##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935012.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	686103
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.094366297771614	33.0	31.0	34.0	30.0	34.0
2	32.27046813670834	34.0	31.0	34.0	30.0	34.0
3	32.27426057020593	34.0	31.0	34.0	30.0	34.0
4	35.85738147187813	37.0	35.0	37.0	35.0	37.0
5	35.76557309908279	37.0	35.0	37.0	35.0	37.0
6	35.701974776381974	37.0	35.0	37.0	33.0	37.0
7	35.998992862587684	37.0	35.0	37.0	35.0	37.0
8	35.7538547419265	37.0	35.0	37.0	35.0	37.0
9	37.46699985279178	39.0	37.0	39.0	35.0	39.0
10	37.30164712878387	39.0	37.0	39.0	34.0	39.0
11	37.214598974206496	39.0	37.0	39.0	34.0	39.0
12	36.95909214797195	39.0	35.0	39.0	33.0	39.0
13	36.726844220182684	39.0	35.0	39.0	33.0	39.0
14	37.747673454277276	40.0	36.0	41.0	33.0	41.0
15	37.89676185645595	40.0	36.0	41.0	33.0	41.0
16	38.00805272677717	40.0	36.0	41.0	34.0	41.0
17	38.05182020775306	40.0	36.0	41.0	34.0	41.0
18	38.051461661004254	40.0	36.0	41.0	34.0	41.0
19	38.01489572265389	40.0	36.0	41.0	34.0	41.0
20	37.96518307018042	40.0	36.0	41.0	34.0	41.0
21	37.858815658873375	40.0	35.0	41.0	34.0	41.0
22	37.74834828006873	39.0	35.0	41.0	33.0	41.0
23	37.537757450411966	39.0	35.0	41.0	33.0	41.0
24	37.291996974215245	39.0	35.0	41.0	33.0	41.0
25	37.252427113713246	39.0	35.0	41.0	33.0	41.0
26	37.45105764003364	39.0	35.0	41.0	33.0	41.0
27	37.505139898819856	39.0	35.0	41.0	33.0	41.0
28	37.508476132592335	39.0	35.0	41.0	33.0	41.0
29	37.60129018529288	39.0	35.0	41.0	33.0	41.0
30	37.48686567468733	39.0	35.0	41.0	33.0	41.0
31	37.362694814043955	39.0	35.0	41.0	33.0	41.0
32	37.29102918949487	39.0	35.0	41.0	33.0	41.0
33	37.20677507604543	39.0	35.0	41.0	33.0	41.0
34	37.142914402064996	39.0	35.0	41.0	33.0	41.0
35	37.09036398325033	39.0	35.0	41.0	33.0	41.0
36	37.00139774931752	39.0	35.0	41.0	32.0	41.0
37	36.89634355191568	39.0	35.0	41.0	32.0	41.0
38	36.81596932239037	39.0	35.0	41.0	32.0	41.0
39	36.7605009743435	39.0	35.0	41.0	31.0	41.0
40	36.707822294903245	39.0	35.0	41.0	31.0	41.0
41	36.66058886202217	39.0	35.0	41.0	31.0	41.0
42	36.64326930504603	39.0	35.0	41.0	31.0	41.0
43	36.53029647152104	39.0	35.0	41.0	31.0	41.0
44	36.35677004764591	39.0	35.0	40.0	31.0	41.0
45	36.34043722298256	39.0	35.0	40.0	31.0	41.0
46	36.18357010536319	38.0	35.0	40.0	31.0	41.0
47	36.12576974594194	38.0	35.0	40.0	31.0	41.0
48	36.10599574699425	38.0	35.0	40.0	31.0	41.0
49	36.074736592027726	38.0	35.0	40.0	31.0	41.0
50	35.97479532956422	38.0	35.0	40.0	30.0	41.0
51	34.845979393764495	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	10.0
10	11.0
11	8.0
12	14.0
13	13.0
14	19.0
15	36.0
16	47.0
17	97.0
18	202.0
19	330.0
20	618.0
21	1047.0
22	1630.0
23	2453.0
24	3256.0
25	4032.0
26	4425.0
27	5254.0
28	6272.0
29	7754.0
30	9745.0
31	12729.0
32	16879.0
33	24405.0
34	49365.0
35	72865.0
36	50339.0
37	77202.0
38	145786.0
39	189118.0
40	136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.74125896549061	21.905457343868196	26.19898178553366	11.154301905107541
2	31.40534292956014	25.25728644241462	24.911565756161977	18.425804871863264
3	29.80179360824832	25.00236844905211	27.44150659594842	17.754331346751144
4	27.528082518222487	28.094907032909056	25.66859494857186	18.708415500296603
