##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935010.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	992237
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.28117173618803	33.0	31.0	34.0	30.0	34.0
2	32.4502291287263	34.0	31.0	34.0	31.0	34.0
3	32.459773219503	34.0	31.0	34.0	30.0	34.0
4	35.98699403469131	37.0	35.0	37.0	35.0	37.0
5	35.905117426582564	37.0	35.0	37.0	35.0	37.0
6	35.844913060085446	37.0	35.0	37.0	35.0	37.0
7	36.09573116100287	37.0	35.0	37.0	35.0	37.0
8	35.855025563449054	37.0	35.0	37.0	35.0	37.0
9	37.59997762631307	39.0	38.0	39.0	35.0	39.0
10	37.44995802414141	39.0	37.0	39.0	35.0	39.0
11	37.345893168668375	39.0	37.0	39.0	35.0	39.0
12	37.15538626356405	39.0	37.0	39.0	34.0	39.0
13	37.0409942382717	39.0	37.0	39.0	33.0	39.0
14	38.15085307240105	40.0	37.0	41.0	33.0	41.0
15	38.25684488685667	40.0	37.0	41.0	34.0	41.0
16	38.32428845124703	40.0	37.0	41.0	34.0	41.0
17	38.3512920804203	40.0	37.0	41.0	34.0	41.0
18	38.348346211640965	40.0	37.0	41.0	34.0	41.0
19	38.270037299556456	40.0	36.0	41.0	34.0	41.0
20	38.2320231960711	40.0	36.0	41.0	34.0	41.0
21	38.15362156420291	40.0	36.0	41.0	34.0	41.0
22	38.08968220294143	40.0	36.0	41.0	34.0	41.0
23	37.89383282421438	40.0	36.0	41.0	34.0	41.0
24	37.71236105890024	39.0	35.0	41.0	33.0	41.0
25	37.6413175481261	39.0	35.0	41.0	33.0	41.0
26	37.80952232178401	40.0	35.0	41.0	34.0	41.0
27	37.82847847842804	40.0	36.0	41.0	34.0	41.0
28	37.82994788543463	40.0	36.0	41.0	34.0	41.0
29	37.83350953451645	40.0	36.0	41.0	34.0	41.0
30	37.71084428417807	40.0	36.0	41.0	34.0	41.0
31	37.53296944177651	40.0	35.0	41.0	33.0	41.0
32	37.42743921059182	40.0	35.0	41.0	33.0	41.0
33	37.33498650020106	39.0	35.0	41.0	33.0	41.0
34	37.18868576761399	39.0	35.0	41.0	33.0	41.0
35	37.0840514917303	39.0	35.0	41.0	32.0	41.0
36	37.00167500304867	39.0	35.0	41.0	32.0	41.0
37	36.85661288583272	39.0	35.0	41.0	32.0	41.0
38	36.829151704683454	39.0	35.0	41.0	32.0	41.0
39	36.77336765309094	39.0	35.0	41.0	31.0	41.0
40	36.643691980847315	39.0	35.0	41.0	31.0	41.0
41	36.6024911387098	39.0	35.0	41.0	31.0	41.0
42	36.619457851299636	39.0	35.0	41.0	31.0	41.0
43	36.56544152253947	39.0	35.0	41.0	31.0	41.0
44	36.440127711423784	39.0	35.0	41.0	31.0	41.0
45	36.376505814639046	39.0	35.0	41.0	31.0	41.0
46	36.22567491436018	38.0	35.0	40.0	31.0	41.0
47	36.120805815546085	38.0	35.0	40.0	31.0	41.0
48	36.101505991008196	38.0	35.0	40.0	31.0	41.0
49	36.0663903885866	38.0	35.0	40.0	31.0	41.0
50	35.9666450656446	38.0	35.0	40.0	31.0	41.0
51	34.909637516036994	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	20.0
10	19.0
11	26.0
12	22.0
13	14.0
14	33.0
15	36.0
16	78.0
17	149.0
18	254.0
19	527.0
20	848.0
21	1479.0
22	2196.0
23	3340.0
24	4719.0
25	5629.0
26	6418.0
27	7216.0
28	8282.0
29	10066.0
30	12438.0
31	16237.0
32	21817.0
33	31860.0
34	64005.0
35	100996.0
36	72396.0
37	115129.0
38	211539.0
39	294162.0
40	283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.954530016518234	22.716246219401214	24.906549544110934	11.422674219969624
2	30.14350402172062	25.040287753833006	26.926833004614824	17.889375219831553
3	30.69730316446575	25.536237814151256	26.951020774270663	16.815438247112333
