##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935030.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1574566
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.304731589530064	33.0	31.0	34.0	30.0	34.0
2	32.47490165544029	34.0	31.0	34.0	31.0	34.0
3	32.48759785236059	34.0	31.0	34.0	31.0	34.0
4	36.00454664967997	37.0	35.0	37.0	35.0	37.0
5	35.92105189620505	37.0	35.0	37.0	35.0	37.0
6	35.86810524296854	37.0	35.0	37.0	35.0	37.0
7	36.125117651467136	37.0	35.0	37.0	35.0	37.0
8	35.88143018457149	37.0	35.0	37.0	35.0	37.0
9	37.62012834012674	39.0	38.0	39.0	35.0	39.0
10	37.45404447955818	39.0	37.0	39.0	35.0	39.0
11	37.37123880485162	39.0	37.0	39.0	35.0	39.0
12	37.210346851132314	39.0	37.0	39.0	34.0	39.0
13	37.113120694845435	39.0	37.0	39.0	33.0	39.0
14	38.193587947409	40.0	37.0	41.0	33.0	41.0
15	38.25358670262155	40.0	37.0	41.0	34.0	41.0
16	38.349510912848366	40.0	37.0	41.0	34.0	41.0
17	38.39492723709263	40.0	37.0	41.0	34.0	41.0
18	38.37453177574011	40.0	37.0	41.0	34.0	41.0
19	38.358573092522	40.0	37.0	41.0	34.0	41.0
20	38.329255172536435	40.0	37.0	41.0	34.0	41.0
21	38.25042646672162	40.0	37.0	41.0	34.0	41.0
22	38.20292448839871	40.0	37.0	41.0	34.0	41.0
23	38.0196193744816	40.0	36.0	41.0	34.0	41.0
24	37.8019917869432	40.0	36.0	41.0	33.0	41.0
25	37.76192233288411	40.0	36.0	41.0	33.0	41.0
26	37.93621226420487	40.0	36.0	41.0	34.0	41.0
27	37.95465290118039	40.0	36.0	41.0	34.0	41.0
28	37.93457308236047	40.0	36.0	41.0	34.0	41.0
29	37.98545885024826	40.0	36.0	41.0	34.0	41.0
30	37.90637737636911	40.0	36.0	41.0	34.0	41.0
31	37.79209191612165	40.0	36.0	41.0	34.0	41.0
32	37.69640904223767	40.0	36.0	41.0	33.0	41.0
33	37.61491801550395	40.0	36.0	41.0	33.0	41.0
34	37.43780508406761	40.0	35.0	41.0	33.0	41.0
35	37.37151507145461	40.0	35.0	41.0	33.0	41.0
36	37.32888300649195	39.0	35.0	41.0	33.0	41.0
37	37.224068727509675	39.0	35.0	41.0	33.0	41.0
38	37.14726216620961	39.0	35.0	41.0	33.0	41.0
39	37.111427529871726	39.0	35.0	41.0	33.0	41.0
40	37.04163941047882	39.0	35.0	41.0	32.0	41.0
41	36.993682703678346	39.0	35.0	41.0	32.0	41.0
42	36.95613902497577	39.0	35.0	41.0	32.0	41.0
43	36.893883139862034	39.0	35.0	41.0	32.0	41.0
44	36.76606442664201	39.0	35.0	41.0	32.0	41.0
45	36.76085600730614	39.0	35.0	41.0	32.0	41.0
46	36.66315988024637	39.0	35.0	41.0	32.0	41.0
47	36.6020598691957	39.0	35.0	41.0	32.0	41.0
48	36.54695135040386	39.0	35.0	40.0	31.0	41.0
49	36.499522408079436	38.0	35.0	40.0	31.0	41.0
50	36.4262990563749	38.0	35.0	40.0	31.0	41.0
51	35.415723443793404	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	5.0
9	23.0
10	34.0
11	27.0
12	29.0
13	37.0
14	43.0
15	77.0
16	87.0
17	215.0
18	361.0
19	679.0
20	1078.0
21	1768.0
22	2620.0
23	3893.0
24	5466.0
25	6980.0
26	8489.0
27	10429.0
28	12200.0
29	14783.0
30	18790.0
31	24869.0
32	34049.0
33	50108.0
34	99158.0
35	135099.0
36	118395.0
37	188026.0
38	331469.0
39	504880.0
40	398.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.73118179866706	21.91969088625056	25.444154135171214	10.904973179911163
2	30.285742229922402	24.906672695841266	26.169941431480165	18.637643642756164
3	31.556441584538213	24.759139978889422	27.042308801282385	16.642109635289977
