##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935022.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	742264
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.060749275190496	33.0	31.0	34.0	30.0	34.0
2	32.24042793399653	34.0	31.0	34.0	30.0	34.0
3	32.25195617731696	34.0	31.0	34.0	30.0	34.0
4	35.82771897869222	37.0	35.0	37.0	35.0	37.0
5	35.73312864425595	37.0	35.0	37.0	35.0	37.0
6	35.65041952728409	37.0	35.0	37.0	33.0	37.0
7	35.99372864641152	37.0	35.0	37.0	35.0	37.0
8	35.761303255984394	37.0	35.0	37.0	35.0	37.0
9	37.5188814222433	39.0	37.0	39.0	35.0	39.0
10	37.33657431857129	39.0	37.0	39.0	34.0	39.0
11	37.221981666900184	39.0	37.0	39.0	34.0	39.0
12	36.820073450955455	39.0	35.0	39.0	33.0	39.0
13	36.489098218423635	39.0	35.0	39.0	33.0	39.0
14	37.13788625071403	39.0	35.0	41.0	32.0	41.0
15	37.29848544453186	39.0	35.0	41.0	32.0	41.0
16	37.5339218930192	39.0	35.0	41.0	33.0	41.0
17	37.62312600368602	39.0	35.0	41.0	33.0	41.0
18	37.66519728829635	39.0	35.0	41.0	33.0	41.0
19	37.63477280320748	39.0	35.0	41.0	33.0	41.0
20	37.520970975286424	39.0	35.0	41.0	33.0	41.0
21	37.478899690676094	39.0	35.0	41.0	33.0	41.0
22	37.45322823146481	39.0	35.0	41.0	33.0	41.0
23	37.23940942845133	39.0	35.0	41.0	33.0	41.0
24	36.997681417932164	39.0	35.0	41.0	32.0	41.0
25	36.97800917193882	39.0	35.0	40.0	32.0	41.0
26	37.121305896554325	39.0	35.0	40.0	33.0	41.0
27	37.16187906189712	39.0	35.0	41.0	33.0	41.0
28	37.17854294428936	39.0	35.0	41.0	33.0	41.0
29	37.17827484560749	39.0	35.0	41.0	33.0	41.0
30	37.029577077697425	39.0	35.0	40.0	32.0	41.0
31	36.8897386913551	39.0	35.0	40.0	32.0	41.0
32	36.795160481984844	38.0	35.0	40.0	32.0	41.0
33	36.68179380921074	38.0	35.0	40.0	32.0	41.0
34	36.55915550262441	38.0	35.0	40.0	31.0	41.0
35	36.475819923908475	38.0	35.0	40.0	31.0	41.0
36	36.35037803261373	38.0	35.0	40.0	31.0	41.0
37	36.27659565868748	38.0	35.0	40.0	31.0	41.0
38	36.230995979867004	38.0	35.0	40.0	31.0	41.0
39	36.18077934535421	38.0	35.0	40.0	31.0	41.0
40	36.10456118038865	38.0	35.0	40.0	31.0	41.0
41	36.007576819029346	38.0	35.0	40.0	30.0	41.0
42	36.00759433301359	38.0	35.0	40.0	30.0	41.0
43	35.92537426037097	38.0	35.0	40.0	30.0	41.0
44	35.81932573855124	38.0	35.0	40.0	30.0	41.0
45	35.83972683573499	38.0	35.0	40.0	30.0	41.0
46	35.747416013709405	37.0	35.0	40.0	30.0	41.0
47	35.62091789444187	37.0	35.0	40.0	30.0	41.0
48	35.598393563476066	37.0	35.0	40.0	30.0	41.0
49	35.571516333811154	37.0	35.0	40.0	30.0	41.0
50	35.47605703631053	37.0	34.0	40.0	29.0	41.0
51	34.36042432342131	36.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	10.0
10	5.0
11	7.0
12	11.0
13	12.0
14	8.0
15	34.0
16	65.0
17	138.0
18	267.0
19	413.0
20	720.0
21	1213.0
22	1875.0
23	2807.0
24	3967.0
25	4612.0
26	5389.0
27	6355.0
28	7698.0
29	9637.0
30	12198.0
31	16306.0
32	22485.0
33	34366.0
34	70278.0
35	80911.0
36	56412.0
37	88286.0
38	151953.0
39	163741.0
40	85.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.092608559757714	21.861089854822545	26.52196523069959	11.524336354720154
2	30.04672192104157	26.83640860933576	24.995554142461444	18.121315327161224
3	29.56225817229449	26.507819320349636	27.32895573542567	16.600966771930203
4	26.568175204509448	26.232715044781912	27.867982281236863	19.331127469471777