4	29.08538988165126	26.059197550585196	26.238187046038398	18.617225521725153
5	23.977739189326744	30.58462847081897	24.575681011693778	20.86195132816051
6	26.01475252384259	32.91874824260736	25.37468366932497	15.69181556422508
7	77.81114794146963	7.367393072421207	8.745995160430422	6.075463825678744
8	78.47389283003959	5.170034981561865	8.969631247373359	7.386440941025178
9	72.86132244614946	7.427660931813669	11.904514747988635	7.806501874048237
10	42.714996517969	21.76687626040956	18.36406019932738	17.15406702229407
11	29.772927234118463	24.649252144396954	24.234734241920027	21.34308637956456
12	26.15050638103598	21.45223369013653	31.735059265074778	20.66220066375271
13	22.35191793896015	27.478918847009332	32.00545837335233	18.163704840678186
14	16.661745127424194	30.608614675727676	30.92204785751791	21.80759233933022
15	14.595101775079947	25.74808236338697	39.25926971076466	20.397546150768413
16	16.82702822007242	24.219919233005825	34.761755508008676	24.19129703891308
17	17.456011013497783	24.676463385259773	28.994282615947604	28.873242985294844
18	18.820503569207762	25.153063229853352	31.796838860070732	24.22959434086816
19	20.929979430317555	27.458460025175434	28.081698223307537	23.52986232119947
20	24.30125060847358	25.94712755117981	28.071116074083108	21.6805057662635
21	21.155127252863984	28.425567681914703	28.70362625058328	21.715678814638036
22	19.482341416415636	25.37649775204916	26.86364245638895	28.27751837514626
23	18.443577492070947	26.905064011924573	27.70497371091786	26.94638478508663
24	21.154824905743286	23.767003246200254	30.347285981071053	24.730885866985407
25	18.65370874095604	27.758388368907834	27.800918530552682	25.78698435958345
26	18.461214407444995	29.131346643997354	28.52745866158992	23.87998028696773
27	20.3347587320368	29.2435174257763	27.64541132814035	22.77631251404654
28	18.74602539514249	25.604669045802563	30.681782678936585	24.96752288011836
29	18.50807821115318	24.864825641454612	30.861276086257618	25.765820061134587
30	22.638240662261133	27.381663856518152	26.719422879816012	23.260672601404707
31	23.618752374684675	29.096173595622822	23.736566969383322	23.54850706030918
32	22.52919413406273	28.743636852888976	23.401264012529268	25.32590500051903
33	23.978747013062403	27.44273797489914	23.45246145830079	25.126053553737666
34	21.751557339627528	26.59445273659418	26.237582351797002	25.416407571981292
35	20.43261841676938	27.291463632176587	25.375590710687064	26.900327240366966
36	24.134858909716126	27.764233746574657	25.19267070266479	22.90823664104443
37	21.642712376176256	29.27506230870246	26.647061135595628	22.435164179525657
38	21.941330549052292	28.614635414724503	23.442584785691324	26.001449250531877
39	23.381208320189632	24.51873897062899	26.155646282087847	25.944406427093526
40	22.683693512739396	26.14959933967389	27.761311057741246	23.40539608984547
41	18.572276583114718	28.24335315050739	25.86005158041879	27.3243186859591
42	20.910024520351488	26.147079780334735	27.15359334513831	25.789302354175465
43	23.5410491646653	24.069753496392497	27.27543923477959	25.113758104162613
44	22.230979090680954	24.07569965643289	27.108342059407175	26.584979193478976
45	21.817065882445423	24.978911288331314	25.711901491276784	27.49212133794648
