##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935015.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3678237
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.350253667721795	33.0	31.0	34.0	30.0	34.0
2	32.52643073298431	34.0	31.0	34.0	31.0	34.0
3	32.524501819757674	34.0	31.0	34.0	31.0	34.0
4	36.02693491474312	37.0	35.0	37.0	35.0	37.0
5	35.955066516921015	37.0	35.0	37.0	35.0	37.0
6	35.901818180829565	37.0	35.0	37.0	35.0	37.0
7	36.16736523502971	37.0	35.0	37.0	35.0	37.0
8	35.953103891891686	37.0	36.0	37.0	35.0	37.0
9	37.67868084628587	39.0	38.0	39.0	35.0	39.0
10	37.49721945595132	39.0	37.0	39.0	35.0	39.0
11	37.40716598740103	39.0	37.0	39.0	35.0	39.0
12	37.21217284258736	39.0	37.0	39.0	34.0	39.0
13	37.12095223880353	39.0	37.0	39.0	33.0	39.0
14	38.07552612841424	40.0	37.0	41.0	33.0	41.0
15	38.212115750018285	40.0	37.0	41.0	33.0	41.0
16	38.33316722114426	40.0	37.0	41.0	34.0	41.0
17	38.38178725296929	40.0	37.0	41.0	34.0	41.0
18	38.40112341863779	40.0	37.0	41.0	34.0	41.0
19	38.36344313865583	40.0	37.0	41.0	34.0	41.0
20	38.304428996826466	40.0	37.0	41.0	34.0	41.0
21	38.25122660665966	40.0	37.0	41.0	34.0	41.0
22	38.249900427840835	40.0	37.0	41.0	34.0	41.0
23	38.05875885648478	40.0	36.0	41.0	34.0	41.0
24	37.848867541705445	40.0	36.0	41.0	33.0	41.0
25	37.81222254030939	40.0	36.0	41.0	33.0	41.0
26	37.96918088747408	40.0	36.0	41.0	34.0	41.0
27	38.01196796182519	40.0	36.0	41.0	34.0	41.0
28	37.97877869207449	40.0	36.0	41.0	34.0	41.0
29	37.98182770713252	40.0	36.0	41.0	34.0	41.0
30	37.88196655082312	40.0	36.0	41.0	34.0	41.0
31	37.77445662147382	40.0	36.0	41.0	33.0	41.0
32	37.71180377990869	40.0	36.0	41.0	33.0	41.0
33	37.641120188829596	40.0	36.0	41.0	33.0	41.0
34	37.50916920252828	40.0	35.0	41.0	33.0	41.0
35	37.40220600249522	40.0	35.0	41.0	33.0	41.0
36	37.356574358857245	40.0	35.0	41.0	33.0	41.0
37	37.284676055403715	40.0	35.0	41.0	32.0	41.0
38	37.218967130176765	40.0	35.0	41.0	32.0	41.0
39	37.15238196994919	39.0	35.0	41.0	32.0	41.0
40	37.064348490866685	39.0	35.0	41.0	32.0	41.0
41	36.975134826820565	39.0	35.0	41.0	32.0	41.0
42	36.975322144821014	39.0	35.0	41.0	32.0	41.0
43	36.89953964358468	39.0	35.0	41.0	32.0	41.0
44	36.78767436682302	39.0	35.0	41.0	31.0	41.0
45	36.7489454866557	39.0	35.0	41.0	32.0	41.0
46	36.65710039891393	39.0	35.0	41.0	31.0	41.0
47	36.58402055114991	39.0	35.0	41.0	31.0	41.0
48	36.50927767840952	39.0	35.0	41.0	31.0	41.0
49	36.4460484737661	39.0	35.0	41.0	31.0	41.0
50	36.35764362111522	38.0	35.0	41.0	31.0	41.0
51	35.25982202886872	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	17.0
9	64.0
10	60.0
11	59.0
12	61.0
13	62.0
14	97.0
15	154.0
16	286.0
17	484.0
18	903.0
19	1553.0
20	2584.0
21	4151.0
22	6196.0
23	9016.0
24	12952.0
25	17292.0
26	21828.0
27	26462.0
28	31648.0
29	38224.0
30	47717.0
31	62653.0
32	83689.0
33	127036.0
34	246143.0
35	263828.0
36	249416.0
37	402267.0
38	745445.0
39	1274804.0
40	1084.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.71586469278625	22.05980745667014	25.889386681717358	11.33494116882626
