##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935018.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1155328
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27682441696211	33.0	31.0	34.0	30.0	34.0
2	32.45035349268779	34.0	31.0	34.0	31.0	34.0
3	32.46435730805451	34.0	31.0	34.0	30.0	34.0
4	35.97328983630623	37.0	35.0	37.0	35.0	37.0
5	35.89162471609794	37.0	35.0	37.0	35.0	37.0
6	35.82819337884999	37.0	35.0	37.0	35.0	37.0
7	36.08485728728119	37.0	35.0	37.0	35.0	37.0
8	35.82571442914912	37.0	35.0	37.0	35.0	37.0
9	37.55379684384002	39.0	38.0	39.0	35.0	39.0
10	37.38320286533348	39.0	37.0	39.0	34.0	39.0
11	37.282365700476404	39.0	37.0	39.0	34.0	39.0
12	37.06656724324175	39.0	37.0	39.0	34.0	39.0
13	36.94908285785508	39.0	35.0	39.0	33.0	39.0
14	38.05966271050299	40.0	37.0	41.0	33.0	41.0
15	38.14971246260802	40.0	37.0	41.0	33.0	41.0
16	38.20562558857744	40.0	37.0	41.0	34.0	41.0
17	38.22831351789275	40.0	37.0	41.0	34.0	41.0
18	38.21568074174607	40.0	37.0	41.0	34.0	41.0
19	38.18357903556392	40.0	36.0	41.0	34.0	41.0
20	38.156714803068915	40.0	36.0	41.0	34.0	41.0
21	38.0758511868491	40.0	36.0	41.0	34.0	41.0
22	38.02349635774429	40.0	36.0	41.0	34.0	41.0
23	37.81618380234877	40.0	35.0	41.0	33.0	41.0
24	37.591378379126965	39.0	35.0	41.0	33.0	41.0
25	37.54668025011079	39.0	35.0	41.0	33.0	41.0
26	37.723497569521385	39.0	35.0	41.0	33.0	41.0
27	37.77189075310215	40.0	36.0	41.0	34.0	41.0
28	37.77642106830268	40.0	36.0	41.0	34.0	41.0
29	37.81725276285176	40.0	36.0	41.0	34.0	41.0
30	37.745589131398184	40.0	36.0	41.0	34.0	41.0
31	37.659147878351426	40.0	35.0	41.0	33.0	41.0
32	37.5491323676047	40.0	35.0	41.0	33.0	41.0
33	37.47294447983603	40.0	35.0	41.0	33.0	41.0
34	37.36922847883891	40.0	35.0	41.0	33.0	41.0
35	37.28425520717926	40.0	35.0	41.0	33.0	41.0
36	37.22717704409484	39.0	35.0	41.0	33.0	41.0
37	37.17053165854199	39.0	35.0	41.0	33.0	41.0
38	37.07309872174828	39.0	35.0	41.0	32.0	41.0
39	37.0282387339353	39.0	35.0	41.0	32.0	41.0
40	36.97988276922225	39.0	35.0	41.0	32.0	41.0
41	36.91318482716596	39.0	35.0	41.0	32.0	41.0
42	36.87805714048305	39.0	35.0	41.0	32.0	41.0
43	36.803051600930644	39.0	35.0	41.0	32.0	41.0
44	36.685885739807226	39.0	35.0	41.0	31.0	41.0
45	36.67123881702858	39.0	35.0	41.0	31.0	41.0
46	36.54739866081321	39.0	35.0	41.0	31.0	41.0
47	36.4747491621427	39.0	35.0	41.0	31.0	41.0
48	36.41062278417904	38.0	35.0	40.0	31.0	41.0
49	36.33618331763793	38.0	35.0	40.0	31.0	41.0
50	36.26126693025704	38.0	35.0	40.0	31.0	41.0
51	35.22373386605362	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	20.0
10	27.0
11	16.0
12	16.0
13	22.0
14	31.0
15	60.0
16	84.0
17	121.0
18	316.0
19	524.0
20	843.0
21	1444.0
22	2191.0
23	3169.0
24	4677.0
25	5776.0
26	6987.0
27	8397.0
28	10046.0
29	12165.0
30	15055.0
31	19308.0
32	26318.0
33	37752.0
34	77518.0
35	110859.0
36	80080.0
37	125972.0
38	237340.0
39	367867.0
40	316.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.15835502991359	21.71392020274762	25.008828661644138	12.11889610569466
