SRR2935018.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935018.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1155328
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	5722	0.49527060713494353	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	5236	0.4532046310658099	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT	3538	0.3062333813427875	Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	2141	0.18531533902060715	TruSeq Adapter, Index 14 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	1794	0.155280578329271	No Hit
GCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	1190	0.1030010525149568	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGTC	45	3.8562575E-10	45.000004	25
CATAGTA	45	3.8562575E-10	45.000004	3
CACAACG	90	0.0	45.000004	12
TGCGTTG	90	0.0	45.000004	1
ACAACGT	20	7.033303E-4	45.0	42
AACGTTG	25	3.890819E-5	45.0	1
AACGTTA	20	7.033303E-4	45.0	42
AACGTAC	35	1.211938E-7	45.0	12
TCGTTAG	80	0.0	45.0	1
GTCGAAT	20	7.033303E-4	45.0	31
CACGGTA	25	3.890819E-5	45.0	33
CGTGCTT	20	7.033303E-4	45.0	5
TCCGATT	20	7.033303E-4	45.0	19
GACGTAT	20	7.033303E-4	45.0	14
GACGTAA	20	7.033303E-4	45.0	37
CGCATAT	25	3.890819E-5	45.0	22
TGGCGAT	20	7.033303E-4	45.0	14
ATTCGTT	25	3.890819E-5	45.0	12
ATATGCG	35	1.211938E-7	45.0	1
CACGAAG	25	3.890819E-5	45.0	1