2	31.626401452652452	24.40960166514556	25.761988691865152	18.202008190336837
3	31.645459495948742	24.031540110112534	26.87991556824642	17.44308482569231
4	27.978566905830153	25.90749318219571	26.72883775569655	19.385102156277586
5	25.182335994118922	29.545295749023243	25.56360560779526	19.70876264906258
6	27.100428819567636	32.82341513067266	24.543198276783144	15.532957772976562
7	81.57391706950912	5.520987364326986	7.783429942116291	5.1216656240476075
8	82.76282904010807	4.117298586252055	7.684578236802034	5.435294136837839
9	76.96162590936908	6.854941647316363	10.033230593895933	6.150201849418621
10	41.718328644945935	27.422458096093315	16.74321148963484	14.116001769325903
11	30.63897187701608	23.77954438498661	25.879626571099145	19.70185716689816
12	27.904346566031496	21.65901762175738	29.93926166258455	20.497374149626573
13	26.226966886581803	22.52187664905769	31.867767085155197	19.38338937920531
14	20.438759112041993	25.352444663027423	32.77298336132228	21.4358128636083
15	19.611460599194668	24.139037261601143	34.97721870559183	21.27228343361235
16	24.819227254796253	24.98555693937068	29.793784359191644	20.40143144664142
17	25.111432460714195	24.583897122452957	27.723689365312786	22.580981051520062
18	25.75878063322184	23.294801286594637	29.96563299211008	20.980785088073446
19	24.900135581258088	26.105849079327946	27.204989781789486	21.78902555762448
20	23.422172089509186	28.774600440374016	27.872157231847755	19.93107023826904
21	23.276694786116284	27.048991133524027	30.37601981601512	19.298294264344577
22	22.173584790757094	23.92629947444931	29.847859178187814	24.052256556605787
23	20.2723478666546	26.568842627595775	30.099474286186563	23.059335219563067
24	21.45816052636086	26.237243549015467	29.412433184702348	22.892162739921325
25	23.302603937701676	26.285745045792318	26.9605520253317	23.451098991174305
26	21.706649136529265	25.98938023841313	26.908652161347952	25.395318463709653
27	21.590207482552103	24.7120019726842	30.34858819592103	23.349202348842667
28	19.631388624495923	25.382594976887024	29.35547655031473	25.630539848302327
29	22.700250147013364	25.890229476784665	27.5919142784981	23.817606097703873
30	24.121800743127753	24.67239060452059	29.10595483651543	22.09985381583623
31	23.658453764670412	26.028393493948325	28.269385577927686	22.04376716345358
32	25.85564769208727	25.57975464876244	26.355180484563668	22.20941717458663
33	24.4072092146319	24.837170633648675	26.9293686078412	23.82625154387822
34	22.19992893334497	25.4818001123908	29.34362304549707	22.974647908767164
35	22.633560588945194	26.921321274295266	27.425530220048355	23.019587916711185
36	23.805997275325108	26.070397312625587	26.828233199763908	23.295372212285397
37	21.386359824013514	28.508766563981602	28.154112962269696	21.950760649735184
38	22.760795457171465	26.849112767883092	26.731665197212685	23.658426577732754
39	22.06945881953773	25.311582695731676	26.164899107915012	26.454059376815575
40	24.347262017102214	24.409003552517145	27.60811769334059	23.635616737040056
