FastQCFastQC Report
Sat 14 Jan 2017
SRR2935012.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935012.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences686103
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC59430.8661964748733061TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC46420.6765747999935869TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT34480.5025484511800706Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC22600.3293966066319488TruSeq Adapter, Index 13 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC16940.24690170426306257Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9830.14327294881380784No Hit
AAAACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC9720.14166969099391782No Hit
CGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG9230.1345279061598623No Hit
AGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG9220.1343821554489632No Hit
ACCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG9120.13292464833997228No Hit
GGCCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCT9050.13190439336367862No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCC8740.12738612132580676No Hit
GGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG8710.1269488691931095TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG8200.11951558293725578TruSeq Adapter, Index 13 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC7440.10843852890892475No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7130.10392025687105289No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATC351.210974E-745.00000416
ACAACAG253.889155E-545.0000041
TTAGGTA253.889155E-545.00000418
ACTATCG253.889155E-545.00000419
CCTTAGA253.889155E-545.00000429
GTACATC253.889155E-545.00000444
ATTAGGA253.889155E-545.00000417
TGCTTAG700.045.00000421
ATAATCG253.889155E-545.00000440
CAGTTAT253.889155E-545.00000427
ATCAGCC253.889155E-545.00000439
ATTACCC253.889155E-545.00000416
TTCACGG253.889155E-545.0000042
TCGATTA253.889155E-545.00000441
CGACAAT351.210974E-745.00000420
ACGGGTA351.210974E-745.0000045
TCGATAC253.889155E-545.0000041
CCATGCG253.889155E-545.00000424
CACCTAA253.889155E-545.00000414
ATTCCGT253.889155E-545.00000414
Produced by FastQC (version 0.11.5)