##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935021.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1369652
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20978394511891	33.0	31.0	34.0	30.0	34.0
2	32.38247525648851	34.0	31.0	34.0	30.0	34.0
3	32.386482113704794	34.0	31.0	34.0	30.0	34.0
4	35.94407119472684	37.0	35.0	37.0	35.0	37.0
5	35.86512413372156	37.0	35.0	37.0	35.0	37.0
6	35.80212929999737	37.0	35.0	37.0	35.0	37.0
7	36.081544071048704	37.0	35.0	37.0	35.0	37.0
8	35.760369787362045	37.0	35.0	37.0	35.0	37.0
9	37.50756104470332	39.0	37.0	39.0	35.0	39.0
10	37.39689278736497	39.0	37.0	39.0	34.0	39.0
11	37.3506978414955	39.0	37.0	39.0	35.0	39.0
12	37.17183488944637	39.0	37.0	39.0	34.0	39.0
13	37.013817378428975	39.0	37.0	39.0	33.0	39.0
14	38.084602512170974	40.0	37.0	41.0	33.0	41.0
15	38.17478819437346	40.0	37.0	41.0	33.0	41.0
16	38.27875401926913	40.0	37.0	41.0	34.0	41.0
17	38.280328871859425	40.0	37.0	41.0	34.0	41.0
18	38.27297882965892	40.0	37.0	41.0	34.0	41.0
19	38.24995911370188	40.0	37.0	41.0	34.0	41.0
20	38.19894980622815	40.0	37.0	41.0	34.0	41.0
21	38.16090437571003	40.0	36.0	41.0	34.0	41.0
22	38.07403340410557	40.0	36.0	41.0	34.0	41.0
23	37.86254829693966	40.0	36.0	41.0	33.0	41.0
24	37.65075800276274	39.0	35.0	41.0	33.0	41.0
25	37.59689176520751	39.0	35.0	41.0	33.0	41.0
26	37.78706196902571	39.0	36.0	41.0	34.0	41.0
27	37.83317076162412	40.0	36.0	41.0	34.0	41.0
28	37.80129186099827	40.0	36.0	41.0	34.0	41.0
29	37.86902585474267	40.0	36.0	41.0	34.0	41.0
30	37.781428421234004	40.0	36.0	41.0	34.0	41.0
31	37.624160005607266	40.0	36.0	41.0	33.0	41.0
32	37.538690849938526	40.0	35.0	41.0	33.0	41.0
33	37.491755570028005	40.0	35.0	41.0	33.0	41.0
34	37.34840382812568	40.0	35.0	41.0	33.0	41.0
35	37.28345375321614	39.0	35.0	41.0	33.0	41.0
36	37.24704231439811	39.0	35.0	41.0	33.0	41.0
37	37.146571537879694	39.0	35.0	41.0	32.0	41.0
38	37.04537137900722	39.0	35.0	41.0	32.0	41.0
39	37.051351730220524	39.0	35.0	41.0	32.0	41.0
40	36.94803570542006	39.0	35.0	41.0	32.0	41.0
41	36.8745126499286	39.0	35.0	41.0	32.0	41.0
42	36.87760686656173	39.0	35.0	41.0	32.0	41.0
43	36.81761717574975	39.0	35.0	41.0	32.0	41.0
44	36.689061893094014	39.0	35.0	41.0	31.0	41.0
45	36.68202944981645	39.0	35.0	41.0	31.0	41.0
46	36.530951657793366	39.0	35.0	41.0	31.0	41.0
47	36.443811274688755	39.0	35.0	40.0	31.0	41.0
48	36.41111318787546	39.0	35.0	40.0	31.0	41.0
49	36.38548770052539	39.0	35.0	40.0	31.0	41.0
50	36.311732469269565	38.0	35.0	40.0	31.0	41.0
51	35.23364621086232	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	11.0
10	19.0
11	14.0
12	18.0
13	21.0
14	33.0
15	40.0
16	82.0
17	161.0
18	302.0
19	580.0
20	1030.0
21	1592.0
22	2627.0
23	3741.0
24	5071.0
25	6510.0
26	7913.0
27	9569.0
28	11182.0
29	13839.0
30	17363.0
31	23219.0
32	31167.0
33	45794.0
34	90092.0
35	125568.0
36	99886.0
37	160399.0
38	294672.0
39	416804.0
40	328.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.19823721646082	22.116201779722147	24.699047641298666	10.986513362518362
2	31.43871581978488	25.709377272475052	25.264811791608377	17.58709511613169
3	30.997727890004178	25.428210961616525	26.39028015875565	17.183780989623642
4	28.632893610931827	26.7470861211461	26.460443966788645	18.159576301133427
