##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935005.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	582059
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08946515731223	33.0	31.0	34.0	30.0	34.0
2	32.29373139149124	34.0	31.0	34.0	30.0	34.0
3	32.26592493200861	34.0	31.0	34.0	30.0	34.0
4	35.869303627295515	37.0	35.0	37.0	35.0	37.0
5	35.76946151506978	37.0	35.0	37.0	35.0	37.0
6	35.72114682532183	37.0	35.0	37.0	35.0	37.0
7	36.002374329750076	37.0	35.0	37.0	35.0	37.0
8	35.68498726074161	37.0	35.0	37.0	35.0	37.0
9	37.37757856162348	39.0	37.0	39.0	34.0	39.0
10	37.37106891225804	39.0	37.0	39.0	34.0	39.0
11	37.29300294300062	39.0	37.0	39.0	34.0	39.0
12	37.06599159191766	39.0	35.0	39.0	34.0	39.0
13	36.862828338707935	39.0	35.0	39.0	33.0	39.0
14	37.84411717712466	40.0	36.0	41.0	33.0	41.0
15	37.98002092571372	40.0	36.0	41.0	33.0	41.0
16	38.07127799759131	40.0	36.0	41.0	34.0	41.0
17	38.10729668298231	40.0	36.0	41.0	34.0	41.0
18	38.107042413226154	40.0	36.0	41.0	34.0	41.0
19	38.0450297993846	40.0	36.0	41.0	34.0	41.0
20	38.00738928527864	40.0	36.0	41.0	34.0	41.0
21	37.931223467036844	40.0	36.0	41.0	34.0	41.0
22	37.84440408961978	39.0	35.0	41.0	34.0	41.0
23	37.63079172386305	39.0	35.0	41.0	33.0	41.0
24	37.42473013904089	39.0	35.0	41.0	33.0	41.0
25	37.379696903578505	39.0	35.0	41.0	33.0	41.0
26	37.56655253161621	39.0	35.0	41.0	33.0	41.0
27	37.65321213141623	39.0	35.0	41.0	33.0	41.0
28	37.67674239209427	39.0	35.0	41.0	33.0	41.0
29	37.70781140743464	39.0	36.0	41.0	34.0	41.0
30	37.49726745914074	39.0	36.0	41.0	33.0	41.0
31	37.35069812510416	39.0	35.0	41.0	33.0	41.0
32	37.26870128285964	39.0	35.0	41.0	33.0	41.0
33	37.07272974045586	39.0	35.0	41.0	33.0	41.0
34	36.99851217831869	39.0	35.0	41.0	33.0	41.0
35	36.9537005698735	39.0	35.0	41.0	32.0	41.0
36	36.816376003119956	39.0	35.0	41.0	32.0	41.0
37	36.773184505350834	39.0	35.0	41.0	32.0	41.0
38	36.697687004238404	39.0	35.0	41.0	32.0	41.0
39	36.5805476764383	39.0	35.0	40.0	31.0	41.0
40	36.52725410997854	39.0	35.0	40.0	31.0	41.0
41	36.56895950410525	39.0	35.0	40.0	31.0	41.0
42	36.62701032025963	39.0	35.0	41.0	32.0	41.0
43	36.57289037709236	39.0	35.0	41.0	31.0	41.0
44	36.376367344203935	39.0	35.0	40.0	31.0	41.0
45	36.406838482009555	39.0	35.0	40.0	31.0	41.0
46	36.26911189415506	39.0	35.0	40.0	31.0	41.0
47	36.22219053394931	38.0	35.0	40.0	31.0	41.0
48	36.23818032192613	39.0	35.0	40.0	31.0	41.0
49	36.22995263366772	39.0	35.0	40.0	31.0	41.0
50	36.16686109140139	39.0	35.0	40.0	31.0	41.0
51	35.15736205436219	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	4.0
10	9.0
11	11.0
12	8.0
13	17.0
14	18.0
15	16.0
16	37.0
17	84.0
18	180.0
19	317.0
20	570.0
21	917.0
22	1554.0
23	2293.0
24	3164.0
25	3738.0
26	4028.0
27	4379.0
28	4881.0
29	5848.0
30	7343.0
31	9591.0
32	13653.0
33	19665.0
34	39590.0
35	61224.0
36	42991.0
37	67782.0
38	131829.0
39	156231.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.2163303032854	25.504974581614583	25.418729029187766	11.859966085912253
2	32.249479863725156	24.916374456884956	23.910462685054267	18.923682994335625
3	28.985205967092682	24.87651595456818	29.199101809266757	16.93917626907238
4	28.85085532566286	28.084094567732826	25.770239786688293	17.294810319916024
5	23.33646589091484	29.095504064021	25.843771851307167	21.72425819375699
6	22.623995162002476	31.2222644096217	27.581911799319315	18.571828629056505
7	72.33854299993642	7.823605510781554	12.386201398827266	7.451650090454748
8	69.34434481727797	6.896208116359339	12.30923325642246	11.45021380994023
9	62.519950726644545	9.42567677847091	17.530525256030746	10.523847238853794
10	37.57643125525076	23.70859311513094	23.44848202673612	15.26649360288218
11	29.62517545472194	24.06577340097825	25.669562707560573	20.63948843673923
12	26.394059708723688	21.697972198694636	32.83137963677222	19.076588455809464
13	21.321206269467528	29.370905698563206	32.7741689416365	16.533719090332767
14	14.598691885186895	34.21508816116579	30.020152596214473	21.166067357432837
