SRR2935016.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935016.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2006381
Sequences flagged as poor quality	0
Sequence length	51
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	6108	0.304428720168303	Illumina PCR Primer Index 8 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	4582	0.22837138110857308	RNA PCR Primer, Index 40 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	3504	0.17464280213977307	RNA PCR Primer, Index 40 (96% over 26bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGC	20	7.034545E-4	45.000004	32
CGTCTAA	20	7.034545E-4	45.000004	45
CGTAGAT	20	7.034545E-4	45.000004	18
CGTACCA	20	7.034545E-4	45.000004	33
CCGTTCG	20	7.034545E-4	45.000004	27
GGCGTAT	25	3.8918493E-5	45.0	8
CGTACGA	25	3.8918493E-5	45.0	38
CGCAATA	25	3.8918493E-5	45.0	3
TCGTTAG	80	0.0	42.187504	1
CGGTCTA	80	0.0	42.187504	31
CCGGTAT	65	0.0	41.538464	42
CGGATGG	245	0.0	39.489796	2
TCGATAG	80	0.0	39.375004	1
ATACGTT	40	3.459936E-7	39.375004	12
TTCGATC	40	3.459936E-7	39.375004	38
TACGTAC	40	3.459936E-7	39.375004	12
ATTAGCG	115	0.0	39.130432	1
TGCTACG	105	0.0	38.57143	1
GTATGCG	130	0.0	38.076927	1
AATAGCG	65	9.094947E-12	38.076927	1