##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935013.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1742652
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.20531637986242	33.0	31.0	34.0	30.0	34.0
2	32.37772486991092	34.0	31.0	34.0	30.0	34.0
3	32.40363882174984	34.0	31.0	34.0	30.0	34.0
4	35.94378625221788	37.0	35.0	37.0	35.0	37.0
5	35.83604242269828	37.0	35.0	37.0	35.0	37.0
6	35.73528392358314	37.0	35.0	37.0	35.0	37.0
7	35.957864220739424	37.0	35.0	37.0	35.0	37.0
8	35.409727243305035	37.0	35.0	37.0	33.0	37.0
9	37.351560150850545	39.0	37.0	39.0	34.0	39.0
10	37.28997700057154	39.0	37.0	39.0	34.0	39.0
11	37.21031049228417	39.0	37.0	39.0	34.0	39.0
12	36.93023736236495	39.0	35.0	39.0	33.0	39.0
13	36.70653062114524	39.0	35.0	39.0	33.0	39.0
14	37.651739991690825	40.0	36.0	41.0	32.0	41.0
15	37.67424018105738	40.0	36.0	41.0	32.0	41.0
16	37.98257999876051	40.0	36.0	41.0	33.0	41.0
17	37.8699792041096	40.0	36.0	41.0	33.0	41.0
18	37.94166936370543	40.0	36.0	41.0	33.0	41.0
19	37.90878327973686	40.0	36.0	41.0	33.0	41.0
20	37.63250149771727	40.0	35.0	41.0	32.0	41.0
21	37.78919141630113	40.0	36.0	41.0	33.0	41.0
22	37.77049405159492	40.0	35.0	41.0	33.0	41.0
23	37.4506637010717	39.0	35.0	41.0	33.0	41.0
24	37.2784531851454	39.0	35.0	41.0	32.0	41.0
25	37.31440069503263	39.0	35.0	41.0	32.0	41.0
26	37.405857853432586	39.0	35.0	41.0	33.0	41.0
27	37.359398204575555	39.0	35.0	41.0	33.0	41.0
28	37.34038465511186	39.0	35.0	41.0	33.0	41.0
29	37.32525139844329	39.0	35.0	41.0	33.0	41.0
30	37.272585117395785	39.0	35.0	41.0	32.0	41.0
31	37.28354771922334	39.0	35.0	41.0	32.0	41.0
32	37.03220608589667	39.0	35.0	41.0	32.0	41.0
33	36.88217326236104	39.0	35.0	41.0	31.0	41.0
34	36.698732736082704	39.0	35.0	41.0	31.0	41.0
35	36.655228926945824	39.0	35.0	41.0	31.0	41.0
36	36.5998036326243	39.0	35.0	41.0	31.0	41.0
37	36.504917218125016	39.0	35.0	41.0	31.0	41.0
38	36.42514053293486	39.0	35.0	41.0	30.0	41.0
39	36.32986448240957	39.0	35.0	41.0	30.0	41.0
40	36.16221827421654	39.0	35.0	41.0	30.0	41.0
41	36.10976029637587	38.0	35.0	41.0	30.0	41.0
42	36.14544039773862	39.0	35.0	41.0	30.0	41.0
43	36.06457743714752	38.0	35.0	40.0	30.0	41.0
44	36.08484941342276	38.0	35.0	40.0	30.0	41.0
45	36.08291959610984	38.0	35.0	40.0	30.0	41.0
46	35.98058419007352	38.0	35.0	40.0	30.0	41.0
47	35.6903673252032	38.0	35.0	40.0	29.0	41.0
48	35.681872226927695	38.0	35.0	40.0	29.0	41.0
49	35.72166445165185	38.0	35.0	40.0	29.0	41.0
50	35.60044403587176	37.0	35.0	40.0	29.0	41.0
51	34.519205785205536	36.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	17.0
10	27.0
11	18.0
12	16.0
13	31.0
14	25.0
15	64.0
16	130.0
17	247.0
18	509.0
19	1061.0
20	2247.0
21	4676.0
22	8124.0
23	10638.0
24	12132.0
25	13155.0
26	14088.0
27	14913.0
28	16715.0
29	20199.0
30	26182.0
31	35022.0
32	48564.0
33	70945.0
34	130609.0
35	149602.0
36	131102.0
37	199063.0
38	335950.0
39	496171.0
40	401.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.804608148959176	18.044337021964225	22.46254559143191	9.688509237644693
2	27.08251561413294	34.55692817613614	22.63354932596984	15.72700688376107
3	27.695259868292695	34.61632041279613	23.047343933269524	14.641075785641656
4	24.50351533180463	22.036241314961337	36.08333735019958	17.376906003034456
5	33.27089975508593	25.826039851903882	21.97042209230529	18.9326383007049