5	25.692745438280717	28.641830938857332	25.2953127189248	20.370110903937146
6	24.13278833406982	32.67476261815203	25.395007706153066	17.79744134162508
7	75.32751150534041	6.25262709763642	11.865589601543386	6.554271795479775
8	74.62641324380543	5.240588254313829	11.548047594925794	8.584950906954939
9	69.4595184462671	6.215308838903678	13.961474623584062	10.363698091245164
10	44.784739661360376	19.601651164545228	19.9821087914812	15.631500382613195
11	36.086486748650074	20.478023991463953	23.198349913238417	20.237139346647552
12	32.0061325889441	17.752039705549507	30.37167369022342	19.870154015282974
13	26.214662168716252	24.2967461711736	32.60174277615511	16.886848883955036
14	18.37567226754901	28.44742571376222	32.28945496481037	20.887447053878404
15	15.052865287822122	23.14944548031428	42.807680286259334	18.99000894560426
16	19.440657232467153	23.542028173264498	33.6496179256976	23.36769666857075
17	20.479775389888232	22.67037064979576	27.62440317730619	29.22545078300982
18	22.81587144196674	23.41013439962062	31.97662826164276	21.797365896769882
19	23.455131866829053	24.847089445264757	27.747539958828664	23.950238729077526
20	22.53497407930332	29.26923574361683	28.011731674983565	20.18405850209629
21	19.785278553183232	29.078468038325987	31.418066887253055	19.718186521237726
22	17.16033648405419	25.02317234838279	30.305255273056485	27.511235894506537
23	17.1478072491728	27.003869243288104	29.93395880710906	25.91436470043004
24	21.696862571807333	24.945841371803024	28.75863574146126	24.59866031492838
25	18.678933640860933	27.05519868941509	26.668004914693423	27.597862755030555
26	16.46166323572206	28.443518748046515	27.630465710313313	27.464352305918112
27	18.934098918983004	29.137341969972947	27.899238006962484	24.029321104081568
28	14.584703016716425	26.530183330998135	31.045692637659915	27.839421014625525
29	21.01880193569943	27.797387452442795	27.182511882564693	24.001298729293083
30	25.439466281538643	27.847234946056933	25.73801235140058	20.975286421003847
31	24.2044609465096	27.29581388832006	25.164631451882347	23.335093713287993
32	30.00388002112456	27.752255262278652	20.463069743379712	21.780794973217077
33	28.204250778698686	27.048866710496533	22.307831175969735	22.439051334835046
34	21.46096267635235	24.274786329392235	28.364571095998187	25.899679898257222
35	24.037808650291538	28.566116637746138	22.84348964788808	24.552585064074236
36	26.48370391127685	30.701340762855267	23.418891391742022	19.396063934125863
37	23.092457670047313	28.349212679046808	28.151304657103132	20.407024993802743
38	27.075272409816453	27.035798583792293	23.80285181552655	22.086077190864707
39	27.60756280784195	23.182722050375606	24.5599948266385	24.649720315143938
40	28.17286033001735	20.18540573165343	26.180981429787785	25.460752508541436
41	21.49895454986366	25.30770723085048	22.56151450157895	30.631823717706908
42	22.680609594430017	25.2350915577207	27.95245357446946	24.13184527337982
43	23.755159889203842	23.78223920330233	28.704477113264282	23.758123794229547
44	22.824493711132426	21.811377084164125	28.022509511440674	27.341619693262775
45	23.444084584460516	20.553064677796577	24.85934923423472	31.143501503508187
46	26.443152301606975	22.240200252201372	26.721624651067543	24.59502279512411
