##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935006.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1651458
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.27764436031676	33.0	31.0	34.0	30.0	34.0
2	32.44111566870002	34.0	31.0	34.0	31.0	34.0
3	32.443693390930925	34.0	31.0	34.0	30.0	34.0
4	35.97420218982257	37.0	35.0	37.0	35.0	37.0
5	35.89829048029075	37.0	35.0	37.0	35.0	37.0
6	35.83059877998714	37.0	35.0	37.0	35.0	37.0
7	36.115157030938725	37.0	35.0	37.0	35.0	37.0
8	35.84657920455743	37.0	35.0	37.0	35.0	37.0
9	37.584090543023194	39.0	38.0	39.0	35.0	39.0
10	37.37712978471145	39.0	37.0	39.0	34.0	39.0
11	37.33749995458558	39.0	37.0	39.0	34.0	39.0
12	37.179158053065834	39.0	37.0	39.0	34.0	39.0
13	37.121145678545865	39.0	37.0	39.0	33.0	39.0
14	38.244113383446624	40.0	37.0	41.0	33.0	41.0
15	38.31036817163985	40.0	37.0	41.0	34.0	41.0
16	38.366305410128504	40.0	37.0	41.0	34.0	41.0
17	38.388147321942185	40.0	37.0	41.0	34.0	41.0
18	38.39467185965371	40.0	37.0	41.0	34.0	41.0
19	38.35089236299076	40.0	37.0	41.0	34.0	41.0
20	38.33393643677284	40.0	37.0	41.0	34.0	41.0
21	38.2788233185464	40.0	37.0	41.0	34.0	41.0
22	38.22497695975314	40.0	37.0	41.0	34.0	41.0
23	38.003541718893246	40.0	36.0	41.0	34.0	41.0
24	37.79399415546747	40.0	36.0	41.0	33.0	41.0
25	37.762193165069895	40.0	36.0	41.0	33.0	41.0
26	37.93046507994754	40.0	36.0	41.0	34.0	41.0
27	37.977739670037025	40.0	36.0	41.0	34.0	41.0
28	37.94261010573687	40.0	36.0	41.0	34.0	41.0
29	37.952200419265886	40.0	36.0	41.0	34.0	41.0
30	37.87080264832651	40.0	36.0	41.0	34.0	41.0
31	37.73900819760478	40.0	36.0	41.0	33.0	41.0
32	37.625134275288865	40.0	36.0	41.0	33.0	41.0
33	37.5665218249571	40.0	36.0	41.0	33.0	41.0
34	37.4466295842825	40.0	36.0	41.0	33.0	41.0
35	37.35923226627622	40.0	35.0	41.0	33.0	41.0
36	37.34749597022752	40.0	35.0	41.0	33.0	41.0
37	37.26196609299177	39.0	35.0	41.0	33.0	41.0
38	37.192155658817846	39.0	35.0	41.0	32.0	41.0
39	37.12246148554792	39.0	35.0	41.0	32.0	41.0
40	37.08173504866609	39.0	35.0	41.0	32.0	41.0
41	37.04582011773839	39.0	35.0	41.0	32.0	41.0
42	37.07692596481412	39.0	35.0	41.0	32.0	41.0
43	37.003872335839	39.0	35.0	41.0	32.0	41.0
44	36.86898728275257	39.0	35.0	41.0	32.0	41.0
45	36.82625958395551	39.0	35.0	41.0	31.0	41.0
46	36.72429816562092	39.0	35.0	41.0	31.0	41.0
47	36.652716569237604	39.0	35.0	41.0	31.0	41.0
48	36.60606748703267	39.0	35.0	41.0	31.0	41.0
49	36.546801069115894	39.0	35.0	41.0	31.0	41.0
50	36.45010469536616	39.0	35.0	40.0	31.0	41.0
51	35.3849216873817	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	11.0
9	16.0
10	28.0
11	15.0
12	21.0
13	21.0
14	28.0
15	47.0
16	74.0
17	176.0
18	370.0
19	649.0
20	1101.0
21	1770.0
22	2685.0
23	3992.0
24	5498.0
25	7489.0
26	9109.0
27	11056.0
28	13440.0
29	16606.0
30	20946.0
31	27403.0
32	37071.0
33	53024.0
34	100555.0
35	137449.0
36	118520.0
37	190769.0
38	357794.0
39	533299.0
40	423.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.396657983430394	21.634700973321756	25.045505244456717	10.923135798791128
2	32.0291524216783	24.244334400269338	25.539856296678447	18.186656881373914
