SRR2935022.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935022.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	742264
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	6611	0.8906534602243946	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	6126	0.8253128267031676	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	4603	0.6201297651509436	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	2901	0.3908312945259369	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	2849	0.3838257008288156	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	2807	0.37816733668883307	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG	2661	0.35849778515460806	No Hit
ACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	2086	0.28103208561913284	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	1728	0.23280126747356736	No Hit
ACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	1282	0.17271482922518133	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC	1277	0.17204121444661197	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1115	0.15021609562096505	No Hit
GCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	1104	0.14873414310811248	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	1099	0.1480605283295431	No Hit
AAAACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	1057	0.1424021641895606	No Hit
GGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	907	0.12219372083248009	No Hit
AGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	893	0.1203075994524859	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	770	0.10373667589967991	No Hit
GGCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT	755	0.10171583156397186	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	752	0.10131166269683024	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAT	55	1.8189894E-12	45.000004	33
CTACTTA	30	2.1644573E-6	45.000004	26
CGTGCAC	30	2.1644573E-6	45.000004	35
CACATAG	30	2.1644573E-6	45.000004	10
TTCGTCC	35	1.2111559E-7	45.000004	26
TGTATAC	35	1.2111559E-7	45.000004	23
GTCTAGA	30	2.1644573E-6	45.000004	35
CACGAAA	30	2.1644573E-6	45.000004	40
CATTCGT	30	2.1644573E-6	45.000004	10
TATGCGA	35	1.2111559E-7	45.000004	1
ACGGGTA	55	1.8189894E-12	45.000004	5
TAGTTAG	30	2.1644573E-6	45.000004	1
ATATACG	30	2.1644573E-6	45.000004	10
AACGCTC	30	2.1644573E-6	45.000004	17
CTCGCGG	30	2.1644573E-6	45.000004	2
GGGCCTA	30	2.1644573E-6	45.000004	7
TCGTAAG	35	1.2111559E-7	45.000004	1
CTATTAG	30	2.1644573E-6	45.000004	1
GACATAC	30	2.1644573E-6	45.000004	12
TATAGAG	30	2.1644573E-6	45.000004	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content