SRR2935010.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935010.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	992237
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	5545	0.5588382614234301	RNA PCR Primer, Index 33 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	5050	0.5089509865082636	Illumina Paired End PCR Primer 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT	4170	0.42026249777019	TruSeq Adapter, Index 1 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	2097	0.2113406373678869	RNA PCR Primer, Index 33 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	1774	0.17878793070607124	RNA PCR Primer, Index 33 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTG	1207	0.12164432489415332	TruSeq Adapter, Index 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCC	1175	0.11841928894004154	No Hit
GCCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTG	1106	0.11146530516398805	RNA PCR Primer, Index 33 (95% over 23bp)
AAAACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC	1009	0.10168941492808674	No Hit
ACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTG	994	0.10017767932459685	RNA PCR Primer, Index 33 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	45	3.8380676E-10	45.000004	11
AACGTAT	30	2.1652013E-6	45.000004	2
ACACGCG	30	2.1652013E-6	45.000004	26
ATCTACG	45	3.8380676E-10	45.000004	1
CGACGGA	35	1.2117016E-7	45.000004	16
ATAATCG	35	1.2117016E-7	45.000004	44
CGTGAGT	30	2.1652013E-6	45.000004	32
CTATACG	30	2.1652013E-6	45.000004	1
TTGTCGC	30	2.1652013E-6	45.000004	38
ATTGTCG	30	2.1652013E-6	45.000004	36
CGCGAGT	30	2.1652013E-6	45.000004	20
TAACGCC	30	2.1652013E-6	45.000004	12
AGTTGCA	30	2.1652013E-6	45.000004	35
TCGTCGG	30	2.1652013E-6	45.000004	10
TATTATG	30	2.1652013E-6	45.000004	37
CTCGCAC	35	1.2117016E-7	45.000004	26
TCCAACG	30	2.1652013E-6	45.000004	28
CGTAAGA	30	2.1652013E-6	45.000004	29
CGTCGAC	30	2.1652013E-6	45.000004	17
GCGGATA	35	1.2117016E-7	45.000004	1