##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935035.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4739398
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47556525111417	33.0	31.0	34.0	31.0	34.0
2	32.693258299893785	34.0	31.0	34.0	31.0	34.0
3	32.598507236573084	34.0	31.0	34.0	31.0	34.0
4	36.14251409145212	37.0	35.0	37.0	35.0	37.0
5	36.081619015748416	37.0	35.0	37.0	35.0	37.0
6	36.03486835247852	37.0	35.0	37.0	35.0	37.0
7	36.23886134905741	37.0	37.0	37.0	35.0	37.0
8	36.050901612398874	37.0	37.0	37.0	35.0	37.0
9	37.80792117479899	39.0	38.0	39.0	35.0	39.0
10	37.68237527213372	39.0	37.0	39.0	35.0	39.0
11	37.57503294722241	39.0	37.0	39.0	35.0	39.0
12	37.32822206533404	39.0	37.0	39.0	35.0	39.0
13	37.2375421941774	39.0	37.0	39.0	34.0	39.0
14	38.26148342046817	40.0	38.0	41.0	33.0	41.0
15	38.372996739248315	40.0	38.0	41.0	33.0	41.0
16	38.47009198214626	40.0	38.0	41.0	34.0	41.0
17	38.50234206960462	40.0	38.0	41.0	34.0	41.0
18	38.379516765631415	40.0	37.0	41.0	34.0	41.0
19	38.23940213503909	40.0	37.0	41.0	34.0	41.0
20	38.08259825403986	40.0	35.0	41.0	34.0	41.0
21	38.0179385651933	40.0	35.0	41.0	34.0	41.0
22	38.00976643025127	40.0	35.0	41.0	34.0	41.0
23	37.84771082740888	40.0	35.0	41.0	34.0	41.0
24	37.65835323389173	40.0	35.0	41.0	33.0	41.0
25	37.60163632596376	39.0	35.0	41.0	33.0	41.0
26	37.71895650038254	40.0	35.0	41.0	34.0	41.0
27	37.75134415805552	40.0	35.0	41.0	34.0	41.0
28	37.68590504532432	40.0	35.0	41.0	34.0	41.0
29	37.65964263815784	40.0	35.0	41.0	34.0	41.0
30	37.52287716710013	40.0	35.0	41.0	33.0	41.0
31	37.31719788040591	40.0	35.0	41.0	33.0	41.0
32	37.086064517054695	39.0	35.0	41.0	33.0	41.0
33	36.788266568876466	39.0	35.0	41.0	32.0	41.0
34	36.4336348202873	39.0	35.0	41.0	31.0	41.0
35	36.234737829572445	39.0	35.0	41.0	30.0	41.0
36	36.111971815829776	39.0	35.0	41.0	30.0	41.0
37	36.04341521855729	39.0	35.0	41.0	30.0	41.0
38	35.95134192148454	39.0	35.0	41.0	29.0	41.0
39	35.838318073308045	39.0	35.0	41.0	29.0	41.0
40	35.7552132148429	39.0	35.0	41.0	29.0	41.0
41	35.62855767757846	39.0	35.0	41.0	27.0	41.0
42	35.590646533589286	38.0	35.0	41.0	28.0	41.0
43	35.53459046908489	38.0	35.0	41.0	27.0	41.0
44	35.409361062312136	38.0	35.0	41.0	27.0	41.0
45	35.369933692000544	38.0	35.0	41.0	27.0	41.0
46	35.297903868803594	38.0	35.0	40.0	27.0	41.0
47	35.234145982253445	38.0	35.0	40.0	27.0	41.0
48	35.11303271006149	37.0	35.0	40.0	26.0	41.0
49	35.04016480574115	37.0	35.0	40.0	26.0	41.0
50	34.96399226230842	37.0	34.0	40.0	26.0	41.0
51	33.95815375708054	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	42.0
9	71.0
10	93.0
11	82.0
12	62.0
13	111.0
14	151.0
15	236.0
16	421.0
17	771.0
18	1403.0
19	2398.0
20	4087.0
21	6439.0
22	9780.0
23	14934.0
24	24280.0
25	42216.0
26	64214.0
27	75441.0
28	75514.0
29	73240.0
30	77043.0
31	87777.0
32	110700.0
33	164867.0
34	340241.0
35	352568.0
36	331300.0
37	502316.0
38	889953.0
39	1485336.0
40	1308.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.659951327151674	20.729742469402233	23.785257114933163	18.82504908851293
2	37.812734866326906	22.455721169650662	23.53879121356763	16.192752750454805
3	29.17406387899898	21.927362926683937	32.80773212125253	16.090841073064553
