SRR2935025.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935025.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	840588
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	4304	0.5120225366053287	TruSeq Adapter, Index 22 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	4101	0.48787277477194535	TruSeq Adapter, Index 22 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGCT	3206	0.3813996868858466	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	1673	0.19902734752340032	TruSeq Adapter, Index 22 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	1260	0.149895073448586	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCC	939	0.1117075190223986	No Hit
ACCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTG	930	0.11063683992633727	No Hit
GCCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTG	927	0.11027994689431683	TruSeq Adapter, Index 22 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTG	918	0.10920926779825552	No Hit

Adapter graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.0322026E-4	45.000004	25
CTATGCG	20	7.0322026E-4	45.000004	1
GCCTTCG	20	7.0322026E-4	45.000004	30
TACCCGT	20	7.0322026E-4	45.000004	35
CGTATCG	20	7.0322026E-4	45.000004	13
CGACGGT	20	7.0322026E-4	45.000004	28
TTAGCCG	20	7.0322026E-4	45.000004	30
CGCATGT	20	7.0322026E-4	45.000004	38
TATATCG	20	7.0322026E-4	45.000004	29
GTCTACG	20	7.0322026E-4	45.000004	38
AACGGTC	40	6.8121153E-9	45.000004	15
TAGCACG	65	0.0	45.000004	1
ATATCGA	20	7.0322026E-4	45.000004	30
TGCGCAC	20	7.0322026E-4	45.000004	10
CTACGCA	20	7.0322026E-4	45.000004	40
TCGCTCA	20	7.0322026E-4	45.000004	19
AGCGCGA	20	7.0322026E-4	45.000004	40
TGTTACG	20	7.0322026E-4	45.000004	1
AACGAAG	40	6.8121153E-9	45.000004	1
TCGTATC	20	7.0322026E-4	45.000004	12