6	23.897771901676297	39.435527001374915	22.59579078324301	14.07091031370578
7	72.53077493383647	5.324470978715199	17.48306603957646	4.661688047871864
8	71.98207100442315	12.491076818550118	9.101874614093921	6.424977562932817
9	67.67139968278232	5.8051751009381105	9.134927684930783	17.388497531348772
10	46.70559583898564	19.72671537403911	17.325260579851857	16.242428207123396
11	36.68512129788392	19.943683535209555	23.087914282369628	20.2832808845369
12	28.706649405618563	18.267617401523655	28.513323371505038	24.512409821352744
13	26.459499659140207	21.116895398507562	35.14786658495213	17.2757383574001
14	21.274987777249844	28.71870000436117	28.732644268620472	21.273667949768512
15	17.037767724135396	21.152071670075266	39.40970428978361	22.40045631600572
16	21.276824058963008	22.809717602826037	26.984389310086005	28.92906902812495
17	22.52388887741213	24.091270087200428	29.280315289570147	24.104525745817295
18	22.609735047502312	22.49261470448489	29.31313882519287	25.584511422819933
19	22.88603806152921	24.60910153031127	26.404066904924218	26.1007935032353
20	24.13356195040662	26.52537626559979	30.770113596977485	18.57094818701611
21	21.2826772069237	30.374509655398786	29.649235762504507	18.693577375173014
22	20.720832386500575	20.84202697956907	31.697780164944007	26.73936046898635
23	20.563371229597188	27.972767942193848	29.37350658651297	22.09035424169599
24	22.60611986787953	24.99041690480945	26.130920000091812	26.272543227219202
25	20.312374472929765	32.779464861601745	24.2079887435931	22.700171921875395
26	19.98017963425859	23.699568244262192	28.979968461861578	27.340283659617644
27	23.97403497657593	25.449315181688597	25.985968512359324	24.590681329376146
28	17.818646522656273	25.720797956218455	29.991243231580373	26.4693122895449
29	23.38143243745739	25.028519750357503	26.61845279493554	24.97159501724957
30	24.49708834580857	26.871457984726728	27.68860334708249	20.94285032238221
31	24.840071339544558	22.6181130828186	22.81132434932505	29.730491228311788
32	30.612595056270557	26.058329488618497	23.014577781450342	20.314497673660608
33	24.84701477977244	21.955617070992947	24.982899626546207	28.214468522688406
34	23.39772943766168	24.6891519362443	25.595184810277672	26.317933815816353
35	24.615872819128544	24.279948033227516	27.149941583288	23.954237564355935
36	23.0237018062126	29.936154780185603	23.19063129069946	23.849512122902336
37	26.76518318057765	23.46205668142578	27.483456249440508	22.289303888556063
38	21.945460137767036	23.94534307480782	26.222160247714406	27.887036539710742
39	25.939315480084378	23.47858321684421	24.459961024920638	26.122140278150773
40	22.454913545561592	20.622820850060712	33.06667079830052	23.855594806077175
41	20.59510447295272	25.75270335098459	24.18509260598215	29.467099570080546
42	21.10145915535632	21.635817133885595	33.599594181741395	23.663129529016693
43	22.91788607249181	25.638222662929834	26.08277498892493	25.36111627565343
44	24.364589143443443	20.42203492148748	28.519119135662198	26.69425679940688
45	20.739826425471065	19.918664196867763	27.821848538893594	31.519660838767578
46	27.44627154474904	24.00944078335778	25.350901958623982	23.1933857132692
47	18.45331138976686	22.655183019903	36.10290522720543	22.788600363124708
48	21.967839821146164	24.81539630402398	26.31833550244111	26.89842837238875