3	31.519602678360574	23.947747989957964	26.561196227818083	17.971453103863375
4	28.903429575562928	26.50669892906753	25.581334796283038	19.008536699086502
5	25.185866065016487	29.694124827879364	24.9298498659972	20.19015924110695
6	25.835776628893985	34.35733757685633	24.743771867041122	15.063113927208564
7	79.82001358799316	6.570799862909017	8.178288518387994	5.430898030709833
8	79.19274967937423	5.224656031216052	8.655684855442887	6.926909433966834
9	73.85152998138615	7.745216650983555	11.207853908485713	7.195399459144586
10	38.97701303938702	26.66534662098582	18.85327995020158	15.504360389425587
11	31.395288284655138	25.570738099303767	23.12816916930373	19.905804446737367
12	28.62991368838929	22.655980351907225	28.98105795000539	19.7330480096981
13	25.05210547286095	24.579190024814437	30.940175287533805	19.42852921479081
14	20.4001554989591	28.711417426298457	29.103192451760805	21.78523462298163
15	19.17560119603405	26.84452162876682	34.67614677454709	19.30373040065203
16	21.397637723756826	26.639006259923047	30.861820282441332	21.10153573387879
17	21.2973626940558	26.173417670930775	28.204410890255758	24.32480874475766
18	21.820355104398658	26.816788558958205	30.007726505911748	21.35512983073139
19	22.76545936984168	28.052363426741707	27.519622055177912	21.662555148238706
20	24.28696339840311	28.32303334386948	27.116826464857112	20.273176792870302
21	22.990775423898153	28.34247071375718	28.473990861408527	20.192763000936143
22	20.98884743057347	26.917366351430072	27.13656659751565	24.95721962048081
23	20.701162245724685	27.577631402070168	27.897833308506787	23.823373043698357
24	22.643264315532093	25.439399609314922	28.487372975879495	23.42996309927349
25	20.580844320594288	26.88569736560058	27.49394777221098	25.039510541594158
26	20.86114209383466	27.866588190556467	27.89686446764011	23.375405247968764
27	23.429660336502653	26.734194875074024	27.382470519989006	22.453674268434316
28	20.58496189427766	28.16668664900954	27.77684930528055	23.47150215143225
29	21.665340565730403	26.460012909804547	26.72250823211974	25.15213829234531
30	25.86793003515681	26.506517271405027	25.454234985085904	22.171317708352255
31	24.42708200874621	27.754626517901155	25.343968783947275	22.474322689405362
32	23.84474809531941	28.6956132096608	25.065245377115254	22.394393317904544
33	26.101178473809206	27.523255208427948	24.99451999384786	21.38104632391499
34	22.96697827011041	25.52429429025746	28.580502804188786	22.928224635443346
35	22.03580109212587	26.140961501897113	27.53585013969474	24.28738726628228
36	24.689819541278073	27.49461385030682	26.241539294368977	21.574027314046134
37	23.86018899663207	28.117517975025702	28.368750522265778	19.653542506076448
38	22.62618849525692	29.04930067855192	26.165848601659867	22.15866222453129
39	24.034762010296358	26.354530360445132	27.864832166485616	21.745875462772897
40	23.287967359751203	24.101006504555368	30.666962163131	21.94406397256243
41	20.4653100472431	25.820275175027156	29.18063916854077	24.53377560918897