4	26.06611641394118	23.93257540303642	32.39943132018033	17.601876862842076
5	24.404344180421226	27.338324403225894	30.42650142486451	17.83082999148837
6	25.99558002936238	30.53978585465918	28.329336341872956	15.135297774105489
7	76.2852370701933	4.970272595802252	13.611939744246	5.132550589758446
8	79.01488754478945	3.3356768095863654	13.312028236497547	4.337407409126644
9	73.14101917585315	5.4912670343364285	15.26012375411392	6.107590035696517
10	42.78750170380289	22.54396022448421	20.906030681533817	13.76250739017909
11	31.658303438537978	20.695434314653465	28.863940103785335	18.78232214302323
12	27.994821283209387	19.30126990811913	32.65157304788499	20.052335760786498
13	25.939602455839328	19.053685721266707	34.87999952736614	20.12671229552783
14	20.346930137540674	21.3935187549136	37.24156527896581	21.017985828579917
15	19.737253549923427	21.907022790658225	37.76361470380837	20.592108955609977
16	25.62734760828274	22.800342997148583	32.84874998892264	18.723559405646036
17	25.641463325088964	23.202651475989143	30.80581120218222	20.35007399673967
18	25.646337361833716	22.66258288499932	31.9285276315684	19.762552121598567
19	24.587363205200322	25.43156324917215	29.70788695104315	20.273186594584374
20	24.225798297589694	26.90145457292255	29.463615421199062	19.40913170828869
21	23.8784757051423	25.298339578148955	32.13471415567969	18.688470561029057
22	23.448463285843477	22.250505232943087	33.030925024655026	21.27010645655841
23	21.860286897196648	23.086138788090807	33.65499162551868	21.39858268919386
24	22.80272726620554	23.121290931886286	32.38691918256285	21.689062619345325
25	24.962305339201308	23.96441488982356	29.46439611106727	21.608883659907864
26	22.371702060050662	23.628633847589924	29.923420653846755	24.076243438512655
27	21.81990202131157	22.676424305365366	31.999106215599532	23.50456745772353
28	21.421644689895214	23.766478358643862	30.13870115993635	24.673175791524578
29	23.079133679003114	24.37066901745749	29.743207892647973	22.806989410891426
30	25.357883005394356	22.20461332852822	31.057045641661663	21.38045802441576
31	25.51967570564869	23.056134977480262	30.525564639222114	20.898624677648932
32	27.301188885170646	22.752298920664607	28.979440004827616	20.967072189337127
33	27.518790361138695	23.114243623346255	29.016195727811844	20.350770287703206
34	23.767491145499914	24.058414169900903	31.59156500466937	20.582529679929813
35	24.25911476520858	25.925634437116273	29.353580349234225	20.46167044844092
36	25.83306993841834	27.110236363352474	26.955723068626014	20.100970629603168
37	24.59331332797963	28.269202122294857	26.67117216152769	20.466312388197828
38	24.99741528354445	26.184886772539468	27.88799336962205	20.929704574294032
39	24.210754192832084	24.729174464773795	27.572193768069276	23.487877574324838
40	26.014780780175034	23.319417360601495	28.812245774674338	21.853556084549133
41	22.983699617546364	24.670897021098458	28.58702729755973	23.758376063795446
42	22.045647991580367	24.891304760646815	30.088652609466433	22.974394638306382
43	22.58103244336095	23.674378053921615	30.671279348136622	23.073310154580813
44	23.794266698006794	23.185666196424105	29.264307407818464	23.75575969775064
45	22.617788166345175	23.193641892915515	28.42780876389786	25.760761176841445
