FastQCFastQC Report
Sat 14 Jan 2017
SRR2935013.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935013.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1742652
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC443522.5450864544384078No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG410172.3537114696451154No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC378782.1735837103449227No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC104160.5977096976332624TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCGTCTAT99680.572001753649036No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCT88000.5049774711187316No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTA62800.36037028620745853No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTC60060.34464712403853437No Hit
GAACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT58500.33569525068688416No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC51480.29541182060445803No Hit
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC40590.232920858553515TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATCCCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG40300.2312567282509646No Hit
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT33850.19424417497010305TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20240.11614481835730828No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATG19580.1123574873239178No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTT19460.11166888168148317No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGCGTCTA18660.1070781773985856No Hit
TCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC17890.1026596245262967TruSeq Adapter, Index 16 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG302.166149E-645.0000041
AACGGTA207.0342893E-445.00000444
CGTAGTC253.8916372E-545.00000418
CGCTAGC207.0342893E-445.00000421
CGATCGC253.8916372E-545.00000434
TGTCGAT351.212411E-745.00000418
GCGTAAA207.0342893E-445.0000041
TCGCGGA207.0342893E-445.00000441
GTCGGTA207.0342893E-445.00000445
TTAACGG1250.044.9999962
AACGTAG1050.042.8571431
CGATGAA28600.042.48251719
TGATCCG12100.042.2107474
GATGAAT30000.042.14999820
GATCCGG12250.041.8775525
AATGATC25050.041.7664682
ATCGAAT4150.041.74698643
CCGATGA29150.041.2950318
TTTACGG1800.041.2500042
TGAATGA29800.041.2248322
Produced by FastQC (version 0.11.5)