##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935025.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	840588
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.289770969844916	33.0	31.0	34.0	30.0	34.0
2	32.46803071183505	34.0	31.0	34.0	31.0	34.0
3	32.479677321113314	34.0	31.0	34.0	31.0	34.0
4	36.00640860921165	37.0	35.0	37.0	35.0	37.0
5	35.91201040224224	37.0	35.0	37.0	35.0	37.0
6	35.85073186864433	37.0	35.0	37.0	35.0	37.0
7	36.110871199684034	37.0	35.0	37.0	35.0	37.0
8	35.84440415518661	37.0	35.0	37.0	35.0	37.0
9	37.586328855515426	39.0	38.0	39.0	35.0	39.0
10	37.445082489876135	39.0	37.0	39.0	35.0	39.0
11	37.363376588768816	39.0	37.0	39.0	35.0	39.0
12	37.14752768300285	39.0	37.0	39.0	34.0	39.0
13	36.98946689698164	39.0	37.0	39.0	33.0	39.0
14	38.01513107491423	40.0	37.0	41.0	33.0	41.0
15	38.14518051649559	40.0	37.0	41.0	33.0	41.0
16	38.248821063350896	40.0	37.0	41.0	34.0	41.0
17	38.30430603339567	40.0	37.0	41.0	34.0	41.0
18	38.30628678972339	40.0	37.0	41.0	34.0	41.0
19	38.28928559532137	40.0	37.0	41.0	34.0	41.0
20	38.248747305457606	40.0	37.0	41.0	34.0	41.0
21	38.163465336169445	40.0	36.0	41.0	34.0	41.0
22	38.09302059986581	40.0	36.0	41.0	34.0	41.0
23	37.91477513359696	40.0	36.0	41.0	34.0	41.0
24	37.70801867264343	40.0	35.0	41.0	33.0	41.0
25	37.649433491793836	39.0	35.0	41.0	33.0	41.0
26	37.764905042660615	40.0	35.0	41.0	34.0	41.0
27	37.810128148391364	40.0	36.0	41.0	34.0	41.0
28	37.75735318610306	40.0	36.0	41.0	34.0	41.0
29	37.76981589078122	40.0	36.0	41.0	34.0	41.0
30	37.73460720352896	40.0	36.0	41.0	34.0	41.0
31	37.58284438987947	40.0	35.0	41.0	33.0	41.0
32	37.48534954103556	39.0	35.0	41.0	33.0	41.0
33	37.45738578233332	39.0	35.0	41.0	33.0	41.0
34	37.33014865784427	39.0	35.0	41.0	33.0	41.0
35	37.272067885813264	39.0	35.0	41.0	33.0	41.0
36	37.203434976468856	39.0	35.0	41.0	33.0	41.0
37	37.12563586441872	39.0	35.0	41.0	33.0	41.0
38	37.05973080748238	39.0	35.0	41.0	32.0	41.0
39	37.020009802661946	39.0	35.0	41.0	32.0	41.0
40	36.96060733676902	39.0	35.0	41.0	32.0	41.0
41	36.90100620042161	39.0	35.0	41.0	32.0	41.0
42	36.8967484665496	39.0	35.0	41.0	32.0	41.0
43	36.82825712477456	39.0	35.0	41.0	32.0	41.0
44	36.702623639642724	39.0	35.0	41.0	32.0	41.0
45	36.68133140135239	39.0	35.0	41.0	32.0	41.0
46	36.587555377902135	39.0	35.0	40.0	31.0	41.0
47	36.53264500563891	38.0	35.0	40.0	31.0	41.0
48	36.50202953170876	38.0	35.0	40.0	31.0	41.0
49	36.45098669026919	38.0	35.0	40.0	31.0	41.0
50	36.374696046101064	38.0	35.0	40.0	31.0	41.0
51	35.34128015151299	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	16.0
10	16.0
11	14.0
12	12.0
13	16.0
14	14.0
15	29.0
16	45.0
17	114.0
18	179.0
19	368.0
20	566.0
21	1004.0
22	1458.0
23	2169.0
24	2915.0
25	3755.0
26	4664.0
27	5556.0
28	6821.0
29	8257.0
30	10541.0
31	13780.0
32	18927.0
33	28099.0
34	56825.0
35	80690.0
36	60098.0
37	96801.0
38	177121.0
39	259509.0
40	205.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.395427962331134	23.18246275226389	23.975122176381294	11.446987109023683