46	22.723603293076465	23.388561163253225	28.960323652919634	24.92751189075068
47	22.28683896140396	24.268461943900892	30.58162661164983	22.863072483045315
48	21.88326449899333	24.27525605572691	30.503578724555314	23.33790072072445
49	23.37630222234976	22.871322475976907	30.339992547576717	23.412382754096615
50	21.020053601744358	23.57326816612574	31.24270213221173	24.163976099918173
51	20.90685357085436	23.184526811211047	30.600025572868116	25.308594045066485
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2388.0
1	3368.5
2	4349.0
3	53461.0
4	102573.0
5	67517.0
6	32461.0
7	31797.5
8	31134.0
9	30931.5
10	30729.0
11	30039.0
12	29349.0
13	27961.0
14	26573.0
15	25108.5
16	23644.0
17	21886.5
18	20129.0
19	20048.0
20	19967.0
21	19778.5
22	19590.0
23	20337.0
24	21084.0
25	23250.0
26	30687.0
27	35958.0
28	40422.5
29	44887.0
30	51859.0
31	58831.0
32	66344.5
33	73858.0
34	86887.5
35	99917.0
36	111096.0
37	122275.0
38	128826.0
39	135377.0
40	148256.0
41	161135.0
42	174905.0
43	188675.0
44	209641.0
45	230607.0
46	288870.0
47	347133.0
48	372777.5
49	398422.0
50	404643.5
51	410865.0
52	371409.0
53	331953.0
54	313053.0
55	294153.0
56	285640.5
57	277128.0
58	267005.5
59	256883.0
60	242698.5
61	228514.0
62	211219.5
63	193925.0
64	172927.5
65	151930.0
66	129754.5
67	107579.0
68	91490.5
69	75402.0
70	62604.5
71	49807.0
72	40636.5
73	31466.0
74	25370.5
75	15773.0
76	12271.0
77	9327.0
78	6383.0
79	4682.5
80	2982.0
81	2091.0
82	1200.0
83	932.5
84	665.0
85	432.5
86	200.0
87	213.5
88	227.0
89	157.0
90	87.0
91	55.5
92	24.0
93	19.0
94	14.0
95	8.0
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4739398.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.796532623946778
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.40539924742893	15.999831794704228
2	9.774564328020993	4.26103220521152
3	3.624386725890943	2.3699719053804476
4	1.892863816731023	1.6503147173626462
5	1.1621517788149125	1.266543958045851
6	0.8470664418070272	1.1077866800098428
7	0.6349287675803141	0.9687471917512646
8	0.48901869280239063	0.8527129513109691
9	0.42617421919632453	0.836020824497796
>10	5.5946365486899285	30.54278652276202
>50	1.962752030853449	29.25628043779625
>100	0.1787755457828001	5.395106111265357
>500	0.0045265593861926745	0.6820851263856662
>1k	0.0016728589018575914	0.7480393965328747
>5k	5.904207888909146E-4	0.8791543417962495
>10k+	4.920173240757622E-4	3.1835858351868924
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	59036	1.245643434039513	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTT	32625	0.6883785662229676	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTC	23233	0.4902099380554239	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCT	18680	0.39414288481364085	No Hit
CGCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTG	14855	0.3134364322219826	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCT	8425	0.1777651929633257	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTC	8418	0.17761749488015144	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGTCTTCTGC	6136	0.12946791976533728	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCCGT	6111	0.12894042661114344	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGCC	6052	0.12769554276724598	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	5847	0.12337009890285644	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4769808317427656E-4	0.0	0.0	0.09591935515860875	0.0
