##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2935031.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1688409 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3742920109997 33.0 31.0 34.0 31.0 34.0 2 32.55054195991611 34.0 31.0 34.0 31.0 34.0 3 32.54429643528316 34.0 31.0 34.0 31.0 34.0 4 36.04390168495904 37.0 35.0 37.0 35.0 37.0 5 35.97062323169327 37.0 35.0 37.0 35.0 37.0 6 35.91041151758845 37.0 35.0 37.0 35.0 37.0 7 36.13593092668897 37.0 35.0 37.0 35.0 37.0 8 35.86910102943067 37.0 35.0 37.0 35.0 37.0 9 37.583741854017596 39.0 38.0 39.0 35.0 39.0 10 37.46272200633851 39.0 37.0 39.0 35.0 39.0 11 37.393975630312326 39.0 37.0 39.0 35.0 39.0 12 37.304958691880934 39.0 37.0 39.0 35.0 39.0 13 37.266094885777086 39.0 37.0 39.0 34.0 39.0 14 38.483527984037046 40.0 38.0 41.0 34.0 41.0 15 38.54679464513634 40.0 38.0 41.0 34.0 41.0 16 38.58584442513633 40.0 38.0 41.0 35.0 41.0 17 38.58855407664849 40.0 38.0 41.0 35.0 41.0 18 38.586656432179645 40.0 38.0 41.0 35.0 41.0 19 38.55904760043331 40.0 38.0 41.0 34.0 41.0 20 38.52564574104971 40.0 38.0 41.0 34.0 41.0 21 38.467592864051305 40.0 38.0 41.0 34.0 41.0 22 38.385945585459446 40.0 37.0 41.0 34.0 41.0 23 38.19277793473027 40.0 37.0 41.0 34.0 41.0 24 37.98185629192927 40.0 37.0 41.0 33.0 41.0 25 37.91525453844418 40.0 37.0 41.0 33.0 41.0 26 38.07673140808892 40.0 37.0 41.0 34.0 41.0 27 38.099943793239675 40.0 37.0 41.0 34.0 41.0 28 38.02780132065157 40.0 37.0 41.0 34.0 41.0 29 38.07350174039584 40.0 37.0 41.0 34.0 41.0 30 37.9851907920415 40.0 37.0 41.0 34.0 41.0 31 37.88574510086122 40.0 36.0 41.0 34.0 41.0 32 37.83524667305137 40.0 36.0 41.0 33.0 41.0 33 37.75788686272106 40.0 36.0 41.0 33.0 41.0 34 37.63891213562591 40.0 36.0 41.0 33.0 41.0 35 37.5762270871572 40.0 36.0 41.0 33.0 41.0 36 37.49931918154902 40.0 36.0 41.0 33.0 41.0 37 37.41759964558351 40.0 35.0 41.0 33.0 41.0 38 37.355359394554284 40.0 35.0 41.0 33.0 41.0 39 37.295703825317204 40.0 35.0 41.0 33.0 41.0 40 37.25406047942175 39.0 35.0 41.0 33.0 41.0 41 37.195036866067404 39.0 35.0 41.0 33.0 41.0 42 37.1615491270184 39.0 35.0 41.0 33.0 41.0 43 37.08206720054205 39.0 35.0 41.0 32.0 41.0 44 36.9498516058609 39.0 35.0 41.0 32.0 41.0 45 36.9187299996624 39.0 35.0 41.0 32.0 41.0 46 36.79373955007347 39.0 35.0 41.0 32.0 41.0 47 36.722444620941964 39.0 35.0 41.0 31.0 41.0 48 36.670543689354886 39.0 35.0 41.0 31.0 41.0 49 36.584522470562526 39.0 35.0 41.0 31.0 41.0 50 36.512493714496905 39.0 35.0 40.0 31.0 41.0 51 35.48523432414776 37.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 10.0 9 22.0 10 32.0 11 29.0 12 32.0 13 23.0 14 39.0 15 58.0 16 116.0 17 174.0 18 322.0 19 621.0 20 1062.0 21 1592.0 22 2576.0 23 3606.0 24 4992.0 25 6825.0 26 8990.0 27 11037.0 28 13371.0 29 16369.0 30 20526.0 31 26338.0 32 34742.0 33 49762.0 34 93427.0 35 127506.0 36 124738.0 37 196777.0 38 360357.0 39 581855.0 40 481.