##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935032.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1188777
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.37145654735917	33.0	31.0	34.0	31.0	34.0
2	32.54757620647102	34.0	31.0	34.0	31.0	34.0
3	32.55228861258251	34.0	31.0	34.0	31.0	34.0
4	36.066558320021336	37.0	35.0	37.0	35.0	37.0
5	35.98453789062204	37.0	35.0	37.0	35.0	37.0
6	35.927963781264275	37.0	35.0	37.0	35.0	37.0
7	36.15807169889727	37.0	35.0	37.0	35.0	37.0
8	35.87456941041087	37.0	35.0	37.0	35.0	37.0
9	37.625285482474844	39.0	38.0	39.0	35.0	39.0
10	37.50415090466925	39.0	37.0	39.0	35.0	39.0
11	37.434132726322936	39.0	37.0	39.0	35.0	39.0
12	37.23537803978375	39.0	37.0	39.0	35.0	39.0
13	37.13307205640755	39.0	37.0	39.0	33.0	39.0
14	38.31436005238998	40.0	37.0	41.0	34.0	41.0
15	38.410377219613096	40.0	37.0	41.0	34.0	41.0
16	38.45625041534283	40.0	37.0	41.0	34.0	41.0
17	38.4797064546168	40.0	37.0	41.0	35.0	41.0
18	38.48210135290302	40.0	37.0	41.0	35.0	41.0
19	38.443438088051835	40.0	37.0	41.0	34.0	41.0
20	38.43471651958273	40.0	37.0	41.0	34.0	41.0
21	38.36085573660998	40.0	37.0	41.0	34.0	41.0
22	38.28427114589196	40.0	37.0	41.0	34.0	41.0
23	38.09801585999729	40.0	36.0	41.0	34.0	41.0
24	37.909783752545685	40.0	36.0	41.0	34.0	41.0
25	37.83963266449469	40.0	36.0	41.0	34.0	41.0
26	37.974073354380174	40.0	36.0	41.0	34.0	41.0
27	38.01571615197804	40.0	36.0	41.0	34.0	41.0
28	37.9917654867145	40.0	36.0	41.0	34.0	41.0
29	38.020692695097566	40.0	36.0	41.0	34.0	41.0
30	37.96448198442601	40.0	36.0	41.0	34.0	41.0
31	37.86262604340427	40.0	36.0	41.0	34.0	41.0
32	37.75743305935428	40.0	36.0	41.0	34.0	41.0
33	37.709064862459485	40.0	36.0	41.0	33.0	41.0
34	37.572087952576474	40.0	35.0	41.0	33.0	41.0
35	37.48613827488251	40.0	35.0	41.0	33.0	41.0
36	37.446572401720424	40.0	35.0	41.0	33.0	41.0
37	37.3488265671358	40.0	35.0	41.0	33.0	41.0
38	37.30546603778505	39.0	35.0	41.0	33.0	41.0
39	37.258130835303845	39.0	35.0	41.0	33.0	41.0
40	37.168216578887375	39.0	35.0	41.0	33.0	41.0
41	37.139104306358554	39.0	35.0	41.0	33.0	41.0
42	37.13796616186215	39.0	35.0	41.0	33.0	41.0
43	37.04841698653322	39.0	35.0	41.0	33.0	41.0
44	36.92820099985111	39.0	35.0	41.0	32.0	41.0
45	36.93514090531698	39.0	35.0	41.0	32.0	41.0
46	36.86501673568718	39.0	35.0	41.0	32.0	41.0
47	36.809374676663495	39.0	35.0	41.0	32.0	41.0
48	36.75128051770854	39.0	35.0	41.0	32.0	41.0
49	36.67181565592201	39.0	35.0	41.0	32.0	41.0
50	36.581183855340406	39.0	35.0	41.0	32.0	41.0
51	35.64315006094499	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	12.0
10	21.0
11	27.0
12	25.0
13	26.0
14	34.0
15	47.0
16	78.0
17	132.0
18	250.0
19	438.0
20	750.0
21	1193.0
22	1934.0
23	2648.0
24	3779.0
25	4903.0
26	6105.0
27	7357.0
28	8831.0
29	10947.0
30	13636.0
31	17799.0
32	23788.0
33	34845.0
34	72897.0
35	112608.0
36	79669.0
37	127388.0
38	240566.0
39	415661.0
40	372.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.93940495147534	22.438522952580676	25.45986337218839	10.16220872375559
