##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935039.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2153253
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26551872910429	33.0	31.0	34.0	30.0	34.0
2	32.430933568883916	34.0	31.0	34.0	30.0	34.0
3	32.44583427957606	34.0	31.0	34.0	30.0	34.0
4	35.97255547768887	37.0	35.0	37.0	35.0	37.0
5	35.89599410752011	37.0	35.0	37.0	35.0	37.0
6	35.82784512549152	37.0	35.0	37.0	35.0	37.0
7	36.11989951947124	37.0	35.0	37.0	35.0	37.0
8	35.81013285480155	37.0	35.0	37.0	35.0	37.0
9	37.55594303131123	39.0	38.0	39.0	35.0	39.0
10	37.34666061071319	39.0	37.0	39.0	34.0	39.0
11	37.383868964770976	39.0	37.0	39.0	35.0	39.0
12	37.307706990307224	39.0	37.0	39.0	34.0	39.0
13	37.25157889017222	39.0	37.0	39.0	34.0	39.0
14	38.42321640791862	40.0	38.0	41.0	34.0	41.0
15	38.44694608575955	40.0	38.0	41.0	34.0	41.0
16	38.53221660436558	40.0	38.0	41.0	34.0	41.0
17	38.51558873945607	40.0	38.0	41.0	34.0	41.0
18	38.52577054345216	40.0	38.0	41.0	34.0	41.0
19	38.49350726551873	40.0	38.0	41.0	34.0	41.0
20	38.4240486371086	40.0	38.0	41.0	34.0	41.0
21	38.41368710504525	40.0	38.0	41.0	34.0	41.0
22	38.349809334992216	40.0	37.0	41.0	34.0	41.0
23	38.10506847082066	40.0	37.0	41.0	34.0	41.0
24	37.90756590145236	40.0	37.0	41.0	33.0	41.0
25	37.89661270644927	40.0	37.0	41.0	33.0	41.0
26	38.072080707654884	40.0	37.0	41.0	34.0	41.0
27	38.079534081689424	40.0	37.0	41.0	34.0	41.0
28	38.05144634652779	40.0	37.0	41.0	34.0	41.0
29	38.07894566964495	40.0	37.0	41.0	34.0	41.0
30	37.93767012051069	40.0	37.0	41.0	33.0	41.0
31	37.89392189399016	40.0	37.0	41.0	33.0	41.0
32	37.80954815806596	40.0	37.0	41.0	33.0	41.0
33	37.70253472304462	40.0	37.0	41.0	33.0	41.0
34	37.600103192704246	40.0	36.0	41.0	33.0	41.0
35	37.54401271007169	40.0	36.0	41.0	33.0	41.0
36	37.50038244460823	40.0	36.0	41.0	33.0	41.0
37	37.41289435101216	40.0	36.0	41.0	33.0	41.0
38	37.37823051912618	40.0	36.0	41.0	33.0	41.0
39	37.30452715031629	40.0	36.0	41.0	32.0	41.0
40	37.231722421842676	40.0	36.0	41.0	32.0	41.0
41	37.18649364473195	40.0	35.0	41.0	32.0	41.0
42	37.23995229543393	40.0	36.0	41.0	32.0	41.0
43	37.164971789195235	39.0	35.0	41.0	32.0	41.0
44	37.04792887784204	39.0	35.0	41.0	32.0	41.0
45	37.02448713643961	39.0	35.0	41.0	32.0	41.0
46	36.87086608029804	39.0	35.0	41.0	31.0	41.0
47	36.76094123635263	39.0	35.0	41.0	31.0	41.0
48	36.74157193790047	39.0	35.0	41.0	31.0	41.0
49	36.69787015274099	39.0	35.0	41.0	31.0	41.0
50	36.6004821542104	39.0	35.0	41.0	31.0	41.0
51	35.550119749049465	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	10.0
9	28.0
10	36.0
11	42.0
12	20.0
13	31.0
14	34.0
15	62.0
16	99.0
17	178.0
18	426.0
19	804.0
20	1569.0
21	2830.0
22	4266.0
23	5829.0
24	7643.0
25	9533.0
26	11708.0
27	13725.0
28	16470.0
29	20441.0
30	26398.0
31	34847.0
32	46526.0
33	64442.0
34	111398.0
35	159605.0
36	155412.0
37	245750.0
38	469510.0
39	742929.0
40	650.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.402910387214135	20.566603181326116	24.39945514995219	10.63103128150756
2	28.573929770444995	27.645752728546064	25.892591348996145	17.887726152012792
3	30.24191769383347	28.267695435696595	25.532392152710344	15.957994717759593
4	27.54181696252136	25.78679792852953	28.495258104830228	18.176127004118882