2	30.720972745402168	25.253953855528472	26.186070103035675	17.83900329603368
3	32.14455115776646	24.079741856857968	27.035352730999335	16.740354254376246
4	29.23819036671837	25.766968341458007	26.23047307777532	18.764368214048304
5	25.144634251052516	29.35296296809218	24.78932389762907	20.713078883226235
6	26.303439369598934	33.54458647241303	24.8198779913583	15.332096166629736
7	78.89084311987591	7.007533791269665	8.695625830932862	5.40599725792156
8	79.27973700974961	5.282742216928872	8.355722357633503	7.081798415688012
9	74.0259908874363	7.551708259472635	10.9512623255041	7.471038527586971
10	42.845841181032576	24.805163555284736	17.69021438067804	14.658780883004654
11	33.65615652005318	24.146043374695324	23.31519706957678	18.882603035674716
12	29.519236095723468	21.78454949590073	28.759711527808552	19.93650288056725
13	23.69154041103479	26.601536533348106	29.87731622534899	19.829606830268116
14	19.89348479392865	28.79641106248615	29.030543707068468	22.27956043651673
15	18.720311461333925	26.001014430534013	35.6065117438511	19.672162364280965
16	20.820234600044316	25.669506841347218	31.733325947263463	21.776932611345003
17	21.176323953024596	24.991431004874805	27.700964574562377	26.13128046753822
18	21.926500526257477	26.27028861068026	29.781585835364506	22.021625027697763
19	23.609226124529137	27.850099711943276	26.16815311322845	22.372521050299135
20	25.355310353423445	28.948921864613343	25.54980057611345	20.145967205849768
21	23.312946626412586	28.52722343784622	27.46025371150011	20.69957622424108
22	21.522026645247063	26.619193856636386	26.230386522269	25.628392975847554
23	21.53777974739641	27.21547473964104	27.492106137824063	23.75463937513849
24	23.058300326833592	24.825763765787727	28.840467815200533	23.27546809217815
25	20.894845446487924	27.49954991136716	26.534282904941282	25.071321737203633
26	19.371295424329716	28.614384832705518	27.367639319742963	24.646680423221802
27	21.066138793485486	27.289393142034125	26.743314452692225	24.901153611788168
28	17.80464076002659	27.052317610237093	28.459017698869932	26.684023930866385
29	19.46494848216264	24.588947900509638	28.830600487480613	27.115503129847106
30	21.595079492577	24.399824119211168	27.262560935076447	26.742535453135385
31	22.80625069244405	26.14244612785287	24.248438538666075	26.802864641037004
32	22.484264208951917	26.127125803235096	26.41440352869488	24.974206459118104
33	22.706019416131177	25.859755844227788	26.84718106027033	24.58704367937071
34	21.2601096831376	24.294659179038334	29.2767941225349	25.168437015289165
35	19.605601179924662	23.612601789275427	29.887616330600487	26.89418070019942
36	20.963743629514735	27.04885538998449	27.423900398847774	24.563500581653003
37	21.965796726124527	26.284743380234875	28.757201418125415	22.99225847551518
38	20.075943801240857	27.06737826833592	28.153130539552407	24.703547390870817
39	22.944739502548195	24.773917017504985	29.707840543984048	22.573502935962775
40	23.327314840460893	23.091364530245954	29.9198149789497	23.661505650343454
