##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935036.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1712852
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.23579270129585	33.0	31.0	34.0	30.0	34.0
2	32.410125918643296	34.0	31.0	34.0	30.0	34.0
3	32.41104251855969	34.0	31.0	34.0	30.0	34.0
4	35.958543411806744	37.0	35.0	37.0	35.0	37.0
5	35.872301284641054	37.0	35.0	37.0	35.0	37.0
6	35.81023579386894	37.0	35.0	37.0	35.0	37.0
7	36.12947995506909	37.0	35.0	37.0	35.0	37.0
8	35.910520582046786	37.0	35.0	37.0	35.0	37.0
9	37.64463421241298	39.0	38.0	39.0	35.0	39.0
10	37.434010644235464	39.0	37.0	39.0	35.0	39.0
11	37.3480446646879	39.0	37.0	39.0	34.0	39.0
12	37.217170543631326	39.0	37.0	39.0	34.0	39.0
13	37.137082479980755	39.0	37.0	39.0	33.0	39.0
14	38.20594423803107	40.0	38.0	41.0	33.0	41.0
15	38.303244530175405	40.0	38.0	41.0	34.0	41.0
16	38.370564415372726	40.0	37.0	41.0	34.0	41.0
17	38.41604411823088	40.0	37.0	41.0	34.0	41.0
18	38.413343943317926	40.0	37.0	41.0	34.0	41.0
19	38.39677567005205	40.0	37.0	41.0	34.0	41.0
20	38.35938072874948	40.0	37.0	41.0	34.0	41.0
21	38.31077758031634	40.0	37.0	41.0	34.0	41.0
22	38.23561405188539	40.0	37.0	41.0	34.0	41.0
23	37.99875529234283	40.0	36.0	41.0	34.0	41.0
24	37.795326741598224	40.0	36.0	41.0	33.0	41.0
25	37.7598187117159	39.0	36.0	41.0	33.0	41.0
26	37.93006284255733	40.0	36.0	41.0	34.0	41.0
27	38.00155413310665	40.0	37.0	41.0	34.0	41.0
28	37.996064458575525	40.0	37.0	41.0	34.0	41.0
29	38.03305130857774	40.0	37.0	41.0	34.0	41.0
30	37.96571682784035	40.0	36.0	41.0	34.0	41.0
31	37.8651185274618	40.0	36.0	41.0	34.0	41.0
32	37.7962182371857	40.0	36.0	41.0	34.0	41.0
33	37.7320305548874	40.0	36.0	41.0	33.0	41.0
34	37.56435173616868	40.0	36.0	41.0	33.0	41.0
35	37.51371630473619	40.0	36.0	41.0	33.0	41.0
36	37.44656164105247	40.0	36.0	41.0	33.0	41.0
37	37.295262521221915	39.0	35.0	41.0	33.0	41.0
38	37.22779901591031	39.0	35.0	41.0	33.0	41.0
39	37.15312998437693	39.0	35.0	41.0	32.0	41.0
40	37.05040365425618	39.0	35.0	41.0	32.0	41.0
41	37.039996450364654	39.0	35.0	41.0	32.0	41.0
42	37.032391590166576	39.0	35.0	41.0	32.0	41.0
43	36.92240018402057	39.0	35.0	41.0	32.0	41.0
44	36.79800064453905	39.0	35.0	41.0	31.0	41.0
45	36.83118973501505	39.0	35.0	41.0	32.0	41.0
46	36.763294785538974	39.0	35.0	41.0	31.0	41.0
47	36.710251089994934	39.0	35.0	41.0	31.0	41.0
48	36.635769465196056	39.0	35.0	40.0	31.0	41.0
49	36.568946412182726	39.0	35.0	40.0	31.0	41.0
50	36.46694810760066	39.0	35.0	40.0	31.0	41.0
51	35.38182283116113	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	23.0
10	24.0
11	25.0
12	20.0
13	20.0
14	29.0
15	39.0
16	87.0
17	150.0
18	273.0
19	555.0
20	947.0
21	1602.0
22	2479.0
23	3694.0
24	5244.0
25	7102.0
26	9089.0
27	10967.0
28	13138.0
29	16346.0
30	20985.0
31	27930.0
32	38082.0
33	55061.0
34	103520.0
35	136713.0
36	129070.0
37	208826.0
38	389008.0
39	531480.0
40	322.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.577822252010094	24.478005104936095	27.401900456081435	9.54227218697237