5	26.945556328030197	29.78089430271315	24.36564583910948	18.907903530147177
6	25.947995892726027	35.61694793876985	23.469188246806112	14.96586792169801
7	79.61906938014252	5.289345933803412	10.334735398023364	4.756849288030715
8	79.41008325542795	6.15236574615245	8.215244562529344	6.222306435890255
9	73.25089062920149	7.442460314695951	9.998546385399209	9.30810267070335
10	33.97766077650884	34.555948604274555	16.83903377819513	14.627356841021468
11	29.006020193632608	25.397805088394165	25.309659385125666	20.286515332847557
12	25.8975141332672	22.420774521154737	29.889613528925768	21.79209781665229
13	24.4922914306865	23.791073320227582	32.89729539445666	18.819339854629252
14	20.500517124555266	29.050418134794192	28.79067160245452	21.658393138196022
15	17.935328547086662	25.78066766887124	34.83645442500254	21.447549359039556
16	20.932143134132403	26.693008206652912	28.986932794242016	23.387915864972673
17	20.89094964688311	25.423394278331436	28.602444766128272	25.083211308657184
18	21.38225280540652	24.98387323737619	30.932825822139804	22.701048135077485
19	21.548977291567688	27.00249343667465	27.81688914400677	23.6316401277509
20	22.498424476826457	27.43402656352969	29.335382326182756	20.732166633461095
21	22.924477523077876	28.102178424922663	28.361739191818145	20.611604860181316
22	20.545193713883133	24.67503818640912	28.689057904482198	26.090710195225547
23	18.97008851259002	27.90782132893812	28.783055219242698	24.33903493922916
24	21.344867509763134	25.130535055564767	28.552752509807256	24.971844924864843
25	19.335349817230025	29.129507772658393	26.602401111248884	24.932741298862698
26	20.328358999151515	27.103642721036497	28.116528805486396	24.45146947432559
27	22.200781793871876	27.460405256604776	27.26112537634918	23.07768757317417
28	19.697081578430403	26.38096870177355	29.583146987372128	24.33880273242392
29	22.3594719245718	24.59302274279892	27.630101989872998	25.417403342756284
30	22.32482666923023	29.175298954651403	26.545417561243383	21.95445681487498
31	24.869441723754708	28.25613153679572	22.87334558456438	24.00108115488519
32	23.509360024112354	28.03455980323724	24.42183988597717	24.034240286673235
33	21.236752021244136	29.059706407003727	24.49948984164889	25.204051730103245
34	22.741730767355254	27.353311478028825	26.74748392316184	23.157473831454084
35	21.12598937514542	26.889687370689835	26.50064808919342	25.483675164971327
36	23.786661390928053	28.42638556639652	25.32865390179417	22.458299140881262
37	21.054191030965708	28.440364416071866	27.371284284754278	23.13416026820815
38	20.584018691718995	28.298114527182825	25.61812290520436	25.49974387589382
39	20.927452556666587	27.276636790939108	26.12674869139855	25.66916196099576
40	21.103836845925677	27.226155031480275	28.89061341142913	22.779394711164922
41	20.014879812079677	27.392740193558307	26.341632868966165	26.250747125395858
42	21.376261869831367	24.31892582989551	29.48306585431438	24.82174644595874
43	22.67889560585774	24.45244474290759	26.635885332564264	26.232774318670405
44	21.842161603861694	25.018332727273574	27.203677412733203	25.935828256131536
45	21.70427720291113	24.25330418673514	26.658595158116583	27.38382345223715
46	24.18122719438914	26.0987445506868	26.789931327159415	22.930096927764644