41	19.78797363173056	24.816675437624642	27.48111358852205	27.914237342122757
42	20.500152337691112	24.334647822955905	27.779643529802794	27.38555630955019
43	21.3444147462885	24.896479614447152	27.228371510081985	26.53073412918236
44	20.2384950421006	24.27570352315533	27.544731885663637	27.94106954908043
45	21.240461583204077	23.045922889430535	27.339855002215817	28.373760525149567
46	22.764011605362285	24.263585752271215	28.253967704409483	24.718434937957014
47	20.002717842898292	23.832798997341015	30.362719504764012	25.80176365499668
48	19.75508253933082	22.203478146465766	31.145181281852423	26.896258032350985
49	21.158320407711056	21.634116025925103	32.135635940616	25.071927625747843
50	19.595474185685795	22.080655883004656	31.039583564148014	27.28428636716153
51	19.039874390649235	22.319116302902724	28.354198980722355	30.286810325725682
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	842.0
1	1134.5
2	1427.0
3	1953.5
4	2480.0
5	1945.5
6	1411.0
7	1493.5
8	1576.0
9	1701.0
10	1826.0
11	2112.0
12	2398.0
13	2271.0
14	2144.0
15	2200.0
16	2256.0
17	2318.0
18	2380.0
19	2488.5
20	2597.0
21	2551.0
22	2505.0
23	2998.5
24	3492.0
25	4941.0
26	7645.0
27	8900.0
28	10755.0
29	12610.0
30	13634.0
31	14658.0
32	17245.0
33	19832.0
34	22097.5
35	24363.0
36	27052.0
37	29741.0
38	32059.0
39	34377.0
40	40754.5
41	47132.0
42	56593.0
43	66054.0
44	74614.0
45	83174.0
46	94948.0
47	106722.0
48	119882.5
49	133043.0
50	131276.5
51	129510.0
52	108827.5
53	88145.0
54	77856.0
55	67567.0
56	59504.5
57	51442.0
58	48822.5
59	46203.0
60	46491.0
61	46779.0
62	42622.5
63	38466.0
64	32431.5
65	26397.0
66	22080.0
67	17763.0
68	14236.0
69	10709.0
70	8358.5
71	6008.0
72	5555.0
73	5102.0
74	4294.0
75	2543.0
76	1600.0
77	1302.0
78	1004.0
79	699.0
80	394.0
81	346.5
82	299.0
83	194.0
84	89.0
85	54.0
86	19.0
87	12.0
88	5.0
89	4.5
90	4.0
91	5.0
92	6.0
93	3.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1155328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.445297328512705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.88595053542358	14.343890963323524
2	12.69521916563563	5.445054993153781
3	5.701028084909942	3.667807270772852
4	3.2996143625351935	2.830448442959926
5	1.957493329153612	2.09895132311397
6	1.2823388660837458	1.6500082955444262
7	0.9103196068188272	1.3665452242543172
8	0.6638257379884555	1.138875226038544
9	0.531264762733984	1.025381771729284
>10	3.8102975439573292	17.90506097797017
>50	1.465555623739747	22.993549761060407
>100	0.7856586788332456	22.400517989672526
>500	0.007350237120061543	1.0350906677844591
>1k	0.003266772053360686	1.139212732420307
>5k	8.166930133401715E-4	0.9596043602015315
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	5722	0.49527060713494353	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	5236	0.4532046310658099	TruSeq Adapter, Index 14 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGCT	3538	0.3062333813427875	Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	2141	0.18531533902060715	TruSeq Adapter, Index 14 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	1794	0.155280578329271	No Hit
GCCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTG	1190	0.1030010525149568	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4622202526035894E-4	0.0	0.0	0.3257083702636827	0.0
2	3.4622202526035894E-4	0.0	0.0	1.694583702636827	0.0
3	3.4622202526035894E-4	0.0	0.0	2.388152974739641	0.0
4	3.4622202526035894E-4	0.0	0.0	3.239859156880124	0.0
5	3.4622202526035894E-4	0.0	0.0	5.440532904941281	0.0
6	3.4622202526035894E-4	0.0	0.0	6.6117154608907605	0.0
7	3.4622202526035894E-4	0.0	0.0	7.833532988034567	0.0
8	3.4622202526035894E-4	0.0	0.0	9.632242964768446	0.0
9	3.4622202526035894E-4	0.0	0.0	10.220301074673166	0.0
10	3.4622202526035894E-4	0.0	0.0	11.578010746731664	0.0
11	3.4622202526035894E-4	0.0	0.0	13.624961915577222	0.0
12	3.4622202526035894E-4	0.0	0.0	15.198800686904498	0.0
13	3.4622202526035894E-4	0.0	0.0	15.822692776423665	0.0
14	3.4622202526035894E-4	0.0	0.0	16.065221305118545	0.0
15	5.193330378905384E-4	0.0	0.0	16.523619266563262	0.0
16	5.193330378905384E-4	0.0	0.0	17.45582206957678	0.0
17	5.193330378905384E-4	0.0	0.0	18.53127423554177	0.0
18	9.521105694659871E-4	0.0	0.0	19.867345031021493	0.0
19	9.521105694659871E-4	0.0	0.0	20.574157295590517	0.0
20	9.521105694659871E-4	0.0	0.0	21.250675132949258	0.0
21	9.521105694659871E-4	0.0	0.0	22.104891424772877	0.0
22	9.521105694659871E-4	0.0	0.0	22.928640178373588	0.0
23	9.521105694659871E-4	0.0	0.0	23.76554536893419	0.0
24	9.521105694659871E-4	0.0	0.0	24.47893585198316	0.0
25	9.521105694659871E-4	0.0	0.0	25.064224185685795	0.0
26	9.521105694659871E-4	0.0	0.0	25.609610430977177	0.0
27	9.521105694659871E-4	0.0	0.0	26.095446487923777	0.0
28	9.521105694659871E-4	0.0	0.0	26.617289635497453	0.0
29	9.521105694659871E-4	0.0	0.0	27.160165771105696	0.0
30	0.0010386660757810769	0.0	0.0	27.772026645247063	0.0
31	0.0010386660757810769	0.0	0.0	28.341388765787723	0.0
32	0.0011252215820961667	0.0	0.0	28.875522795258142	0.0
33	0.0011252215820961667	0.0	0.0	29.37780439840461	0.0
34	0.0011252215820961667	0.0	0.0	29.906918208508753	0.0
35	0.0011252215820961667	0.0	0.0	30.44970778861068	0.0
36	0.0011252215820961667	0.0	0.0	31.00271091845779	0.0
37	0.0011252215820961667	0.0	0.0	31.51953384666519	0.0
38	0.0011252215820961667	0.0	0.0	32.04700310214935	0.0
39	0.0011252215820961667	0.0	0.0	32.573433691557725	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCGTC	45	3.8562575E-10	45.000004	25
CATAGTA	45	3.8562575E-10	45.000004	3
CACAACG	90	0.0	45.000004	12
TGCGTTG	90	0.0	45.000004	1
ACAACGT	20	7.033303E-4	45.0	42
AACGTTG	25	3.890819E-5	45.0	1
AACGTTA	20	7.033303E-4	45.0	42
AACGTAC	35	1.211938E-7	45.0	12
TCGTTAG	80	0.0	45.0	1
GTCGAAT	20	7.033303E-4	45.0	31
CACGGTA	25	3.890819E-5	45.0	33
CGTGCTT	20	7.033303E-4	45.0	5
TCCGATT	20	7.033303E-4	45.0	19
GACGTAT	20	7.033303E-4	45.0	14
GACGTAA	20	7.033303E-4	45.0	37
CGCATAT	25	3.890819E-5	45.0	22
TGGCGAT	20	7.033303E-4	45.0	14
ATTCGTT	25	3.890819E-5	45.0	12
ATATGCG	35	1.211938E-7	45.0	1
CACGAAG	25	3.890819E-5	45.0	1
>>END_MODULE