47	19.412095965855812	22.625238459631614	32.60268583684511	25.35997973766746
48	21.982744683832166	22.096450858454673	29.616416800491468	26.30438765722169
49	24.814082321114856	18.559973270965585	33.53052283284653	23.09542157507302
50	22.157749803304487	19.48363385533988	29.6021361671858	28.756480174169834
51	20.078166258905185	20.655049955271977	25.093497731265423	34.17328605455741
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1352.0
1	1340.0
2	1328.0
3	1599.0
4	1870.0
5	1442.0
6	1014.0
7	1074.5
8	1135.0
9	1295.5
10	1456.0
11	1525.0
12	1594.0
13	1705.0
14	1816.0
15	1958.5
16	2101.0
17	2005.5
18	1910.0
19	1973.5
20	2037.0
21	2076.0
22	2115.0
23	2132.0
24	2149.0
25	2054.0
26	2064.5
27	2170.0
28	3035.0
29	3900.0
30	3963.5
31	4027.0
32	5583.0
33	7139.0
34	7737.0
35	8335.0
36	9480.0
37	10625.0
38	11952.0
39	13279.0
40	17286.0
41	21293.0
42	28589.0
43	35885.0
44	44192.0
45	52499.0
46	70434.0
47	88369.0
48	100003.5
49	111638.0
50	109088.0
51	106538.0
52	87074.5
53	67611.0
54	57357.0
55	47103.0
56	40821.5
57	34540.0
58	30841.0
59	27142.0
60	23949.0
61	20756.0
62	18952.0
63	17148.0
64	14865.0
65	12582.0
66	11163.5
67	9745.0
68	7921.0
69	6097.0
70	4719.0
71	3341.0
72	2955.0
73	2569.0
74	2031.0
75	1399.5
76	1306.0
77	938.5
78	571.0
79	467.5
80	364.0
81	296.5
82	229.0
83	159.5
84	90.0
85	60.5
86	31.0
87	19.5
88	8.0
89	5.5
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	742264.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.551522172420384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.12297002899957	15.101935503994312
2	12.736202327822333	5.999139030322795
3	6.3010720873546076	4.451995169260537
4	3.7090318737278407	3.4941338564926068
5	2.379618956988695	2.802182431371556
6	1.690459203516062	2.3887732447908383
7	1.2378105557946488	2.040662592503757
8	0.9194781056030307	1.7324087192931894
9	0.6817255682253458	1.4450107352008605
>10	4.756304409360144	22.984342379524126
>50	1.0976966249389297	18.466950896549616
>100	0.34916217565091423	12.249320017054014
>500	0.009811168572009159	1.5005824787869098
>1k	0.00750265831977171	3.61132027888491
>5k	0.0011542551261187247	1.7312426659700064
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	6611	0.8906534602243946	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	6126	0.8253128267031676	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	4603	0.6201297651509436	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	2901	0.3908312945259369	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	2849	0.3838257008288156	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	2807	0.37816733668883307	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG	2661	0.35849778515460806	No Hit
ACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	2086	0.28103208561913284	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	1728	0.23280126747356736	No Hit
ACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	1282	0.17271482922518133	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC	1277	0.17204121444661197	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1115	0.15021609562096505	No Hit
GCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	1104	0.14873414310811248	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	1099	0.1480605283295431	No Hit
AAAACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	1057	0.1424021641895606	No Hit
GGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	907	0.12219372083248009	No Hit
AGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	893	0.1203075994524859	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	770	0.10373667589967991	No Hit
GGCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT	755	0.10171583156397186	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	752	0.10131166269683024	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6567744091051162	0.0
2	0.0	0.0	0.0	3.1828298287401786	0.0
3	0.0	0.0	0.0	4.377014108187923	0.0
4	0.0	0.0	0.0	5.7902579136264185	0.0
5	0.0	0.0	0.0	9.657480357393057	0.0
6	0.0	0.0	0.0	11.530398887727278	0.0
7	0.0	0.0	0.0	13.642989556276474	0.0
8	0.0	0.0	0.0	16.545595637131804	0.0
9	0.0	0.0	0.0	17.62324455988705	0.0
10	0.0	0.0	0.0	20.28604377957169	0.0
11	0.0	0.0	0.0	23.88557171033487	0.0
12	0.0	0.0	0.0	26.897438108274144	0.0
13	0.0	0.0	0.0	27.93359236066952	0.0
14	0.0	0.0	0.0	28.360933576193915	0.0
15	0.0	0.0	0.0	29.099754265328777	0.0
16	0.0	0.0	0.0	30.539942661910047	0.0
17	0.0	0.0	0.0	32.184371059353545	0.0
18	0.0	0.0	0.0	34.19591951111734	0.0
19	1.3472295571387E-4	0.0	0.0	35.35372859252234	0.0
20	1.3472295571387E-4	0.0	0.0	36.43232057596758	0.0
21	1.3472295571387E-4	0.0	0.0	37.60238944634254	0.0
22	1.3472295571387E-4	0.0	0.0	38.75709989976612	0.0
23	0.00242501320284966	0.0	0.0	39.86546565642413	0.0
24	0.00242501320284966	0.0	0.0	40.698322968647275	0.0
25	0.00242501320284966	0.0	0.0	41.42838666566074	0.0
26	0.00242501320284966	0.0	0.0	42.13446967655713	0.0
27	0.00242501320284966	0.0	0.0	42.9724464610974	0.0
28	0.00242501320284966	0.0	0.0	43.71988941939795	0.0
29	0.00242501320284966	0.0	0.0	44.454533696905685	0.0
30	0.00242501320284966	0.0	0.0	45.3298287401787	0.0
31	0.00255973615856353	0.0	0.0	45.99172262160094	0.0
32	0.00255973615856353	0.0	0.0	46.611178771973314	0.0
33	0.00255973615856353	0.0	0.0	47.242086373581365	0.0
34	0.00255973615856353	0.0	0.0	47.93806516279922	0.0
35	0.00255973615856353	0.0	0.0	48.648863477145596	0.0
36	0.00255973615856353	0.0	0.0	49.237063901792354	0.0
37	0.00255973615856353	0.0	0.0	49.87403403640753	0.0
38	0.00255973615856353	0.0	0.0	50.59601435607816	0.0
39	0.00255973615856353	0.0	0.0	51.786965284588774	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATAT	55	1.8189894E-12	45.000004	33
CTACTTA	30	2.1644573E-6	45.000004	26
CGTGCAC	30	2.1644573E-6	45.000004	35
CACATAG	30	2.1644573E-6	45.000004	10
TTCGTCC	35	1.2111559E-7	45.000004	26
TGTATAC	35	1.2111559E-7	45.000004	23
GTCTAGA	30	2.1644573E-6	45.000004	35
CACGAAA	30	2.1644573E-6	45.000004	40
CATTCGT	30	2.1644573E-6	45.000004	10
TATGCGA	35	1.2111559E-7	45.000004	1
ACGGGTA	55	1.8189894E-12	45.000004	5
TAGTTAG	30	2.1644573E-6	45.000004	1
ATATACG	30	2.1644573E-6	45.000004	10
AACGCTC	30	2.1644573E-6	45.000004	17
CTCGCGG	30	2.1644573E-6	45.000004	2
GGGCCTA	30	2.1644573E-6	45.000004	7
TCGTAAG	35	1.2111559E-7	45.000004	1
CTATTAG	30	2.1644573E-6	45.000004	1
GACATAC	30	2.1644573E-6	45.000004	12
TATAGAG	30	2.1644573E-6	45.000004	1
>>END_MODULE