46	24.20066979965472	25.832537992435274	26.799645649174543	23.167146558735464
47	19.9960291744815	26.180539528358647	29.484891210466852	24.338540086692998
48	20.457108533545917	23.90114458541659	28.68447759960574	26.957269281431756
49	21.777156062513292	21.213177900037994	31.463148421193726	25.546517616254988
50	20.35894650169264	22.378927615075835	30.347588328191755	26.914537555039775
51	19.377729312654136	22.956108268488272	27.367957453713178	30.298204965144414
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1269.0
1	1409.5
2	1550.0
3	1961.5
4	2373.0
5	1897.5
6	1422.0
7	1608.0
8	1794.0
9	2066.5
10	2339.0
11	2442.0
12	2545.0
13	2599.5
14	2654.0
15	2622.0
16	2590.0
17	2685.0
18	2780.0
19	2856.0
20	2932.0
21	3103.0
22	3274.0
23	2959.0
24	2644.0
25	3240.0
26	4558.5
27	5281.0
28	6534.5
29	7788.0
30	10265.5
31	12743.0
32	13068.0
33	13393.0
34	16975.0
35	20557.0
36	22844.5
37	25132.0
38	27085.0
39	29038.0
40	34161.5
41	39285.0
42	50555.0
43	61825.0
44	69116.0
45	76407.0
46	88954.5
47	101502.0
48	116723.0
49	131944.0
50	126813.0
51	121682.0
52	99368.0
53	77054.0
54	64163.5
55	51273.0
56	46169.0
57	41065.0
58	36709.0
59	32353.0
60	31279.0
61	30205.0
62	27126.5
63	24048.0
64	20300.5
65	16553.0
66	14042.0
67	11531.0
68	10899.5
69	10268.0
70	8428.5
71	6589.0
72	5487.5
73	4386.0
74	3528.5
75	2026.0
76	1381.0
77	1380.0
78	1379.0
79	994.5
80	610.0
81	373.5
82	137.0
83	126.0
84	115.0
85	67.5
86	20.0
87	17.0
88	14.0
89	9.0
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	992237.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.463442915338888
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.13466915858291	14.194776962100677
2	12.363685936933514	5.307345346611013
3	5.85420782688671	3.7695436652073906
4	3.515442681969036	3.01814013306353
5	2.2389932629076608	2.4028252043123466
6	1.5536798257448259	2.000839094915264
7	1.0792753299998963	1.621547510477042
8	0.8358082149310088	1.4351457527474403
9	0.6362433269967567	1.2290375096334163
>10	3.748365025876199	15.755881644970712
>50	1.0738634616276401	17.070567283996027
>100	0.9534390380514586	28.662743487497494
>500	0.008059902360454896	1.177575257527731
>1k	0.0033187833248931923	1.2758761302179997
>5k	9.482238071123406E-4	1.0781550167219418
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	5545	0.5588382614234301	RNA PCR Primer, Index 33 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	5050	0.5089509865082636	Illumina Paired End PCR Primer 2 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGCT	4170	0.42026249777019	TruSeq Adapter, Index 1 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	2097	0.2113406373678869	RNA PCR Primer, Index 33 (95% over 24bp)
ACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTGC	1774	0.17878793070607124	RNA PCR Primer, Index 33 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTG	1207	0.12164432489415332	TruSeq Adapter, Index 1 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCC	1175	0.11841928894004154	No Hit
GCCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTG	1106	0.11146530516398805	RNA PCR Primer, Index 33 (95% over 23bp)
AAAACTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTC	1009	0.10168941492808674	No Hit
ACCTGTCTCTTATACACATCTGACGCTAGCGAACTCGTATGCCGTCTTCTG	994	0.10017767932459685	RNA PCR Primer, Index 33 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.0078237356599281E-4	0.0	0.0	0.44606278540308414	0.0
2	1.0078237356599281E-4	0.0	0.0	1.9954909966066574	0.0
3	1.0078237356599281E-4	0.0	0.0	2.7918733125251327	0.0
4	1.0078237356599281E-4	0.0	0.0	3.712721859797609	0.0
5	1.0078237356599281E-4	0.0	0.0	6.161028060836272	0.0
6	1.0078237356599281E-4	0.0	0.0	7.551220121805577	0.0
7	1.0078237356599281E-4	0.0	0.0	9.016998962949376	0.0
8	1.0078237356599281E-4	0.0	0.0	11.34255223298466	0.0
9	1.0078237356599281E-4	0.0	0.0	12.190938253663187	0.0
10	1.0078237356599281E-4	0.0	0.0	13.979623819712428	0.0
11	1.0078237356599281E-4	0.0	0.0	16.52296779902382	0.0
12	1.0078237356599281E-4	0.0	0.0	18.432592213352255	0.0
13	1.0078237356599281E-4	0.0	0.0	19.21940020378196	0.0
14	1.0078237356599281E-4	0.0	0.0	19.4877836645882	0.0
15	1.0078237356599281E-4	0.0	0.0	20.006409758958796	0.0
16	1.0078237356599281E-4	0.0	0.0	21.131947306943804	0.0
17	1.0078237356599281E-4	0.0	0.0	22.48918353175703	0.0
18	1.0078237356599281E-4	0.0	0.0	24.221330186235747	0.0
19	1.0078237356599281E-4	0.0	0.0	25.08392652158708	0.0
20	1.0078237356599281E-4	0.0	0.0	25.84574048337242	0.0
21	1.0078237356599281E-4	0.0	0.0	26.79722687220896	0.0
22	1.0078237356599281E-4	0.0	0.0	27.739743629798124	0.0
23	1.0078237356599281E-4	0.0	0.0	28.725092896152834	0.0
24	1.0078237356599281E-4	0.0	0.0	29.454152586529226	0.0
25	1.0078237356599281E-4	0.0	0.0	30.11730060459346	0.0
26	1.0078237356599281E-4	0.0	0.0	30.680774855200927	0.0
27	1.0078237356599281E-4	0.0	0.0	31.23971389899792	0.0
28	2.0156474713198562E-4	0.0	0.0	31.79704042481786	0.0
29	2.0156474713198562E-4	0.0	0.0	32.428139648088106	0.0
30	2.0156474713198562E-4	0.0	0.0	33.144500759395186	0.0
31	2.0156474713198562E-4	0.0	0.0	33.76894834601008	0.0
32	2.0156474713198562E-4	0.0	0.0	34.294629206530296	0.0
33	2.0156474713198562E-4	0.0	0.0	34.839458718028055	0.0
34	2.0156474713198562E-4	0.0	0.0	35.379047546100374	0.0
35	2.0156474713198562E-4	0.0	0.0	35.981625357651446	0.0
36	2.0156474713198562E-4	0.0	0.0	36.52786582237913	0.0
37	2.0156474713198562E-4	0.0	0.0	37.06201240227889	0.0
38	2.0156474713198562E-4	0.0	0.0	37.54546544827496	0.0
39	2.0156474713198562E-4	0.0	0.0	38.03768656077127	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	45	3.8380676E-10	45.000004	11
AACGTAT	30	2.1652013E-6	45.000004	2
ACACGCG	30	2.1652013E-6	45.000004	26
ATCTACG	45	3.8380676E-10	45.000004	1
CGACGGA	35	1.2117016E-7	45.000004	16
ATAATCG	35	1.2117016E-7	45.000004	44
CGTGAGT	30	2.1652013E-6	45.000004	32
CTATACG	30	2.1652013E-6	45.000004	1
TTGTCGC	30	2.1652013E-6	45.000004	38
ATTGTCG	30	2.1652013E-6	45.000004	36
CGCGAGT	30	2.1652013E-6	45.000004	20
TAACGCC	30	2.1652013E-6	45.000004	12
AGTTGCA	30	2.1652013E-6	45.000004	35
TCGTCGG	30	2.1652013E-6	45.000004	10
TATTATG	30	2.1652013E-6	45.000004	37
CTCGCAC	35	1.2117016E-7	45.000004	26
TCCAACG	30	2.1652013E-6	45.000004	28
CGTAAGA	30	2.1652013E-6	45.000004	29
CGTCGAC	30	2.1652013E-6	45.000004	17
GCGGATA	35	1.2117016E-7	45.000004	1
>>END_MODULE