FastQCFastQC Report
Sat 14 Jan 2017
SRR2935037.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935037.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3819509
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111020.29066563267687023No Hit
GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC39090.10234299748999151TruSeq Adapter, Index 27 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTCG351.2129203E-745.042
CGTTTTT62550.040.899281
CCGTAAA403.4610457E-739.37543
TAATACG356.252103E-638.5714261
TATTCGC356.252103E-638.57142622
ACGTTAG1000.038.251
CGAATCG301.14041846E-437.50000424
ACAACGA11200.037.3660713
AATTACG552.752131E-936.818181
ATTAGCG1900.036.710531
CACAACG11450.036.5502212
ACGGGTA2350.036.382985
CGACTAT250.002107720136.027
TACGGGT3200.035.8593754
TACGAAT3050.035.40983612
AACGAGC12150.035.18518415
AGCGACT11800.034.7033919
TACACGC29000.034.6034535
GGCGATA12500.034.5599988
TTACACG29000.034.29310634
Produced by FastQC (version 0.11.5)