##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935038.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	493575
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.050490806868254	33.0	31.0	34.0	30.0	34.0
2	32.244696348072736	34.0	31.0	34.0	30.0	34.0
3	32.247901534721166	34.0	31.0	34.0	30.0	34.0
4	35.832874436509144	37.0	35.0	37.0	35.0	37.0
5	35.717433014232896	37.0	35.0	37.0	35.0	37.0
6	35.6505434837664	37.0	35.0	37.0	33.0	37.0
7	35.93301524590994	37.0	35.0	37.0	35.0	37.0
8	35.5668642050347	37.0	35.0	37.0	35.0	37.0
9	37.25916831281973	39.0	37.0	39.0	34.0	39.0
10	37.23962923567847	39.0	37.0	39.0	34.0	39.0
11	37.162082763511116	39.0	37.0	39.0	34.0	39.0
12	36.83860001013017	39.0	35.0	39.0	33.0	39.0
13	36.577496834321025	39.0	35.0	39.0	33.0	39.0
14	37.574386871296156	39.0	36.0	41.0	33.0	41.0
15	37.70537608266221	39.0	36.0	41.0	33.0	41.0
16	37.851813807425415	40.0	36.0	41.0	33.0	41.0
17	37.859909841462795	39.0	36.0	41.0	34.0	41.0
18	37.855845616167755	39.0	36.0	41.0	34.0	41.0
19	37.761065694170085	39.0	36.0	41.0	34.0	41.0
20	37.672355771665906	39.0	35.0	41.0	33.0	41.0
21	37.60028567087069	39.0	35.0	41.0	33.0	41.0
22	37.48147900521704	39.0	35.0	41.0	33.0	41.0
23	37.23727700957301	39.0	35.0	41.0	33.0	41.0
24	36.971765182596364	39.0	35.0	41.0	32.0	41.0
25	36.964315453578486	39.0	35.0	40.0	32.0	41.0
26	37.18081142683483	39.0	35.0	41.0	33.0	41.0
27	37.23327153927975	39.0	35.0	41.0	33.0	41.0
28	37.19999392189637	39.0	35.0	41.0	33.0	41.0
29	37.294751557514054	39.0	35.0	41.0	33.0	41.0
30	37.20177683229499	39.0	35.0	41.0	33.0	41.0
31	37.03015144608216	39.0	35.0	41.0	33.0	41.0
32	36.88424049030036	39.0	35.0	41.0	32.0	41.0
33	36.81212784277972	39.0	35.0	41.0	32.0	41.0
34	36.71986425568556	39.0	35.0	41.0	32.0	41.0
35	36.591399483361194	39.0	35.0	41.0	31.0	41.0
36	36.535160816491924	39.0	35.0	41.0	31.0	41.0
37	36.4359236184977	39.0	35.0	41.0	31.0	41.0
38	36.27815225649597	39.0	35.0	40.0	31.0	41.0
39	36.31255027098212	39.0	35.0	40.0	31.0	41.0
40	36.18930658967735	39.0	35.0	40.0	30.0	41.0
41	36.11329787772882	38.0	35.0	40.0	30.0	41.0
42	36.11157372233197	38.0	35.0	40.0	30.0	41.0
43	36.042540647318035	38.0	35.0	40.0	30.0	41.0
44	35.911146229043204	38.0	35.0	40.0	30.0	41.0
45	35.931797599149064	38.0	35.0	40.0	30.0	41.0
46	35.791660841817354	38.0	35.0	40.0	30.0	41.0
47	35.70180823583042	38.0	35.0	40.0	30.0	41.0
48	35.68036063414881	38.0	35.0	40.0	30.0	41.0
49	35.687178240388995	38.0	35.0	40.0	30.0	41.0
50	35.61768728156815	38.0	35.0	40.0	30.0	41.0
51	34.63134680646305	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	4.0
11	8.0
12	4.0
13	11.0
14	14.0
15	31.0
16	51.0
17	120.0
18	200.0
19	390.0
20	638.0
21	1017.0
22	1719.0
23	2563.0
24	3294.0
25	3824.0
26	4350.0
27	4617.0
28	5161.0
29	6146.0
30	7599.0
31	9919.0
32	13024.0
33	18623.0
34	37111.0
35	56978.0
36	35036.0
37	54095.0
38	98413.0
39	128530.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.55670364179709	25.232842019956443	26.1222711847237	13.088183153522767
2	28.823988248999644	30.001722129362307	23.153725371017575	18.020564250620474
3	27.549207313984702	28.186395178037788	27.193030441169018	17.071367066808488
4	28.216785696196123	26.367826571443043	27.347414273413364	18.067973458947474
5	24.89104999240237	30.63647875196272	24.29762447449729	20.174846781137617
6	24.30370257812896	33.22392746796333	26.60831687180266	15.86405308210505
7	72.9015853720306	8.307552043762346	12.515625791419744	6.275236792787317