49	22.112561773664506	19.86495295675786	34.17343221710359	23.849053052474044
50	22.52371672600152	21.986604324902505	28.438437507890274	27.051241441205704
51	21.528337269862256	21.991481948203084	25.33167838443935	31.14850239749531
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	512.0
1	1138.0
2	1764.0
3	2932.5
4	4101.0
5	2989.0
6	1877.0
7	1952.5
8	2028.0
9	2206.0
10	2384.0
11	2675.5
12	2967.0
13	2904.0
14	2841.0
15	2643.0
16	2445.0
17	2647.0
18	2849.0
19	2742.5
20	2636.0
21	3034.5
22	3433.0
23	3765.0
24	4097.0
25	5501.5
26	7944.0
27	8982.0
28	11190.5
29	13399.0
30	16301.5
31	19204.0
32	22484.0
33	25764.0
34	27877.5
35	29991.0
36	33455.5
37	36920.0
38	41427.0
39	45934.0
40	52585.5
41	59237.0
42	66446.5
43	73656.0
44	84317.0
45	94978.0
46	184446.5
47	273915.0
48	227703.0
49	181491.0
50	173554.5
51	165618.0
52	147128.0
53	128638.0
54	117605.0
55	106572.0
56	102319.5
57	98067.0
58	89196.0
59	80325.0
60	73571.0
61	66817.0
62	60482.0
63	54147.0
64	47895.0
65	41643.0
66	36907.5
67	32172.0
68	26259.0
69	20346.0
70	19159.0
71	17972.0
72	14383.5
73	10795.0
74	9189.5
75	5667.0
76	3750.0
77	2937.5
78	2125.0
79	1568.0
80	1011.0
81	716.5
82	422.0
83	299.5
84	177.0
85	151.0
86	125.0
87	70.5
88	16.0
89	16.0
90	16.0
91	9.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1742652.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.307615496655487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.1851002913022	14.049844148133129
2	12.017461432392839	4.88091972029841
3	5.302119439944749	3.2302020871122092
4	2.7661363521596143	2.2469453380391466
5	1.6644976087189602	1.690098871648358
6	1.109500491165819	1.3518785620767517
7	0.7987749098167345	1.1354849615853737
8	0.6140290883556927	0.9975573304071444
9	0.4520630045494589	0.826228950898791
>10	3.829096255014407	17.41377716237314
>50	1.7245524398690406	25.609815815458433
>100	0.5241016711747344	13.84522199798634
>500	0.005426665799474467	0.7551781079732663
>1k	0.004284209841690369	1.7751325648634269
>5k	0.0017136839366761476	2.4390744851376187
>10k+	0.0011424559577840985	7.752639896008502
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC	44352	2.5450864544384078	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG	41017	2.3537114696451154	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC	37878	2.1735837103449227	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	10416	0.5977096976332624	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCGTCTAT	9968	0.572001753649036	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCT	8800	0.5049774711187316	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTA	6280	0.36037028620745853	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTC	6006	0.34464712403853437	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCT	5850	0.33569525068688416	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC	5148	0.29541182060445803	No Hit
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	4059	0.232920858553515	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATCCCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG	4030	0.2312567282509646	No Hit