2	31.67960998729461	24.646794862643766	24.977158845950694	18.696436304110932
3	31.649868901292905	24.600755661513134	25.857613955945126	17.891761481248842
4	27.96911209772207	26.29064416812993	25.229125326557124	20.511118407590878
5	25.543191194735115	29.45057507363893	24.837375741742683	20.16885798988327
6	27.573912546931435	33.10884761619248	23.615611928792703	15.70162790808339
7	80.07775509524285	5.955236096637116	8.64418716422314	5.322821643896891
8	78.99993813854111	5.060505265361866	9.056398616206751	6.883157979890267
9	73.40706743374875	7.37709793620656	11.37727400343569	7.838560626608992
10	41.47227892855953	25.570909886888703	17.699990958709854	15.25682022584191
11	32.217566750893425	23.51675255892304	23.593841453839456	20.671839236344084
12	28.579399182477026	21.501853464479627	29.65519374533065	20.263553607712698
13	24.842253279846965	25.70831370421657	30.944648270020508	18.504784745915952
14	19.335155867083518	29.343507164032793	30.636768547730874	20.684568421152814
15	17.396869810180494	24.951105654613198	36.92236862767491	20.729655907531395
16	19.760334432563873	26.19380719210838	31.946447010901892	22.099411364425855
17	20.439144979466754	24.363778688251557	27.730826516676423	27.466249815605266
18	21.70789970829943	24.881154620337192	30.879574773848784	22.531370897514595
19	23.149152735941982	26.454458069827314	27.26936382627399	23.127025367956715
20	24.764331634522502	27.609482885789472	27.467439459045334	20.158746020642692
21	23.06825698201735	26.408180940008663	29.28652324325353	21.237038834720458
22	21.35564628569525	25.679762261654936	27.82088252508958	25.143708927560233
23	19.97351853702408	26.3652348118222	29.437488995798176	24.223757655355538
24	22.296892175477165	24.454905375760777	28.83505355774767	24.413148891014387
25	20.651496333518917	26.586389527330866	27.813387771417155	24.948726367733062
26	19.9214121543491	29.413815091340823	27.08401737831137	23.580755375998706
27	22.61797693995156	26.7248640237548	28.370378830057057	22.286780206236585
28	18.526793149557214	27.31195306142843	30.167097317591974	23.994156471422386
29	20.46293784826812	27.894402489685792	28.735242473125954	22.90741718892014
30	25.347613813187913	25.376165255749545	27.218803980071094	22.05741695099145
31	22.650216277177403	27.766991677254495	27.00906984158708	22.573722203981024
32	23.91445036093782	29.022065506526385	24.386262949268843	22.67722118326695
33	25.106592052230102	25.648117746149126	25.2374528306376	24.007837370983168
34	20.319109956363878	26.214863880997584	29.940232313570974	23.525793849067558
35	20.91357478336593	27.18037849695689	25.776123380300458	26.12992333937672
36	24.891861411297807	25.361413677092703	26.263996155072398	23.482728756537092
37	22.004120924876396	26.176200469195372	29.58952542743889	22.230153178489342
38	21.14032082304292	27.535724992505244	25.4399301441372	25.884024040314635
39	23.395170999348075	24.35104950344283	27.76782442766254	24.485955069546556
40	24.423974646319007	22.677102218922947	29.228944500754235	23.669978634003815
41	20.29448433715447	24.292994903567504	27.397250496081316	28.01527026319671