4	28.8596349724305	25.649417045712912	26.58853296717953	18.90241501467706
5	25.048997628552883	30.247572982015363	24.988600033279013	19.714829356152745
6	27.049548891567582	32.749024175550595	24.049230073556778	16.152196859325045
7	79.93364520763183	6.187927339978128	8.636221028524686	5.24220642386537
8	79.5349956749987	4.842540738209767	8.600846201429473	7.021617385362061
9	73.89795029233453	7.028984494775068	10.82088651730064	8.25217869558977
10	42.99724495511779	23.488758172093135	18.52961387455337	14.984382998235704
11	30.365573751751274	23.729713457549572	24.194603465335845	21.710109325363305
12	27.03049602239601	20.55328261882957	30.81687271286183	21.59934864591259
13	24.504276098937737	24.089685665764407	31.78533005285266	19.620708182445195
14	20.14574174724972	27.69702889558139	30.678104315728906	21.47912504143999
15	17.09461527811473	25.452029321095466	36.14970728441996	21.303648116369843
16	21.05494466411697	25.335425761765464	31.153028834612208	22.45660073950536
17	21.8217591387087	24.055517520383393	28.168269859758183	25.954453481149724
18	22.03788218467819	24.466233870158508	31.53910347359209	21.956780471571214
19	23.98260854102019	25.440216542209093	27.796738910912595	22.780436005858125
20	24.566515471564863	26.638133936589508	28.07529185820093	20.7200587336447
21	23.203282682339132	25.677932839906358	29.935105927601636	21.18367855015287
22	21.428761957263145	24.987456861128717	27.821063073888297	25.76271810771984
23	21.502115503573684	25.135497654591806	29.17527750503948	24.18710933679503
24	22.946894572853726	23.92221094574632	28.235335959242104	24.895558522157852
25	20.856286748221414	26.704755469126095	27.293235088271945	25.145722694380545
26	20.49574295393143	26.571194856233397	28.551423058798424	24.381639131036742
27	21.12359850269852	26.720696369666307	29.92202295743716	22.23368217019801
28	18.075266454375363	26.260061502661685	30.28193165608809	25.382740386874858
29	21.712903746175137	24.043196664985782	30.221343532122503	24.02255605671658
30	21.91130762381507	24.720653183162852	28.79345800684125	24.574581186180826
31	21.52516947527128	27.968722809967954	25.400396045640512	25.10571166912025
32	24.76460180138527	27.013983535780657	24.97450091009205	23.246913752742028
33	23.69452915914608	25.876908303621445	25.388964324137568	25.03959821309491
34	20.90639579414264	24.91969215644184	28.20529593551493	25.968616113900595
35	21.91422906375471	25.41678151312806	28.37829598759277	24.290693435524453
36	24.96002072952166	24.798325379818948	27.942175812255567	22.299478078403826
37	22.480289806842013	27.037926641372923	28.29554302582426	22.186240525960805
38	22.705050153502615	28.539483260784245	24.452960371302314	24.30250621441083
39	23.982989598403623	23.19216850865572	27.198415309361433	25.62642658357922
40	23.11824337627003	22.548245040220607	29.309282684879516	25.024228898629847
41	20.070609933149832	25.289444837498078	27.47557104624385	27.164374183108237
42	22.628902186380248	24.25893865357184	29.39133704144507	23.72082211860284
43	24.369508804330845	23.13812186977237	28.921683816365906	23.570685509530882
44	21.93194823208427	23.30686678106856	29.052640537138487	25.708544449708683