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT	3385	0.19424417497010305	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2024	0.11614481835730828	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATG	1958	0.1123574873239178	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTT	1946	0.11166888168148317	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAGCGTCTA	1866	0.1070781773985856	No Hit
TCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	1789	0.1026596245262967	TruSeq Adapter, Index 16 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7215141060865854E-4	0.0	0.0	0.20331081592882572	0.0
2	1.7215141060865854E-4	0.0	0.0	1.2649111813488867	0.0
3	2.2953521414487805E-4	0.0	0.0	1.6618923342124532	0.0
4	2.2953521414487805E-4	0.0	0.0	2.4469601503914724	0.0
5	2.2953521414487805E-4	0.0	0.0	5.865255943240532	0.0
6	2.2953521414487805E-4	0.0	0.0	6.630182044378338	0.0
7	2.2953521414487805E-4	0.0	0.0	7.874262905043577	0.0
8	2.2953521414487805E-4	0.0	0.0	9.223183974769489	0.0
9	2.2953521414487805E-4	0.0	0.0	9.622690014988649	0.0
10	2.2953521414487805E-4	0.0	0.0	13.143415897149861	0.0
11	2.2953521414487805E-4	0.0	0.0	14.537440636455242	0.0
12	2.2953521414487805E-4	0.0	0.0	18.556200549507302	0.0
13	2.8691901768109753E-4	0.0	0.0	19.131760098975583	0.0
14	2.8691901768109753E-4	0.0	0.0	19.41764620819303	0.0
15	2.8691901768109753E-4	0.0	0.0	20.151929358242494	0.0
16	2.8691901768109753E-4	0.0	0.0	20.897517117588595	0.0
17	2.8691901768109753E-4	0.0	0.0	21.68510982112321	0.0
18	2.8691901768109753E-4	0.0	0.0	22.514076247007434	0.0
19	2.8691901768109753E-4	0.0	0.0	23.770265090218818	0.0
20	2.8691901768109753E-4	0.0	0.0	24.422661552622095	0.0
21	2.8691901768109753E-4	0.0	0.0	25.061228518373145	0.0
22	2.8691901768109753E-4	0.0	0.0	25.788568228194727	0.0
23	3.443028212173171E-4	0.0	0.0	26.424897225607868	0.0
24	4.0168662475353656E-4	0.0	0.0	26.93785104541813	0.0
25	4.0168662475353656E-4	0.0	0.0	27.398183917385687	0.0
26	4.0168662475353656E-4	0.0	0.0	27.857024810461297	0.0
27	4.0168662475353656E-4	0.0	0.0	28.472753022404934	0.0
28	4.590704282897561E-4	0.0	0.0	28.914895228651503	0.0
29	4.590704282897561E-4	0.0	0.0	29.42543892871325	0.0
30	4.590704282897561E-4	0.0	0.0	29.982578277246404	0.0
31	4.590704282897561E-4	0.0	0.0	30.419383789764108	0.0
32	4.590704282897561E-4	0.0	0.0	30.840810442934103	0.0
33	4.590704282897561E-4	0.0	0.0	31.26217971230056	0.0
34	4.590704282897561E-4	0.0	0.0	31.733472890743535	0.0
35	4.590704282897561E-4	0.0	0.0	32.23988495695067	0.0
36	4.590704282897561E-4	0.0	0.0	32.67364912788096	0.0
37	5.164542318259756E-4	0.0	0.0	33.14907394017853	0.0
38	5.164542318259756E-4	0.0	0.0	33.70604113730108	0.0
39	5.164542318259756E-4	0.0	0.0	34.60180231050146	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGTCG	30	2.166149E-6	45.000004	1
AACGGTA	20	7.0342893E-4	45.000004	44
CGTAGTC	25	3.8916372E-5	45.000004	18
CGCTAGC	20	7.0342893E-4	45.000004	21
CGATCGC	25	3.8916372E-5	45.000004	34
TGTCGAT	35	1.212411E-7	45.000004	18
GCGTAAA	20	7.0342893E-4	45.000004	1
TCGCGGA	20	7.0342893E-4	45.000004	41
GTCGGTA	20	7.0342893E-4	45.000004	45
TTAACGG	125	0.0	44.999996	2
AACGTAG	105	0.0	42.857143	1
CGATGAA	2860	0.0	42.482517	19
TGATCCG	1210	0.0	42.210747	4
GATGAAT	3000	0.0	42.149998	20
GATCCGG	1225	0.0	41.877552	5
AATGATC	2505	0.0	41.766468	2
ATCGAAT	415	0.0	41.746986	43
CCGATGA	2915	0.0	41.29503	18
TTTACGG	180	0.0	41.250004	2
TGAATGA	2980	0.0	41.22483	22
>>END_MODULE