##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935027.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620485
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2154975543325	33.0	31.0	34.0	30.0	34.0
2	32.39309572350661	34.0	31.0	34.0	30.0	34.0
3	32.398272319234145	34.0	31.0	34.0	30.0	34.0
4	35.943598958878944	37.0	35.0	37.0	35.0	37.0
5	35.84539029952376	37.0	35.0	37.0	35.0	37.0
6	35.80122323666164	37.0	35.0	37.0	35.0	37.0
7	36.072718921488836	37.0	35.0	37.0	35.0	37.0
8	35.82298202212785	37.0	35.0	37.0	35.0	37.0
9	37.58200601142654	39.0	38.0	39.0	35.0	39.0
10	37.40255928829867	39.0	37.0	39.0	35.0	39.0
11	37.30251335648727	39.0	37.0	39.0	34.0	39.0
12	37.05439132291675	39.0	35.0	39.0	34.0	39.0
13	36.84617355778141	39.0	35.0	39.0	33.0	39.0
14	37.92174992143243	40.0	36.0	41.0	33.0	41.0
15	38.04810914043047	40.0	36.0	41.0	33.0	41.0
16	38.137321611320175	40.0	36.0	41.0	34.0	41.0
17	38.18239119398535	40.0	36.0	41.0	34.0	41.0
18	38.1975196821841	40.0	36.0	41.0	34.0	41.0
19	38.15388607299129	40.0	36.0	41.0	34.0	41.0
20	38.103180576484526	40.0	36.0	41.0	34.0	41.0
21	38.02454531535815	40.0	36.0	41.0	34.0	41.0
22	37.94933801784088	40.0	35.0	41.0	34.0	41.0
23	37.757489705633496	39.0	35.0	41.0	34.0	41.0
24	37.56435369106425	39.0	35.0	41.0	33.0	41.0
25	37.492808045319386	39.0	35.0	41.0	33.0	41.0
26	37.61681426625946	39.0	35.0	41.0	33.0	41.0
27	37.695404401395685	39.0	35.0	41.0	34.0	41.0
28	37.699119237370766	39.0	36.0	41.0	34.0	41.0
29	37.74014037406223	40.0	36.0	41.0	34.0	41.0
30	37.67638701983126	40.0	36.0	41.0	34.0	41.0
31	37.57176563494686	39.0	35.0	41.0	33.0	41.0
32	37.44611553865121	39.0	35.0	41.0	33.0	41.0
33	37.337253922334945	39.0	35.0	41.0	33.0	41.0
34	37.254961844363685	39.0	35.0	41.0	33.0	41.0
35	37.181254986019	39.0	35.0	41.0	33.0	41.0
36	37.10024094055456	39.0	35.0	41.0	33.0	41.0
37	37.04154169722072	39.0	35.0	41.0	33.0	41.0
38	36.96955446142937	39.0	35.0	41.0	32.0	41.0
39	36.90703240207257	39.0	35.0	41.0	32.0	41.0
40	36.8277782702241	39.0	35.0	41.0	32.0	41.0
41	36.778745658638	39.0	35.0	41.0	32.0	41.0
42	36.74105578700533	39.0	35.0	41.0	32.0	41.0
43	36.635372329709824	39.0	35.0	41.0	32.0	41.0
44	36.50559159367269	39.0	35.0	41.0	31.0	41.0
45	36.53044473274938	39.0	35.0	41.0	31.0	41.0
46	36.420919119720864	38.0	35.0	40.0	31.0	41.0
47	36.35291264091799	38.0	35.0	40.0	31.0	41.0
48	36.34058679903624	38.0	35.0	40.0	31.0	41.0
49	36.2876459543744	38.0	35.0	40.0	31.0	41.0
50	36.19064925018333	38.0	35.0	40.0	31.0	41.0
51	35.169878401572966	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	10.0
11	4.0
12	7.0
13	11.0
14	16.0
15	34.0
16	53.0
17	98.0
18	150.0
19	286.0
20	541.0
21	880.0
22	1325.0
23	1912.0
24	2520.0
25	3172.0
26	3799.0
27	4402.0
28	5144.0
29	6255.0
30	8026.0
31	10570.0
32	14168.0
33	20562.0
34	41455.0
35	68767.0
36	42979.0
37	68361.0
38	127835.0
39	187014.0
40	120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.26345520036745	22.910142872108107	27.075916420219663	11.75048550730477
2	29.04228144113073	25.76178312126804	27.201141042893866	17.994794394707366
3	29.442129946735214	25.38836555275309	28.0382281602295	17.1312763402822
4	28.974592455901433	26.63432637372378	26.342619080235625	18.048462090139164
5	24.55031144991418	29.53173727003876	25.02203921126216	20.895912068784902