2	30.83050900210889	24.547581253674995	26.607092835746315	18.014816908469797
3	31.694506202593086	24.200501860315267	26.785259136070096	17.319732801021555
4	28.94150879433233	26.509681799025387	25.73131882598671	18.817490580655583
5	25.751928242218685	30.255968949601147	24.2086615067418	19.78344130143837
6	26.901513067631694	35.114659856306105	22.698958677699853	15.28486839836235
7	80.26046937314568	6.589461269859696	8.003940183903289	5.146129173091336
8	80.87151753440722	5.466963105780142	7.503846390029417	6.157672969783231
9	75.11863032343325	8.032120406098032	10.078004537436373	6.7712447330323515
10	41.21285993924849	28.701934845643883	16.32467653731524	13.760528677792387
11	33.51730391822856	24.124457320422586	23.50710015419208	18.851138607156766
12	29.639284743900664	22.71788569260677	26.9614065548038	20.681423008688764
13	24.263003069541217	26.91909416147856	28.777138184874033	20.040764584106185
14	19.389507031175736	30.55518402526294	28.067080705632762	21.98822823792856
15	18.202152296015147	26.258246920995276	35.3401857539303	20.199415029059274
16	20.760495870966547	25.923364937242226	31.776102666858456	21.540036524932766
17	20.518566560423025	25.3564798107635	27.43348836661544	26.69146526219804
18	21.60968793979022	26.25126495549628	29.21489900965446	22.924148095059042
19	23.66061927510374	27.970090269243098	26.748162186852536	21.621128268800625
20	26.662275599208265	26.62450568946068	26.434730819993995	20.278487891337065
21	23.53965461983198	28.083988838949608	26.814617039192377	21.561739502026033
22	21.533979880162555	26.755732992815307	26.156714001027947	25.553573125994195
23	21.393247009321346	27.01869231992207	27.13107672843603	24.456983942320555
24	22.363319613350527	24.07743420338718	29.424610334823097	24.134635848439196
25	20.780011726337236	27.323964040354078	27.026515486083596	24.86950874722509
26	20.582666050907783	29.449425754367724	27.534095965853982	22.43381222887051
27	22.37274106077086	27.933077440091793	27.644882093109135	22.04929940602821
28	18.4021898135647	27.057135190199677	29.98451349580283	24.556161500432797
29	20.25829907543635	26.06460252848095	29.599159472298	24.077938923784696
30	22.603145922237726	26.33050605790657	27.125945404394603	23.9404026154611
31	22.429606225557862	29.450014594831497	24.35309566049814	23.7672835191125
32	23.487668418887647	29.91334791975282	24.039832533772103	22.55915112758743
33	24.281425364050616	28.40213092951832	23.84787054258284	23.468573163848223
34	21.29642481306418	28.008364899388194	25.924290257971006	24.770920029576615
35	21.476778235110537	27.51054234730315	26.72368324757293	24.288996170013384
36	24.38741664752935	27.61005638568041	25.717943735452486	22.284583231337756
37	21.98906943859109	29.63768646264186	26.197175752895625	22.176068345871432
38	21.148962336922736	29.267474051062564	24.80263329455398	24.78093031746072
39	23.196360629453633	27.504317462400437	25.80610156488559	23.493220343260344
40	21.98427459481467	26.485286979812024	28.319356784325407	23.2110816410479
41	19.717995889893565	28.48978403855391	26.56721992434241	25.225000147210114
