SRR2935031.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935031.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1688409
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	4912	0.2909247700053719	RNA PCR Primer, Index 23 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	4893	0.28979945025168663	RNA PCR Primer, Index 30 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT	3523	0.20865797327543267	RNA PCR Primer, Index 30 (95% over 24bp)
GCCCTGCTAGAGGACTTTCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC	2412	0.1428563813625727	No Hit
TCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC	2214	0.13112936498206298	RNA PCR Primer, Index 23 (96% over 25bp)
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	2111	0.1250289473699797	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCC	1794	0.10625387569007272	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	30	2.166109E-6	45.000004	1
CTATCCG	25	3.8915852E-5	45.0	1
CTGCGTA	20	7.034228E-4	45.0	16
CGGATAG	20	7.034228E-4	45.0	2
ACCGTTC	20	7.034228E-4	45.0	11
CGCGCAT	215	0.0	43.953487	36
ATATGCG	55	6.184564E-11	40.909092	1
ACTTTCG	365	0.0	40.68493	14
TACGCGC	240	0.0	39.375004	34
CTTTCGT	380	0.0	39.078945	15
ACGCGAG	75	0.0	39.0	1
ACGGGTA	185	0.0	38.91892	5
CGTAAGG	180	0.0	38.75	2
CGTTTTT	1415	0.0	38.639576	1
TACGATA	35	6.2500294E-6	38.57143	23
CTACGAT	35	6.2500294E-6	38.57143	22
CCCTCGT	340	0.0	38.382355	14
TTTCGTC	390	0.0	38.076923	16
TTCGTCT	385	0.0	37.987015	17
TTTAGCG	60	1.5643309E-10	37.500004	1