41	22.454616165298756	24.08077565420608	27.167308686199394	26.29729949429577
42	22.30011279860433	23.756816105106875	29.442828181000845	24.50024291528795
43	21.68538895128291	23.836174776122366	30.098495556430976	24.379940716163752
44	22.21093964309532	23.72239744203541	28.630319362237945	25.436343552631328
45	21.84280132030644	23.606934517813833	27.498146530525357	27.052117631354367
46	22.845238085528475	23.722288694284792	28.295022860136527	25.137450360050206
47	20.17575267716572	25.303589736061056	30.598354592159232	23.922302994613993
48	20.737924173999662	25.560397549151944	29.21016236854776	24.491515908300634
49	23.1145247030031	23.123958570369446	29.574766389441464	24.186750337185995
50	21.140916150862495	23.32283101931713	29.193469588827476	26.3427832409929
51	20.76698701035306	23.68732629246022	27.310665408455193	28.23502128873153
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1242.0
1	2402.5
2	3563.0
3	7765.5
4	11968.0
5	8364.0
6	4760.0
7	4882.0
8	5004.0
9	5269.0
10	5534.0
11	5750.0
12	5966.0
13	6494.5
14	7023.0
15	6444.0
16	5865.0
17	6240.5
18	6616.0
19	6695.0
20	6774.0
21	7826.5
22	8879.0
23	10950.0
24	13021.0
25	15996.5
26	25353.5
27	31735.0
28	35099.0
29	38463.0
30	45604.0
31	52745.0
32	61032.5
33	69320.0
34	82385.5
35	95451.0
36	103736.5
37	112022.0
38	121823.5
39	131625.0
40	145691.5
41	159758.0
42	175222.0
43	190686.0
44	217124.5
45	243563.0
46	276233.0
47	308903.0
48	345754.0
49	382605.0
50	381445.5
51	380286.0
52	331408.5
53	282531.0
54	255038.5
55	227546.0
56	211860.0
57	196174.0
58	180616.0
59	165058.0
60	150208.5
61	135359.0
62	122351.5
63	109344.0
64	96525.5
65	83707.0
66	71276.0
67	58845.0
68	49592.5
69	40340.0
70	35058.5
71	29777.0
72	24118.5
73	18460.0
74	15598.5
75	9873.0
76	7009.0
77	5875.0
78	4741.0
79	3509.0
80	2277.0
81	1739.5
82	1202.0
83	813.5
84	425.0
85	312.5
86	200.0
87	158.5
88	117.0
89	71.0
90	25.0
91	16.0
92	7.0
93	4.5
94	2.0
95	2.0
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3678237.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.6478901331749
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.86669652676717	13.13789506926269
2	12.69631447047378	4.989115836242149
3	5.550438361183483	3.2716320933447722
4	3.0578590117799265	2.4032191162476306
5	1.9631834562301114	1.9286206429637898
6	1.232682095489602	1.4531761428786907
7	0.9118047902124221	1.254052823869738
8	0.6817066265166767	1.0715277520685567
9	0.5386687388056886	0.9525333778407049
>10	3.9854062534536876	16.908383994410308
>50	1.4369796143484852	20.837678285295862
>100	1.0665678439009254	28.48846524215926
>500	0.007325240525029382	1.0154499068705496
>1k	0.003803490272611406	1.4305089671472266
>5k	5.634800403868749E-4	0.8577407493981223
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	8767	0.2383478824230195	Illumina PCR Primer Index 11 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGC	8354	0.2271196771714275	Illumina PCR Primer Index 11 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTGCT	7401	0.20121052558603483	Illumina PCR Primer Index 11 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6468	0.17584511275374587	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTACAGCTCGTATGCCGTCTTCTG	4100	0.1114664443862644	Illumina PCR Primer Index 11 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.359346882759322E-4	0.0	0.0	0.21303684346604093	0.0