5	22.50988554196673	30.217620386443432	26.20218830117344	21.070305770416393
6	25.182662078434287	33.84710458925264	25.01694352014202	15.95328981217106
7	75.85275097179286	7.220635968651937	10.45601024918999	6.470602810365207
8	75.20765832535348	5.395254065351704	10.52669934397605	8.870388265318764
9	68.9908075026636	8.305167008452084	13.990173487071184	8.713852001813137
10	41.05112497686208	22.647911465188173	20.387317939143248	15.913645618806505
11	32.11427438737333	23.73696077702619	23.651113608306627	20.49765122729386
12	28.55956029925536	21.11636299506051	30.594823226250284	19.729253479433844
13	22.22377689647181	29.424299267019677	29.75486187933882	18.597061957169696
14	16.44155469368302	32.497307255616136	29.694375334315694	21.36676271638515
15	14.080393177117722	26.882844121072203	40.130126234690714	18.906636467119366
16	15.875896184683642	25.142726383647933	35.421649519095524	23.559727912572896
17	15.961889104114105	25.974525690749058	27.92583620826611	30.137748996870734
18	18.47958688418503	25.987643254729974	32.24530427647161	23.28746558461339
19	21.693098558088217	27.572536485046705	27.222006025334387	23.512358931530688
20	23.358737682243046	27.166766505903635	28.098988052814228	21.375507759039095
21	20.744261430135126	29.155826457543547	28.41847361110504	21.68143850121629
22	17.21228445291742	27.79334881205883	26.654015504960626	28.340351230063128
23	17.021788273772305	29.34340762247068	26.946245680313307	26.68855842344371
24	21.667446432969978	23.6229837211031	30.283936959902526	24.425632886024403
25	16.602317727804717	28.14796029167632	27.8943540547119	27.355367925807055
26	16.68029435813573	29.82525947270308	27.86899343101546	25.625452738145732
27	19.002977687023666	29.173462293562334	29.006431978871976	22.817128040542016
28	15.38763130317168	27.646286344761645	31.551968144724622	25.41411420734205
29	16.015088113592274	24.943630912559776	31.463643213919777	27.577637759928177
30	19.317070469011213	28.297500521058794	28.1995560433346	24.185872966595394
31	20.410055050043507	30.090088514406727	23.921043924891745	25.57881251065802
32	22.414710327749624	28.235702219637577	24.771499322987946	24.578088129624852
33	19.9936452690048	28.429987917266065	26.22740317415898	25.348963639570155
34	17.387622558129028	26.479551904014414	27.23833010495509	28.894495432901472
35	18.693694678495795	24.404353282233135	27.76973719689318	29.132214842377895
36	20.893364407384894	25.818135177954332	27.007023726758227	26.281476687902543
37	18.717889296505046	27.002651205431256	30.045926049004308	24.233533449059397
38	19.305556162850184	29.12755081962912	26.047546796909504	25.51934622061119
39	19.545899085122787	25.56831845947329	28.432028427218654	26.453754028185273
40	23.622400718259502	22.367924349551018	28.015764396890848	25.993910535298635
41	19.476084494602123	22.52620962158743	25.71348616752878	32.28421971628166
42	21.23514982444327	22.56148129362501	27.654011132439297	28.549357749492422
43	22.34518723865076	22.286303951447522	29.486826321995384	25.881682487906332
44	19.182105310718654	25.42854352772106	27.922192440493625	27.467158721066664
45	18.954151198872474	24.56599082062023	27.489167078412425	28.990690902094872
46	23.158767706889492	24.194180757116644	28.040687768454593	24.60636376753928