6	25.984995608274176	33.53554074635164	23.851664423797516	16.62779922157667
7	77.36061306880909	6.635776851978695	10.099680088962666	5.903929990249563
8	77.42781856128673	5.1906170173332145	9.783798157892615	7.597766263487434
9	71.84895686438834	7.03562535758318	12.846080082516096	8.269337695512382
10	41.41179883478247	25.503436827642894	18.71342578789173	14.37133854968291
11	30.966099099897658	23.258418817537894	25.64767883188151	20.127803250682934
12	28.984906967936375	20.838054102838907	30.634906565025744	19.542132364198974
13	21.47126844323392	29.3393071548869	30.972706834170044	18.216717567709132
14	16.66857377696479	32.38402217620088	30.345294406794686	20.602109640039647
15	14.182937540794702	25.63945945510367	41.10929353650773	19.0683094675939
16	15.94994238378043	26.30248918184968	34.86707978436223	22.880488650007656
17	16.835539940530392	24.57464725174662	28.57651675705295	30.01329605067004
18	19.515862591359983	25.681039831744524	31.717769164443943	23.08532841245155
19	20.572777746440284	27.418229288379255	28.03887281723168	23.97012014794878
20	23.17719203526274	27.86884453290571	28.30108705286993	20.65287637896162
21	20.46205790631522	28.572971143540936	29.219078623979627	21.74589232616421
22	17.58543719832067	26.627396310950306	26.87623391379324	28.91093257693578
23	17.228780711862495	28.043063087745878	28.89159286687027	25.836563333521354
24	22.38764837183816	23.80218699887991	30.50081790857152	23.309346720710412
25	16.736101597943545	28.175378937444094	28.502381201801818	26.586138262810543
26	17.59687986010943	32.229465659927314	27.75135579425772	22.422298685705535
27	20.95280304922762	30.2116892430921	27.271569820382442	21.56393788729784
28	16.597500342474035	26.63432637372378	31.671192696036165	25.096980587766023
29	17.561746053490417	26.56486458173848	31.1114692538901	24.761920110881004
30	23.461002280474144	28.133637396552697	26.20675761702539	22.198602705947767
31	25.15677252471857	28.02614084143855	22.998460881407286	23.818625752435597
32	25.68716407326527	29.149294503493234	23.20088318009299	21.962658243148507
33	24.30082919006906	29.607000975043718	22.91449430687285	23.177675528014376
34	21.11622359928121	26.94779084103564	27.198401250634586	24.737584309048565
35	22.72254768447263	27.308798762258558	26.113604680209836	23.85504887305898
36	25.968234526217394	28.65290861181173	24.402362667913003	20.976494194057874
37	22.793137626211752	26.635776851978694	28.78280699775176	21.788278524057795
38	20.317332409324965	28.833573736673728	25.796111106634328	25.052982747366983
39	22.638258781437102	28.44258926484927	28.077713401613252	20.84143855210037
40	26.523767697849266	25.18997236033103	26.515387156820875	21.770872784998833
41	22.95107859174678	26.258330177200094	24.99012868965406	25.800462541399067
42	24.719211584486327	26.13407254002917	25.354359895887896	23.792355979596604
43	24.91534847740074	24.832187724119038	27.604051669258723	22.648412129221494
44	20.617581408092057	25.697962078051845	28.15442758487312	25.530028928982972
45	22.39812404812365	24.982231641377307	25.662344778681195	26.957299531817853
46	25.85396907258032	25.358389002151544	26.67091065859771	22.116731266670428
47	21.438552100373094	25.12325036060501	30.814927032885564	22.62327050613633