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.83045044180646 21.651092833549217 26.16007140449974 11.358385320144587 2 31.304085680661498 24.468656587355316 25.63904835854346 18.588209373439728 3 31.30775777669984 23.99951670477947 27.036517810554194 17.65620770796649 4 28.520873793020527 25.774382865762973 26.381640941264823 19.32310239995167 5 26.090656943903994 29.352958909837607 24.817387256286835 19.738996889971567 6 27.503347826267216 33.15103153323632 23.307622738329396 16.03799790216707 7 81.12021435564488 5.974796391158777 7.538931621425851 5.366057631770501 8 81.02699049815536 4.837986530514822 8.208378420157675 5.926644551172139 9 74.9679135801811 7.485508546803529 10.330672248252645 7.215905624762721 10 40.75748233988329 26.24891243768542 17.219287506759322 15.774317715671973 11 28.968454918209986 24.490629936229908 24.996135415056422 21.544779730503688 12 26.333489101278186 21.833749997778973 30.07659873881269 21.756162162130146 13 23.321481939506363 23.18318606451399 32.11609272397861 21.379239272001037 14 20.378533874197544 25.8224162510387 30.635586519616986 23.163463355146767 15 19.062738945362174 25.023676135343987 33.865609576826465 22.047975342467378 16 22.417080221676148 24.58450529462944 30.246699703685543 22.75171478000887 17 22.14333138475334 24.89029613085455 27.93487833812779 25.031494146264322 18 22.797912117265426 24.87134337710827 29.325122052772755 23.005622452853544 19 23.312242472055054 25.91706156505918 26.85936879038195 23.911327172503817 20 24.48150892348951 26.43352410464526 27.737532789744662 21.347434182120566 21 24.858550268329534 25.59622698054796 28.416752102126914 21.12847064899559 22 23.561767320595898 24.15516619492078 27.41101237910956 24.872054105373756 23 21.6222491114416 25.055125861091714 28.455249883174044 24.867375144292645 24 23.278897470932694 23.31739525197982 29.183924037362985 24.2197832397245 25 22.021737623999872 26.177247337582305 27.130570851020103 24.67044418739772 26 21.718552791414876 26.662733970264313 26.89123310761788 24.72748013070293 27 23.005267088720803 26.009870831060482 27.220892568092207 23.763969512126504 28 20.594891403682404 25.24566026359727 28.859891175657083 25.299557157063248 29 22.874137723738738 23.488384627184526 27.973672255952202 25.663805393124534 30 25.049499262323287 24.048616182453422 27.2162136070111 23.68567094821219 31 23.37810329132337 26.35238262766901 26.476404709996217 23.793109371011408 32 25.200469791383483 25.616068144626098 25.41582045582557 23.767641608164848 33 25.681632827117124 25.89994485933207 25.137214975755285 23.28120733779552 34 23.353523938808664 25.62501147530012 27.668059101793464 23.35340548409775 35 23.703024563361126 24.89503431929112 27.003409718853668 24.398531398494086 36 24.383606104918893 26.9204914212137 26.401659787409333 22.29424268645808 37 24.0322102049918 26.936660489253494 27.715618668225527 21.31551063752918 38 23.89154523578114 26.105523010123733 27.24967706284437 22.753254691250756 39 24.790438809553848 26.6202087290461 25.72249970238254 22.866852759017515 40 24.09345129053446 25.176838076556095 27.7972931914009 22.932417441508544 41 21.54365441075 26.60599416373639 27.334431408503505 24.515920017010096 42 22.448115355935677 25.462136247793037 28.04089530439603 