42	23.505501872933273	25.820401976148595	26.5654534029511	24.10864274796703
43	24.260395347487375	25.821074936678617	26.2533679571526	23.665161758681403
44	21.329483999101598	26.500596831870066	25.541964556851287	26.627954612177053
45	21.084778726371724	27.021300041975916	25.495446160213397	26.398475071438966
46	22.111211774790394	27.62561859793721	26.026496138468357	24.23667348880404
47	20.561720154410793	26.370210729177973	28.977007462291077	24.091061654120157
48	21.396359451772703	24.750226493278387	28.46395917821425	25.389454876734664
49	21.802238771443257	24.450254337020315	29.460024882715597	24.28748200882083
50	19.914416244594236	24.865723344243705	28.738106474132657	26.4817539370294
51	19.755934039773653	25.65569488642529	25.767406334409227	28.82096473939183
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	849.0
1	1168.0
2	1487.0
3	1958.5
4	2430.0
5	1942.0
6	1454.0
7	1537.5
8	1621.0
9	1597.5
10	1574.0
11	1682.5
12	1791.0
13	1769.5
14	1748.0
15	1880.5
16	2013.0
17	2269.5
18	2526.0
19	2487.5
20	2449.0
21	3041.0
22	3633.0
23	4292.5
24	4952.0
25	6250.0
26	9015.5
27	10483.0
28	13048.5
29	15614.0
30	17298.0
31	18982.0
32	21551.5
33	24121.0
34	27962.5
35	31804.0
36	34436.5
37	37069.0
38	38923.0
39	40777.0
40	47122.5
41	53468.0
42	62857.5
43	72247.0
44	79124.0
45	86001.0
46	93589.5
47	101178.0
48	113084.0
49	124990.0
50	119242.0
51	113494.0
52	100713.0
53	87932.0
54	77293.5
55	66655.0
56	59030.0
57	51405.0
58	50683.5
59	49962.0
60	49605.5
61	49249.0
62	44696.5
63	40144.0
64	32688.5
65	25233.0
66	21482.0
67	17731.0
68	14958.0
69	12185.0
70	10189.0
71	8193.0
72	7015.0
73	5837.0
74	4534.0
75	2713.5
76	2196.0
77	1670.0
78	1144.0
79	959.0
80	774.0
81	516.5
82	259.0
83	262.5
84	266.0
85	147.0
86	28.0
87	26.0
88	24.0
89	20.0
90	16.0
91	11.0
92	6.0
93	4.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1188777.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.229490625267186
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.26195895836466	12.741837186017124
2	12.680550469918689	4.876810527690576
3	5.892973508754058	3.3995663652460215
4	3.2712028216107276	2.516142559660442
5	2.085361434054819	2.005021907322544
6	1.4120379150285642	1.629166190973816
7	0.9919095529789099	1.3351740815084638
8	0.7129979743773592	1.096847029129914
9	0.5342803515480552	0.9246545110222005
>10	3.515946816287979	14.056879011071851
>50	1.4118408973834227	19.97894214794381
>100	1.2174249134614175	32.85026735009814
>500	0.008414359169067636	1.1125291450939012
>1k	0.003100027062288077	1.4761619872211944
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	4589	0.38602698403485264	No Hit
CCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	3789	0.3187309310324813	No Hit
CTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGCT	3387	0.28491466439878965	No Hit
TCTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	1718	0.14451827382259247	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1408	0.11844105328417356	No Hit