5	25.719379813266436	30.167151948086087	23.821744501523014	20.291723737124467
6	26.61186929234579	33.30364209302801	23.796555621427924	16.28793299319827
7	76.78724230680494	6.9968137891960875	10.48317382809648	5.732770075902492
8	75.13404864885386	6.721926445549673	10.243988984063106	7.9000359215333535
9	68.68642545697739	8.509752842327831	12.971397114011443	9.832424586683333
10	39.01852441350066	26.133937671758957	19.57504533998417	15.272492574756216
11	32.00886064489374	23.749682401077063	23.867230508187482	20.374226445841717
12	28.889966210395052	21.0109575278976	30.386404721783343	19.71267153992401
13	24.4061265197291	26.247397149056837	29.93103357641211	19.41544275480195
14	17.50291314874143	30.74379477414701	28.775484575644033	22.977807501467527
15	16.329038325063593	26.352971411716258	36.93259309664061	20.385397166579537
16	18.437238072152635	26.16905608139878	32.57725320008294	22.816452646365644
17	17.707490661861552	26.033036128885296	28.540534383916498	27.718938825336654
18	19.313227009488543	26.645308443312608	31.490553804908107	22.550910742290743
19	21.48969227219761	27.604895258065554	27.349720951015293	23.555691518721545
20	23.0595070864716	28.550609935954537	28.622891070140444	19.76699190743342
21	22.089479663447356	28.08611238475175	28.96078711964791	20.863620832152986
22	18.865010966289248	26.993426067351418	27.02109732983269	27.120465636526653
23	19.131502016570632	27.383233113228762	28.739344008551075	24.74592086164953
24	22.35494855627561	23.926442629222606	29.025913151661882	24.692695662839903
25	18.78827614605754	28.53038582063181	27.12667159249211	25.554666440818547
26	18.435631824726283	28.293172280258048	29.319126318218057	23.952069576797612
27	20.730229284518987	28.318580194093094	29.405936690487806	21.545253830900112
28	15.889145563982677	28.32756057743135	31.019266207766645	24.76402765081933
29	18.87822600193334	25.37870933638618	30.25885407388154	25.484210587798945
30	22.452710615543218	25.714707093480683	29.767634406403964	22.06494788457214
31	22.58800045559018	29.74660716736806	24.1752649578141	23.490127419227658
32	21.873366373356152	29.19836571625493	25.151790381790413	23.776477528598505
33	24.025080823450043	26.598508234208396	24.396196990184368	24.98021395215719
34	21.296577524801922	26.359542423914977	27.63745827407254	24.70642177721056
35	19.94572343923858	25.42981720904288	27.87627806187265	26.748181289845892
36	22.30617704351178	27.4534699325084	27.090238980412547	23.15011404356727
37	20.786520955688015	28.109329961187225	29.04774351441096	22.056405568713807
38	20.106932271847157	29.288242560884083	23.87387453163285	26.730950635635914
39	25.030737734840674	23.09126697876541	26.400866789520254	25.477128496873657
40	25.413973768519305	22.79586347480966	29.33862032107426	22.45154243559678
41	19.8098495092184	26.232356832246438	27.29160399867996	26.666189659855206
42	20.477829404841522	24.955755184528623	29.12287208721632	25.443543323413536
43	22.57544252116596	22.79637455353623	29.007076250025555	25.62110667527226
44	21.25583724916986	23.77333804499245	27.763621708287943	27.20720299754974
45	22.511557680345078	22.383349931223407	26.790381790410994	28.314710598020522
46	24.47234772044286	24.234550090095876	27.584598131496175	23.708504057965087
