##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935008.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	696114
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18512628678636	33.0	31.0	34.0	30.0	34.0
2	32.370684974012875	34.0	31.0	34.0	30.0	34.0
3	32.36502354499407	34.0	31.0	34.0	30.0	34.0
4	35.90860261393967	37.0	35.0	37.0	35.0	37.0
5	35.83293397345837	37.0	35.0	37.0	35.0	37.0
6	35.77595049086788	37.0	35.0	37.0	35.0	37.0
7	36.052856285033776	37.0	35.0	37.0	35.0	37.0
8	35.77838112722916	37.0	35.0	37.0	35.0	37.0
9	37.50963347957375	39.0	37.0	39.0	35.0	39.0
10	37.41982204064277	39.0	37.0	39.0	34.0	39.0
11	37.342241357019105	39.0	37.0	39.0	35.0	39.0
12	37.03680144344173	39.0	35.0	39.0	34.0	39.0
13	36.81395432357344	39.0	35.0	39.0	33.0	39.0
14	37.82550702902111	40.0	36.0	41.0	33.0	41.0
15	37.96818911844899	40.0	36.0	41.0	33.0	41.0
16	38.05942561132228	40.0	36.0	41.0	34.0	41.0
17	38.095991461168715	40.0	36.0	41.0	34.0	41.0
18	38.11209945497433	40.0	36.0	41.0	34.0	41.0
19	38.058481800394766	40.0	36.0	41.0	34.0	41.0
20	38.01172796409784	40.0	36.0	41.0	34.0	41.0
21	37.92933915996518	40.0	35.0	41.0	34.0	41.0
22	37.855729090350145	39.0	35.0	41.0	34.0	41.0
23	37.63662561017305	39.0	35.0	41.0	33.0	41.0
24	37.42308012768024	39.0	35.0	41.0	33.0	41.0
25	37.380634493775446	39.0	35.0	41.0	33.0	41.0
26	37.56683531720379	39.0	35.0	41.0	33.0	41.0
27	37.58729604633724	39.0	35.0	41.0	33.0	41.0
28	37.63637996075355	39.0	35.0	41.0	33.0	41.0
29	37.705611437207125	39.0	36.0	41.0	34.0	41.0
30	37.61360782860279	39.0	36.0	41.0	34.0	41.0
31	37.525872199093826	39.0	35.0	41.0	33.0	41.0
32	37.41258184722617	39.0	35.0	41.0	33.0	41.0
33	37.2693294489121	39.0	35.0	41.0	33.0	41.0
34	37.22309851547304	39.0	35.0	41.0	33.0	41.0
35	37.168156365193056	39.0	35.0	41.0	33.0	41.0
36	37.04922182286235	39.0	35.0	41.0	33.0	41.0
37	36.99290058812206	39.0	35.0	41.0	33.0	41.0
38	36.91080914907616	39.0	35.0	41.0	32.0	41.0
39	36.88376329164476	39.0	35.0	41.0	32.0	41.0
40	36.829789373579615	39.0	35.0	41.0	32.0	41.0
41	36.8079423772543	39.0	35.0	41.0	32.0	41.0
42	36.783995150219646	39.0	35.0	41.0	32.0	41.0
43	36.670558270628085	39.0	35.0	41.0	32.0	41.0
44	36.529999396650545	39.0	35.0	41.0	31.0	41.0
45	36.565214892962935	39.0	35.0	41.0	31.0	41.0
46	36.454909684333316	39.0	35.0	41.0	31.0	41.0
47	36.400056599924724	39.0	35.0	40.0	31.0	41.0
48	36.37675581873084	39.0	35.0	40.0	31.0	41.0
49	36.3384632402164	39.0	35.0	40.0	31.0	41.0
50	36.26609147352301	39.0	35.0	40.0	31.0	41.0
51	35.30018071752615	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	8.0
10	13.0
11	9.0
12	7.0
13	10.0
14	21.0
15	25.0
16	48.0
17	89.0
18	193.0
19	392.0
20	683.0
21	1132.0
22	1740.0
23	2644.0
24	3443.0
25	4005.0
26	4567.0
27	5030.0
28	5642.0
29	6715.0
30	8618.0
31	11247.0
32	15160.0
33	22736.0
34	48633.0
35	77681.0
36	47117.0
37	73279.0
38	143070.0
39	212021.0
40	133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.40577836388867	23.85270228726904	25.893890943150115	11.84762840569217
2	31.083270843568727	24.163714563993828	26.39495829706054	18.358056295376908
3	29.917800819980634	25.619079633508306	27.60883993139055	16.85427961512051
4	28.733224730432084	26.343386284430427	26.1023338131398	18.82105517199769