2	30.51232680932153	25.571211056179987	26.315933892712273	17.600528241786215
3	31.130477122366674	24.856963707313884	27.831126098460345	16.181433071859097
4	28.907809898344983	26.704175258574587	26.529670981497528	17.858343861582902
5	25.20713990467361	29.549663368463825	25.572028406423907	19.67116832043866
6	26.61858701160404	34.041119723128446	24.112240870781598	15.228052394485921
7	80.89694848124648	5.682627570858427	8.281509435724745	5.138914512170345
8	80.87161062368494	4.472190241772203	8.366455478932213	6.289743655610643
9	75.17135164042193	7.5878709894375	10.17892964482629	7.061847725314271
10	39.99919432618814	31.602730416871978	15.404016225569986	12.994059031369904
11	29.903984699203434	25.43856678802372	24.667747125846248	19.9897013869266
12	27.57628796883794	23.160611658216823	29.516502301424758	19.74659807152048
13	25.585514685448597	25.990862024272964	30.69033401601539	17.733289274263043
14	20.755675329800823	29.99243367202771	28.272378465856946	20.979512532314526
15	18.47941328264205	27.074318154750088	34.489086038957254	19.957182523650612
16	21.77269256187925	27.630583377898382	30.061499767639	20.535224292583365
17	22.23758970418927	26.272497565463915	26.54911224087078	24.940800489476032
18	22.80267063353985	26.69425029132698	29.187869121208372	21.3152099539248
19	23.68278169976157	27.698948887586315	26.21142982581099	22.40683958684113
20	24.359430937407318	29.30959592539227	26.592665332439697	19.738307804760716
21	23.810988923736552	27.607347278106925	28.672996849698627	19.908666948457892
22	22.013168680072766	26.59564282261398	25.785473584407757	25.60571491290549
23	20.706459168684745	28.202495020001727	27.20118258903863	23.889863222274897
24	23.05202084009593	26.30933670860063	27.385203158241346	23.253439293062097
25	20.92620961997884	29.10484968929014	25.72066938649691	24.24827130423411
26	20.685441590983928	28.929236151167757	27.61178432228821	22.773537935560107
27	21.93190071296294	27.938549273375635	28.459785200355896	21.669764813305527
28	19.62271112740622	26.964501311263323	29.221438863369396	24.19134869796106
29	20.438309906518484	24.872259833307258	30.885505577831594	23.803924682342668
30	23.078584723023354	25.57897588349723	28.78403971855128	22.558399674928133
31	24.197011767508226	27.944037196441958	25.34684841422376	22.512102621826056
32	24.292700128207223	27.454911457615722	26.945468727012024	21.30691968716503
33	24.525236272602655	26.12823524741192	25.20953357324509	24.136994906740338
34	22.450918117852563	27.105202317538236	27.487897378173948	22.955982186435257
35	22.928717717584473	25.044895881255357	28.845399369005616	23.180987032154558
36	26.40905343835895	25.389175480426797	27.079630931335576	21.122140149878682
37	24.317862839287923	26.902732985687027	28.955099448171822	19.824304726853224
38	24.738564686265946	26.31050435180623	25.899493943434692	23.051437018493136
39	24.572876115391175	24.798464782713275	26.45978753564231	24.168871566253245
40	24.482967588559898	25.139066305787072	28.097348749337364	22.280617356315666
