##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935026.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1778075
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33044500372594	33.0	31.0	34.0	30.0	34.0
2	32.50276281934114	34.0	31.0	34.0	31.0	34.0
3	32.51004935112411	34.0	31.0	34.0	31.0	34.0
4	36.01458431168539	37.0	35.0	37.0	35.0	37.0
5	35.93864881965047	37.0	35.0	37.0	35.0	37.0
6	35.86976364888995	37.0	35.0	37.0	35.0	37.0
7	36.11934817147758	37.0	35.0	37.0	35.0	37.0
8	35.789552746650166	37.0	35.0	37.0	35.0	37.0
9	37.53657297920504	39.0	38.0	39.0	35.0	39.0
10	37.41736259719078	39.0	37.0	39.0	35.0	39.0
11	37.37311193284873	39.0	37.0	39.0	35.0	39.0
12	37.201781139715706	39.0	37.0	39.0	34.0	39.0
13	37.084340649297694	39.0	37.0	39.0	33.0	39.0
14	38.246521659660026	40.0	37.0	41.0	33.0	41.0
15	38.303806644826565	40.0	37.0	41.0	34.0	41.0
16	38.39637248147575	40.0	37.0	41.0	34.0	41.0
17	38.38585042813155	40.0	37.0	41.0	34.0	41.0
18	38.40529842666929	40.0	37.0	41.0	34.0	41.0
19	38.39115391645459	40.0	37.0	41.0	34.0	41.0
20	38.32830504899962	40.0	37.0	41.0	34.0	41.0
21	38.28929038426388	40.0	37.0	41.0	34.0	41.0
22	38.222771255430736	40.0	37.0	41.0	34.0	41.0
23	38.007815193397356	40.0	36.0	41.0	34.0	41.0
24	37.773930795945056	40.0	36.0	41.0	33.0	41.0
25	37.728816838434824	40.0	36.0	41.0	33.0	41.0
26	37.891077148039315	40.0	36.0	41.0	34.0	41.0
27	37.90655905965721	40.0	36.0	41.0	34.0	41.0
28	37.84868860987304	40.0	36.0	41.0	33.0	41.0
29	37.90495845225876	40.0	36.0	41.0	34.0	41.0
30	37.83950958199176	40.0	36.0	41.0	34.0	41.0
31	37.76080592775895	40.0	36.0	41.0	33.0	41.0
32	37.659850118808265	40.0	36.0	41.0	33.0	41.0
33	37.57102259465996	40.0	36.0	41.0	33.0	41.0
34	37.477241398703654	40.0	36.0	41.0	33.0	41.0
35	37.39272359152454	40.0	35.0	41.0	33.0	41.0
36	37.32718361148996	40.0	35.0	41.0	33.0	41.0
37	37.28883877226776	40.0	35.0	41.0	33.0	41.0
38	37.20230586448828	39.0	35.0	41.0	32.0	41.0
39	37.164446944026544	39.0	35.0	41.0	32.0	41.0
40	37.08751318138999	39.0	35.0	41.0	32.0	41.0
41	36.98633803410992	39.0	35.0	41.0	32.0	41.0
42	36.97069921122562	39.0	35.0	41.0	32.0	41.0
43	36.91608846645951	39.0	35.0	41.0	32.0	41.0
44	36.82182022693081	39.0	35.0	41.0	32.0	41.0
45	36.80524556050785	39.0	35.0	41.0	32.0	41.0
46	36.67584438226734	39.0	35.0	41.0	31.0	41.0
47	36.57660447393951	39.0	35.0	41.0	31.0	41.0
48	36.52561899807376	39.0	35.0	41.0	31.0	41.0
49	36.45658928897825	39.0	35.0	40.0	31.0	41.0
50	36.39556261687499	38.0	35.0	40.0	31.0	41.0
51	35.432310785540544	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	26.0
10	25.0
11	38.0
12	32.0
13	37.0
14	36.0
15	66.0
16	124.0
17	204.0
18	436.0
19	754.0
20	1281.0
21	2130.0
22	3315.0
23	4828.0
24	6618.0
25	8140.0
26	10443.0
27	12442.0
28	14545.0
29	17888.0
30	22474.0
31	29037.0
32	38652.0
33	55782.0
34	107300.0
35	152985.0
36	126896.0
37	199655.0
38	367317.0
39	594073.0
40	490.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.98553210635097	21.76876678430325	24.18176961039326	11.063931498952519
2	31.008422029441952	26.965341731929193	23.514981089099166	18.51125514952969
