FastQCFastQC Report
Sat 14 Jan 2017
SRR2935015.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935015.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3678237
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC87670.2383478824230195Illumina PCR Primer Index 11 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC83540.2271196771714275Illumina PCR Primer Index 11 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT74010.20121052558603483Illumina PCR Primer Index 11 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64680.17584511275374587No Hit
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG41000.1114664443862644Illumina PCR Primer Index 11 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTA207.035312E-445.0000041
TCGATAG1550.039.1935461
CGTTTTT40100.038.771821
CCCGATA356.2520394E-638.57142620
GTTTGCG2500.037.8000031
TATTGCG2350.037.3404271
TACGGGA13200.037.1590924
CGGTCTA1900.036.71052631
GCGCGTA250.002107709936.03
CGACGGT1950.035.7692328
AACGAGC7800.034.61538315
AAATGCG1700.034.4117621
GCGTTAG2750.034.3636361
TCACGAC2100.034.28571325
TATAGCG1050.034.2857131
TTACACG14500.034.1379334
AGGGATC26250.033.7714276
GCTATCG803.6379788E-1233.7500041
CCGTCGA401.561497E-533.75000422
ACACGCG14650.033.6348136
Produced by FastQC (version 0.11.5)