5	23.59627877043128	30.667103376745764	25.550700029018238	20.185917823804722
6	25.258075545097498	31.885438304645504	25.853093027866127	17.00339312239087
7	74.27964385143812	7.486992073137445	11.528284160353046	6.705079915071383
8	73.09320025168292	5.865993213755218	11.498547651677743	9.542258882884125
9	67.59855425979077	8.414282718060548	14.343483969579696	9.643679052568977
10	39.21311739169159	26.415644563965095	20.661558307978293	13.709679736365022
11	32.76244982862003	23.040191692739985	25.16110866898238	19.036249809657612
12	29.05414917671531	21.63553670806793	30.965617700549046	18.344696414667713
13	22.46815895097642	29.897976480863765	29.453221742415753	18.18064282574406
14	15.832751532076642	34.07545315853438	28.326107505379866	21.76568780400911
15	12.914695006852325	27.713564157594877	40.42470055192109	18.947040283631704
16	14.241632836001001	27.783523963029044	35.07026148015986	22.904581720810096
17	15.322346627132912	26.661293983456737	27.161930373473307	30.854429015937047
18	17.532042165507374	26.898898743596593	32.833558871104444	22.735500219791586
19	20.465469736278827	27.455273130550456	27.787115328811083	24.29214180435963
20	22.80416713354422	28.220234042125288	28.38529321346791	20.59030561086259
21	19.7921604794617	29.94006728782929	30.274064305559143	19.993707927149863
22	16.781590371691994	28.36546887435104	26.046595816202515	28.806344937754446
23	17.000232720502677	28.557535116374616	28.471773301499464	25.970458861623243
24	21.144381523715943	24.802115745409516	29.498185642006913	24.55531708886763
25	16.439548694610366	28.727191235918255	28.152429056160344	26.68083101331104
26	15.633502558488981	31.313261908250663	30.06016255958076	22.9930729736796
27	18.83125465081869	32.96054956515743	28.408277954472975	19.799917829550907
28	15.494732184670902	26.939840313511866	33.88985137491848	23.675576126898758
29	18.675963994403215	23.808743970096852	33.65382681572271	23.86146521977722
30	19.693469747771196	28.711676535739837	27.717299178008197	23.877554538480766
31	20.577951312572367	27.79401075111261	29.44862479421474	22.179413142100287
32	24.001241176014272	26.867725688608473	25.190845177657685	23.94018795771957
33	21.258012337059736	29.498185642006913	24.02278937070652	25.221012650226832
34	17.383072312868293	24.40275587044651	33.15132291549947	25.062848901185724
35	18.417816621990077	24.460792341484296	28.457264183740023	28.66412685278561
36	20.484001183714163	28.016819084230455	29.700738672114053	21.798441059941332
37	19.365362569923892	27.187788207104006	33.683706979029296	19.763142243942802
38	19.560732868466946	27.467483774209395	26.339938573279664	26.631844784043995
39	21.860068896761163	24.93499627934505	29.538121629503213	23.666813194390574
40	21.11205923167757	21.463294805161222	32.33249151719402	25.092154445967186
41	15.849271814674031	25.134819871457836	28.722450633085963	30.293457680782172
42	20.022582508037477	24.05798475537053	29.804600970530686	26.114831766061307
43	23.182409777708823	24.36253257368764	29.017517245738482	23.437540402865046
44	19.636151549889817	24.1889977790994	29.78736241477689	26.387488256233894
45	20.438175356335314	23.169337206262192	26.808252671257872	29.584234766144625
46	21.94223934585427	23.33037404792893	29.661089993880314	25.066296612336487