3	31.294292985391504	26.260084642098896	25.38976139926606	17.055860973243536
4	28.75486129662697	25.457418837788058	26.492639511831616	19.295080353753356
5	27.25436216132615	29.378119595630103	23.335179899610534	20.032338343433207
6	26.873894520759812	35.437087861873096	22.761525807404073	14.927491809963023
7	79.85146858259635	6.127300591932286	9.145058560521912	4.876172264949454
8	79.48044936237223	6.620699351827117	7.925031283832235	5.973820001968421
9	73.49105071495859	8.15910464969138	9.976519550637628	8.373325084712398
10	40.07266285167949	29.475359588318828	16.0681073633002	14.38387019670149
11	33.82933790756858	25.073239317801555	22.00233398478692	19.095088789842947
12	31.53922078652475	21.429748463928686	27.0273188701264	20.00371187942016
13	25.52457011093458	24.894900383842074	28.85097647737019	20.729553027853157
14	20.133177734347537	29.75881219858555	26.778622948975716	23.329387118091194
15	18.604614541006427	26.270151709011145	34.45861395048016	20.66661979950227
16	21.138422170043448	25.571980934437523	31.007016014510075	22.282580881008958
17	20.963795115504126	24.820381592452513	28.417024028795186	25.798799263248174
18	22.44961545491613	25.55848319109149	29.02442247936673	22.967478874625648
19	24.49733560170409	27.231978403610647	25.05411751472801	23.216568479957257
20	27.04829661291003	26.93654654612432	26.41418387863279	19.60097296233286
21	24.60981679625438	27.311671329949522	26.98446353500274	21.094048338793357
22	22.78475317407871	25.351461552521688	26.423744780169567	25.440040493230036
23	22.82828339636967	27.729257764717463	26.322286742685208	23.120172096227662
24	23.293168173446002	24.39705861676251	27.942522109584804	24.367251100206687
25	21.901157150288935	27.959619251156447	25.1699731451148	24.969250453439816
26	20.96970037821802	28.28812057984056	25.981637444989662	24.76054159695176
27	23.761652348747944	27.05386443204027	26.198894872263544	22.985588346948244
28	19.796015353683057	27.449966958649103	28.538391237714944	24.2156264499529
29	22.049744808289866	24.632875441137184	26.874007001954357	26.443372748618586
30	23.579039129395554	25.65066153002545	26.041589921684967	24.72870941889403
31	23.599004541428233	26.030229321035392	25.18465194100361	25.186114196532767
32	24.166809611518076	27.73791881669783	23.118878562490334	24.97639300929376
33	23.692757617085896	26.955274665016944	24.059952476695305	25.29201524120186
34	21.250172236829155	25.86415083728189	27.642141079538263	25.24353584635069
35	21.835636854463395	26.025786313850652	25.10793976632032	27.03063706536564
36	21.842441966733688	28.042461650942734	25.286222459682524	24.82887392264106
37	22.090744203703444	27.368811776781072	28.13632720779495	22.404116811720538
38	20.447562673115588	27.939653839123775	25.629402584255445	25.983380903505193
39	22.79538264696371	24.72398520872292	26.613388074181348	25.867244070132024
40	23.01888278053513	24.179182542918607	28.861887153241568	23.9400475233047
41	19.735500471015	27.73403821548585	27.25087524429509	25.279586069204058
