725.0
37	38242.0
38	43132.5
39	48023.0
40	55263.5
41	62504.0
42	69263.0
43	76022.0
44	90112.0
45	104202.0
46	148373.5
47	192545.0
48	182119.0
49	171693.0
50	168439.5
51	165186.0
52	140715.0
53	116244.0
54	102619.5
55	88995.0
56	81466.5
57	73938.0
58	71000.0
59	68062.0
60	64197.5
61	60333.0
62	53026.0
63	45719.0
64	40687.5
65	35656.0
66	29423.0
67	23190.0
68	18933.5
69	14677.0
70	13069.5
71	11462.0
72	8796.0
73	6130.0
74	5226.0
75	3356.5
76	2391.0
77	1783.0
78	1175.0
79	974.0
80	773.0
81	640.5
82	508.0
83	383.0
84	258.0
85	186.5
86	115.0
87	75.0
88	35.0
89	21.0
90	7.0
91	5.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1581850.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.302920027813418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.55108663607945	13.039332231281373
2	12.630427243255845	4.876082539873669
3	5.545627976689631	3.2114044001413404
4	3.1250645101060766	2.4129148128134204
5	1.8732273367361914	1.8079378737466307
6	1.3011705888004046	1.5069835090894261
7	0.9137511581630654	1.234664587194048
8	0.6838604497799872	1.0560402857830085
9	0.514903249401251	0.8945222620728255
>10	3.3970469012824696	13.878697885103964
>50	1.3484927299223677	19.19563201584846
>100	1.0980953846870696	29.25621886418881
>500	0.00895456822300798	1.184017802902408
>1k	0.006964664173450651	2.7451672022848426
>5k	3.316506749262215E-4	0.5716190841911652
>10k+	9.949520247786645E-4	3.1287646434846925
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGC	17873	1.129879571387932	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTC	16172	1.022347251635743	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCG	14828	0.9373834434364826	No Hit
GCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	8929	0.5644656572999969	No Hit
CCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	4522	0.2858678130037614	No Hit
CTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGCT	3680	0.23263899864083196	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGTACGTT	3528	0.22302999652305844	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCT	3390	0.21430603407402724	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTC	2590	0.16373233871732465	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTA	2537	0.1603818313999431	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	2492	0.15753706103612858	No Hit
GAACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCT	2333	0.14748553908398393	No Hit
TCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	2317	0.14647406517684988	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGC	1745	0.11031387299680753	No Hit
ACTGTCTCTTATACACATCTGACGCCAGTACGTTCGTATGCCGTCTTCTGC	1697	0.10727945127540538	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1589	0.10045200240225052	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8965135758763473E-4	0.0	0.0	0.24566172519518287	0.0
2	1.8965135758763473E-4	0.0	0.0	1.4061383822739197	0.0
3	1.8965135758763473E-4	0.0	0.0	1.8891803900496253	0.0
4	1.8965135758763473E-4	0.0	0.0	2.6446881815595664	0.0
5	1.8965135758763473E-4	0.0	0.0	5.328823845497361	0.0
6	1.8965135758763473E-4	0.0	0.0	6.213737080001264	0.0
7	1.8965135758763473E-4	0.0	0.0	7.304864557322123	0.0
8	1.8965135758763473E-4	0.0	0.0	8.751588330119796	0.0
9	1.8965135758763473E-4	0.0	0.0	9.20061952776812	0.0
10	1.8965135758763473E-4	0.0	0.0	11.286847678351297	0.0
11	2.5286847678351296E-4	0.0	0.0	12.922274551948668	0.0
12	2.5286847678351296E-4	0.0	0.0	15.491165407592376	0.0
13	2.5286847678351296E-4	0.0	0.0	16.059108006448145	0.0
14	2.5286847678351296E-4	0.0	0.0	16.301924961279514	0.0
15	2.5286847678351296E-4	0.0	0.0	16.87195372506875	0.0
16	2.5286847678351296E-4	0.0	0.0	17.67626513259791	0.0
17	2.5286847678351296E-4	0.0	0.0	18.614091095868762	0.0
18	2.5286847678351296E-4	0.0	0.0	19.59737016784145	0.0
19	2.5286847678351296E-4	0.0	0.0	20.507696684262097	0.0
20	2.5286847678351296E-4	0.0	0.0	21.140752915889625	0.0
21	2.5286847678351296E-4	0.0	0.0	21.84752030849954	0.0
22	2.5286847678351296E-4	0.0	0.0	22.636027436229732	0.0
23	2.5286847678351296E-4	0.0	0.0	23.31181844043367	0.0
24	2.5286847678351296E-4	0.0	0.0	23.911559250244967	0.0
25	2.5286847678351296E-4	0.0	0.0	24.442772702847932	0.0
26	2.5286847678351296E-4	0.0	0.0	24.921389512279926	0.0
27	2.5286847678351296E-4	0.0	0.0	25.422638050384045	0.0
28	3.1608559597939124E-4	0.0	0.0	25.897335398425895	0.0
29	3.1608559597939124E-4	0.0	0.0	26.386888769478777	0.0
30	3.1608559597939124E-4	0.0	0.0	26.91930334734646	0.0
31	3.1608559597939124E-4	0.0	0.0	27.420867971046558	0.0
32	3.1608559597939124E-4	0.0	0.0	27.921610772197113	0.0
33	3.1608559597939124E-4	0.0	0.0	28.398710370768406	0.0
34	3.1608559597939124E-4	0.0	0.0	28.883396023643204	0.0
35	3.1608559597939124E-4	0.0	0.0	29.383506653601795	0.0
36	3.1608559597939124E-4	0.0	0.0	29.862249897272182	0.0
37	3.1608559597939124E-4	0.0	0.0	30.32468312419003	0.0
38	3.7930271517526946E-4	0.0	0.0	30.79065650978285	0.0
39	4.425198343711477E-4	0.0	0.0	31.26592281189746	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	20	7.034092E-4	45.000004	28
GTCGATC	20	7.034092E-4	45.000004	23
CACGGTA	20	7.034092E-4	45.000004	36
CGGTTAC	20	7.034092E-4	45.000004	45
GCTATCG	20	7.034092E-4	45.000004	14
CGACATA	20	7.034092E-4	45.000004	11
CGTACTT	20	7.034092E-4	45.000004	39
CGTACCC	20	7.034092E-4	45.000004	28
TACTACG	20	7.034092E-4	45.000004	17
CGTATTA	45	3.8562575E-10	45.0	18
CCGGATA	25	3.8914743E-5	45.0	27
CGTAGTA	25	3.8914743E-5	45.0	34
TCGTCGG	25	3.8914743E-5	45.0	1
CGTACTA	25	3.8914743E-5	45.0	42
CTCGAAT	45	3.8562575E-10	45.0	43
TTACGCG	35	1.21232E-7	45.0	1
CGGTCTA	35	1.21232E-7	45.0	31
TCGACGA	25	3.8914743E-5	45.0	39
TGTAACG	35	1.21232E-7	45.0	1
TCGAACG	25	3.8914743E-5	45.0	1
>>END_MODULE