SRR2935024.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935024.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1639952
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	8304	0.5063562835985443	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	7153	0.4361713025747095	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT	5511	0.33604642087085473	TruSeq Adapter, Index 22 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	3007	0.1833590251421993	TruSeq Adapter, Index 20 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	2286	0.13939432373630448	TruSeq Adapter, Index 22 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTG	1669	0.10177127135428353	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGTA	150	0.0	45.000004	5
TCGGCGA	25	3.8915372E-5	45.0	19
TACGCAC	25	3.8915372E-5	45.0	24
TGCGATG	20	7.0341694E-4	45.0	1
ACTACGC	40	6.8175723E-9	45.0	23
TCGTAGA	20	7.0341694E-4	45.0	13
ATACGAG	40	6.8175723E-9	45.0	20
CCCGGTA	25	3.8915372E-5	45.0	21
CGTCCAG	20	7.0341694E-4	45.0	31
TATCGTG	70	0.0	45.0	1
CCGCATA	25	3.8915372E-5	45.0	15
CCGTTAG	20	7.0341694E-4	45.0	36
GCGTAAG	90	0.0	45.0	1
TCGCGGA	25	3.8915372E-5	45.0	30
GCATCGA	20	7.0341694E-4	45.0	15
TACGTAG	45	3.8562575E-10	45.0	1
TACCGGC	25	3.8915372E-5	45.0	37
CCCGAAT	20	7.0341694E-4	45.0	12
CGGTCTA	65	0.0	41.53846	31
AGTACGG	225	0.0	41.0	2