42	22.166556528830338	27.14418683126415	26.903252112537434	23.786004527368082
43	23.419934479704175	25.593352361402076	26.191696075812327	24.795017083081422
44	21.792331594561535	24.805983999550072	26.638583861760612	26.763100544127777
45	22.29619110554954	23.171576002137144	26.163294574188377	28.368938318124936
46	23.112860818581893	25.46309913811285	26.25519171013596	25.168848333169297
47	20.376530798757084	25.62057281048325	29.65679175512844	24.34610463563123
48	20.963682634309578	24.29306975240077	28.253645093710894	26.48960251957876
49	21.27463689664384	23.728751599341987	29.905712638668223	25.090898865345952
50	20.162029160749686	24.208146450515304	28.786862196476527	26.84296219225848
51	19.98391518917931	23.95888812339187	25.660784837534973	30.396411849893845
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	805.0
1	1425.0
2	2045.0
3	2816.0
4	3587.0
5	2849.5
6	2112.0
7	2150.0
8	2188.0
9	2315.5
10	2443.0
11	2466.5
12	2490.0
13	2631.5
14	2773.0
15	2958.5
16	3144.0
17	3268.0
18	3392.0
19	3499.0
20	3606.0
21	3860.0
22	4114.0
23	4611.5
24	5109.0
25	8011.0
26	12253.5
27	13594.0
28	17821.5
29	22049.0
30	23372.5
31	24696.0
32	27380.5
33	30065.0
34	33660.5
35	37256.0
36	41869.5
37	46483.0
38	52694.0
39	58905.0
40	64431.0
41	69957.0
42	80061.5
43	90166.0
44	100409.5
45	110653.0
46	138838.0
47	167023.0
48	174728.5
49	182434.0
50	178041.0
51	173648.0
52	152844.5
53	132041.0
54	118272.5
55	104504.0
56	96920.5
57	89337.0
58	88177.5
59	87018.0
60	86812.5
61	86607.0
62	77986.0
63	69365.0
64	56951.0
65	44537.0
66	37646.5
67	30756.0
68	25514.5
69	20273.0
70	17480.5
71	14688.0
72	12373.5
73	10059.0
74	8206.5
75	4716.0
76	3078.0
77	2436.5
78	1795.0
79	1522.5
80	1250.0
81	843.0
82	436.0
83	270.0
84	104.0
85	120.0
86	136.0
87	94.5
88	53.0
89	34.0
90	15.0
91	12.5
92	10.0
93	7.0
94	4.0
95	4.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1778075.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.585563218642736
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.1070938934047	13.143302298686713
2	12.351545157246049	4.83823937050326
3	5.662178502294059	3.3269086503576037
4	3.1967637853822897	2.5044167685468994
5	1.9692368215075953	1.9284306130058042
6	1.3421976335085792	1.577261796179697
7	0.9706347815307442	1.3307300213118707
8	0.6916700039364959	1.0837397270829687
9	0.5381556370905167	0.9486073126534745
>10	3.6352031087946184	15.234094805010365
>50	1.4426615252417805	20.9231771149209
>100	1.0761037084097784	28.665596966364642
>500	0.010746514757056626	1.3848218894310669
>1k	0.004647141516565027	1.5960948313201149
>5k	0.0011617853791412568	1.5145778346246368
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	7205	0.40521350336740575	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGC	7057	0.39688989497068455	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCG	6192	0.3482417783276859	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTC	6171	0.3470607257849078	No Hit
CCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	4886	0.27479155828634905	No Hit
CTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGCT	3590	0.2019037442177636	No Hit
TCTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	2277	0.12805983999550075	No Hit
ACTGTCTCTTATACACATCTGACGCCGTCCTAATCGTATGCCGTCTTCTGC	1831	0.10297653361078694	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.6240597275143064E-5	0.0	0.0	0.21270193889459105	0.0
2	5.6240597275143064E-5	0.0	0.0	1.1693544985447746	0.0
3	5.6240597275143064E-5	0.0	0.0	1.6774882949256922	0.0
4	5.6240597275143064E-5	0.0	0.0	2.343433207260661	0.0
5	5.6240597275143064E-5	0.0	0.0	4.308929600832361	0.0
6	5.6240597275143064E-5	0.0	0.0	5.2025926915343845	0.0
7	5.6240597275143064E-5	0.0	0.0	6.158345401628165	0.0
8	5.6240597275143064E-5	0.0	0.0	7.5526622892735125	0.0
9	5.6240597275143064E-5	0.0	0.0	8.018390675308972	0.0
10	5.6240597275143064E-5	0.0	0.0	9.477440490418008	0.0
11	5.6240597275143064E-5	0.0	0.0	11.167245476146956	0.0
12	5.6240597275143064E-5	0.0	0.0	12.88410218916525	0.0
13	5.6240597275143064E-5	0.0	0.0	13.427217637051305	0.0
14	5.6240597275143064E-5	0.0	0.0	13.654317168848333	0.0
15	5.6240597275143064E-5	0.0	0.0	14.107278939302335	0.0
16	1.1248119455028613E-4	0.0	0.0	14.925523389058391	0.0
17	1.1248119455028613E-4	0.0	0.0	15.868846927154367	0.0
18	1.1248119455028613E-4	0.0	0.0	16.90991662331454	0.0
19	1.1248119455028613E-4	0.0	0.0	17.638063636235817	0.0
20	1.6872179182542919E-4	0.0	0.0	18.29101697060023	0.0
21	1.6872179182542919E-4	0.0	0.0	19.05662022130675	0.0
22	1.6872179182542919E-4	0.0	0.0	19.87773294152384	0.0
23	1.6872179182542919E-4	0.0	0.0	20.63709348593282	0.0
24	1.6872179182542919E-4	0.0	0.0	21.26366998017519	0.0
25	1.6872179182542919E-4	0.0	0.0	21.802173699084683	0.0
26	2.2496238910057226E-4	0.0	0.0	22.321724336712457	0.0
27	2.812029863757153E-4	0.0	0.0	22.84588670331679	0.0
28	2.812029863757153E-4	0.0	0.0	23.36324395765083	0.0
29	2.812029863757153E-4	0.0	0.0	23.891680609648073	0.0
30	3.3744358365085837E-4	0.0	0.0	24.487212294194563	0.0
31	3.9368418092600144E-4	0.0	0.0	25.012555713341676	0.0
32	3.9368418092600144E-4	0.0	0.0	25.563994769624454	0.0
33	3.9368418092600144E-4	0.0	0.0	26.04817007156616	0.0
34	4.499247782011445E-4	0.0	0.0	26.551467176581415	0.0
35	4.499247782011445E-4	0.0	0.0	27.070005483458235	0.0
36	4.499247782011445E-4	0.0	0.0	27.588487549737778	0.0
37	5.061653754762876E-4	0.0	0.0	28.11208751036936	0.0
38	5.061653754762876E-4	0.0	0.0	28.623033336614036	0.0
39	5.061653754762876E-4	0.0	0.0	29.13409164405326	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAG	30	2.1661745E-6	45.000004	2
TATTACG	30	2.1661745E-6	45.000004	1
CTTCGTA	20	7.0343295E-4	45.0	10
CACGATT	25	3.8916696E-5	45.0	16
CGTTACG	20	7.0343295E-4	45.0	43
ACGATTG	95	0.0	45.0	1
CACTCGA	20	7.0343295E-4	45.0	32
CCGTAAA	20	7.0343295E-4	45.0	25
TTAAGCG	50	2.1827873E-11	45.0	1
ACGGATA	25	3.8916696E-5	45.0	20
AACGCAT	25	3.8916696E-5	45.0	45
GTATACG	40	6.8193913E-9	45.0	1
GCTCGAA	25	3.8916696E-5	45.0	24
ATCGCGT	20	7.0343295E-4	45.0	24
TACCGTT	20	7.0343295E-4	45.0	29
TCTAGCG	90	0.0	42.5	1
AACACGT	225	0.0	42.0	41
CGGTACG	65	0.0	41.538464	2
TATACGG	65	0.0	41.538464	2
ACGTTAG	60	3.6379788E-12	41.250004	1
>>END_MODULE