47	17.02565959023953	23.052833300292768	34.198277868280194	25.723229241187507
48	18.574974788612213	20.82704844321476	31.923794091197706	28.674182676975324
49	20.52551737215456	19.504276598373256	35.72963623774267	24.240569791729516
50	19.40932088709608	19.68844183567634	32.57095820512158	28.331279072106007
51	17.249473505776354	20.723473454060684	28.01279675455457	34.01425628560839
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1633.0
1	1529.5
2	1426.0
3	1821.0
4	2216.0
5	1817.0
6	1418.0
7	1508.5
8	1599.0
9	1867.5
10	2136.0
11	2131.5
12	2127.0
13	2206.5
14	2286.0
15	2390.5
16	2495.0
17	2416.0
18	2337.0
19	2470.5
20	2604.0
21	2327.5
22	2051.0
23	2352.0
24	2653.0
25	2884.5
26	3417.0
27	3718.0
28	4714.0
29	5710.0
30	6558.0
31	7406.0
32	8497.0
33	9588.0
34	11419.5
35	13251.0
36	14213.0
37	15175.0
38	18272.0
39	21369.0
40	26981.0
41	32593.0
42	43228.0
43	53863.0
44	68755.0
45	83647.0
46	96570.0
47	109493.0
48	104212.0
49	98931.0
50	78873.5
51	58816.0
52	48080.0
53	37344.0
54	32635.5
55	27927.0
56	24218.5
57	20510.0
58	18302.5
59	16095.0
60	15475.5
61	14856.0
62	13368.0
63	11880.0
64	11060.5
65	10241.0
66	8035.0
67	5829.0
68	4508.0
69	3187.0
70	2636.0
71	2085.0
72	1609.0
73	1133.0
74	837.0
75	404.0
76	267.0
77	298.5
78	330.0
79	185.0
80	40.0
81	63.5
82	87.0
83	91.0
84	95.0
85	48.5
86	2.0
87	2.5
88	3.0
89	3.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	696114.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.653065623016595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.492985240326426	13.748177760100122
2	12.699873282735693	5.499823791901423
3	6.319316163620117	4.1049770275036765
4	3.81887042529332	3.307610076986942
5	2.6124640049255876	2.828392726821125
6	1.7794122462933457	2.311783808363351
7	1.3319766434731195	2.0188964368614184
8	1.0072545868584597	1.744811973466456
9	0.7373182662856022	1.436868072443787
>10	4.458154586423197	18.660721685605044
>50	1.0399337747875856	16.40330676406661
>100	0.684360797059184	21.477703712904578
>500	0.010044434399107396	1.5041801853657475
>1k	0.006026660639464438	2.065601592512092
>5k	0.0020088868798214792	2.887144385097625
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	7910	1.136308133437914	No Hit
GCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	6802	0.9771388019778369	No Hit
CTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGCT	5200	0.747004082664621	No Hit
TCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	2944	0.4229192344932008	No Hit
ACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTGC	2443	0.3509482642210902	No Hit
CGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG	1553	0.22309564238041468	No Hit
ACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG	1413	0.2029839940009826	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1399	0.2009728291630394	No Hit
AAAACTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTC	1332	0.19134796886716832	No Hit
GCCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG	1087	0.15615258420316214	No Hit
AGACATAGACCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCC	1047	0.1504063989518958	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCC	1028	0.1476769609575443	No Hit
AGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG	981	0.1409251932873064	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTC	959	0.13776479139910994	No Hit
GGCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCTG	923	0.13259322467297024	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	768	0.11032675682431327	No Hit
GGCCTGTCTCTTATACACATCTGACGCTATGCTCATCGTATGCCGTCTTCT	701	0.10070189652844218	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7682649680943064	0.0
2	0.0	0.0	0.0	3.757861499696889	0.0
3	0.0	0.0	0.0	5.098877482711165	0.0
4	1.436546312816579E-4	0.0	0.0	6.507267487796539	0.0
5	1.436546312816579E-4	0.0	0.0	10.231801113036083	0.0
6	1.436546312816579E-4	0.0	0.0	11.97303889880106	0.0
7	1.436546312816579E-4	0.0	0.0	13.897579994081429	0.0
8	1.436546312816579E-4	0.0	0.0	16.694248355872745	0.0
9	1.436546312816579E-4	0.0	0.0	17.591802492120543	0.0
10	1.436546312816579E-4	0.0	0.0	19.65540127048156	0.0
11	1.436546312816579E-4	0.0	0.0	23.059585067963006	0.0
12	2.873092625633158E-4	0.0	0.0	25.62640601970367	0.0
13	2.873092625633158E-4	0.0	0.0	26.574670240793893	0.0
14	2.873092625633158E-4	0.0	0.0	26.95320019422106	0.0
15	2.873092625633158E-4	0.0	0.0	27.642455115110454	0.0
16	2.873092625633158E-4	0.0	0.0	29.073686206569615	0.0
17	2.873092625633158E-4	0.0	0.0	30.75789310371577	0.0
18	2.873092625633158E-4	0.0	0.0	32.83643196373008	0.0
19	2.873092625633158E-4	0.0	0.0	33.876922458103124	0.0
20	2.873092625633158E-4	0.0	0.0	34.90922463849312	0.0
21	2.873092625633158E-4	0.0	0.0	36.06952309535507	0.0
22	2.873092625633158E-4	0.0	0.0	37.20382006395504	0.0
23	2.873092625633158E-4	0.0	0.0	38.27289782995314	0.0
24	2.873092625633158E-4	0.0	0.0	39.04575974624846	0.0
25	2.873092625633158E-4	0.0	0.0	39.76647503138854	0.0
26	2.873092625633158E-4	0.0	0.0	40.405881795223195	0.0
27	2.873092625633158E-4	0.0	0.0	41.05103474430912	0.0
28	7.182731564082895E-4	0.0	0.0	41.697767894339144	0.0
29	7.182731564082895E-4	0.0	0.0	42.350821848145564	0.0
30	7.182731564082895E-4	0.0	0.0	43.15284565459106	0.0
31	8.619277876899473E-4	0.0	0.0	43.88692082044033	0.0
32	8.619277876899473E-4	0.0	0.0	44.492856055186365	0.0
33	8.619277876899473E-4	0.0	0.0	45.05468931812893	0.0
34	8.619277876899473E-4	0.0	0.0	45.637783466501176	0.0
35	8.619277876899473E-4	0.0	0.0	46.30204248154756	0.0
36	8.619277876899473E-4	0.0	0.0	46.889877232752106	0.0
37	8.619277876899473E-4	0.0	0.0	47.50960331210118	0.0
38	8.619277876899473E-4	0.0	0.0	48.01957725315106	0.0
39	8.619277876899473E-4	0.0	0.0	48.58658208281977	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	35	1.2110104E-7	45.000004	24
CGGCCTA	35	1.2110104E-7	45.000004	19
AACCACG	45	3.8380676E-10	45.000004	1
CGCGCCA	35	1.2110104E-7	45.000004	15
CAATTGC	45	3.8380676E-10	45.000004	24
TACGGGT	35	1.2110104E-7	45.000004	4
CATTCGA	35	1.2110104E-7	45.000004	10
TACATTC	35	1.2110104E-7	45.000004	14
CTTGGGT	35	1.2110104E-7	45.000004	4
CTGCGGC	35	1.2110104E-7	45.000004	21
ACGATTC	35	1.2110104E-7	45.000004	42
AGACGAC	35	1.2110104E-7	45.000004	10
GCATTCG	35	1.2110104E-7	45.000004	9
GTTCGGA	35	1.2110104E-7	45.000004	29
TTCTTCG	45	3.8380676E-10	45.000004	1
TACGATT	35	1.2110104E-7	45.000004	41
CTTGCGC	35	1.2110104E-7	45.000004	11
GCTTAGC	35	1.2110104E-7	45.000004	12
GGTATCG	35	1.2110104E-7	45.000004	8
GGTGCGA	35	1.2110104E-7	45.000004	8
>>END_MODULE