47	17.932001463337137	22.773694328694088	31.382605818659883	27.911698389308892
48	19.79906806995451	20.665264544827817	30.765497308713126	28.77017007650455
49	20.236757454784485	19.39431834578773	32.99344267551665	27.375481523911134
50	18.92733306806704	21.094791889847443	31.44731913429908	28.530555907786443
51	18.233413933476463	21.28179005193098	26.909079249034036	33.575716765558525
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1125.0
1	1125.5
2	1126.0
3	1329.5
4	1533.0
5	1257.5
6	982.0
7	1061.5
8	1141.0
9	1284.0
10	1427.0
11	1500.0
12	1573.0
13	1661.5
14	1750.0
15	1850.5
16	1951.0
17	2051.5
18	2152.0
19	2166.0
20	2180.0
21	2087.0
22	1994.0
23	2088.5
24	2183.0
25	2574.0
26	3013.0
27	3061.0
28	3689.5
29	4318.0
30	5141.0
31	5964.0
32	7684.5
33	9405.0
34	10141.0
35	10877.0
36	13496.5
37	16116.0
38	17639.5
39	19163.0
40	24099.5
41	29036.0
42	35941.0
43	42846.0
44	51002.0
45	59158.0
46	68833.0
47	78508.0
48	90478.5
49	102449.0
50	97006.0
51	91563.0
52	74567.0
53	57571.0
54	46588.0
55	35605.0
56	29553.5
57	23502.0
58	20927.5
59	18353.0
60	17423.0
61	16493.0
62	15393.0
63	14293.0
64	11138.0
65	7983.0
66	6535.0
67	5087.0
68	4359.5
69	3632.0
70	3139.5
71	2647.0
72	2344.0
73	2041.0
74	1589.0
75	844.0
76	551.0
77	400.5
78	250.0
79	296.0
80	342.0
81	191.5
82	41.0
83	24.0
84	7.0
85	4.5
86	2.0
87	3.0
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	7.0
96	14.0
97	7.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	686103.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.34338259806495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.53733666413625	15.29863124209122
2	12.479297501878875	5.8261803228286935
3	5.935181539268493	4.156416403803495
4	3.5465656334100197	3.311553539593546
5	2.336434162521493	2.7270138285464336
6	1.6077986431269362	2.2518875320297056
7	1.1409207542348168	1.8643064776123313
8	0.866252675007079	1.6177014095429967
9	0.6580475029983506	1.382494916717261
>10	4.1639140322967565	19.467678092839943
>50	1.1780090866174349	20.087893553681738
>100	0.5357451704787692	17.36803652316588
>500	0.011345191845432761	1.993767316916607
>1k	0.0025211537434295024	1.7720414408489125
>5k	6.302884358573756E-4	0.874397399781226
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	5943	0.8661964748733061	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	4642	0.6765747999935869	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT	3448	0.5025484511800706	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	2260	0.3293966066319488	TruSeq Adapter, Index 13 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	1694	0.24690170426306257	Illumina Single End Adapter 2 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	983	0.14327294881380784	No Hit
AAAACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC	972	0.14166969099391782	No Hit
CGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG	923	0.1345279061598623	No Hit
AGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG	922	0.1343821554489632	No Hit
ACCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG	912	0.13292464833997228	No Hit
GGCCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCT	905	0.13190439336367862	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCC	874	0.12738612132580676	No Hit
GGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG	871	0.1269488691931095	TruSeq Adapter, Index 13 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG	820	0.11951558293725578	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC	744	0.10843852890892475	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	713	0.10392025687105289	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.457507108990924E-4	0.0	0.0	0.5299495848291	0.0
2	1.457507108990924E-4	0.0	0.0	2.758623705187122	0.0
3	1.457507108990924E-4	0.0	0.0	3.7833969535186407	0.0
4	1.457507108990924E-4	0.0	0.0	5.0600274302837915	0.0
5	1.457507108990924E-4	0.0	0.0	8.29919122930522	0.0
6	2.915014217981848E-4	0.0	0.0	10.126030639714445	0.0
7	2.915014217981848E-4	0.0	0.0	12.09410248898489	0.0
8	2.915014217981848E-4	0.0	0.0	15.051092911705677	0.0
9	2.915014217981848E-4	0.0	0.0	16.106619560036904	0.0
10	2.915014217981848E-4	0.0	0.0	18.346662235845056	0.0
11	2.915014217981848E-4	0.0	0.0	21.538894305956976	0.0
12	2.915014217981848E-4	0.0	0.0	23.89466304621901	0.0
13	2.915014217981848E-4	0.0	0.0	24.832714621565568	0.0
14	2.915014217981848E-4	0.0	0.0	25.181350322036195	0.0
15	2.915014217981848E-4	0.0	0.0	25.812013648096567	0.0
16	2.915014217981848E-4	0.0	0.0	27.20232967936301	0.0
17	2.915014217981848E-4	0.0	0.0	28.795676450911888	0.0
18	2.915014217981848E-4	0.0	0.0	30.75165099117771	0.0
19	2.915014217981848E-4	0.0	0.0	31.72949251059972	0.0
20	2.915014217981848E-4	0.0	0.0	32.66011079969043	0.0
21	2.915014217981848E-4	0.0	0.0	33.77831025370826	0.0
22	2.915014217981848E-4	0.0	0.0	34.92493109635142	0.0
23	2.915014217981848E-4	0.0	0.0	36.05960038070086	0.0
24	2.915014217981848E-4	0.0	0.0	36.839220933300105	0.0
25	2.915014217981848E-4	0.0	0.0	37.574387519075124	0.0
26	2.915014217981848E-4	0.0	0.0	38.236241497267905	0.0
27	4.372521326972772E-4	0.0	0.0	38.82478286787844	0.0
28	4.372521326972772E-4	0.0	0.0	39.43445809156934	0.0
29	4.372521326972772E-4	0.0	0.0	40.10214209819809	0.0
30	4.372521326972772E-4	0.0	0.0	40.88190840150823	0.0
31	4.372521326972772E-4	0.0	0.0	41.568102748421154	0.0
32	4.372521326972772E-4	0.0	0.0	42.16947018159081	0.0
33	4.372521326972772E-4	0.0	0.0	42.7813316659452	0.0
34	4.372521326972772E-4	0.0	0.0	43.38692586973093	0.0
35	4.372521326972772E-4	0.0	0.0	44.052132114274386	0.0
36	4.372521326972772E-4	0.0	0.0	44.59942603370048	0.0
37	4.372521326972772E-4	0.0	0.0	45.16901981189413	0.0
38	4.372521326972772E-4	0.0	0.0	45.727390785348554	0.0
39	4.372521326972772E-4	0.0	0.0	46.31039362894492	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCATC	35	1.210974E-7	45.000004	16
ACAACAG	25	3.889155E-5	45.000004	1
TTAGGTA	25	3.889155E-5	45.000004	18
ACTATCG	25	3.889155E-5	45.000004	19
CCTTAGA	25	3.889155E-5	45.000004	29
GTACATC	25	3.889155E-5	45.000004	44
ATTAGGA	25	3.889155E-5	45.000004	17
TGCTTAG	70	0.0	45.000004	21
ATAATCG	25	3.889155E-5	45.000004	40
CAGTTAT	25	3.889155E-5	45.000004	27
ATCAGCC	25	3.889155E-5	45.000004	39
ATTACCC	25	3.889155E-5	45.000004	16
TTCACGG	25	3.889155E-5	45.000004	2
TCGATTA	25	3.889155E-5	45.000004	41
CGACAAT	35	1.210974E-7	45.000004	20
ACGGGTA	35	1.210974E-7	45.000004	5
TCGATAC	25	3.889155E-5	45.000004	1
CCATGCG	25	3.889155E-5	45.000004	24
CACCTAA	25	3.889155E-5	45.000004	14
ATTCCGT	25	3.889155E-5	45.000004	14
>>END_MODULE