##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935037.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3819509 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45524568733835 33.0 31.0 34.0 31.0 34.0 2 32.6374170083118 34.0 31.0 34.0 31.0 34.0 3 32.62316753279021 34.0 31.0 34.0 31.0 34.0 4 36.1126794046041 37.0 35.0 37.0 35.0 37.0 5 36.043653516721655 37.0 35.0 37.0 35.0 37.0 6 35.990253197465954 37.0 35.0 37.0 35.0 37.0 7 36.20304599360808 37.0 36.0 37.0 35.0 37.0 8 35.968241205872275 37.0 37.0 37.0 35.0 37.0 9 37.712142581677384 39.0 38.0 39.0 35.0 39.0 10 37.55177118315469 39.0 37.0 39.0 35.0 39.0 11 37.45357950459077 39.0 37.0 39.0 35.0 39.0 12 37.20742770864004 39.0 37.0 39.0 34.0 39.0 13 37.12557216123852 39.0 37.0 39.0 34.0 39.0 14 38.155403482489504 40.0 37.0 41.0 33.0 41.0 15 38.18706069287963 40.0 37.0 41.0 33.0 41.0 16 38.265284621662104 40.0 37.0 41.0 34.0 41.0 17 38.27202737315189 40.0 37.0 41.0 34.0 41.0 18 38.27260362523036 40.0 37.0 41.0 34.0 41.0 19 38.26692148126893 40.0 37.0 41.0 34.0 41.0 20 38.242233229454364 40.0 37.0 41.0 34.0 41.0 21 38.20227914111474 40.0 36.0 41.0 34.0 41.0 22 38.14316787838437 40.0 36.0 41.0 34.0 41.0 23 37.95016820224799 40.0 36.0 41.0 34.0 41.0 24 37.78380441046218 40.0 35.0 41.0 33.0 41.0 25 37.74945339832947 40.0 35.0 41.0 33.0 41.0 26 37.885450721545624 40.0 36.0 41.0 34.0 41.0 27 37.928299946406725 40.0 36.0 41.0 34.0 41.0 28 37.8932949235098 40.0 36.0 41.0 34.0 41.0 29 37.90957843010712 40.0 36.0 41.0 34.0 41.0 30 37.821384895283664 40.0 36.0 41.0 34.0 41.0 31 37.71824493671831 40.0 35.0 41.0 33.0 41.0 32 37.626456175387986 40.0 35.0 41.0 33.0 41.0 33 37.539740841034806 40.0 35.0 41.0 33.0 41.0 34 37.369232275666846 40.0 35.0 41.0 33.0 41.0 35 37.28682665756253 40.0 35.0 41.0 33.0 41.0 36 37.23868853300254 40.0 35.0 41.0 33.0 41.0 37 37.203815726052746 40.0 35.0 41.0 33.0 41.0 38 37.15091390018979 40.0 35.0 41.0 32.0 41.0 39 37.08201970462696 39.0 35.0 41.0 32.0 41.0 40 37.03219759398394 39.0 35.0 41.0 32.0 41.0 41 36.95067821544602 39.0 35.0 41.0 32.0 41.0 42 36.924264349161106 39.0 35.0 41.0 32.0 41.0 43 36.8380605988885 39.0 35.0 41.0 32.0 41.0 44 36.70257381249789 39.0 35.0 41.0 32.0 41.0 45 36.681499114153155 39.0 35.0 41.0 32.0 41.0 46 36.57899065036894 39.0 35.0 41.0 31.0 41.0 47 36.5007447292309 39.0 35.0 41.0 31.0 41.0 48 36.41867501817642 38.0 35.0 41.0 31.0 41.0 49 36.349231537352054 38.0 35.0 41.0 31.0 41.0 50 36.28161001846049 38.0 35.0 40.0 31.0 41.0 51 35.381938097279 37.0 34.0 40.0 29.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 4.0 8 26.0 9 54.0 10 72.0 11 71.0 12 69.0 13 83.0 14 112.0 15 175.0 16 279.0 17 576.0 18 960.0 19 1551.0 20 2605.0 21 4196.0 22 6326.0 23 9378.0 24 13416.0 25 18916.0 26 25288.0 27 30542.0 28 35013.0 29 40218.0 30 48808.0 31 61595.0 32 82310.0 33 122881.0 34 259990.0 35 318527.0 36 255749.0 37 403431.0 38 748348.0 39 1327005.0 40 935.