8	69.99301018082357	8.35111178645596	12.289520336321734	9.366357696398724
9	63.95603505039761	9.881983487818466	14.408144658866433	11.75383680291749
10	40.857417818973815	22.76837360076989	20.500835739249354	15.873372841006939
11	32.65015448513397	21.65587803272046	23.903358152256494	21.790609329889072
12	27.099022438332575	20.149723952793394	32.48017018690169	20.271083421972342
13	20.956288304715596	29.349136402775667	32.95385706326293	16.740718229245807
14	15.568657245606039	34.98110722787823	29.520336321734288	19.929899204781442
15	12.07334245048878	24.01316922453528	43.24125006331358	20.67223826166236
16	13.91845210960847	26.179202755406976	34.78944436002634	25.112900774958213
17	14.690979081193333	25.967279542116195	27.897887858987996	31.443853517702475
18	17.88016005672897	26.624525148153776	31.321886238160364	24.173428556956896
19	18.649445373043612	27.443853517702475	28.85741781897381	25.0492832902801
20	21.078660791166488	28.245960593628123	28.5729625690118	22.10241604619359
21	18.051562579141976	30.897026794306843	29.62974218710429	21.421668439446893
22	16.220229954920732	28.359823734994684	27.28339158182647	28.13655472825812
23	16.740718229245807	29.430380387985615	27.72243326748721	26.106468115281366
24	20.939674821455707	24.405206908777792	29.60644278984957	25.04867547991693
25	15.511928278377146	28.963379425619205	26.887301828496174	28.637390467507473
26	15.85675935774705	30.149420047611812	27.571696297421873	26.42212429721927
27	19.359975687585475	28.677100744567696	28.796839386111532	23.1660841817353
28	16.360634148812238	26.677404649749274	30.713265461176114	26.248695740262374
29	16.867547991693257	23.106316162690575	29.368586334397	30.657549511219166
30	20.054702932685004	25.990781542825303	29.390062300562224	24.56445322392747
31	24.13756774553006	26.28455655168921	22.29651015549815	27.281365547282583
32	20.59768019044725	28.184774350402677	22.573469077647772	28.644076381502302
33	21.049688497188875	24.476118117813908	28.507724256698573	25.966469128298638
34	18.65349744213139	23.260092184571747	27.04067264346857	31.045737729828293
35	16.52393253304969	24.911512941295648	28.209897178746896	30.354657346907764
36	20.926302993466038	22.223775515372537	33.325634402066555	23.52428708909487
37	20.884566681861926	23.249556804943524	31.65618193790204	24.20969457529251
38	19.7007546978676	26.837258775262118	26.59271640581472	26.869270121055568
39	19.872967634098163	22.139492478346757	31.388137567745527	26.59940231980955
40	20.182140505495617	23.273261409107025	30.378564554525656	26.1660335308717
41	16.864711543331815	25.58638504786507	27.13468064630502	30.4142227624981
42	20.857823025882592	21.93871245504736	30.904117915210456	26.299346603859597
43	24.001418224180725	20.111026693005115	31.413260396089754	24.47429468672441
44	20.394468925695183	21.558020564250622	29.519728511371117	28.52778199868308
45	19.947930912222052	21.303145418629388	26.83118067163045	31.917742997518104
46	24.07050600212734	21.36899154130578	29.80458896824191	24.75591348832498
47	17.305374056627663	22.107886339462087	34.32487463911259	26.26186496479765
48	18.6842931671985	20.160259332421617	32.07536848503267	29.08007901534721
49	19.40353543027909	17.42025021526617	37.088993567340324	26.08722078711442
50	19.06822671326546	18.265309223522262	34.01671478498709	28.649749278225194
51	17.075824342805046	20.350503976092792	28.084688243934558	34.488983437167605
