##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935016.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2006381
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.39802460250571	33.0	31.0	34.0	31.0	34.0
2	32.56376231632975	34.0	31.0	34.0	31.0	34.0
3	32.568806223743145	34.0	31.0	34.0	31.0	34.0
4	36.067259408856046	37.0	35.0	37.0	35.0	37.0
5	35.99837966966394	37.0	35.0	37.0	35.0	37.0
6	35.933457802879914	37.0	35.0	37.0	35.0	37.0
7	36.17709398165154	37.0	36.0	37.0	35.0	37.0
8	35.95006332296807	37.0	36.0	37.0	35.0	37.0
9	37.68996117885885	39.0	38.0	39.0	35.0	39.0
10	37.49900841365623	39.0	37.0	39.0	35.0	39.0
11	37.41101714978362	39.0	37.0	39.0	35.0	39.0
12	37.1740526849088	39.0	37.0	39.0	34.0	39.0
13	37.053585036939644	39.0	37.0	39.0	33.0	39.0
14	38.09803870750371	40.0	37.0	41.0	33.0	41.0
15	38.2174447425489	40.0	37.0	41.0	33.0	41.0
16	38.30050523803804	40.0	37.0	41.0	34.0	41.0
17	38.3294508869452	40.0	37.0	41.0	34.0	41.0
18	38.34111417522395	40.0	37.0	41.0	34.0	41.0
19	38.3156469284747	40.0	37.0	41.0	34.0	41.0
20	38.26476775846661	40.0	37.0	41.0	34.0	41.0
21	38.19492608831523	40.0	36.0	41.0	34.0	41.0
22	38.16111695635076	40.0	36.0	41.0	34.0	41.0
23	37.97415745065369	40.0	36.0	41.0	34.0	41.0
24	37.76711551794001	40.0	35.0	41.0	33.0	41.0
25	37.72065973511511	40.0	35.0	41.0	33.0	41.0
26	37.85772143974649	40.0	36.0	41.0	34.0	41.0
27	37.88737732265208	40.0	36.0	41.0	34.0	41.0
28	37.84718356084911	40.0	36.0	41.0	34.0	41.0
29	37.85606472549331	40.0	36.0	41.0	34.0	41.0
30	37.749750919690726	40.0	36.0	41.0	33.0	41.0
31	37.65929701288041	40.0	35.0	41.0	33.0	41.0
32	37.56842045453979	40.0	35.0	41.0	33.0	41.0
33	37.49657218643917	40.0	35.0	41.0	33.0	41.0
34	37.36380129197794	40.0	35.0	41.0	33.0	41.0
35	37.27665981685433	40.0	35.0	41.0	33.0	41.0
36	37.208242103568566	39.0	35.0	41.0	33.0	41.0
37	37.157338511479125	39.0	35.0	41.0	32.0	41.0
38	37.07503659574129	39.0	35.0	41.0	32.0	41.0
39	36.98419143721955	39.0	35.0	41.0	32.0	41.0
40	36.893114518129906	39.0	35.0	41.0	32.0	41.0
41	36.82343832003991	39.0	35.0	41.0	32.0	41.0
42	36.816571229492304	39.0	35.0	41.0	32.0	41.0
43	36.73671451234835	39.0	35.0	41.0	32.0	41.0
44	36.59845014481297	39.0	35.0	41.0	31.0	41.0
45	36.536911483910586	39.0	35.0	41.0	31.0	41.0
46	36.41989033987064	38.0	35.0	41.0	31.0	41.0
47	36.364358015750746	38.0	35.0	40.0	31.0	41.0
48	36.304629579327155	38.0	35.0	40.0	31.0	41.0
49	36.23194298590347	38.0	35.0	40.0	31.0	41.0
50	36.137853179431026	38.0	35.0	40.0	31.0	41.0
51	35.05725383165012	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	1.0
8	8.0
9	34.0
10	36.0
11	39.0
12	45.0
13	40.0
14	46.0
15	98.0
16	164.0
17	270.0
18	515.0
19	942.0
20	1521.0
21	2426.0
22	3671.0
23	5277.0
24	7246.0
25	9183.0
26	11354.0
27	13940.0
28	16652.0
29	20427.0
30	25794.0
31	33445.0
32	45384.0
33	68343.0
34	137486.0
35	176408.0
36	145030.0
37	228928.0
38	409065.0
39	642032.0
40	530.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.17533957907297	21.379090013312528	25.602664698280137	10.842905709334369
2	30.952595743281066	24.610231057810054	25.695867335266833	18.74130586364205
