FastQCFastQC Report
Sat 14 Jan 2017
SRR2935005.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935005.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582059
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC76671.3172204192358508No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC69681.197129500617635No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT51500.8847900298766963No Hit
TCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC28410.48809484949120274No Hit
ACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC26900.4621524622074395No Hit
AGTACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT20170.34652844471093136No Hit
AGTACTGAATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCC19070.32763001688832233No Hit
AGTACTGAATGATACCTGTCTCTTATACACATCTGACGCTTGGTACATCGT16730.28742790679295394No Hit
CGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG15470.2657806167416018No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14090.24207168001869225No Hit
AAAACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC13770.23657395556120597No Hit
AGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG13410.23038901554653393No Hit
GCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG11950.20530564770925283No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCC11410.19602823768724476No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC10450.17953506431478597No Hit
AGACATAGACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCC10150.1743809476358926No Hit
ACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG9640.16561894928177384No Hit
GGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG9360.16080844038147335No Hit
AGTACTGAATGATACGGCTGTCTCTTATACACATCTGACGCTTGGTACATC7240.12438601585062684No Hit
AGCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT7160.12301158473625527No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT6900.11854468361454766No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6900.11854468361454766No Hit
GGCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT6890.11837287972525122No Hit
GAAACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC6600.11339056693565427No Hit
TGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG6120.1051439802494249No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGT253.8884238E-545.0000049
TGATCGC351.2105374E-745.00000414
GTTTGCG351.2105374E-745.0000041
TATCACT253.8884238E-545.0000048
TCTGACA253.8884238E-545.00000444
CTGTCCA351.2105374E-745.00000411
TCTTGCG253.8884238E-545.0000041
ACTGCGT253.8884238E-545.00000426
GATCGTC253.8884238E-545.00000428
CACGGTG253.8884238E-545.00000410
ATAGGCG253.8884238E-545.0000044
CCTAGTA351.2105374E-745.00000427
TTAGCTA253.8884238E-545.00000442
CGAAACG253.8884238E-545.00000440
CCCTATT253.8884238E-545.00000434
CGTGCGG253.8884238E-545.0000042
TTAGCCG253.8884238E-545.0000041
TAGACCG253.8884238E-545.0000041
GACGTTG253.8884238E-545.00000436
CATTGCC253.8884238E-545.00000434
Produced by FastQC (version 0.11.5)