SRR2935006.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2935006.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1651458
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	8307	0.5030100674676559	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	7102	0.43004423969607464	TruSeq Adapter, Index 22 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	5160	0.3124511795032026	Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	3093	0.1872890500394197	TruSeq Adapter, Index 22 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	2364	0.14314623805146723	No Hit
GCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG	1658	0.10039613480936238	TruSeq Adapter, Index 22 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGC	20	7.0341816E-4	45.000004	20
CGTTGTC	20	7.0341816E-4	45.000004	22
CGGCATA	60	0.0	45.000004	23
CGTTCGC	20	7.0341816E-4	45.000004	28
TAACACG	20	7.0341816E-4	45.000004	1
GTCGTTA	40	6.8175723E-9	45.000004	31
TTACGCG	20	7.0341816E-4	45.000004	1
CGTCGAA	20	7.0341816E-4	45.000004	42
CACGTAA	20	7.0341816E-4	45.000004	29
GCTCGTC	30	2.1660799E-6	45.000004	9
ATCGCCT	20	7.0341816E-4	45.000004	31
ATCGCCC	20	7.0341816E-4	45.000004	16
TAGTCGT	20	7.0341816E-4	45.000004	15
CCGCAAT	20	7.0341816E-4	45.000004	26
CTTACGA	20	7.0341816E-4	45.000004	29
TATCCCG	20	7.0341816E-4	45.000004	10
CTGTCGT	35	1.2123564E-7	45.0	37
GATCGCG	25	3.8915496E-5	45.0	1
CCTACGG	25	3.8915496E-5	45.0	2
TTACTCG	25	3.8915496E-5	45.0	35