42	22.453008190338476	25.897116366265443	28.557492833605213	23.092382609790864
43	23.961856735078943	25.476942192898637	27.203477169870503	23.357723902151918
44	23.118056892757792	25.80283603942698	26.07871347621314	25.000393591602087
45	22.885595637309578	23.774446579931187	26.960358664888844	26.379599117870388
46	23.848139038352777	24.368769899083112	28.560702118976078	23.22238894358803
47	20.434549349726122	25.242967123596237	31.049048779926586	23.273434746751054
48	21.677329971455524	24.004061865333544	29.620311264349443	24.69829689886149
49	22.562608313381265	22.682502370632495	31.08804462481032	23.666844691175918
50	21.35797580077725	22.65071227969467	30.368438071086278	25.622873848441802
51	20.263488384203534	22.734940882541366	28.00125707102451	29.000313662230585
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	823.0
1	1301.5
2	1780.0
3	2523.5
4	3267.0
5	2569.5
6	1872.0
7	2016.0
8	2160.0
9	2335.5
10	2511.0
11	2606.5
12	2702.0
13	2809.0
14	2916.0
15	2953.0
16	2990.0
17	3575.5
18	4161.0
19	4001.5
20	3842.0
21	4118.5
22	4395.0
23	5251.5
24	6108.0
25	7231.0
26	10852.5
27	13351.0
28	14838.5
29	16326.0
30	19953.5
31	23581.0
32	28462.0
33	33343.0
34	37248.5
35	41154.0
36	44321.0
37	47488.0
38	50283.0
39	53078.0
40	61106.5
41	69135.0
42	81126.5
43	93118.0
44	104771.5
45	116425.0
46	134251.5
47	152078.0
48	173549.0
49	195020.0
50	192315.5
51	189611.0
52	161849.5
53	134088.0
54	116809.0
55	99530.0
56	89340.5
57	79151.0
58	72980.5
59	66810.0
60	62804.0
61	58798.0
62	51689.5
63	44581.0
64	36740.5
65	28900.0
66	22638.5
67	16377.0
68	13622.0
69	10867.0
70	9287.0
71	7707.0
72	6401.5
73	5096.0
74	4407.5
75	2954.0
76	2189.0
77	1616.5
78	1044.0
79	826.0
80	608.0
81	431.0
82	254.0
83	174.0
84	94.0
85	57.5
86	21.0
87	22.0
88	23.0
89	14.5
90	6.0
91	5.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1651458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.51335830432671
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.32706626947937	14.054045836258055
2	13.002163707404849	5.59440413137827
3	6.007084512926094	3.87697684472853
4	3.3724341480600972	2.9020953673985472
5	2.152066408037403	2.3149087865407014
6	1.4868814346024801	1.919268783519269
7	1.03672633287246	1.5612425543831143
8	0.7951366910973136	1.3684848429194603
9	0.6036841818129987	1.1688546695397615
>10	4.152946346933235	18.996279322891688
>50	1.2970830708428562	20.19551399190937
>100	0.7544797724381216	22.558634789109906
>500	0.008259687937090525	1.1733906687204834
>1k	0.0031329850795860613	1.0555614125351314
>5k	8.54450476250744E-4	1.2603379981677088
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	8307	0.5030100674676559	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	7102	0.43004423969607464	TruSeq Adapter, Index 22 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGCT	5160	0.3124511795032026	Illumina Paired End PCR Primer 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	3093	0.1872890500394197	TruSeq Adapter, Index 22 (95% over 22bp)
ACTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTGC	2364	0.14314623805146723	No Hit
