SRR2935021.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935021.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1369652
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	10244	0.7479272107075374	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	7933	0.5791982196937616	TruSeq Adapter, Index 21 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGCT	5588	0.4079868462938031	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGC	4269	0.3116850119592422	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCG	3779	0.27590950109954937	No Hit
TCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	3646	0.2661990052947756	TruSeq Adapter, Index 21 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC	3528	0.25758367818978833	No Hit
ACTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	2697	0.19691133222161541	No Hit
GCCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTG	1682	0.12280491686939457	TruSeq Adapter, Index 21 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTG	1592	0.11623390467067546	No Hit
GGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTG	1584	0.11564981469745599	TruSeq Adapter, Index 21 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTG	1553	0.11338646605123053	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCC	1521	0.11105010615835263	No Hit
ACCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTG	1511	0.1103199936918283	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACAA	35	1.2121563E-7	45.000004	36
GTATTCG	35	1.2121563E-7	45.000004	32
GTCGAAC	45	3.8562575E-10	45.000004	6
ACGTTTA	45	3.8562575E-10	45.000004	11
ACGTTCA	35	1.2121563E-7	45.000004	21
TAGGTCG	45	3.8562575E-10	45.000004	1
ACGGGTA	45	3.8562575E-10	45.000004	5
CCCGTGA	35	1.2121563E-7	45.000004	33
TCATGCG	35	1.2121563E-7	45.000004	1
ATCCACG	35	1.2121563E-7	45.000004	1
CGCACCC	45	3.8562575E-10	45.000004	31
TGTCGAA	45	3.8562575E-10	45.000004	5
GCACTAG	35	1.2121563E-7	45.000004	1
AACGTGC	40	6.8175723E-9	45.0	15
GGTACGC	55	1.8189894E-12	45.0	8
TCGCACC	25	3.891199E-5	45.0	1
CGAACGG	40	6.8175723E-9	45.0	2
TCACCGT	25	3.891199E-5	45.0	28
CAACGAA	20	7.0337614E-4	45.0	25
CGCGGAT	20	7.0337614E-4	45.0	37