45	22.144324213783353	22.756048333318514	26.915289673471925	28.184337779426205
46	23.016818602713382	24.3811310545255	28.20447031118416	24.397580031576954
47	19.8382284388206	24.37674889461604	31.14680489734949	24.638217769213867
48	21.248839362719632	23.462846270019803	29.17070481643831	26.11760955082226
49	22.86458617803255	21.88819014255357	31.03864811001889	24.208575569394995
50	21.241218215051006	22.013494512138582	29.881249817410005	26.864037455400407
51	20.87286274440068	22.696031795428073	26.317601167559822	30.113504292611427
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1334.0
1	1650.0
2	1966.0
3	2575.0
4	3184.0
5	2564.5
6	1945.0
7	2011.0
8	2077.0
9	2235.5
10	2394.0
11	2444.0
12	2494.0
13	2561.5
14	2629.0
15	2809.5
16	2990.0
17	2860.0
18	2730.0
19	2877.0
20	3024.0
21	3785.0
22	4546.0
23	5254.5
24	5963.0
25	6731.5
26	9469.0
27	11438.0
28	13216.0
29	14994.0
30	16312.5
31	17631.0
32	22150.0
33	26669.0
34	31099.0
35	35529.0
36	39716.5
37	43904.0
38	46787.5
39	49671.0
40	56932.0
41	64193.0
42	75228.5
43	86264.0
44	96439.0
45	106614.0
46	127049.0
47	147484.0
48	166321.5
49	185159.0
50	181559.0
51	177959.0
52	148708.0
53	119457.0
54	102491.5
55	85526.0
56	78632.5
57	71739.0
58	68287.0
59	64835.0
60	62072.5
61	59310.0
62	53420.0
63	47530.0
64	41909.0
65	36288.0
66	32442.5
67	28597.0
68	24078.0
69	19559.0
70	15669.5
71	11780.0
72	9701.0
73	7622.0
74	5987.0
75	3521.5
76	2691.0
77	1990.0
78	1289.0
79	1138.5
80	988.0
81	680.5
82	373.0
83	307.0
84	241.0
85	150.0
86	59.0
87	41.0
88	23.0
89	17.5
90	12.0
91	9.0
92	6.0
93	5.0
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1574566.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.731166774698938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.49841531161809	12.726289892173323
2	12.90550461310798	5.0928132766576
3	6.142369547634837	3.6358835380864507
4	3.619069338344804	2.8563384273632266
5	2.3201978083055073	2.289010495298345
6	1.5252619561446346	1.8057118819075995
7	1.1179638175453748	1.5441111372446814
8	0.823885382647028	1.3004975910596128
9	0.6408250838185531	1.1379803942010869
>10	4.100511547645161	16.356846807048097
>50	1.2297137564313965	18.102305404765545
>100	1.0626002879850287	29.184867339710273
>500	0.0074922767082437666	0.9306305563372492
>1k	0.005212018579647838	1.6490266975197503
>5k	9.772534836839696E-4	1.3876865606271989
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	8080	0.5131572763542462	No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	7804	0.49562863671640306	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGCT	5706	0.36238557164323376	No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	2971	0.18868691436243384	No Hit
ACTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC	2393	0.15197838642521178	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC	2168	0.13768873454653535	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTC	2153	0.13673609108795695	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCG	2026	0.12867037647199292	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCC	1658	0.10529885695486883	No Hit
CGCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTG	1588	0.10085318748150283	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.350956390522849E-5	0.0	0.0	0.37711979046924676	0.0
2	1.2701912781045699E-4	0.0	0.0	1.7842376883534892	0.0
3	1.2701912781045699E-4	0.0	0.0	2.4670290098986007	0.0
4	1.2701912781045699E-4	0.0	0.0	3.310753566379561	0.0
5	1.2701912781045699E-4	0.0	0.0	5.538669068174976	0.0
6	1.2701912781045699E-4	0.0	0.0	6.601882677512407	0.0
7	1.2701912781045699E-4	0.0	0.0	7.811422322087483	0.0
8	1.2701912781045699E-4	0.0	0.0	9.512843539108554	0.0
9	1.2701912781045699E-4	0.0	0.0	10.092495328871575	0.0
10	1.2701912781045699E-4	0.0	0.0	11.547435928376455	0.0
11	1.2701912781045699E-4	0.0	0.0	13.702315431680857	0.0
12	1.2701912781045699E-4	0.0	0.0	15.488712445207124	0.0
13	1.2701912781045699E-4	0.0	0.0	16.12526880422923	0.0
14	1.2701912781045699E-4	0.0	0.0	16.37562350514364	0.0
15	1.2701912781045699E-4	0.0	0.0	16.845975335425763	0.0
16	1.9052869171568547E-4	0.0	0.0	17.847775196466834	0.0
17	1.9052869171568547E-4	0.0	0.0	19.032546111118872	0.0
18	1.9052869171568547E-4	0.0	0.0	20.452873998295402	0.0
19	1.9052869171568547E-4	0.0	0.0	21.297614707798846	0.0
20	1.9052869171568547E-4	0.0	0.0	22.048043714903027	0.0
21	1.9052869171568547E-4	0.0	0.0	22.964359702927663	0.0
22	1.9052869171568547E-4	0.0	0.0	23.92087724490431	0.0
23	1.9052869171568547E-4	0.0	0.0	24.813123108208867	0.0
24	1.9052869171568547E-4	0.0	0.0	25.547992272156264	0.0
25	2.5403825562091397E-4	0.0	0.0	26.185628293764758	0.0
26	2.5403825562091397E-4	0.0	0.0	26.75486451504732	0.0
27	2.5403825562091397E-4	0.0	0.0	27.329117991878398	0.0
28	2.5403825562091397E-4	0.0	0.0	27.91975693619702	0.0
29	2.5403825562091397E-4	0.0	0.0	28.50582319191447	0.0
30	2.5403825562091397E-4	0.0	0.0	29.169561644288013	0.0
31	2.5403825562091397E-4	0.0	0.0	29.781793840334416	0.0
32	2.5403825562091397E-4	0.0	0.0	30.34594929650456	6.350956390522849E-5
33	3.8105738343137093E-4	0.0	0.0	30.945797127589444	6.350956390522849E-5
34	3.8105738343137093E-4	0.0	0.0	31.510841717654262	6.350956390522849E-5
35	3.8105738343137093E-4	0.0	0.0	32.12853573619651	6.350956390522849E-5
36	3.8105738343137093E-4	0.0	0.0	32.68824552289329	6.350956390522849E-5
37	3.8105738343137093E-4	0.0	0.0	33.27558196988885	6.350956390522849E-5
38	3.8105738343137093E-4	0.0	0.0	33.86850725850806	6.350956390522849E-5
39	3.8105738343137093E-4	0.0	0.0	34.6111880988158	6.350956390522849E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGTTT	30	2.1660162E-6	45.000004	13
ACCGCGT	30	2.1660162E-6	45.000004	4
ACGGGTA	260	0.0	45.000004	5
CGCTATA	30	2.1660162E-6	45.000004	6
CTCGCTA	30	2.1660162E-6	45.000004	37
TTACGTA	30	2.1660162E-6	45.000004	43
GCGTAGT	30	2.1660162E-6	45.000004	10
ACGACTT	30	2.1660162E-6	45.000004	34
TACGTAA	30	2.1660162E-6	45.000004	44
TCAAACG	30	2.1660162E-6	45.000004	1
ACTATCC	25	3.891465E-5	45.0	26
AATCCCG	20	7.034082E-4	45.0	42
TCGCACA	20	7.034082E-4	45.0	19
CGTATCG	20	7.034082E-4	45.0	30
CGTATAG	25	3.891465E-5	45.0	2
ATAGCCG	45	3.8562575E-10	45.0	1
ATTCGTC	40	6.8175723E-9	45.0	25
TAAGTCG	25	3.891465E-5	45.0	18
CGTTAGT	25	3.891465E-5	45.0	15
CCCGTTA	25	3.891465E-5	45.0	33
>>END_MODULE