GCCTGTCTCTTATACACATCTGACGCCGGAGTTATCGTATGCCGTCTTCTG	1658	0.10039613480936238	TruSeq Adapter, Index 22 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.81657662501862E-4	0.0	0.0	0.3258332939741731	0.0
2	1.81657662501862E-4	0.0	0.0	1.65496185794613	0.0
3	1.81657662501862E-4	0.0	0.0	2.3168012749945803	0.0
4	1.81657662501862E-4	0.0	0.0	3.134624071577963	0.0
5	1.81657662501862E-4	0.0	0.0	5.2585654615497335	0.0
6	1.81657662501862E-4	0.0	0.0	6.411607198003219	0.0
7	1.81657662501862E-4	0.0	0.0	7.5398829398022835	0.0
8	1.81657662501862E-4	0.0	0.0	9.30117508286617	0.0
9	1.81657662501862E-4	0.0	0.0	9.928257333822598	0.0
10	1.81657662501862E-4	0.0	0.0	11.446733734675663	0.0
11	1.81657662501862E-4	0.0	0.0	13.634860832064758	0.0
12	1.81657662501862E-4	0.0	0.0	15.252340658981337	0.0
13	1.81657662501862E-4	0.0	0.0	15.898618069608794	0.0
14	2.422102166691493E-4	0.0	0.0	16.156148082482268	0.0
15	2.422102166691493E-4	0.0	0.0	16.63372607719966	0.0
16	2.422102166691493E-4	0.0	0.0	17.662271762285204	0.0
17	2.422102166691493E-4	0.0	0.0	18.843530988980646	0.0
18	2.422102166691493E-4	0.0	0.0	20.186465535302744	0.0
19	2.422102166691493E-4	0.0	0.0	20.973345976706643	0.0
20	2.422102166691493E-4	0.0	0.0	21.746299330652068	0.0
21	2.422102166691493E-4	0.0	0.0	22.683895079378342	0.0
22	2.422102166691493E-4	0.0	0.0	23.666965796284252	0.0
23	2.422102166691493E-4	0.0	0.0	24.62841925135244	0.0
24	2.422102166691493E-4	0.0	0.0	25.34384767883894	0.0
25	2.422102166691493E-4	0.0	0.0	26.008109198054083	0.0
26	2.422102166691493E-4	0.0	0.0	26.58941371806004	0.0
27	2.422102166691493E-4	0.0	0.0	27.17538078473688	0.0
28	2.422102166691493E-4	0.0	0.0	27.777939251255557	0.0
29	3.0276277083643666E-4	0.0	0.0	28.387582366611806	0.0
30	3.0276277083643666E-4	0.0	0.0	29.07836590455222	0.0
31	3.63315325003724E-4	0.0	0.0	29.723190053879662	0.0
32	4.238678791710113E-4	0.0	0.0	30.394536221932377	0.0
33	4.238678791710113E-4	0.0	0.0	31.028703121726377	0.0
34	4.238678791710113E-4	0.0	0.0	31.604981779736452	0.0
35	4.238678791710113E-4	0.0	0.0	32.227401484021996	0.0
36	4.238678791710113E-4	0.0	0.0	32.831110449069854	0.0
37	4.238678791710113E-4	0.0	0.0	33.43179178640934	0.0
38	4.238678791710113E-4	0.0	0.0	34.0095842582736	0.0
39	4.238678791710113E-4	0.0	0.0	34.6306112538133	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGC	20	7.0341816E-4	45.000004	20
CGTTGTC	20	7.0341816E-4	45.000004	22
CGGCATA	60	0.0	45.000004	23
CGTTCGC	20	7.0341816E-4	45.000004	28
TAACACG	20	7.0341816E-4	45.000004	1
GTCGTTA	40	6.8175723E-9	45.000004	31
TTACGCG	20	7.0341816E-4	45.000004	1
CGTCGAA	20	7.0341816E-4	45.000004	42
CACGTAA	20	7.0341816E-4	45.000004	29
GCTCGTC	30	2.1660799E-6	45.000004	9
ATCGCCT	20	7.0341816E-4	45.000004	31
ATCGCCC	20	7.0341816E-4	45.000004	16
TAGTCGT	20	7.0341816E-4	45.000004	15
CCGCAAT	20	7.0341816E-4	45.000004	26
CTTACGA	20	7.0341816E-4	45.000004	29
TATCCCG	20	7.0341816E-4	45.000004	10
CTGTCGT	35	1.2123564E-7	45.0	37
GATCGCG	25	3.8915496E-5	45.0	1
CCTACGG	25	3.8915496E-5	45.0	2
TTACTCG	25	3.8915496E-5	45.0	35
>>END_MODULE