ACTGTCTCTTATACACATCTGACGCTGCACAAGTCGTATGCCGTCTTCTGC	1382	0.1162539315615965	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001850641457565212	0.0	0.0	0.30064511678809397	0.0
2	0.001850641457565212	0.0	0.0	1.3277511257367867	0.0
3	0.001850641457565212	0.0	0.0	1.8563616220704136	0.0
4	0.001850641457565212	0.0	0.0	2.4934028838041113	0.0
5	0.001850641457565212	0.0	0.0	4.162176758130415	0.0
6	0.001850641457565212	0.0	0.0	5.001274419003733	0.0
7	0.001850641457565212	0.0	0.0	5.921127343479895	0.0
8	0.001850641457565212	0.0	0.0	7.314576240960247	0.0
9	0.001850641457565212	0.0	0.0	7.759571391438428	0.0
10	0.001850641457565212	0.0	0.0	8.91268925963406	0.0
11	0.001850641457565212	0.0	0.0	10.609306875890095	0.0
12	0.001850641457565212	0.0	0.0	11.903830575456961	0.0
13	0.001850641457565212	0.0	0.0	12.388278037007781	0.0
14	0.001850641457565212	0.0	0.0	12.583604830847165	0.0
15	0.001850641457565212	0.0	0.0	12.986287588000105	0.0
16	0.001850641457565212	0.0	0.0	13.804271112243928	0.0
17	0.001850641457565212	0.0	0.0	14.786457005813539	0.0
18	0.0019347615238181762	0.0	0.0	15.883466789818444	0.0
19	0.0019347615238181762	0.0	0.0	16.541033347717864	0.0
20	0.0019347615238181762	0.0	0.0	17.17546688739772	0.0
21	0.0019347615238181762	0.0	0.0	17.94230541135974	0.0
22	0.0019347615238181762	0.0	0.0	18.712845218236893	0.0
23	0.0019347615238181762	0.0	0.0	19.501470839358433	0.0
24	0.0019347615238181762	0.0	0.0	20.1489429893075	0.0
25	0.0019347615238181762	0.0	0.0	20.71179035260608	0.0
26	0.0019347615238181762	0.0	0.0	21.235690125229542	0.0
27	0.0019347615238181762	0.0	0.0	21.705668935384853	0.0
28	0.0019347615238181762	0.0	0.0	22.184985072894243	0.0
29	0.0019347615238181762	0.0	0.0	22.723353496913216	0.0
30	0.0019347615238181762	0.0	0.0	23.314465202472793	0.0
31	0.0019347615238181762	0.0	0.0	23.876219004910087	0.0
32	0.0019347615238181762	0.0	0.0	24.384304205077992	0.0
33	0.0019347615238181762	0.0	0.0	24.89676364869105	0.0
34	0.0019347615238181762	0.0	0.0	25.3877724754096	0.0
35	0.0019347615238181762	0.0	0.0	25.947255036058067	0.0
36	0.0019347615238181762	0.0	0.0	26.515990803994356	0.0
37	0.0019347615238181762	0.0	0.0	27.030216768998727	0.0
38	0.0019347615238181762	0.0	0.0	27.542087372148014	0.0
39	0.0019347615238181762	0.0	0.0	28.053453254899782	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATCG	20	7.0333836E-4	45.000004	32
AGATCGC	30	2.165565E-6	45.000004	26
GGTAATC	20	7.0333836E-4	45.000004	8
TCCGCAT	20	7.0333836E-4	45.000004	32
TCACACG	30	2.165565E-6	45.000004	1
ATTACGG	60	0.0	45.000004	2
GCCGGAT	60	0.0	45.000004	19
CGATTCT	35	1.2119744E-7	45.000004	10
CGACACG	20	7.0333836E-4	45.000004	12
TCTAGCG	30	2.165565E-6	45.000004	1
CACCTCG	20	7.0333836E-4	45.000004	12
ACGATTC	20	7.0333836E-4	45.000004	1
CTAACGT	20	7.0333836E-4	45.000004	45
ACGATAG	20	7.0333836E-4	45.000004	1
GCCGAAC	30	2.165565E-6	45.000004	9
CGGATTA	20	7.0333836E-4	45.000004	7
CACTACG	20	7.0333836E-4	45.000004	12
TATTCGC	35	1.2119744E-7	45.000004	13
AGCGCAT	20	7.0333836E-4	45.000004	10
GCTAGAT	20	7.0333836E-4	45.000004	36
>>END_MODULE