42	22.210761990416234	23.357221373609903	28.077012757736252	26.355003878237614
43	23.46000656683179	23.41396736570115	28.43271614631663	24.693309921150433
44	22.127367985267455	24.108719134700948	28.05940603482324	25.704506845208353
45	22.582168672405505	24.29228111750346	26.452435675979196	26.673114534111836
46	23.746948564576225	23.88078345158389	28.79603325291344	23.576234730926444
47	19.590929206698167	24.65119654337202	31.408133354271055	24.34974089565875
48	20.566079934522026	23.475828824584696	29.5478879070365	26.410203333856774
49	22.608578756775017	21.122238242753884	31.39944895715856	24.869734043312537
50	20.740838555868034	22.569796380628798	30.30795110089604	26.381413962607127
51	19.934141339157826	23.051007152136364	26.786249625262315	30.228601883443496
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	613.0
1	780.5
2	948.0
3	1328.5
4	1709.0
5	1350.0
6	991.0
7	1049.5
8	1108.0
9	1188.5
10	1269.0
11	1317.5
12	1366.0
13	1366.0
14	1366.0
15	1448.5
16	1531.0
17	1453.0
18	1375.0
19	1629.0
20	1883.0
21	2208.0
22	2533.0
23	2455.5
24	2378.0
25	3500.5
26	4648.0
27	4673.0
28	5587.0
29	6501.0
30	8826.5
31	11152.0
32	12421.0
33	13690.0
34	15630.5
35	17571.0
36	19193.5
37	20816.0
38	22998.0
39	25180.0
40	30946.5
41	36713.0
42	41664.5
43	46616.0
44	54080.0
45	61544.0
46	72943.5
47	84343.0
48	93746.5
49	103150.0
50	100472.5
51	97795.0
52	83470.0
53	69145.0
54	58792.0
55	48439.0
56	43197.0
57	37955.0
58	34970.0
59	31985.0
60	29715.5
61	27446.0
62	25142.5
63	22839.0
64	19369.0
65	15899.0
66	13418.0
67	10937.0
68	9634.0
69	8331.0
70	6818.0
71	5305.0
72	4367.0
73	3429.0
74	2947.5
75	2055.0
76	1644.0
77	1212.5
78	781.0
79	476.5
80	172.0
81	225.5
82	279.0
83	168.5
84	58.0
85	43.0
86	28.0
87	16.5
88	5.0
89	4.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	840588.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.894120397471614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.98791507098709	15.107352724133097
2	12.861297934078575	5.888962067410956
3	5.929350334124447	4.072417812847009
4	3.2827674740552495	3.0062429515169864
5	2.112098800257196	2.4177322112221797
6	1.4273695155902055	1.9607021724961742
7	0.9918190703621004	1.5894777646564808
8	0.7763795964672475	1.4219622364529274
9	0.5559137445301968	1.1454440578095244
>10	4.037916764565451	20.268786388715025
>50	1.5626898686816686	26.100779760412983
>100	0.4655491673337223	14.222265798119718
>500	0.006305406329576374	1.0482704664355997
>1k	0.0026272526373234896	1.7496035877713294
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	4304	0.5120225366053287	TruSeq Adapter, Index 22 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	4101	0.48787277477194535	TruSeq Adapter, Index 22 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGCT	3206	0.3813996868858466	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	1673	0.19902734752340032	TruSeq Adapter, Index 22 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTGC	1260	0.149895073448586	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCC	939	0.1117075190223986	No Hit
ACCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTG	930	0.11063683992633727	No Hit
GCCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTG	927	0.11027994689431683	TruSeq Adapter, Index 22 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTCTG	918	0.10920926779825552	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3975788376707733	0.0
2	0.0	0.0	0.0	1.818488962488163	0.0
3	0.0	0.0	0.0	2.5697487948911952	0.0
4	0.0	0.0	0.0	3.441757436461144	0.0
5	0.0	0.0	0.0	5.720162552879651	0.0
6	0.0	0.0	0.0	6.880302835634104	0.0
7	0.0	0.0	0.0	8.060310163837695	0.0
8	0.0	0.0	0.0	9.840849500587684	0.0
9	0.0	0.0	0.0	10.479688027904276	0.0
10	0.0	0.0	0.0	12.028603786873	0.0
11	0.0	0.0	0.0	14.429661141962532	0.0
12	0.0	0.0	0.0	16.234350240545904	0.0
13	0.0	0.0	0.0	16.94742251852275	0.0
14	0.0	0.0	0.0	17.22960594250691	0.0
15	1.1896434400681428E-4	0.0	0.0	17.781838427386543	0.0
16	1.1896434400681428E-4	0.0	0.0	18.876786249625262	0.0
17	1.1896434400681428E-4	0.0	0.0	20.144232370673862	0.0
18	4.758573760272571E-4	0.0	0.0	21.586912970444498	0.0
19	4.758573760272571E-4	0.0	0.0	22.417522020300076	0.0
20	5.948217200340714E-4	0.0	0.0	23.223743379634257	0.0
21	5.948217200340714E-4	0.0	0.0	24.177956382912914	0.0
22	5.948217200340714E-4	0.0	0.0	25.180944767234365	0.0
23	5.948217200340714E-4	0.0	0.0	26.12242858570429	0.0
24	5.948217200340714E-4	0.0	0.0	26.85786616035442	0.0
25	5.948217200340714E-4	0.0	0.0	27.49254093563077	0.0
26	5.948217200340714E-4	0.0	0.0	28.072611077008	0.0
27	5.948217200340714E-4	0.0	0.0	28.673262049898405	0.0
28	5.948217200340714E-4	0.0	0.0	29.24881154620337	0.0
29	5.948217200340714E-4	0.0	0.0	29.851128019909872	0.0
30	5.948217200340714E-4	0.0	0.0	30.5219679557643	0.0
31	7.137860640408857E-4	0.0	0.0	31.156523766696644	0.0
32	7.137860640408857E-4	0.0	0.0	31.74706277034647	0.0
33	8.327504080477E-4	0.0	0.0	32.31095376093877	0.0
34	8.327504080477E-4	0.0	0.0	32.93634931738259	0.0
35	9.517147520545142E-4	0.0	0.0	33.54627950910553	0.0
36	9.517147520545142E-4	0.0	0.0	34.12301864885057	0.0
37	9.517147520545142E-4	0.0	0.0	34.67858213536239	0.0
38	9.517147520545142E-4	0.0	0.0	35.279827929972825	0.0
39	9.517147520545142E-4	0.0	0.0	36.02954122590377	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.0322026E-4	45.000004	25
CTATGCG	20	7.0322026E-4	45.000004	1
GCCTTCG	20	7.0322026E-4	45.000004	30
TACCCGT	20	7.0322026E-4	45.000004	35
CGTATCG	20	7.0322026E-4	45.000004	13
CGACGGT	20	7.0322026E-4	45.000004	28
TTAGCCG	20	7.0322026E-4	45.000004	30
CGCATGT	20	7.0322026E-4	45.000004	38
TATATCG	20	7.0322026E-4	45.000004	29
GTCTACG	20	7.0322026E-4	45.000004	38
AACGGTC	40	6.8121153E-9	45.000004	15
TAGCACG	65	0.0	45.000004	1
ATATCGA	20	7.0322026E-4	45.000004	30
TGCGCAC	20	7.0322026E-4	45.000004	10
CTACGCA	20	7.0322026E-4	45.000004	40
TCGCTCA	20	7.0322026E-4	45.000004	19
AGCGCGA	20	7.0322026E-4	45.000004	40
TGTTACG	20	7.0322026E-4	45.000004	1
AACGAAG	40	6.8121153E-9	45.000004	1
TCGTATC	20	7.0322026E-4	45.000004	12
>>END_MODULE