3	32.225584273375794	24.57748553240885	25.638151477710363	17.558778716504992
4	27.886228986418832	26.491080208594482	25.98050918544384	19.642181619542846
5	26.14647965665544	29.94326600979575	23.858778566982046	20.051475766566767
6	27.00459184970352	33.889027059167724	23.37347692188074	15.732904169248012
7	82.27151273860748	5.853723694552531	7.334050711205897	4.5407128556341
8	83.30885310417115	4.273714713207511	7.064261473767944	5.353170708853403
9	77.64482418842682	6.674654514770624	9.197455518169281	6.483065778633271
10	44.025387002767665	24.168839318155424	16.183616172601315	15.62215750647559
11	33.543130641687696	23.74803190420962	22.76287504716203	19.945962406940655
12	29.35080625265092	22.23142065240849	27.959295866537808	20.45847722840278
13	25.70817805790625	24.546384759425056	29.89820976175512	19.847227420913573
14	21.424345625282537	26.536236138599794	30.699453393946612	21.339964842171053
15	20.67030140337254	23.315362336465505	34.33914097073288	21.675195289429077
16	24.849766818964095	23.790745626079993	29.558543467068322	21.800944087887594
17	24.607739008692768	23.349154522495976	27.479277365565164	24.563829103246093
18	25.469190547558018	23.473707137378195	28.68607707110464	22.371025243959146
19	25.57874102675414	25.473277508110375	25.79355566066465	23.154425804470836
20	26.22981377913766	27.135025700502545	26.106806234708163	20.52835428565163
21	24.711159047060356	26.731812153324817	28.232673654704666	20.324355144910164
22	24.17452119014285	23.397201229477353	27.77174424997047	24.65653333040933
23	22.463629789157693	25.142183862387053	28.168827356319664	24.225358992135593
24	23.261534075532015	24.56961065719821	29.089240777300024	23.079614489969753
25	22.947286681841582	26.859255545183093	25.788422039483027	24.40503573349229
26	21.94623055142568	26.681921329996644	26.732360404130624	24.639487714447057
27	22.222648639515626	25.86228637531954	27.534202128110262	24.380862857054566
28	20.222729381907026	26.714068763609706	27.623018758650524	25.440183095832747
29	22.204307158012362	26.824964949329168	26.460926414275253	24.50980147838322
30	23.86072236529353	25.955738217217966	26.291965484122905	23.8915739333656
31	24.198095974792423	26.878793210262657	24.911270591178845	24.01184022376607
32	25.69756192866659	26.60940270068347	24.742808070849954	22.950227299799987
33	24.489416516603775	25.97074035290406	24.25830388146618	25.28153924902598
34	21.725484840616012	26.57177275901237	27.355223160506405	24.34751923986521
35	21.739789202549268	26.96915491125564	26.987097664900134	24.30395822129496
36	23.25106746923939	28.316007777186886	24.39551610586424	24.037408647709483
37	22.54197981340533	28.365798918550368	25.177770323781974	23.91445094426233
38	23.199332529564426	27.872672239220762	24.693714703239316	24.234280527975493
39	23.5231992328476	25.435149156615815	24.956875090025274	26.084776520511312
40	24.73099575803399	23.87447847642098	26.774824921089262	24.619700844455764
41	20.491621481662754	24.407876669486008	26.726479168213814	28.374022680637427
42	21.826362988883965	24.424473716607164	27.234109573406045	26.51505372110282