24.048853091875248 43 23.890360688672 24.363528031418927 28.417403603036938 23.32870767687213 44 24.01473813513195 23.517761395491256 27.40064759190457 25.066852877472222 45 24.576095010154532 22.763974842588496 26.520292180389944 26.139637966867035 46 24.313421688702206 24.205272537637505 27.66006340880675 23.82124236485354 47 21.14771953951916 25.035403151724495 30.002090725647633 23.814786583108713 48 22.00000118454711 23.467299688641795 29.59028292315428 24.942416203656816 49 22.470503296298467 23.513082434410144 30.427698501962496 23.58871576732889 50 21.971512826572233 22.830842526899584 29.874574229348454 25.32307041717972 51 21.536961719583346 22.996619894824065 27.347046835215878 28.119371550376716 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 761.0 1 1269.0 2 1777.0 3 2827.5 4 3878.0 5 3002.0 6 2126.0 7 2168.0 8 2210.0 9 2296.0 10 2382.0 11 2448.0 12 2514.0 13 2622.0 14 2730.0 15 2727.5 16 2725.0 17 2830.5 18 2936.0 19 3136.5 20 3337.0 21 3791.5 22 4246.0 23 5065.0 24 5884.0 25 7411.0 26 11652.0 27 14366.0 28 15775.0 29 17184.0 30 19536.5 31 21889.0 32 25962.0 33 30035.0 34 35154.0 35 40273.0 36 44695.0 37 49117.0 38 53633.5 39 58150.0 40 62531.5 41 66913.0 42 74599.0 43 82285.0 44 91622.5 45 100960.0 46 119420.5 47 137881.0 48 157887.5 49 177894.0 50 173263.0 51 168632.0 52 148422.5 53 128213.0 54 114939.0 55 101665.0 56 96075.5 57 90486.0 58 88305.5 59 86125.0 60 79550.0 61 72975.0 62 66276.5 63 59578.0 64 52173.0 65 44768.0 66 38232.5 67 31697.0 68 26756.5 69 21816.0 70 18457.0 71 15098.0 72 12709.0 73 10320.0 74 8422.0 75 5281.5 76 4039.0 77 2919.0 78 1799.0 79 1222.0 80 645.0 81 535.5 82 426.0 83 286.0 84 146.0 85 89.5 86 33.0 87 26.0 88 19.0 89 12.5 90 6.0 91 4.0 92 2.0 93 3.5 94 5.0 95 3.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1688409.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.6713475700729 #Duplication Level Percentage of deduplicated Percentage of total 1 69.55428845417262 15.76889448533656 2 12.149304525206604 5.508822112512369 3 4.889053335794764 3.3252428239328227 4 2.506762842828856 2.273267667420681 5 1.4541611007189914 1.648389586864002 6 0.9476338989447282 1.2890482495295563 7 0.6701464430668098 1.0635186053531 8 0.5201060404463768 0.9433203853003361 9 0.3790605898457191 0.7734432944258229 >10 4.726599364916565 27.071860183780565 >50 2.008846060812475 31.492506451271247 >100 0.1839643665344099 5.974887046643426 >500 0.005831723359049555 0.8601659356330393 >1k 0.00424125335203604 2.0066331719965893 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC 4912 0.2909247700053719 RNA PCR Primer, Index 23 (96% over 25bp) CCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC 4893 0.28979945025168663 RNA PCR Primer, Index 30 (95% over 23bp) CTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGCT 3523 0.20865797327543267 RNA PCR Primer, Index 30 (95% over 24bp) GCCCTGCTAGAGGACTTTCGTCTGTATCTATGCTCTGCCCCATGCAGCTCC 2412 0.1428563813625727 No Hit TCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCCGTCTTCTGC 2214 0.13112936498206298 RNA PCR Primer, Index 23 (96% over 25bp) TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC 2111 0.1250289473699797 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTGGATATCGTATGCC 1794 0.10625387569007272 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.7768206637135906E-4 0.0 0.0 0.2198519434568283 0.0 2 1.7768206637135906E-4 0.0 0.0 1.0676915368254967 0.0 3 1.7768206637135906E-4 0.0 0.0 1.597302549323061 0.0 4 1.7768206637135906E-4 0.0 0.0 2.26088583986463 0.0 5 1.7768206637135906E-4 0.0 0.0 3.960651714128508 0.0 6 1.7768206637135906E-4 0.0 0.0 4.913975227566307 0.0 7 1.7768206637135906E-4 0.0 0.0 5.89454332451438 0.0 8 1.7768206637135906E-4 0.0 0.0 7.227336504365945 0.0 9 2.3690942182847877E-4 0.0 0.0 7.687355374201394 0.0 10 2.3690942182847877E-4 0.0 0.0 8.709974893524022 0.0 11 2.3690942182847877E-4 0.0 0.0 10.374441263935456 0.0 12 2.3690942182847877E-4 0.0 0.0 11.586055274521755 0.0 13 2.3690942182847877E-4 0.0 0.0 12.068817448852737 0.0 14 2.3690942182847877E-4 0.0 0.0 12.264741540704888 0.0 15 2.3690942182847877E-4 0.0 0.0 12.620816401713093 0.0 16 2.3690942182847877E-4 0.0 0.0 13.397405486466845 0.0 17 2.3690942182847877E-4 0.0 0.0 14.307552257776404 0.0 18 2.3690942182847877E-4 0.0 0.0 15.233808869770298 0.0 19 2.3690942182847877E-4 0.0 0.0 15.83976394345209 0.0 20 2.3690942182847877E-4 0.0 0.0 16.436361094971655 0.0 21 2.3690942182847877E-4 0.0 0.0 17.149280772609007 0.0 22 2.3690942182847877E-4 0.0 0.0 17.909167743123852 0.0 23 2.3690942182847877E-4 0.0 0.0 18.63197838912254 0.0 24 2.3690942182847877E-4 0.0 0.0 19.214242520621486 0.0 25 2.3690942182847877E-4 0.0 0.0 19.727921374501083 0.0 26 2.3690942182847877E-4 0.0 0.0 20.197298166498758 0.0 27 2.3690942182847877E-4 0.0 0.0 20.663476681301745 0.0 28 2.3690942182847877E-4 0.0 0.0 21.173305757076633 0.0 29 2.961367772855985E-4 0.0 0.0 21.67318463713472 0.0 30 4.1459148819983783E-4 0.0 0.0 22.230928643474417 0.0 31 4.1459148819983783E-4 0.0 0.0 22.736078758168194 0.0 32 4.1459148819983783E-4 0.0 0.0 23.207943099095065 0.0 33 4.1459148819983783E-4 0.0 0.0 23.690112999871477 0.0 34 4.1459148819983783E-4 0.0 0.0 24.146578228379497 0.0 35 4.1459148819983783E-4 0.0 0.0 24.643614195375648 0.0 36 4.1459148819983783E-4 0.0 0.0 25.13745188517711 0.0 37 4.1459148819983783E-4 0.0 0.0 25.619562558598066 0.0 38 4.7381884365695754E-4 0.0 0.0 26.090656943903994 0.0 39 5.330461991140772E-4 0.0 0.0 26.54724062712293 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATACG 30 2.166109E-6 45.000004 1 CTATCCG 25 3.8915852E-5 45.0 1 CTGCGTA 20 7.034228E-4 45.0 16 CGGATAG 20 7.034228E-4 45.0 2 ACCGTTC 20 7.034228E-4 45.0 11 CGCGCAT 215 0.0 43.953487 36 ATATGCG 55 6.184564E-11 40.909092 1 ACTTTCG 365 0.0 40.68493 14 TACGCGC 240 0.0 39.375004 34 CTTTCGT 380 0.0 39.078945 15 ACGCGAG 75 0.0 39.0 1 ACGGGTA 185 0.0 38.91892 5 CGTAAGG 180 0.0 38.75 2 CGTTTTT 1415 0.0 38.639576 1 TACGATA 35 6.2500294E-6 38.57143 23 CTACGAT 35 6.2500294E-6 38.57143 22 CCCTCGT 340 0.0 38.382355 14 TTTCGTC 390 0.0 38.076923 16 TTCGTCT 385 0.0 37.987015 17 TTTAGCG 60 1.5643309E-10 37.500004 1 >>END_MODULE