SRR2935014.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935014.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2159709
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTC	11371	0.526506117259316	TruSeq Adapter, Index 23 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGC	11347	0.5253948564366774	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCG	10474	0.4849727440131981	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	8090	0.3745875022977632	TruSeq Adapter, Index 23 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	6366	0.29476193320489014	TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCT	4408	0.20410157109129057	TruSeq Adapter, Index 20 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	2517	0.11654347877422375	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCTCTTGT	2461	0.11395053685473366	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCT	2388	0.11057045185254123	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAT	20	7.034664E-4	45.000004	27
TAGCGTC	30	2.1663927E-6	45.000004	31
CGCATAT	20	7.034664E-4	45.000004	20
CGCGATC	20	7.034664E-4	45.000004	44
ATATCCG	45	3.8562575E-10	45.000004	1
TACGATA	20	7.034664E-4	45.000004	17
TTACGTA	20	7.034664E-4	45.000004	1
GTATACG	50	2.1827873E-11	45.000004	1
TCGCACT	35	1.2125929E-7	45.0	1
ACGGGTA	165	0.0	40.90909	5
TATCGAG	45	1.929584E-8	40.000004	1
GTTATCG	90	0.0	40.000004	1
TACCGGT	80	0.0	39.375004	40
AAATCGA	35	6.2508407E-6	38.571426	1
CAAACGC	35	6.2508407E-6	38.571426	15
ATTACGA	35	6.2508407E-6	38.571426	21
GCCGAAT	35	6.2508407E-6	38.571426	29
TCGCTAC	35	6.2508407E-6	38.571426	27
TTACGCG	35	6.2508407E-6	38.571426	1
CTAGCGT	35	6.2508407E-6	38.571426	30