43	22.361555457313443	24.17945544739509	27.130390489144386	26.328598606147086
44	23.02792939127713	24.7028356030086	26.126892150593534	26.142342855120738
45	23.133442750903242	23.781275839434286	25.84813153633333	27.23714987332914
46	23.045024848221747	24.605944733328318	26.900723242494823	25.448307175955115
47	20.251986038544025	25.27705356061486	29.107980986662053	25.36297941417906
48	21.090411043565503	24.35793600517549	28.361412912103933	26.19024003915508
49	22.866594131423692	23.445945710211568	28.4781903337402	25.209269824624535
50	21.301387921835385	23.813473114029687	27.89943684674047	26.985702117394457
51	20.739480686868546	24.564626558963624	25.632818492599363	29.063074261568467
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	725.0
1	1330.0
2	1935.0
3	3090.5
4	4246.0
5	3202.0
6	2158.0
7	2362.5
8	2567.0
9	2625.0
10	2683.0
11	2864.0
12	3045.0
13	3257.5
14	3470.0
15	3614.5
16	3759.0
17	3710.5
18	3662.0
19	3757.5
20	3853.0
21	4068.5
22	4284.0
23	5291.5
24	6299.0
25	7600.0
26	10926.5
27	12952.0
28	15576.5
29	18201.0
30	21483.0
31	24765.0
32	28890.5
33	33016.0
34	37247.5
35	41479.0
36	46509.5
37	51540.0
38	57423.5
39	63307.0
40	70647.5
41	77988.0
42	89911.5
43	101835.0
44	112220.0
45	122605.0
46	141205.0
47	159805.0
48	177960.0
49	196115.0
50	196293.0
51	196471.0
52	176506.0
53	156541.0
54	139999.5
55	123458.0
56	118194.5
57	112931.0
58	107281.5
59	101632.0
60	96756.0
61	91880.0
62	83043.5
63	74207.0
64	66724.5
65	59242.0
66	51846.0
67	44450.0
68	37497.0
69	30544.0
70	26346.5
71	22149.0
72	18528.5
73	14908.0
74	12134.0
75	7844.5
76	6329.0
77	4946.5
78	3564.0
79	2637.0
80	1710.0
81	1308.0
82	906.0
83	673.5
84	441.0
85	401.0
86	361.0
87	208.5
88	56.0
89	39.5
90	23.0
91	19.5
92	16.0
93	11.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2006381.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.00440497100388
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.86256062519399	13.57550145115683
2	12.364114070328535	4.946734899410783
3	5.440476381049027	3.2650047828505913
4	2.93263224609971	2.346622523280132
5	1.8021264579252034	1.8025233736648276
6	1.1959537066567578	1.4354605364720976
7	0.8517559246761681	1.192720931757176
8	0.6657202570615507	1.065387009572805
9	0.4788242796576806	0.8620735320199312
>10	3.7623709470328057	16.475738459154773
>50	1.622600139158388	24.037184122040685
>100	1.0104789944346957	26.650950579762466
>500	0.0063329089789902395	0.7773457614783555
>1k	0.003799745387394144	1.2572326168368972
>5k	2.5331635915960956E-4	0.30951942054170767
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	6108	0.304428720168303	Illumina PCR Primer Index 8 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGC	4582	0.22837138110857308	RNA PCR Primer, Index 40 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCACAGACTCTCGTATGCCGTCTTCTGCT	3504	0.17464280213977307	RNA PCR Primer, Index 40 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.968196469165129E-5	0.0	0.0	0.18530877236177976	0.0
2	9.968196469165129E-5	0.0	0.0	0.939053948377701	0.0
3	9.968196469165129E-5	0.0	0.0	1.3600108852705444	0.0
