##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935014.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2159709
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.29976816321088	33.0	31.0	34.0	30.0	34.0
2	32.47148296367705	34.0	31.0	34.0	31.0	34.0
3	32.48000818628806	34.0	31.0	34.0	30.0	34.0
4	35.99688985877264	37.0	35.0	37.0	35.0	37.0
5	35.91654107104244	37.0	35.0	37.0	35.0	37.0
6	35.85363213284753	37.0	35.0	37.0	35.0	37.0
7	36.11383941077247	37.0	35.0	37.0	35.0	37.0
8	35.83112308185964	37.0	35.0	37.0	35.0	37.0
9	37.605764943332645	39.0	38.0	39.0	35.0	39.0
10	37.419032378899196	39.0	37.0	39.0	35.0	39.0
11	37.344158865847206	39.0	37.0	39.0	34.0	39.0
12	37.16167826313637	39.0	37.0	39.0	34.0	39.0
13	37.043088675372466	39.0	37.0	39.0	33.0	39.0
14	38.07613757223774	40.0	37.0	41.0	33.0	41.0
15	38.168953317321915	40.0	37.0	41.0	33.0	41.0
16	38.29524857284014	40.0	37.0	41.0	34.0	41.0
17	38.29800449968028	40.0	37.0	41.0	34.0	41.0
18	38.31033254943143	40.0	37.0	41.0	34.0	41.0
19	38.27304187740108	40.0	37.0	41.0	34.0	41.0
20	38.18710622588506	40.0	37.0	41.0	34.0	41.0
21	38.14184735073105	40.0	37.0	41.0	34.0	41.0
22	38.121001486774375	40.0	36.0	41.0	34.0	41.0
23	37.89360649976455	40.0	36.0	41.0	33.0	41.0
24	37.68200067694305	40.0	36.0	41.0	33.0	41.0
25	37.65639954271617	40.0	35.0	41.0	33.0	41.0
26	37.80415463379557	40.0	36.0	41.0	33.0	41.0
27	37.808115352577595	40.0	36.0	41.0	33.0	41.0
28	37.78125293731702	40.0	36.0	41.0	33.0	41.0
29	37.81421848962059	40.0	36.0	41.0	34.0	41.0
30	37.68899282264416	40.0	36.0	41.0	33.0	41.0
31	37.635643042650656	40.0	36.0	41.0	33.0	41.0
32	37.53841373999923	40.0	35.0	41.0	33.0	41.0
33	37.45669347120376	40.0	35.0	41.0	33.0	41.0
34	37.30108269215899	40.0	35.0	41.0	33.0	41.0
35	37.197464565828085	40.0	35.0	41.0	32.0	41.0
36	37.112959662621215	39.0	35.0	41.0	32.0	41.0
37	37.029984132121506	39.0	35.0	41.0	32.0	41.0
38	36.98416082907466	39.0	35.0	41.0	32.0	41.0
39	36.92109862949129	39.0	35.0	41.0	32.0	41.0
40	36.84255239942048	39.0	35.0	41.0	31.0	41.0
41	36.73626030173509	39.0	35.0	41.0	31.0	41.0
42	36.72544495577876	39.0	35.0	41.0	31.0	41.0
43	36.6154329124896	39.0	35.0	41.0	31.0	41.0
44	36.50375166283976	39.0	35.0	41.0	31.0	41.0
45	36.473349881859086	39.0	35.0	41.0	31.0	41.0
46	36.36788011718245	38.0	35.0	41.0	31.0	41.0
47	36.266782700817565	38.0	35.0	40.0	31.0	41.0
48	36.19961207736783	38.0	35.0	40.0	31.0	41.0
49	36.14190291377218	38.0	35.0	40.0	31.0	41.0
50	36.02421854055338	38.0	35.0	40.0	31.0	41.0
51	34.98205962006919	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	20.0
9	36.0
10	39.0
11	27.0
12	32.0
13	43.0
14	75.0
15	85.0
16	172.0
17	365.0
18	589.0
19	1048.0
20	1968.0
21	3280.0
22	4864.0
23	6951.0
24	9176.0
25	11510.0
26	13649.0
27	16333.0
28	19382.0
29	23578.0
30	29325.0
31	38176.0
32	51296.0
33	74489.0
34	144399.0
35	174505.0
36	158115.0
37	246985.0
38	440273.0
39	688338.0
40	583.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.75621900913503	20.579392871910056	24.591831584718125	11.072556534236789
2	30.75020755110989	26.612890903357812	24.803758284102166	17.83314326143013
3	31.117525555526232	26.915940990198216	25.092593492919647	16.873939961355905