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.48048872250334 21.616783727960843 25.104692775956284 11.798034773579536 2 31.708997151204514 23.420628148801327 26.637271963490594 18.233102736503568 3 32.12865842180238 23.94210878937581 26.944719857971272 16.98451293085054 4 28.46399890666575 25.51330550602185 26.379621045532293 19.64307454178011 5 25.764280173184563 29.649125057697205 25.456753734576882 19.12984103454135 6 27.66701164992673 33.427909189374866 23.59083327202528 15.314245888673126 7 82.9729423336874 5.647558364177176 7.161862951494551 4.2176363506408805 8 84.7726239157965 4.215777472968385 6.957124593763229 4.054474017471879 9 79.09702529827787 6.569038062222134 8.76322061291124 5.570716026588758 10 45.04806769665944 27.429049126471494 14.326867668069376 13.196015508799691 11 35.93027271306338 24.16022583007397 21.937322310276006 17.97217914658664 12 32.27181818396029 22.53124681732652 25.570668900112555 19.62626609860063 13 28.5833336169649 22.982168650473138 28.103245731323057 20.3312520012389 14 23.354965258623555 24.26838109296247 31.23087286873784 21.145780779676134 15 23.462727800877023 23.383136418843364 33.24309485852763 19.911040921751987 16 27.774224383291152 23.977270376899227 30.042500227123437 18.206005012686184 17 27.88879408321855 23.418455094620803 28.495311831965836 20.19743899019481 18 27.928773043865064 24.647461231273443 28.414149567392037 19.009616157469456 19 25.936029997572984 28.925419471455626 25.728490232645086 19.410060298326304 20 26.76467053749579 29.87891899194373 25.65866450373595 17.69774596682453 21 27.247481286207204 28.084002420206367 26.40962490204893 18.2588913915375 22 25.651621713681 24.557999470612586 28.70366321953948 21.08671559616694 23 23.098623409448702 25.41975945075663 31.08917926361739 20.392437876177276 24 23.702627746131768 23.231808067476738 31.737115948672983 21.328448237718515 25 24.15161215747888 23.934516190431808 29.088162902613924 22.82570874947539 26 22.007043313682466 24.56190049558726 28.715994647479558 24.715061543250716 27 20.810894803494374 25.536214209732194 28.72714791351454 24.925743073258893 28 19.332563426346162 27.11662677061371 28.978201124804258 24.57260867823587 29 21.91559176847076 25.231620085199435 27.6047785199616 25.248009626368205 30 23.465738659078955 23.465476845322264 26.05596687951252 27.012817616086256 31 23.28647477987354 26.568362582729875 25.45924096526543 24.685921672131155 32 24.125090423926217 27.333722737660782 25.199128997994247 23.342057840418757 33 23.509356830943453 25.69492570903747 26.79684744819295 23.998870011826128 34 23.014895370059346 24.656284354873886 29.823990465790235 22.504829809276533 35 22.599030398933476 25.850888163897505 27.097043101613323 24.453038335555696 36 22.09891376090487 29.447031018908454 25.496915964853073 22.957139255333605 37 22.538970323148867 28.534138812082915 25.67183373569744 23.255057129070778 38 22.58392374517248 25.84897692347367 27.122674668393245 24.444424662960607 39 22.61005275809011 