4	9.968196469165129E-5	0.0	0.0	1.8629063971399251	0.0
5	9.968196469165129E-5	0.0	0.0	3.2663287780336834	0.0
6	9.968196469165129E-5	0.0	0.0	4.073952055965442	0.0
7	9.968196469165129E-5	0.0	0.0	4.890596551701796	0.0
8	9.968196469165129E-5	0.0	0.0	6.180780220705838	0.0
9	9.968196469165129E-5	0.0	0.0	6.649734023597712	0.0
10	9.968196469165129E-5	0.0	0.0	7.740404240271414	0.0
11	9.968196469165129E-5	0.0	0.0	9.305510767895031	0.0
12	9.968196469165129E-5	0.0	0.0	10.511114289858208	0.0
13	9.968196469165129E-5	0.0	0.0	10.981962050079222	0.0
14	9.968196469165129E-5	0.0	0.0	11.172055556746201	0.0
15	9.968196469165129E-5	0.0	0.0	11.531409039459604	0.0
16	9.968196469165129E-5	0.0	0.0	12.260084201355575	0.0
17	9.968196469165129E-5	0.0	0.0	13.128264272837512	9.968196469165129E-5
18	9.968196469165129E-5	0.0	0.0	14.082519720830689	9.968196469165129E-5
19	9.968196469165129E-5	0.0	0.0	14.664064302841783	9.968196469165129E-5
20	9.968196469165129E-5	0.0	0.0	15.23922923911261	9.968196469165129E-5
21	1.4952294703747692E-4	0.0	0.0	15.929975413443408	9.968196469165129E-5
22	1.4952294703747692E-4	0.0	0.0	16.64624017073527	9.968196469165129E-5
23	1.4952294703747692E-4	0.0	0.0	17.34590788090597	9.968196469165129E-5
24	1.4952294703747692E-4	0.0	0.0	17.92615659737607	9.968196469165129E-5
25	1.4952294703747692E-4	0.0	0.0	18.436129528738558	9.968196469165129E-5
26	1.4952294703747692E-4	0.0	0.0	18.91789246409331	9.968196469165129E-5
27	1.9936392938330258E-4	0.0	0.0	19.45901600942194	9.968196469165129E-5
28	1.9936392938330258E-4	0.0	0.0	19.926474582843436	9.968196469165129E-5
29	1.9936392938330258E-4	0.0	0.0	20.46490671512539	9.968196469165129E-5
30	2.492049117291282E-4	0.0	0.0	21.042264654619437	9.968196469165129E-5
31	2.492049117291282E-4	0.0	0.0	21.545210007471162	9.968196469165129E-5
32	2.492049117291282E-4	0.0	0.0	22.044018558788185	9.968196469165129E-5
33	2.492049117291282E-4	0.0	0.0	22.528423066207267	9.968196469165129E-5
34	2.492049117291282E-4	0.0	0.0	23.050058787438676	9.968196469165129E-5
35	2.492049117291282E-4	0.0	0.0	23.59641563591362	9.968196469165129E-5
36	2.492049117291282E-4	0.0	0.0	24.07758047948022	9.968196469165129E-5
37	2.492049117291282E-4	0.0	0.0	24.588550230489624	9.968196469165129E-5
38	2.9904589407495384E-4	0.0	0.0	25.177670641817283	9.968196469165129E-5
39	2.9904589407495384E-4	0.0	0.0	25.961818817064156	9.968196469165129E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGC	20	7.034545E-4	45.000004	32
CGTCTAA	20	7.034545E-4	45.000004	45
CGTAGAT	20	7.034545E-4	45.000004	18
CGTACCA	20	7.034545E-4	45.000004	33
CCGTTCG	20	7.034545E-4	45.000004	27
GGCGTAT	25	3.8918493E-5	45.0	8
CGTACGA	25	3.8918493E-5	45.0	38
CGCAATA	25	3.8918493E-5	45.0	3
TCGTTAG	80	0.0	42.187504	1
CGGTCTA	80	0.0	42.187504	31
CCGGTAT	65	0.0	41.538464	42
CGGATGG	245	0.0	39.489796	2
TCGATAG	80	0.0	39.375004	1
ATACGTT	40	3.459936E-7	39.375004	12
TTCGATC	40	3.459936E-7	39.375004	38
TACGTAC	40	3.459936E-7	39.375004	12
ATTAGCG	115	0.0	39.130432	1
TGCTACG	105	0.0	38.57143	1
GTATGCG	130	0.0	38.076927	1
AATAGCG	65	9.094947E-12	38.076927	1
>>END_MODULE