48	22.568313496700164	21.469012143726278	30.352224469568164	25.6104498900054
49	21.212438656857135	21.50527410009912	33.16083386383232	24.121453379211424
50	20.580674794717034	22.427133613221915	31.23959483307413	25.752596758986922
51	20.134572149205862	23.313536991224606	26.395642118665236	30.15624874090429
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	921.0
1	1013.5
2	1106.0
3	1205.0
4	1304.0
5	1097.0
6	890.0
7	970.5
8	1051.0
9	1101.0
10	1151.0
11	1267.0
12	1383.0
13	1463.0
14	1543.0
15	1513.0
16	1483.0
17	1662.5
18	1842.0
19	1838.5
20	1835.0
21	2006.5
22	2178.0
23	2231.5
24	2285.0
25	2236.0
26	2922.0
27	3657.0
28	4398.0
29	5139.0
30	7225.0
31	9311.0
32	9611.5
33	9912.0
34	11794.5
35	13677.0
36	13995.0
37	14313.0
38	16815.5
39	19318.0
40	22728.0
41	26138.0
42	33612.5
43	41087.0
44	46630.0
45	52173.0
46	61601.0
47	71029.0
48	76702.0
49	82375.0
50	80143.5
51	77912.0
52	62975.5
53	48039.0
54	39856.0
55	31673.0
56	26777.5
57	21882.0
58	20062.0
59	18242.0
60	17602.5
61	16963.0
62	14436.0
63	11909.0
64	10022.0
65	8135.0
66	7264.5
67	6394.0
68	5317.0
69	4240.0
70	3350.5
71	2461.0
72	1933.0
73	1405.0
74	1094.5
75	655.5
76	527.0
77	461.5
78	396.0
79	275.0
80	154.0
81	99.5
82	45.0
83	35.0
84	25.0
85	13.0
86	1.0
87	3.5
88	6.0
89	4.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	620485.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.815883728605336
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.5144819755533	14.94770803294034
2	12.752499621230207	5.819190972141439
3	6.109328739935204	4.181692025705658
4	3.6103889777482987	3.294968605253738
5	2.2591280747325073	2.577200174056246
6	1.5308355241480451	2.0956419195948244
7	1.0912573292085175	1.7428600238846952
8	0.8094386646341983	1.477444676618515
9	0.586183172986615	1.2036858406674802
>10	3.8117507111964732	17.720404420709936
>50	1.2973794058889134	21.692457292543704
>100	0.6137985992734596	18.872902490248233
>500	0.008544760082693174	1.3966990858734758
>1k	0.004272380041346587	2.095779714757222
>5k	7.120633402244311E-4	0.8813647250044907
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	5425	0.8743160592117457	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	4316	0.6955849053562939	No Hit
CTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGCT	3190	0.5141139592415611	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	1945	0.3134644673118609	No Hit
ACTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	1389	0.22385714400831608	No Hit
CGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTG	1032	0.1663215065634141	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGC	1028	0.16567684956123033	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCG	956	0.15407302352192237	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTC	903	0.14553131824298735	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCC	819	0.13199352119712807	No Hit
ACCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTG	786	0.1266751009291119	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	762	0.12280715891600925	No Hit
GCCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTG	733	0.11813339565017687	No Hit
AAAACTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTC	686	0.11055867587451752	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTC	667	0.10749655511414459	No Hit
GGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTG	649	0.10459559860431758	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5389332538256364	0.0
2	0.0	0.0	0.0	2.752201906573084	0.0
3	0.0	0.0	0.0	3.6993642070315964	0.0
4	0.0	0.0	0.0	4.824935332844468	0.0
5	0.0	0.0	0.0	7.776497417342885	0.0
6	0.0	0.0	0.0	9.26275413587758	0.0
7	0.0	0.0	0.0	10.902922713683651	0.0
8	0.0	0.011764990289853904	0.0	13.195000684948065	0.0
9	0.0	0.011764990289853904	0.0	13.993730710653763	0.0
10	0.0	0.011764990289853904	0.0	15.875001007276566	0.0
11	0.0	0.011764990289853904	0.0	18.619789358324535	0.0
12	0.0	0.011764990289853904	0.0	20.82419397729196	0.0
13	0.0	0.011764990289853904	0.0	21.630015230021677	0.0
14	0.0	0.011764990289853904	0.0	21.92784676503058	0.0
15	0.0	0.011764990289853904	0.0	22.500624511470864	0.0
16	0.0	0.011764990289853904	0.0	23.7667308637598	0.0
17	0.0	0.011764990289853904	0.0	25.245412862518837	0.0
18	0.0	0.011764990289853904	0.0	27.049646647380676	0.0
19	1.611642505459439E-4	0.011764990289853904	0.0	28.05128246452372	0.0
20	1.611642505459439E-4	0.011764990289853904	0.0	28.89368800212737	0.0
21	1.611642505459439E-4	0.011764990289853904	0.0	29.934486732153072	0.0
22	1.611642505459439E-4	0.011764990289853904	0.0	30.96126417238128	0.0
23	1.611642505459439E-4	0.011764990289853904	0.0	32.05685874759261	0.0
24	1.611642505459439E-4	0.011764990289853904	0.0	32.7924123870843	0.0
25	1.611642505459439E-4	0.011764990289853904	0.0	33.47687695915292	0.0
26	1.611642505459439E-4	0.011764990289853904	0.0	34.10412822227774	0.0
27	1.611642505459439E-4	0.011764990289853904	0.0	34.65708276590087	0.0
28	1.611642505459439E-4	0.011764990289853904	0.0	35.282077729518036	0.0
29	1.611642505459439E-4	0.011764990289853904	0.0	35.93092500221601	0.0
30	1.611642505459439E-4	0.011764990289853904	0.0	36.671313569224075	0.0
31	1.611642505459439E-4	0.011764990289853904	0.0	37.35287718478287	0.0
32	1.611642505459439E-4	0.011764990289853904	0.0	37.879884284068105	0.0
33	1.611642505459439E-4	0.011764990289853904	0.0	38.4446038179811	0.0
34	1.611642505459439E-4	0.011764990289853904	0.0	38.97322255977179	0.0
35	1.611642505459439E-4	0.011764990289853904	0.0	39.59853985189005	0.0
36	1.611642505459439E-4	0.011764990289853904	0.0	40.13666728446296	0.0
37	1.611642505459439E-4	0.011764990289853904	0.0	40.66174041274164	0.0
38	1.611642505459439E-4	0.011764990289853904	0.0	41.18326792750832	0.0
39	1.611642505459439E-4	0.011764990289853904	0.0	41.70495660652554	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCTA	20	7.0307753E-4	45.0	13
ATTTACC	20	7.0307753E-4	45.0	34
CGTGGTT	20	7.0307753E-4	45.0	18
AACGTAG	20	7.0307753E-4	45.0	1
AGCCGTA	20	7.0307753E-4	45.0	13
GGAATCG	20	7.0307753E-4	45.0	8
ACTATCG	25	3.888723E-5	45.0	1
AATCCCG	25	3.888723E-5	45.0	10
GTCGCGA	40	6.8066583E-9	45.0	16
TACCCGT	25	3.888723E-5	45.0	41
CAAACGA	20	7.0307753E-4	45.0	6
TACCCAC	25	3.888723E-5	45.0	31
ATCTACG	35	1.2107193E-7	45.0	2
GGTCGAT	25	3.888723E-5	45.0	16
TTTACGT	25	3.888723E-5	45.0	15
CGCGGAA	20	7.0307753E-4	45.0	25
CGTGCCG	20	7.0307753E-4	45.0	36
CGTGCAT	25	3.888723E-5	45.0	39
CCCTAGT	35	1.2107193E-7	45.0	12
ACGCACT	20	7.0307753E-4	45.0	31
>>END_MODULE