4	27.34562850828514	25.497138734894374	28.163933196555647	18.99329956026483
5	26.093700586514203	29.210555681344108	24.356892525798614	20.338851206343076
6	26.440923291054492	34.2621621709221	23.934891228401604	15.362023309621806
7	79.9430849248672	5.566953696076647	9.736450605150972	4.753510773905188
8	80.37129076185727	5.751422992634656	7.964684131056544	5.912602114451531
9	75.03149729894166	6.3547450142588655	9.727838333775523	8.885919353023949
10	42.269490936047404	23.386761827635112	17.424291883767673	16.91945535254981
11	32.00162614500379	22.889565214572887	23.808439007292186	21.30036963313113
12	27.329931949165378	21.477013801396392	29.227131988615135	21.9659222608231
13	24.82468702959519	23.942670054160075	32.001996565278006	19.230646350966726
14	20.9310606197409	26.720173875276714	30.51786143410987	21.83090407087251
15	18.40553519015756	23.53914346793943	35.85473783736605	22.20058350453695
16	22.598924206918618	24.83959644563226	29.182542648106757	23.378936699342365
17	23.1941895875787	23.48608076365844	28.277096590327677	25.042633058435186
18	24.34957672538291	22.87359084024746	30.026267427695117	22.75056500667451
19	23.95554215868897	25.541403957662816	26.408187399320926	24.094866484327287
20	24.88626940018308	26.831855587951896	27.372206162959916	20.90966884890511
21	22.964204899826782	27.46694114808986	29.314504870795094	20.254349081288268
22	22.52678485851566	23.10524241923333	28.53870590899052	25.82926681326049
23	21.401864788265456	26.361977470112873	28.47027076332969	23.765886978291984
24	22.414686423031995	24.454637175656536	28.6505728318028	24.48010356950867
25	22.308977737278493	27.202090652027657	26.40953017281495	24.0794014378789
26	21.09381402772318	25.41161795408548	27.878153955000418	25.616414063190923
27	21.97754419692653	25.653594998215034	28.39382527923901	23.975035525619422
28	18.966860813192888	24.970493710032233	29.071370263308623	26.99127521346626
29	23.331106181434627	23.646796860132547	28.060447032447428	24.961649925985398
30	23.06921904756613	23.91502744119694	29.625750506202458	23.390003005034472
31	24.105006739333863	24.477186509849243	25.88144976939023	25.536356981426668
32	24.58919234026436	24.4147243911101	28.035999294349377	22.960083974276163
33	23.91789819832209	22.65981203949236	26.77407002517469	26.648219737010866
34	22.162615426430136	22.611518496241857	30.619819614586962	24.606046462741045
35	22.18275702884046	23.81881077497015	30.558376151601905	23.440056044587486
36	25.64387146601695	24.151401878679028	27.313448246962903	22.891278408341122
37	21.352830404466527	24.6126677251426	30.889346666611107	23.14515520377977
38	21.607448040453598	24.839827958303644	27.85231714087407	25.70040686036869
39	23.319252732659816	22.248877047787456	29.065443538921215	25.36642668063151
40	23.665132663706082	21.053484520368254	31.706540094058965	23.574842721866695
41	22.52391410139051	22.47724114683969	29.295243016536027	25.70360173523377
42	23.68745048522741	21.77043296110726	31.377190167749454	23.16492638591588
43	24.413983550561674	21.426914459309103	29.4932326531028	24.665869337026425
44	22.40213843624303	21.891838205980527	29.06294320207028	26.643080155706162