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1972.0
1	1793.0
2	1614.0
3	1713.5
4	1813.0
5	1520.0
6	1227.0
7	1280.5
8	1334.0
9	1467.5
10	1601.0
11	1801.0
12	2001.0
13	1934.0
14	1867.0
15	1936.5
16	2006.0
17	1948.5
18	1891.0
19	2035.0
20	2179.0
21	2060.5
22	1942.0
23	1950.5
24	1959.0
25	1773.0
26	1828.5
27	2070.0
28	2774.0
29	3478.0
30	3960.5
31	4443.0
32	4271.0
33	4099.0
34	5398.5
35	6698.0
36	7791.0
37	8884.0
38	10015.0
39	11146.0
40	14203.5
41	17261.0
42	23318.0
43	29375.0
44	33804.0
45	38233.0
46	51415.5
47	64598.0
48	68662.0
49	72726.0
50	70887.5
51	69049.0
52	55070.5
53	41092.0
54	34167.0
55	27242.0
56	22175.0
57	17108.0
58	15789.0
59	14470.0
60	13039.5
61	11609.0
62	10265.5
63	8922.0
64	7374.0
65	5826.0
66	4998.5
67	4171.0
68	3295.0
69	2419.0
70	1875.0
71	1331.0
72	1118.0
73	905.0
74	798.5
75	486.5
76	281.0
77	236.0
78	191.0
79	170.5
80	150.0
81	119.0
82	88.0
83	53.0
84	18.0
85	10.5
86	3.0
87	3.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	493575.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.438814916167463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.36520327147525	18.07494956000701
2	12.165945616178805	6.433063688526208
3	5.702532306605693	4.523045886234405
4	3.1879525412302634	3.3714274879645068
5	2.0103380732132523	2.6575478118304954
6	1.337812311182619	2.12221032527565
7	1.0092039116266638	1.8677508802518714
8	0.7052113881226334	1.4915962693878275
9	0.5559786948238373	1.322947602880181
>10	3.6210559548789836	20.627737826292346
>50	1.0342562761288747	19.150305903224123
>100	0.28528556018127327	11.2876918525708
>500	0.010765492837029178	2.0873315006996345
>1k	0.007689637740735128	3.8861733355287336
>5k	7.689637740735129E-4	1.0962200693262325
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	5392	1.0924378260649343	No Hit
GCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	2853	0.5780276553715241	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGC	2551	0.516841412146077	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTC	2354	0.47692853163146437	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCG	2309	0.46781137618396396	No Hit
CTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGCT	2253	0.45646558273818566	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1686	0.3415894240996809	No Hit
TCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	1439	0.2915463708656233	No Hit
ACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	1252	0.25365952489489946	No Hit
CGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG	1228	0.24879704198956595	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCC	1190	0.2410981107227878	No Hit
AAAACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTC	974	0.197335764574786	No Hit
ACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG	951	0.19267588512384135	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTC	900	0.18234310895000758	No Hit
AGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG	868	0.17585979840956287	No Hit
AGACATAGACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCC	808	0.16370359114622904	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	791	0.16025933242161777	No Hit
ACAGAAATAATAAATGGACCAAATCTCACGCTCAGTGTCTCCATCTGCTGC	747	0.1513447804285063	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCTCATT	680	0.13777034898445017	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	631	0.1278427797193942	No Hit
GCCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG	623	0.12622195208428305	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCT	579	0.11730740009117155	No Hit
GAGCCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTC	578	0.11710479663678267	No Hit
GGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG	575	0.11649698627361597	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT	562	0.11386314136656031	No Hit
AAAAACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTT	494	0.10008610646811528	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.052069087777947E-4	0.0	0.0	0.48381704908068685	0.0
2	4.052069087777947E-4	0.0	0.0	2.80382920528795	0.0
3	4.052069087777947E-4	0.0	0.0	4.094210606290837	0.0
4	4.052069087777947E-4	0.0	0.0	5.641290584004457	0.0
5	4.052069087777947E-4	0.0	0.0	9.819986830775465	0.0
6	4.052069087777947E-4	0.0	0.0	11.813604821962214	0.0
7	4.052069087777947E-4	0.0	0.0	14.004963784632528	0.0
8	4.052069087777947E-4	0.0	0.0	17.00329230613382	0.0
9	4.052069087777947E-4	0.0	0.0	18.062705769133363	0.0
10	4.052069087777947E-4	0.0	0.0	20.59707238008408	0.0
11	4.052069087777947E-4	0.0	0.0	24.194296712758952	0.0
12	4.052069087777947E-4	0.0	0.0	27.372942308666364	0.0
13	4.052069087777947E-4	0.0	0.0	28.365091424808792	0.0
14	4.052069087777947E-4	0.0	0.0	28.709719900724306	0.0
15	4.052069087777947E-4	0.0	0.0	29.482044268854786	0.0
16	4.052069087777947E-4	0.0	0.0	30.94139695081801	0.0
17	4.052069087777947E-4	0.0	0.0	32.645089398774246	0.0
18	4.052069087777947E-4	0.0	0.0	34.786810515119285	0.0
19	4.052069087777947E-4	0.0	0.0	36.030187914703944	0.0
20	4.052069087777947E-4	0.0	0.0	37.06528896317682	0.0
21	6.07810363166692E-4	0.0	0.0	38.23127184318493	0.0
22	6.07810363166692E-4	0.0	0.0	39.32593830724814	0.0
23	6.07810363166692E-4	0.0	0.0	40.27067821506357	0.0
24	6.07810363166692E-4	0.0	0.0	41.0018740819531	0.0
25	6.07810363166692E-4	0.0	0.0	41.684445119789295	0.0
26	6.07810363166692E-4	0.0	0.0	42.335410018740816	0.0
27	6.07810363166692E-4	0.0	0.0	42.92377045028618	0.0
28	6.07810363166692E-4	0.0	0.0	43.486805450032925	0.0
29	6.07810363166692E-4	0.0	0.0	44.06868257103783	0.0
30	6.07810363166692E-4	0.0	0.0	44.76320721268298	0.0
31	6.07810363166692E-4	0.0	0.0	45.42531530162589	0.0
32	6.07810363166692E-4	0.0	0.0	46.047510510054195	0.0
33	6.07810363166692E-4	0.0	0.0	46.58339664691283	0.0
34	8.104138175555894E-4	0.0	0.0	47.17661956136352	0.0
35	8.104138175555894E-4	0.0	0.0	47.8192777186851	0.0
36	8.104138175555894E-4	0.0	0.0	48.33996859646457	0.0
37	8.104138175555894E-4	0.0	0.0	48.849921491161425	0.0
38	8.104138175555894E-4	0.0	0.0	49.362103023856555	0.0
39	8.104138175555894E-4	0.0	0.0	49.87813402218508	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTAG	35	1.2100463E-7	45.000004	14
CCTTAGG	35	1.2100463E-7	45.000004	2
ATGATCG	70	0.0	45.000004	27
GATCGTG	85	0.0	45.000004	29
GTCGAGG	35	1.2100463E-7	45.000004	2
TTGTCGC	70	0.0	45.000004	38
TCCCTAT	35	1.2100463E-7	45.000004	23
TAAACGT	35	1.2100463E-7	45.000004	33
AAGCGCC	35	1.2100463E-7	45.000004	34
TTATCGG	35	1.2100463E-7	45.000004	13
ATCCGGC	35	1.2100463E-7	45.000004	6
GCCGATA	35	1.2100463E-7	45.000004	9
GTTGTCG	70	0.0	45.000004	37
GCCCTCA	70	0.0	45.000004	8
ACGTGAT	35	1.2100463E-7	45.000004	36
CATACAT	70	0.0	45.000004	33
TGCTCGA	35	1.2100463E-7	45.000004	17
CCAAGCG	140	0.0	45.000004	20
GCGGCTA	135	0.0	45.000004	24
CGACTTG	35	1.2100463E-7	45.000004	21
>>END_MODULE