FastQCFastQC Report
Sat 14 Jan 2017
SRR2935039.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935039.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2153253
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGC156070.724810321871141No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC132140.6136761448840429RNA PCR Primer, Index 4 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCG129800.6028088663988858No Hit
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC108980.5061179526976162RNA PCR Primer, Index 48 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC85780.3983739950669986RNA PCR Primer, Index 4 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGCT58100.26982430768702054TruSeq Adapter, Index 4 (96% over 27bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCAATCT34370.159618957920876No Hit
TCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC32210.1495876239345771RNA PCR Primer, Index 48 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCT30340.14090308941866098TruSeq Adapter, Index 4 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC22900.10635071679918709TruSeq Adapter, Index 4 (96% over 26bp)
GAATGATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTC22730.10556121366137652No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCT21590.10026689850194102TruSeq Adapter, Index 4 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTA21560.10012757441879797No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAT253.8919447E-545.00000440
TCCGCGA253.8919447E-545.00000414
TAGCGAT253.8919447E-545.00000431
CGCTATA253.8919447E-545.00000421
ATCGTCC207.0346624E-445.020
TACGCGC207.0346624E-445.025
GTTAGCG1650.042.2727281
AACGTAG800.042.18751
GACCCGT650.041.5384620
TCGCAAG603.6379788E-1241.2499961
AGTCGAA950.040.26315739
TAGGACG1750.039.8571431
GACGTAT850.039.7058839
CGACGGT2900.039.56896628
TCACGAC3250.038.7692325
CACGCGA356.2508334E-638.57142611
CGCGATA356.2508334E-638.57142622
GATATCG356.2508334E-638.5714261
ATCCGGC3200.037.968756
GTCGAAA301.1402531E-437.49999640
Produced by FastQC (version 0.11.5)