23.604683219754165 28.014909769815965 25.770354252339768 40 22.809188301428275 22.760674212313678 29.97869621461816 24.45144127163989 41 20.4894398730308 23.32794607893318 31.08420480224029 25.09840924579573 42 23.398583430488053 23.608688970231515 29.203858401695086 23.788869197585345 43 23.433849743514156 22.487131199324313 29.35002902205493 24.72899003510661 44 21.9053548505842 22.401334831257106 30.82739168830339 24.865918629855305 45 22.37211641601054 21.781307492664634 31.014326710579816 24.83224938074501 46 23.765332140858945 23.14855129284942 29.071668635942473 24.01444793034916 47 23.903857799523447 23.883226875496298 29.276537900552142 22.936377424428116 48 22.774393253164217 23.170334197406 29.04899556461315 25.006276984816633 49 22.97397387988875 21.985286590501556 30.328269942550207 24.712469587059488 50 20.889229479495924 22.566958213738992 31.55761643708655 24.986195869678536 51 20.301981223240997 22.201518572151553 31.212100822383192 26.28439938222426 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 930.0 1 1975.5 2 3021.0 3 11797.0 4 20573.0 5 13667.5 6 6762.0 7 6626.5 8 6491.0 9 6595.0 10 6699.0 11 6720.5 12 6742.0 13 6830.5 14 6919.0 15 7016.5 16 7114.0 17 7130.0 18 7146.0 19 7427.0 20 7708.0 21 8570.5 22 9433.0 23 10106.5 24 10780.0 25 13947.5 26 21128.5 27 25142.0 28 31719.5 29 38297.0 30 42806.5 31 47316.0 32 55086.0 33 62856.0 34 74274.0 35 85692.0 36 95639.0 37 105586.0 38 118923.5 39 132261.0 40 155792.0 41 179323.0 42 203367.5 43 227412.0 44 242550.0 45 257688.0 46 277624.5 47 297561.0 48 310975.5 49 324390.0 50 324474.0 51 324558.0 52 296203.5 53 267849.0 54 251646.0 55 235443.0 56 222837.0 57 210231.0 58 205376.0 59 200521.0 60 193166.0 61 185811.0 62 173310.5 63 160810.0 64 137436.0 65 114062.0 66 96268.0 67 78474.0 68 66607.5 69 54741.0 70 45293.5 71 35846.0 72 29354.0 73 22862.0 74 17941.5 75 10410.0 76 7799.0 77 5535.0 78 3271.0 79 2359.5 80 1448.0 81 1221.5 82 995.0 83 692.5 84 390.0 85 329.0 86 268.0 87 176.5 88 85.0 89 66.5 90 48.0 91 30.5 92 13.0 93 9.0 94 5.0 95 3.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 3819509.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.416061178892676 #Duplication Level Percentage of deduplicated Percentage of total 1 71.92918939972489 13.965815419332166 2 10.786726069036776 4.18871466552749 3 4.070469090153442 2.3709743064363247 4 2.0981460167183954 1.6295092569141734 5 1.219191969629387 1.1835952935569416 6 0.8234328629019069 0.95926937056885 7 0.5820703789107198 0.7911059861146242 8 0.4399725066555107 0.6834026485003328 9 0.3806496484915272 0.6651645176551935 >10 4.680821436180891 23.153429866780673 >50 2.382194385376022 33.29528193059191 >100 0.5988457342602991 14.830741752989985 >500 0.0038689009148133017 0.5110494079681005 >1k 0.004283426012828952 1.4740994682248387 >5k 0.0 0.0 >10k+ 1.3817503267190168E-4 0.29784610883835333 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11102 0.29066563267687023 No Hit