SRR2935019.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935019.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	424415
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	2797	0.6590247752789133	RNA PCR Primer, Index 17 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	2535	0.5972927441301557	RNA PCR Primer, Index 47 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGCT	1978	0.4660532733291707	RNA PCR Primer, Index 17 (96% over 25bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCG	1028	0.24221575580504928	No Hit
TCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	977	0.23019921539059648	RNA PCR Primer, Index 47 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGC	911	0.21464839838365748	No Hit
ACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTGC	909	0.21417716150465937	TruSeq Adapter, Index 9 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC	883	0.20805108207768339	RNA PCR Primer, Index 17 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTG	634	0.14938209064241367	RNA PCR Primer, Index 17 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCC	562	0.13241756299848026	No Hit
AGACATAGACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCC	535	0.12605586513200523	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	535	0.12605586513200523	No Hit
ACCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTG	505	0.11898731194703298	TruSeq Adapter, Index 9 (95% over 22bp)
AAAACTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTC	483	0.11380370627805331	RNA PCR Primer, Index 17 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGTGAGATGTCGTATGCCGTCTTCTG	471	0.11097628500406442	RNA PCR Primer, Index 47 (95% over 22bp)
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	460	0.1083844821695746	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAAAAG	40	6.7975634E-9	45.000004	15
TTTAGTC	20	7.028251E-4	45.000004	31
GCCCCGC	20	7.028251E-4	45.000004	10
GTTGACT	20	7.028251E-4	45.000004	12
CTGCTAC	20	7.028251E-4	45.000004	44
AGCGTTA	20	7.028251E-4	45.000004	17
TTCTAGC	20	7.028251E-4	45.000004	14
CTAGAGT	20	7.028251E-4	45.000004	13
AACGTAC	20	7.028251E-4	45.000004	8
GAAATCC	20	7.028251E-4	45.000004	28
CGGGTAG	65	0.0	45.000004	6
AGACTAA	20	7.028251E-4	45.000004	10
GCCCATC	20	7.028251E-4	45.000004	11
TCGTTAG	20	7.028251E-4	45.000004	1
GTACCGG	20	7.028251E-4	45.000004	2
CAAACGC	20	7.028251E-4	45.000004	13
AAATCCG	20	7.028251E-4	45.000004	29
CGTATGT	20	7.028251E-4	45.000004	32
CTCCGAA	20	7.028251E-4	45.000004	35
ATGGCTA	20	7.028251E-4	45.000004	45

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content