SRR2935023.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935023.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620924
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	4563	0.73487254478809	RNA PCR Primer, Index 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	4363	0.702662483653394	RNA PCR Primer, Index 23 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGCT	2643	0.42565595789500804	Illumina Paired End PCR Primer 2 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	1640	0.2641225013045075	RNA PCR Primer, Index 23 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTGC	1201	0.1934214171138497	RNA PCR Primer, Index 23 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGC	910	0.14655577816286694	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTC	861	0.13866431318486644	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCG	857	0.1380201119621725	No Hit
CGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTG	818	0.13173915004090678	Illumina Paired End PCR Primer 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCC	735	0.11837197467000793	No Hit
GCCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTG	735	0.11837197467000793	RNA PCR Primer, Index 23 (95% over 22bp)
ACCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTG	674	0.10854790602392564	RNA PCR Primer, Index 23 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTCTG	647	0.10419954777074168	Illumina Paired End PCR Primer 2 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTCA	35	1.2107193E-7	45.000004	6
AGACTAC	45	3.8380676E-10	45.000004	22
ATAGGAT	45	3.8380676E-10	45.000004	12
TTTACGG	90	0.0	45.000004	2
CGAAAGG	45	3.8380676E-10	45.000004	2
GCGAAAG	35	1.2107193E-7	45.000004	1
CGTGAGG	35	1.2107193E-7	45.000004	2
TCTCGGG	45	3.8380676E-10	45.000004	3
ACAATCG	35	1.2107193E-7	45.000004	22
GAAACGG	45	3.8380676E-10	45.000004	2
ACTACAA	45	3.8380676E-10	45.000004	24
GTCCGAC	35	1.2107193E-7	45.000004	10
GCGTCGC	45	3.8380676E-10	45.000004	1
GGCCTAT	35	1.2107193E-7	45.000004	8
ACGCGAT	25	3.888727E-5	45.0	22
TCACGAG	20	7.0307776E-4	45.0	36
TCGCCTA	20	7.0307776E-4	45.0	16
AACGTAC	25	3.888727E-5	45.0	17
CGGGTCG	20	7.0307776E-4	45.0	6
CGTGGAG	25	3.888727E-5	45.0	25