GCTGTCTCTTATACACATCTGACGCCCACTTTGTCGTATGCCGTCTTCTGC 3909 0.10234299748999151 TruSeq Adapter, Index 27 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.3563238102070188E-4 0.0 0.0 0.07676379346141088 0.0 2 2.3563238102070188E-4 0.0 0.0 0.3450181685656455 0.0 3 2.3563238102070188E-4 0.0 0.0 0.5820381624968026 0.0 4 2.3563238102070188E-4 0.0 0.0 0.8578327737936997 0.0 5 2.3563238102070188E-4 0.0 0.0 1.69055760832086 0.0 6 2.3563238102070188E-4 0.0 0.0 2.4088960125503043 0.0 7 2.618137566896687E-4 0.0 0.0 3.0544240110443517 0.0 8 2.618137566896687E-4 0.0 0.0 3.9347465865376936 0.0 9 2.618137566896687E-4 0.0 0.0 4.375928947935455 0.0 10 2.618137566896687E-4 0.0 0.0 5.196819800660242 0.0 11 2.618137566896687E-4 0.0 0.0 6.290206411347636 0.0 12 2.618137566896687E-4 0.0 0.0 7.043470770719482 0.0 13 2.8799513235863564E-4 0.0 0.0 7.3868133312423145 0.0 14 3.403578836965694E-4 0.0 0.0 7.547278982717412 0.0 15 3.6653925936553625E-4 0.0 0.0 7.78552950130501 0.0 16 3.927206350345031E-4 0.0 0.0 8.181522808298135 0.0 17 3.927206350345031E-4 0.0 0.0 8.646765853935676 0.0 18 3.927206350345031E-4 0.0 0.0 9.131618749949274 0.0 19 4.450833863724369E-4 0.0 0.0 9.462629882532022 0.0 20 4.7126476204140377E-4 0.0 0.0 9.818853679883984 0.0 21 4.7126476204140377E-4 0.0 0.0 10.255977928053056 0.0 22 4.974461377103706E-4 0.0 0.0 10.722765674855067 0.0 23 5.236275133793374E-4 0.0 0.0 11.214478091293934 0.0 24 5.236275133793374E-4 0.0 0.0 11.599370495003415 0.0 25 5.498088890483044E-4 0.0 0.0 11.9327117700207 0.0 26 5.498088890483044E-4 0.0 0.0 12.244846130746124 0.0 27 5.498088890483044E-4 0.0 0.0 12.586172725342445 0.0 28 5.498088890483044E-4 0.0 0.0 12.894327516966186 0.0 29 5.498088890483044E-4 0.0 0.0 13.230627287434066 0.0 30 5.498088890483044E-4 0.0 0.0 13.631228516544928 0.0 31 5.759902647172713E-4 0.0 0.0 13.981954225006408 0.0 32 5.759902647172713E-4 0.0 0.0 14.324406618756495 0.0 33 6.021716403862382E-4 0.0 0.0 14.666099752612181 0.0 34 6.021716403862382E-4 0.0 0.0 15.020229039910626 0.0 35 6.021716403862382E-4 0.0 0.0 15.39574851113062 0.0 36 6.28353016055205E-4 0.0 0.0 15.767262231873259 0.0 37 6.545343917241719E-4 0.0 0.0 16.149641223518522 0.0 38 6.807157673931388E-4 0.0 0.0 16.595064967774654 0.0 39 6.807157673931388E-4 0.0 0.0 17.2091229527146 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATTCG 35 1.2129203E-7 45.0 42 CGTTTTT 6255 0.0 40.89928 1 CCGTAAA 40 3.4610457E-7 39.375 43 TAATACG 35 6.252103E-6 38.571426 1 TATTCGC 35 6.252103E-6 38.571426 22 ACGTTAG 100 0.0 38.25 1 CGAATCG 30 1.14041846E-4 37.500004 24 ACAACGA 1120 0.0 37.36607 13 AATTACG 55 2.752131E-9 36.81818 1 ATTAGCG 190 0.0 36.71053 1 CACAACG 1145 0.0 36.55022 12 ACGGGTA 235 0.0 36.38298 5 CGACTAT 25 0.0021077201 36.0 27 TACGGGT 320 0.0 35.859375 4 TACGAAT 305 0.0 35.409836 12 AACGAGC 1215 0.0 35.185184 15 AGCGACT 1180 0.0 34.70339 19 TACACGC 2900 0.0 34.60345 35 GGCGATA 1250 0.0 34.559998 8 TTACACG 2900 0.0 34.293106 34 >>END_MODULE