2	1.4769808317427656E-4	0.0	0.0	0.3408449765138948	0.0
3	1.4769808317427656E-4	0.0	0.0	0.8092166979856935	0.0
4	1.4769808317427656E-4	0.0	0.0	1.1975360583770345	0.0
5	1.4769808317427656E-4	0.0	0.0	2.344664870939305	0.0
6	1.4769808317427656E-4	0.0	0.0	3.982721012246703	0.0
7	1.4769808317427656E-4	0.0	0.0	5.239842697321474	0.0
8	1.4769808317427656E-4	0.0	0.0	6.5749700700384315	0.0
9	1.4769808317427656E-4	0.0	0.0	7.582988387976701	0.0
10	1.8989753550978416E-4	0.0	0.0	8.695745746611701	0.0
11	1.8989753550978416E-4	0.0	0.0	9.80088610410014	0.0
12	1.8989753550978416E-4	0.0	0.0	10.493780011723008	0.0
13	1.8989753550978416E-4	0.0	0.0	10.843507972953526	0.0
14	1.8989753550978416E-4	0.0	0.0	11.121011571511826	0.0
15	2.1099726167753794E-4	0.0	0.0	11.336566374041597	0.0
16	2.1099726167753794E-4	0.0	0.0	11.618163319476439	0.0
17	2.1099726167753794E-4	0.0	0.0	11.92955307826015	0.0
18	2.1099726167753794E-4	0.0	0.0	12.283184488831704	0.0
19	2.1099726167753794E-4	0.0	0.0	12.540369051090455	0.0
20	2.1099726167753794E-4	0.0	0.0	12.818885436504805	0.0
21	2.3209698784529175E-4	0.0	0.0	13.14217544084713	0.0
22	2.5319671401304554E-4	0.0	0.0	13.509416174796883	0.0
23	2.5319671401304554E-4	0.0	0.0	13.85268761982007	0.0
24	2.5319671401304554E-4	0.0	0.0	14.143673943399563	0.0
25	2.5319671401304554E-4	0.0	0.0	14.408074612007685	0.0
26	2.5319671401304554E-4	0.0	0.0	14.674648552411087	0.0
27	2.5319671401304554E-4	2.1099726167753796E-5	0.0	14.992072832878774	0.0
28	2.953961663485531E-4	2.1099726167753796E-5	0.0	15.271791902684686	0.0
29	2.953961663485531E-4	2.1099726167753796E-5	0.0	15.58419444832445	0.0
30	2.953961663485531E-4	2.1099726167753796E-5	0.0	15.972935803239146	0.0
31	3.164958925163069E-4	2.1099726167753796E-5	0.0	16.272045521393224	0.0
32	3.164958925163069E-4	2.1099726167753796E-5	0.0	16.589933995836603	0.0
33	3.164958925163069E-4	2.1099726167753796E-5	0.0	16.890963789071947	0.0
34	3.164958925163069E-4	2.1099726167753796E-5	0.0	17.20304983881919	0.0
35	3.164958925163069E-4	2.1099726167753796E-5	0.0	17.55957191187573	0.0
36	3.164958925163069E-4	2.1099726167753796E-5	0.0	17.895542851644873	0.0
37	3.164958925163069E-4	2.1099726167753796E-5	0.0	18.283102621894173	0.0
38	3.164958925163069E-4	2.1099726167753796E-5	0.0	18.790740933764162	0.0
39	3.164958925163069E-4	2.1099726167753796E-5	0.0	19.6403003081826	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	35330	0.0	43.860035	1
CGTTTCT	3945	0.0	39.23954	1
CGTTCTG	2990	0.0	39.20569	1
TAATGCG	140	0.0	38.571426	1
GTTTTTT	40530	0.0	38.15507	2
TATACGG	375	0.0	36.0	2
TAATACG	120	0.0	35.625	1
CGTAAGG	670	0.0	35.59701	2
CGTTTTC	2405	0.0	35.457382	1
TTGTACG	240	0.0	34.6875	1
CGGTCTA	280	0.0	34.55357	31
TACGGGA	1285	0.0	34.319065	4
GCGTAAG	420	0.0	34.285713	1
TACGAAT	430	0.0	34.011627	12
TAAGGGA	3145	0.0	33.767887	4
GACCGAT	1890	0.0	33.69048	9
TTATGCG	195	0.0	33.461536	1
CTATGCG	190	0.0	33.157894	1
ACGGGAG	2385	0.0	33.11321	5
TTACGGG	1110	0.0	33.04054	3
>>END_MODULE