41	24.061506773498238	26.37647619292268	25.609509753323696	23.952507280255386
42	26.07253866650475	23.921272824505564	28.156665024181894	21.849523484807793
43	25.865515526151704	22.976882999815512	29.183957516469604	21.973643957563173
44	23.054356126507138	24.258721710924235	27.395828711412314	25.291093451156314
45	22.94722486239325	23.040110879398803	27.080973721022016	26.931690537185933
46	24.466678965841766	23.995126257259823	28.026356042436824	23.511838734461588
47	21.73258401776686	24.687655442501747	30.702827798315326	22.87693274141607
48	23.014072435913903	23.282805519682963	29.449829874384942	24.25329217001819
49	24.74370231637059	21.287186516990374	30.76202730883929	23.20708385779974
50	21.677529640622776	22.56861655297714	29.515568186860275	26.23828561953981
51	21.947138456796033	22.239808226279912	26.26771022832095	29.545343088603104
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	834.0
1	1167.0
2	1500.0
3	2345.0
4	3190.0
5	2441.5
6	1693.0
7	1819.0
8	1945.0
9	2234.0
10	2523.0
11	2764.0
12	3005.0
13	3144.0
14	3283.0
15	3443.5
16	3604.0
17	3701.0
18	3798.0
19	3740.0
20	3682.0
21	3598.0
22	3514.0
23	3887.0
24	4260.0
25	4769.0
26	7406.0
27	9534.0
28	10996.5
29	12459.0
30	15089.5
31	17720.0
32	20037.0
33	22354.0
34	26845.5
35	31337.0
36	36684.5
37	42032.0
38	49351.5
39	56671.0
40	63589.5
41	70508.0
42	83933.5
43	97359.0
44	113685.0
45	130011.0
46	159991.5
47	189972.0
48	213892.5
49	237813.0
50	229868.0
51	221923.0
52	182067.5
53	142212.0
54	119334.0
55	96456.0
56	84755.0
57	73054.0
58	66746.5
59	60439.0
60	54496.0
61	48553.0
62	44154.5
63	39756.0
64	32925.0
65	26094.0
66	22083.0
67	18072.0
68	13537.5
69	9003.0
70	7785.5
71	6568.0
72	5685.5
73	4803.0
74	3864.5
75	2283.5
76	1641.0
77	1264.0
78	887.0
79	593.5
80	300.0
81	222.5
82	145.0
83	130.5
84	116.0
85	63.0
86	10.0
87	8.0
88	6.0
89	3.5
90	1.0
91	1.0
92	1.0
93	2.0
94	3.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1712852.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.833786623060018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.31211517640679	16.219471321916064
2	13.89844413182384	6.903019919244676
3	6.205015755130505	4.622821117669098
4	3.3397451158068496	3.3175407032541666
5	2.114043229183578	2.6249849232734865
6	1.3837609980817018	2.0618415218204125
7	0.9849487417647179	1.7122004821345511
8	0.7314770251171205	1.4532275489143427
9	0.5451048273938147	1.218331527362816
>10	4.031234378598889	21.166311274663485
>50	0.953694273051178	17.048764284036917
>100	0.48662815567847084	17.015095050484405
>500	0.00903364300999335	1.5214354938165362
>1k	0.004041366609733868	1.7982587071379337
>5k	7.131823428942119E-4	1.316696124271088
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	8720	0.5090924376420146	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	7836	0.4574826079544526	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT	5747	0.3355222751294332	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	3887	0.2269314570085448	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	2828	0.16510474927197447	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCC	2570	0.1500421519197222	No Hit
ACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTG	2058	0.12015048585633785	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTG	1955	0.11413712334749294	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.34947561143636463	0.0
2	0.0	0.0	0.0	1.7641921193424768	0.0
3	0.0	0.0	0.0	2.5512420220777976	0.0
4	0.0	0.0	0.0	3.514197373736902	0.0
5	0.0	0.0	0.0	6.070109968637103	0.0
6	0.0	0.0	0.0	7.358662628178033	0.0
7	0.0	0.0	0.0	8.73175265580447	0.0
8	0.0	0.0	0.0	10.798597894038714	0.0
9	0.0	0.0	0.0	11.50578100151093	0.0
10	0.0	0.0	0.0	13.304301831098075	0.0
11	0.0	0.0	0.0	16.107638021265117	0.0
12	5.8382160280047546E-5	0.0	0.0	18.263866346888115	0.0
13	5.8382160280047546E-5	0.0	0.0	19.063059738961684	0.0
14	5.8382160280047546E-5	0.0	0.0	19.359816259665166	0.0
15	5.8382160280047546E-5	0.0	0.0	19.922795431245664	0.0
16	5.8382160280047546E-5	0.0	0.0	21.061305938866873	0.0
17	5.8382160280047546E-5	0.0	0.0	22.527807422941386	0.0
18	5.8382160280047546E-5	0.0	0.0	24.0897053569135	0.0
19	5.8382160280047546E-5	0.0	0.0	25.076130337005182	0.0
20	5.8382160280047546E-5	0.0	0.0	26.02028663305411	0.0
21	5.8382160280047546E-5	0.0	0.0	27.13742927001282	0.0
22	1.1676432056009509E-4	0.0	0.0	28.384647360075476	0.0
23	1.1676432056009509E-4	0.0	0.0	29.570622564004363	0.0
24	1.1676432056009509E-4	0.0	0.0	30.46381123412881	0.0
25	1.7514648084014265E-4	0.0	0.0	31.26055257547062	0.0
26	1.7514648084014265E-4	0.0	0.0	32.01660155109723	0.0
27	1.7514648084014265E-4	0.0	0.0	32.76471055292576	0.0
28	1.7514648084014265E-4	0.0	0.0	33.50779868897021	0.0
29	1.7514648084014265E-4	0.0	0.0	34.312188093308706	0.0
30	1.7514648084014265E-4	0.0	0.0	35.19831252203927	0.0
31	1.7514648084014265E-4	0.0	0.0	35.99114225864231	0.0
32	2.3352864112019018E-4	0.0	0.0	36.71473075315322	0.0
33	2.3352864112019018E-4	0.0	0.0	37.41543344083435	0.0
34	2.3352864112019018E-4	0.0	0.0	38.13224960475277	0.0
35	2.3352864112019018E-4	0.0	0.0	38.89139283487423	0.0
36	2.3352864112019018E-4	0.0	0.0	39.6616286754489	0.0
37	2.3352864112019018E-4	0.0	0.0	40.37564249567388	0.0
38	2.3352864112019018E-4	0.0	0.0	41.12153297541177	0.0
39	2.3352864112019018E-4	0.0	0.0	41.95651463173701	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	30	2.1661272E-6	45.000004	37
TCGTTAA	20	7.0342555E-4	45.000004	27
GATCGGT	20	7.0342555E-4	45.000004	25
ACGTTCA	20	7.0342555E-4	45.000004	11
TTTCGTA	20	7.0342555E-4	45.000004	12
CGCATGA	20	7.0342555E-4	45.000004	43
GAATCGT	20	7.0342555E-4	45.000004	8
TATATCG	30	2.1661272E-6	45.000004	40
ATATCGC	30	2.1661272E-6	45.000004	41
CCGTAGA	20	7.0342555E-4	45.000004	21
AACGCTC	20	7.0342555E-4	45.000004	22
ACTAACG	20	7.0342555E-4	45.000004	40
CGTACCA	20	7.0342555E-4	45.000004	22
ACGTCGA	20	7.0342555E-4	45.000004	29
CGTCGGT	20	7.0342555E-4	45.000004	25
TCGAGTA	30	2.1661272E-6	45.000004	38
ACGTAAT	20	7.0342555E-4	45.000004	42
CGACTAA	20	7.0342555E-4	45.000004	29
TCGAACG	30	2.1661272E-6	45.000004	1
AATCGTC	20	7.0342555E-4	45.000004	36
>>END_MODULE