15	11.982977670648507	27.427975514509694	41.249254800630176	19.339792014211618
16	13.117570555562235	27.17198771945799	36.2353300954027	23.475111629577068
17	13.709091346409899	26.981800814006828	28.150239065111954	31.158868774471316
18	15.228696747236963	26.405055157638657	35.3601610833266	23.006087011797774
19	18.385593213059156	27.891674211720808	29.91432140040786	23.808411174812175
20	20.80785624824975	28.73746475872721	28.897585983551494	21.557093009471547
21	18.237154652707027	29.64854078366626	31.81498782769444	20.29931673593227
22	15.17767099211592	29.53927351007372	26.256960205065123	29.026095292745236
23	15.502723950664796	29.009945727151372	27.99303850640571	27.494291815778126
24	20.38762393503064	24.864317878428132	30.148146493740324	24.5999116928009
25	15.641541493216323	28.481476963675505	28.166904042373712	27.71007750073446
26	14.820490706268608	30.253118670100456	32.18024289633869	22.74614772729225
27	18.85100994916323	29.37794965802436	31.368297715523685	20.402742677288728
28	18.44606818209151	25.634858321922692	31.960677525817832	23.958395970167974
29	19.61433462930734	22.97825478173175	33.63043952588999	23.776971063070924
30	20.920215991849624	25.053130352764924	33.345588677436474	20.68106497794897
31	23.405874662190605	30.43265373441524	24.739416450909616	21.422055152484543
32	23.933140798441396	26.807763474149525	25.904246820339523	23.35484890706956
33	22.156173171448255	28.17961753018165	27.814706069315996	21.849503229054097
34	19.972202130711835	26.242185070585627	30.31118838468265	23.474424414019886
35	18.69380939045698	22.72879553447331	30.245559298971408	28.331835776098302
36	21.82923037011712	26.490441690618994	32.58174858562448	19.09857935363941
37	22.336051843541636	27.231603669043857	31.727883255821144	18.70446123159336
38	22.84184249363037	26.970977168981154	25.837586911292497	24.349593426095982
39	22.166309600916744	25.88002247194872	30.91988956445996	21.033778362674575
40	22.083156518497265	23.998941688041935	32.197766893046925	21.720134900413875
41	16.092870310398087	25.826247854598932	27.695989581812153	30.384892253190827
42	21.37858876849254	23.927299466205316	29.53978892174161	25.154322843560532
43	25.424913969202436	22.06941220735355	30.553260064701345	21.95241375874267
44	22.137446547514944	22.310968475704353	29.899889873706957	25.651695103073745
45	22.74717855062803	21.078275570002354	27.831199242688452	28.34334663668116
46	23.411715994426682	21.57925571119079	32.28916656215263	22.719861732229894
47	18.155032393623326	23.99292855191656	34.414380672749665	23.437658381710445
48	18.24866551328989	19.477063321759477	33.31260233069156	28.96166883425907
49	21.333404345607576	18.308796874543646	36.61621931797292	23.741579461875858
50	20.913343836277765	18.448473436541658	33.53594738677694	27.102235340403634
51	16.99501253309372	18.996527843397317	28.416706897410744	35.591752726098214
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1774.0
1	1648.0
2	1522.0
3	1720.5
4	1919.0
5	1569.5
6	1220.0
7	1318.0
8	1416.0
9	1653.0
10	1890.0
11	1967.5
12	2045.0
13	2036.5
14	2028.0
15	2101.0
16	2174.0
17	2211.0
18	2248.0
19	2079.0
20	1910.0
21	1799.0
22	1688.0
23	1911.0
24	2134.0
25	2051.0
26	2712.0
27	3456.0
28	3478.0
29	3500.0
30	4421.0
31	5342.0
32	6308.5
33	7275.0
34	8680.0
35	10085.0
36	10119.0
37	10153.0
38	12525.5
39	14898.0
40	21956.0
41	29014.0
42	38742.0
43	48470.0
44	63006.5
45	77543.0
46	89403.5
47	101264.0
48	96712.0
49	92160.0
50	70285.5
51	48411.0
52	39187.0
53	29963.0
54	25181.0
55	20399.0
56	16889.0
57	13379.0
58	11756.0
59	10133.0
60	10525.5
61	10918.0
62	9419.5
63	7921.0
64	6186.0
65	4451.0
66	3757.0
67	3063.0
68	2480.5
69	1898.0
70	1489.5
71	1081.0
72	786.5
73	492.0
74	481.5
75	297.0
76	123.0
77	100.5
78	78.0
79	95.0
80	112.0
81	86.0
82	60.0
83	32.5
84	5.0
85	2.5
86	0.0
87	2.0
88	4.0
89	2.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	582059.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.403677668682015
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.57998127840399	15.11409065689286