45	21.582768789684163	21.768812372407577	28.64617409104653	28.00224474686173
46	23.694858890711664	22.570540753407055	28.985201247019855	24.749399108861425
47	20.17947788336299	23.713565114559415	32.75862627789207	23.348330724185526
48	21.898968796259126	23.636425092454587	29.360761102537424	25.103845008748866
49	23.36916686461	20.723625266181696	31.67621193410779	24.230995935100516
50	21.788490949475136	21.917952835312533	30.441693765224855	25.851862449987472
51	21.46122463720807	21.326854682737352	28.116334191319293	29.095586488735286
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	973.0
1	1690.0
2	2407.0
3	3550.5
4	4694.0
5	3685.0
6	2676.0
7	2732.0
8	2788.0
9	2985.5
10	3183.0
11	3568.5
12	3954.0
13	4196.0
14	4438.0
15	4025.0
16	3612.0
17	3613.5
18	3615.0
19	4067.5
20	4520.0
21	4977.5
22	5435.0
23	5938.0
24	6441.0
25	7764.0
26	11458.5
27	13830.0
28	15830.5
29	17831.0
30	22308.5
31	26786.0
32	30784.0
33	34782.0
34	39873.0
35	44964.0
36	51517.0
37	58070.0
38	62084.0
39	66098.0
40	75599.0
41	85100.0
42	95285.0
43	105470.0
44	119640.0
45	133810.0
46	174701.5
47	215593.0
48	222656.0
49	229719.0
50	226611.5
51	223504.0
52	197142.0
53	170780.0
54	153536.5
55	136293.0
56	124269.5
57	112246.0
58	104756.0
59	97266.0
60	89798.0
61	82330.0
62	74360.5
63	66391.0
64	60404.0
65	54417.0
66	46415.0
67	38413.0
68	34746.0
69	31079.0
70	26291.0
71	21503.0
72	17807.0
73	14111.0
74	11754.0
75	7462.5
76	5528.0
77	4498.0
78	3468.0
79	2436.0
80	1404.0
81	1126.0
82	848.0
83	695.0
84	542.0
85	374.5
86	207.0
87	124.5
88	42.0
89	32.5
90	23.0
91	26.5
92	30.0
93	19.0
94	8.0
95	4.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2159709.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.943316397056574
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.90548068033856	13.343171699051476
2	12.543028881873482	5.002991871372433
3	5.628981355798947	3.3678166851549265
4	3.145516315073895	2.509281084144887
5	2.000798470873665	1.995127847569024
6	1.3554580542327974	1.6219397303102006
7	0.9504468934049836	1.3268544179642563
8	0.6918743399350182	1.1038615094662994
9	0.5365015750099115	0.9629658592147659
>10	3.856937774175664	15.99938823464063
>50	1.353620076131322	20.20010543998816
>100	1.0146296281202285	27.29593266650111
>500	0.0110721110771739	1.4443155054775385
>1k	0.004475959797155407	1.5876570775031702
>5k	4.7115366285846384E-4	0.6791693756809347
>10k+	7.067304942876958E-4	1.5594209959602647
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTC	11371	0.526506117259316	TruSeq Adapter, Index 23 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGC	11347	0.5253948564366774	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCG	10474	0.4849727440131981	No Hit
GCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	8090	0.3745875022977632	TruSeq Adapter, Index 23 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	6366	0.29476193320489014	TruSeq Adapter, Index 23 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGCT	4408	0.20410157109129057	TruSeq Adapter, Index 20 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCTTCTGC	2517	0.11654347877422375	TruSeq Adapter, Index 23 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTCTCTTGT	2461	0.11395053685473366	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTCTCTTGTCGTATGCCGTCT	2388	0.11057045185254123	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3890760282982568E-4	0.0	0.0	0.21502896918057016	0.0