47	19.441468327223973	25.63470247109838	31.15890236771991	23.764926833957738
48	20.509155217710134	24.86424029131737	28.544950361151244	26.081654129821253
49	21.353459161556955	21.929796452158666	31.624523453583947	25.09222093270043
50	21.00282688564697	22.791469465037316	29.663862072873	26.541841576442714
51	19.961425805513798	22.91974050425101	27.24654278898021	29.872290901254985
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1235.0
1	1628.0
2	2021.0
3	3066.0
4	4111.0
5	3146.0
6	2181.0
7	2417.5
8	2654.0
9	2674.0
10	2694.0
11	2834.0
12	2974.0
13	3180.5
14	3387.0
15	3653.5
16	3920.0
17	3910.5
18	3901.0
19	4311.5
20	4722.0
21	5291.0
22	5860.0
23	6197.5
24	6535.0
25	7907.0
26	11841.0
27	14403.0
28	18167.5
29	21932.0
30	25464.0
31	28996.0
32	34167.0
33	39338.0
34	46835.0
35	54332.0
36	57990.5
37	61649.0
38	65655.0
39	69661.0
40	79146.5
41	88632.0
42	102776.0
43	116920.0
44	136757.0
45	156594.0
46	208445.5
47	260297.0
48	261433.0
49	262569.0
50	253589.0
51	244609.0
52	208239.5
53	171870.0
54	149336.5
55	126803.0
56	112182.0
57	97561.0
58	87211.0
59	76861.0
60	70446.5
61	64032.0
62	57034.0
63	50036.0
64	42238.0
65	34440.0
66	27700.0
67	20960.0
68	17405.5
69	13851.0
70	11110.0
71	8369.0
72	6902.5
73	5436.0
74	4735.5
75	2898.0
76	1761.0
77	1536.0
78	1311.0
79	790.0
80	269.0
81	188.0
82	107.0
83	75.0
84	43.0
85	30.5
86	18.0
87	45.5
88	73.0
89	40.5
90	8.0
91	4.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2153253.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.588457372501985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.09270910221632	13.607469239841546
2	12.764883030121737	5.256185002612707
3	5.915885932414046	3.653968960202722
4	3.268816632193682	2.6919956756178047
5	2.1056375218758703	2.1675914180541036
6	1.3833671438592772	1.7088837283119929
7	1.011391552514911	1.4576094306103295
8	0.7601055393854752	1.2519520396992385
9	0.5770223234241233	1.0691999557919654
>10	3.9263399261694296	17.267863887069108
>50	1.3472073841064482	20.269386750573588
>100	0.832017093117354	23.42298050008213
>500	0.008221960463261797	1.1363221229133311
>1k	0.0050245313942155435	1.8840583804059983
>5k	4.567755812923221E-4	0.6765456717899946
>10k+	9.135511625846442E-4	2.4779872364234725
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGC	15607	0.724810321871141	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC	13214	0.6136761448840429	RNA PCR Primer, Index 4 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCG	12980	0.6028088663988858	No Hit
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	10898	0.5061179526976162	RNA PCR Primer, Index 48 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	8578	0.3983739950669986	RNA PCR Primer, Index 4 (96% over 26bp)
CTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGCT	5810	0.26982430768702054	TruSeq Adapter, Index 4 (96% over 27bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCAATCT	3437	0.159618957920876	No Hit
TCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	3221	0.1495876239345771	RNA PCR Primer, Index 48 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCT	3034	0.14090308941866098	TruSeq Adapter, Index 4 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	2290	0.10635071679918709	TruSeq Adapter, Index 4 (96% over 26bp)
GAATGATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTC	2273	0.10556121366137652	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCT	2159	0.10026689850194102	TruSeq Adapter, Index 4 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTA	2156	0.10012757441879797	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3932408314303987E-4	0.0	0.0	0.28528928091589795	0.0
2	1.3932408314303987E-4	0.0	0.0	1.5144527837648432	0.0
3	1.3932408314303987E-4	0.0	0.0	2.0817804503233015	0.0
4	1.3932408314303987E-4	0.0	0.0	2.8801074467329197	0.0
5	1.3932408314303987E-4	0.0	0.0	5.295383310739611	0.0
6	1.3932408314303987E-4	0.0	0.0	6.319879735451431	0.0
7	1.3932408314303987E-4	0.0	0.0	7.516812933733286	0.0
8	1.3932408314303987E-4	0.0	0.0	9.317669591079172	0.0
9	1.3932408314303987E-4	0.0	0.0	9.958188842648774	0.0
10	1.3932408314303987E-4	0.0	0.0	12.071224328957165	0.0
11	1.857654441907198E-4	0.0	0.0	13.989995602003109	0.0
12	2.3220680523839977E-4	0.0	0.0	16.374155754107854	0.0
13	2.3220680523839977E-4	0.0	0.0	17.00724438790983	0.0
14	2.3220680523839977E-4	0.0	0.0	17.274189331211893	0.0
15	2.3220680523839977E-4	0.0	0.0	17.84291023860178	0.0
16	2.3220680523839977E-4	0.0	0.0	18.815485221662293	0.0
17	2.3220680523839977E-4	0.0	0.0	19.984878692842877	0.0
18	2.3220680523839977E-4	0.0	0.0	21.295175253442117	0.0
19	2.3220680523839977E-4	0.0	0.0	22.198784815346826	0.0
20	2.3220680523839977E-4	0.0	0.0	22.98118242491709	0.0
21	2.3220680523839977E-4	0.0	0.0	23.84434156134927	0.0
22	2.3220680523839977E-4	0.0	0.0	24.784825563925835	0.0
23	2.3220680523839977E-4	0.0	0.0	25.67877532273263	0.0
24	2.3220680523839977E-4	0.0	0.0	26.39016409126099	0.0
25	2.3220680523839977E-4	0.0	0.0	27.031287080524212	0.0
26	2.3220680523839977E-4	0.0	0.0	27.613708189423164	0.0
27	2.3220680523839977E-4	0.0	0.0	28.193203492576117	0.0
28	2.3220680523839977E-4	0.0	0.0	28.788117327596897	0.0
29	2.3220680523839977E-4	0.0	0.0	29.399935818039033	0.0
30	2.3220680523839977E-4	0.0	0.0	30.13900363775181	0.0
31	2.3220680523839977E-4	0.0	0.0	30.783331080927322	0.0
32	2.3220680523839977E-4	0.0	0.0	31.37127871179095	0.0
33	2.7864816628607973E-4	0.0	0.0	31.936470075741216	0.0
34	2.7864816628607973E-4	0.0	0.0	32.520795280443124	0.0
35	2.7864816628607973E-4	0.0	0.0	33.11120430344228	0.0
36	2.7864816628607973E-4	0.0	0.0	33.686752090906175	0.0
37	2.7864816628607973E-4	0.0	0.0	34.221849452897544	0.0
38	3.2508952733375964E-4	0.0	0.0	34.78982729851067	0.0
39	3.715308883814396E-4	0.0	0.0	35.355483076071415	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCAT	25	3.8919447E-5	45.000004	40
TCCGCGA	25	3.8919447E-5	45.000004	14
TAGCGAT	25	3.8919447E-5	45.000004	31
CGCTATA	25	3.8919447E-5	45.000004	21
ATCGTCC	20	7.0346624E-4	45.0	20
TACGCGC	20	7.0346624E-4	45.0	25
GTTAGCG	165	0.0	42.272728	1
AACGTAG	80	0.0	42.1875	1
GACCCGT	65	0.0	41.53846	20
TCGCAAG	60	3.6379788E-12	41.249996	1
AGTCGAA	95	0.0	40.263157	39
TAGGACG	175	0.0	39.857143	1
GACGTAT	85	0.0	39.705883	9
CGACGGT	290	0.0	39.568966	28
TCACGAC	325	0.0	38.76923	25
CACGCGA	35	6.2508334E-6	38.571426	11
CGCGATA	35	6.2508334E-6	38.571426	22
GATATCG	35	6.2508334E-6	38.571426	1
ATCCGGC	320	0.0	37.96875	6
GTCGAAA	30	1.1402531E-4	37.499996	40
>>END_MODULE