47	19.3315528323983	24.178696486406768	32.01579671332572	24.47395396786921
48	19.863147719274675	22.637721114560485	29.841959855496142	27.657171310668694
49	20.962478060120382	20.614871514808144	32.35303566161332	26.069614763458166
50	20.51740150052714	21.212103512425053	30.56681551226151	27.7036794747863
51	19.516125263935656	21.287086062737103	27.18734393846028	32.00944473486696
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1530.0
1	1815.5
2	2101.0
3	2674.5
4	3248.0
5	2566.0
6	1884.0
7	1968.0
8	2052.0
9	2184.5
10	2317.0
11	2485.0
12	2653.0
13	2868.0
14	3083.0
15	3062.0
16	3041.0
17	3193.5
18	3346.0
19	3489.5
20	3633.0
21	3849.0
22	4065.0
23	4959.0
24	5853.0
25	6888.0
26	9468.5
27	11014.0
28	13412.0
29	15810.0
30	17558.5
31	19307.0
32	21583.5
33	23860.0
34	25570.0
35	27280.0
36	30400.5
37	33521.0
38	37675.5
39	41830.0
40	48230.0
41	54630.0
42	63109.5
43	71589.0
44	86735.0
45	101881.0
46	126265.0
47	150649.0
48	165449.0
49	180249.0
50	174982.0
51	169715.0
52	138068.5
53	106422.0
54	88895.0
55	71368.0
56	61786.0
57	52204.0
58	49310.5
59	46417.0
60	44754.0
61	43091.0
62	38590.0
63	34089.0
64	29047.0
65	24005.0
66	19765.5
67	15526.0
68	12621.0
69	9716.0
70	8499.5
71	7283.0
72	5946.5
73	4610.0
74	3888.5
75	2374.5
76	1582.0
77	1399.5
78	1217.0
79	741.0
80	265.0
81	299.0
82	333.0
83	232.5
84	132.0
85	110.5
86	89.0
87	75.5
88	62.0
89	33.5
90	5.0
91	4.0
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1369652.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.808748105475544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.00584708442636	12.480669540675125
2	12.491786159482823	4.94893290841322
3	6.216513495565589	3.6942404978384413
4	3.8497238162669105	3.05032837408325
5	2.5220192136100863	2.4979021659785863
6	1.8386400812747852	2.1852694935961985
7	1.326348899550282	1.8391317875816358
8	0.9953986404860713	1.5774080747137118
9	0.7883794044178818	1.405512813029279
>10	4.876787064854953	18.75054512713595
>50	1.2565571006481164	18.28815663886705
>100	0.8070688356412319	22.472565751526318
>500	0.01562788893355885	2.0876737993107004
>1k	0.008186037060435587	2.970018914815163
>5k	7.441851873123261E-4	0.9965907866290066
>10k+	3.7209259365616305E-4	0.7550533258063414
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	10244	0.7479272107075374	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	7933	0.5791982196937616	TruSeq Adapter, Index 21 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGCT	5588	0.4079868462938031	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGC	4269	0.3116850119592422	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCG	3779	0.27590950109954937	No Hit
TCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	3646	0.2661990052947756	TruSeq Adapter, Index 21 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC	3528	0.25758367818978833	No Hit
ACTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	2697	0.19691133222161541	No Hit
GCCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTG	1682	0.12280491686939457	TruSeq Adapter, Index 21 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTG	1592	0.11623390467067546	No Hit
GGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTG	1584	0.11564981469745599	TruSeq Adapter, Index 21 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTG	1553	0.11338646605123053	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCC	1521	0.11105010615835263	No Hit
ACCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTG	1511	0.1103199936918283	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1903373995730302E-4	0.0	0.0	0.432518625169021	0.0
2	2.1903373995730302E-4	0.0	0.0	2.3352647241781126	0.0
3	2.1903373995730302E-4	0.0	0.0	3.2495115547598954	0.0
4	2.1903373995730302E-4	0.0	0.0	4.356289042764147	0.0
5	2.1903373995730302E-4	0.0	0.0	7.485039995560916	0.0
6	2.1903373995730302E-4	0.0	0.0	8.938985961397494	0.0
7	2.1903373995730302E-4	0.0	0.0	10.575752088851766	0.0
8	2.1903373995730302E-4	0.0	0.0	12.864873705145541	0.0
9	2.1903373995730302E-4	0.0	0.0	13.650839775358996	0.0
10	2.1903373995730302E-4	0.0	0.0	15.76480741093358	0.0
11	2.1903373995730302E-4	0.0	0.0	18.48513344995663	0.0
12	2.1903373995730302E-4	0.0	0.0	20.898739241792807	0.0
13	2.1903373995730302E-4	0.0	0.0	21.70149789873632	0.0
14	2.1903373995730302E-4	0.0	0.0	22.036108442144428	0.0
15	2.1903373995730302E-4	0.0	0.0	22.720734902004306	0.0
16	2.1903373995730302E-4	0.0	0.0	24.02048111491094	0.0
17	2.1903373995730302E-4	0.0	0.0	25.540648281461277	0.0
18	2.1903373995730302E-4	0.0	0.0	27.193549894425736	0.0
19	2.1903373995730302E-4	0.0	0.0	28.194242041044003	0.0
20	2.1903373995730302E-4	0.0	0.0	29.1419280225926	0.0
21	2.1903373995730302E-4	0.0	0.0	30.200518087806245	0.0
22	2.1903373995730302E-4	0.0	0.0	31.331462298452454	0.0
23	2.1903373995730302E-4	0.0	0.0	32.39012537491275	0.0
24	2.1903373995730302E-4	0.0	0.0	33.242385657086615	0.0
25	2.1903373995730302E-4	0.0	0.0	33.99367138514017	0.0
26	3.650562332621717E-4	0.0	0.0	34.642595345387	0.0
27	3.650562332621717E-4	0.0	0.0	35.29889344154574	0.0
28	4.3806747991460604E-4	0.0	0.0	35.9307327700759	0.0
29	4.3806747991460604E-4	0.0	0.0	36.58929421488086	0.0
30	4.3806747991460604E-4	0.0	0.0	37.389716511931496	0.0
31	4.3806747991460604E-4	0.0	0.0	38.087923063668725	0.0
32	5.110787265670404E-4	0.0	0.0	38.7845233679796	0.0
33	5.110787265670404E-4	0.0	0.0	39.42819051846747	0.0
34	5.110787265670404E-4	0.0	0.0	40.04557362016045	0.0
35	5.110787265670404E-4	0.0	0.0	40.706982503584854	0.0
36	5.110787265670404E-4	0.0	0.0	41.38927260355185	0.0
37	5.110787265670404E-4	0.0	0.0	42.04133604740474	0.0
38	5.110787265670404E-4	0.0	0.0	42.63951719122814	0.0
39	5.110787265670404E-4	0.0	0.0	43.26938521609869	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACAA	35	1.2121563E-7	45.000004	36
GTATTCG	35	1.2121563E-7	45.000004	32
GTCGAAC	45	3.8562575E-10	45.000004	6
ACGTTTA	45	3.8562575E-10	45.000004	11
ACGTTCA	35	1.2121563E-7	45.000004	21
TAGGTCG	45	3.8562575E-10	45.000004	1
ACGGGTA	45	3.8562575E-10	45.000004	5
CCCGTGA	35	1.2121563E-7	45.000004	33
TCATGCG	35	1.2121563E-7	45.000004	1
ATCCACG	35	1.2121563E-7	45.000004	1
CGCACCC	45	3.8562575E-10	45.000004	31
TGTCGAA	45	3.8562575E-10	45.000004	5
GCACTAG	35	1.2121563E-7	45.000004	1
AACGTGC	40	6.8175723E-9	45.0	15
GGTACGC	55	1.8189894E-12	45.0	8
TCGCACC	25	3.891199E-5	45.0	1
CGAACGG	40	6.8175723E-9	45.0	2
TCACCGT	25	3.891199E-5	45.0	28
CAACGAA	20	7.0337614E-4	45.0	25
CGCGGAT	20	7.0337614E-4	45.0	37
>>END_MODULE