2	1.359346882759322E-4	0.0	0.0	0.8730813158586573	0.0
3	1.359346882759322E-4	0.0	0.0	1.3426541030390375	0.0
4	1.359346882759322E-4	0.0	0.0	1.8858763043273177	0.0
5	1.359346882759322E-4	0.0	0.0	3.3214825472094374	0.0
6	1.359346882759322E-4	0.0	0.0	4.321961852920299	0.0
7	1.359346882759322E-4	0.0	0.0	5.336904609463718	0.0
8	1.359346882759322E-4	0.0	0.0	6.873020960857063	0.0
9	1.359346882759322E-4	0.0	0.0	7.5794463488894275	0.0
10	1.359346882759322E-4	0.0	0.0	8.842116481346906	0.0
11	1.359346882759322E-4	0.0	0.0	10.540647598292335	0.0
12	1.359346882759322E-4	0.0	0.0	11.787413372221529	0.0
13	1.6312162593111864E-4	0.0	0.0	12.308750088697384	0.0
14	1.6312162593111864E-4	0.0	0.0	12.560310822820824	0.0
15	1.6312162593111864E-4	0.0	0.0	12.940764828367504	0.0
16	1.9030856358630507E-4	0.0	0.0	13.62780592985172	0.0
17	1.9030856358630507E-4	0.0	0.0	14.453989778255181	0.0
18	1.9030856358630507E-4	0.0	0.0	15.346727250038537	0.0
19	2.446824388966779E-4	0.0	0.0	15.90721315673786	0.0
20	2.446824388966779E-4	0.0	0.0	16.509947564553343	0.0
21	2.446824388966779E-4	0.0	0.0	17.20411164370322	0.0
22	2.446824388966779E-4	0.0	0.0	17.941883570852013	0.0
23	2.446824388966779E-4	0.0	0.0	18.63808667032603	0.0
24	2.446824388966779E-4	0.0	0.0	19.211105755284393	0.0
25	2.446824388966779E-4	0.0	0.0	19.72056178000493	0.0
26	2.446824388966779E-4	0.0	0.0	20.21963239454119	0.0
27	2.718693765518644E-4	0.0	0.0	20.828048872326608	0.0
28	2.990563142070508E-4	0.0	0.0	21.345470669780116	0.0
29	3.2624325186223727E-4	0.0	0.0	21.913596105960547	0.0
30	3.8061712717261015E-4	0.0	0.0	22.557138107196465	0.0
31	3.8061712717261015E-4	0.0	0.0	23.051885998645545	0.0
32	3.8061712717261015E-4	0.0	0.0	23.54821073247863	0.0
33	3.8061712717261015E-4	0.0	0.0	24.06731812006676	0.0
34	3.8061712717261015E-4	0.0	0.0	24.596729357026206	0.0
35	4.0780406482779656E-4	0.0	0.0	25.172793379001952	0.0
36	4.0780406482779656E-4	0.0	0.0	25.664605081184273	0.0
37	4.6217794013816944E-4	0.0	0.0	26.196300020906754	0.0
38	4.6217794013816944E-4	0.0	0.0	26.813796935869004	0.0
39	4.893648777933559E-4	0.0	0.0	27.73524381381624	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGCGTA	20	7.035312E-4	45.000004	1
TCGATAG	155	0.0	39.193546	1
CGTTTTT	4010	0.0	38.77182	1
CCCGATA	35	6.2520394E-6	38.571426	20
GTTTGCG	250	0.0	37.800003	1
TATTGCG	235	0.0	37.340427	1
TACGGGA	1320	0.0	37.159092	4
CGGTCTA	190	0.0	36.710526	31
GCGCGTA	25	0.0021077099	36.0	3
CGACGGT	195	0.0	35.76923	28
AACGAGC	780	0.0	34.615383	15
AAATGCG	170	0.0	34.411762	1
GCGTTAG	275	0.0	34.363636	1
TCACGAC	210	0.0	34.285713	25
TATAGCG	105	0.0	34.285713	1
TTACACG	1450	0.0	34.13793	34
AGGGATC	2625	0.0	33.771427	6
GCTATCG	80	3.6379788E-12	33.750004	1
CCGTCGA	40	1.561497E-5	33.750004	22
ACACGCG	1465	0.0	33.63481	36
>>END_MODULE