2	1.3890760282982568E-4	0.0	0.0	1.1300596515549086	0.0
3	1.3890760282982568E-4	0.0	0.0	1.605771888712785	0.0
4	1.3890760282982568E-4	0.0	0.0	2.252664595091283	0.0
5	1.3890760282982568E-4	0.0	0.0	4.3477616660392675	0.0
6	1.3890760282982568E-4	0.0	0.0	5.3234023657816865	0.0
7	1.3890760282982568E-4	0.0	0.0	6.381924601879235	0.0
8	1.8521013710643425E-4	0.0	0.0	7.868837885103965	0.0
9	1.8521013710643425E-4	0.0	0.0	8.419097202447182	0.0
10	1.8521013710643425E-4	0.0	0.0	10.170166443719964	0.0
11	1.8521013710643425E-4	0.0	0.0	11.96939032064042	0.0
12	3.2411773993625996E-4	0.0	0.0	13.904882555936934	0.0
13	3.2411773993625996E-4	0.0	0.0	14.460698177393343	0.0
14	3.2411773993625996E-4	0.0	0.0	14.68410790527798	0.0
15	3.2411773993625996E-4	0.0	0.0	15.152458039485875	0.0
16	3.704202742128685E-4	0.0	0.0	15.998405340719513	0.0
17	3.704202742128685E-4	0.0	0.0	16.957377128122353	0.0
18	3.704202742128685E-4	0.0	0.0	18.050487357324528	0.0
19	3.704202742128685E-4	0.0	0.0	18.809941524529464	0.0
20	3.704202742128685E-4	0.0	0.0	19.42965464328759	0.0
21	3.704202742128685E-4	0.0	0.0	20.174755024866776	0.0
22	3.704202742128685E-4	0.0	0.0	20.976576010934807	0.0
23	3.704202742128685E-4	0.0	0.0	21.750013543491274	0.0
24	4.1672280848947704E-4	0.0	0.0	22.37852414376196	0.0
25	4.1672280848947704E-4	0.0	0.0	22.929246486447944	0.0
26	4.1672280848947704E-4	0.0	0.0	23.463716639602836	0.0
27	4.1672280848947704E-4	0.0	0.0	24.04648033600823	0.0
28	4.1672280848947704E-4	0.0	0.0	24.54742745434686	0.0
29	4.1672280848947704E-4	0.0	0.0	25.119124845060146	0.0
30	4.1672280848947704E-4	0.0	0.0	25.769212426303728	0.0
31	4.1672280848947704E-4	0.0	0.0	26.299793166579388	0.0
32	4.1672280848947704E-4	0.0	0.0	26.846718701454687	0.0
33	4.1672280848947704E-4	0.0	0.0	27.351647837741105	0.0
34	4.1672280848947704E-4	0.0	0.0	27.878709585411737	0.0
35	4.6302534276608564E-4	0.0	0.0	28.46207521476273	0.0
36	4.6302534276608564E-4	0.0	0.0	28.947881404392906	0.0
37	4.6302534276608564E-4	0.0	0.0	29.455496087667367	0.0
38	5.093278770426942E-4	0.0	0.0	30.013210113029118	0.0
39	5.093278770426942E-4	0.0	0.0	30.817901856222296	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGAT	20	7.034664E-4	45.000004	27
TAGCGTC	30	2.1663927E-6	45.000004	31
CGCATAT	20	7.034664E-4	45.000004	20
CGCGATC	20	7.034664E-4	45.000004	44
ATATCCG	45	3.8562575E-10	45.000004	1
TACGATA	20	7.034664E-4	45.000004	17
TTACGTA	20	7.034664E-4	45.000004	1
GTATACG	50	2.1827873E-11	45.000004	1
TCGCACT	35	1.2125929E-7	45.0	1
ACGGGTA	165	0.0	40.90909	5
TATCGAG	45	1.929584E-8	40.000004	1
GTTATCG	90	0.0	40.000004	1
TACCGGT	80	0.0	39.375004	40
AAATCGA	35	6.2508407E-6	38.571426	1
CAAACGC	35	6.2508407E-6	38.571426	15
ATTACGA	35	6.2508407E-6	38.571426	21
GCCGAAT	35	6.2508407E-6	38.571426	29
TCGCTAC	35	6.2508407E-6	38.571426	27
TTACGCG	35	6.2508407E-6	38.571426	1
CTAGCGT	35	6.2508407E-6	38.571426	30
>>END_MODULE