SRR2935038.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935038.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	493575
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	5392	1.0924378260649343	No Hit
GCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	2853	0.5780276553715241	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGC	2551	0.516841412146077	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTC	2354	0.47692853163146437	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCG	2309	0.46781137618396396	No Hit
CTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGCT	2253	0.45646558273818566	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1686	0.3415894240996809	No Hit
TCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	1439	0.2915463708656233	No Hit
ACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	1252	0.25365952489489946	No Hit
CGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG	1228	0.24879704198956595	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCC	1190	0.2410981107227878	No Hit
AAAACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTC	974	0.197335764574786	No Hit
ACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG	951	0.19267588512384135	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTC	900	0.18234310895000758	No Hit
AGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG	868	0.17585979840956287	No Hit
AGACATAGACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCC	808	0.16370359114622904	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	791	0.16025933242161777	No Hit
ACAGAAATAATAAATGGACCAAATCTCACGCTCAGTGTCTCCATCTGCTGC	747	0.1513447804285063	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCTCATT	680	0.13777034898445017	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	631	0.1278427797193942	No Hit
GCCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG	623	0.12622195208428305	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCT	579	0.11730740009117155	No Hit
GAGCCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTC	578	0.11710479663678267	No Hit
GGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG	575	0.11649698627361597	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT	562	0.11386314136656031	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTT	494	0.10008610646811528	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTAG	35	1.2100463E-7	45.000004	14
CCTTAGG	35	1.2100463E-7	45.000004	2
ATGATCG	70	0.0	45.000004	27
GATCGTG	85	0.0	45.000004	29
GTCGAGG	35	1.2100463E-7	45.000004	2
TTGTCGC	70	0.0	45.000004	38
TCCCTAT	35	1.2100463E-7	45.000004	23
TAAACGT	35	1.2100463E-7	45.000004	33
AAGCGCC	35	1.2100463E-7	45.000004	34
TTATCGG	35	1.2100463E-7	45.000004	13
ATCCGGC	35	1.2100463E-7	45.000004	6
GCCGATA	35	1.2100463E-7	45.000004	9
GTTGTCG	70	0.0	45.000004	37
GCCCTCA	70	0.0	45.000004	8
ACGTGAT	35	1.2100463E-7	45.000004	36
CATACAT	70	0.0	45.000004	33
TGCTCGA	35	1.2100463E-7	45.000004	17
CCAAGCG	140	0.0	45.000004	20
GCGGCTA	135	0.0	45.000004	24
CGACTTG	35	1.2100463E-7	45.000004	21

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content