2	12.309186951683863	5.761604875615111
3	6.15455261866074	4.321174970462362
4	3.739453561245799	3.5006786321760694
5	2.554675215014713	2.9894397640187633
6	1.6998964618145735	2.3870297317464777
7	1.2528340174931905	2.0524646462439384
8	0.9381177072862127	1.7564323549287604
9	0.754012955692312	1.588200855572977
>10	4.622445957748562	21.429319972855367
>50	0.9872448166311674	16.659735888130523
>100	0.3861847712027678	13.829051429684439
>500	0.00959923905475331	1.5283622314100842
>1k	0.00959923905475331	3.663299703915758
>5k	0.0022152090126353794	3.419114286346508
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	7667	1.3172204192358508	No Hit
GCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	6968	1.197129500617635	No Hit
CTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGCT	5150	0.8847900298766963	No Hit
TCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	2841	0.48809484949120274	No Hit
ACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTGC	2690	0.4621524622074395	No Hit
AGTACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT	2017	0.34652844471093136	No Hit
AGTACTGAATCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCC	1907	0.32763001688832233	No Hit
AGTACTGAATGATACCTGTCTCTTATACACATCTGACGCTTGGTACATCGT	1673	0.28742790679295394	No Hit
CGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG	1547	0.2657806167416018	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1409	0.24207168001869225	No Hit
AAAACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC	1377	0.23657395556120597	No Hit
AGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG	1341	0.23038901554653393	No Hit
GCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG	1195	0.20530564770925283	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCC	1141	0.19602823768724476	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC	1045	0.17953506431478597	No Hit
AGACATAGACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCC	1015	0.1743809476358926	No Hit
ACCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG	964	0.16561894928177384	No Hit
GGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG	936	0.16080844038147335	No Hit
AGTACTGAATGATACGGCTGTCTCTTATACACATCTGACGCTTGGTACATC	724	0.12438601585062684	No Hit
AGCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT	716	0.12301158473625527	No Hit
AAAAACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTT	690	0.11854468361454766	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	690	0.11854468361454766	No Hit
GGCCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCT	689	0.11837287972525122	No Hit
GAAACTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTC	660	0.11339056693565427	No Hit
TGCTGTCTCTTATACACATCTGACGCTTGGTACATCGTATGCCGTCTTCTG	612	0.1051439802494249	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.914340298835685	0.0
2	0.0	0.0	0.0	4.496451390666582	0.0
3	0.0	0.0	0.0	6.062615645492983	0.0
4	0.0	0.0	0.0	7.7651921884207615	0.0
5	0.0	0.0	0.0	12.107879098167023	0.0
6	0.0	0.0	0.0	14.73046546827727	0.0
7	0.0	0.0	0.0	17.12146019561591	0.0
8	0.0	0.0	0.0	20.64034745618571	0.0
9	0.0	0.0	0.0	21.906542120300518	0.0
10	0.0	0.0	0.0	24.43463635129772	0.0
11	0.0	0.0	0.0	28.794160042195035	0.0
12	0.0	0.0	0.0	31.879242482291314	0.0
13	0.0	0.0	0.0	33.051975830628855	0.0
14	0.0	0.0	0.0	33.50674072559654	0.0
15	0.0	0.0	0.0	34.27710936520181	0.0
16	0.0	0.0	0.0	36.32466811783685	0.0
17	0.0	0.0	0.0	38.307972215875026	0.0
18	0.0	0.0	0.0	40.94017960378587	0.0
19	0.0	0.0	0.0	42.106899815998034	0.0
20	0.0	0.0	0.0	43.16847604796077	0.0
21	0.0	0.0	0.0	44.496691916111594	0.0
22	0.0	0.0	0.0	45.61616605876724	0.0
23	0.0	0.0	0.0	46.796286974344525	0.0
24	0.0	0.0	0.0	47.63778242411852	0.0
25	0.0	0.0	0.0	48.404886789827145	0.0
26	0.0	0.0	0.0	49.08608921088756	0.0
27	0.0	0.0	0.0	49.728292149077674	0.0
28	0.0	0.0	0.0	50.343006464980355	0.0
29	0.0	0.0	0.0	50.951879448646956	0.0
30	0.0	0.0	0.0	51.735305183838754	0.0
31	0.0	0.0	0.0	52.468048771688096	0.0
32	0.0	0.0	0.0	53.065754502550426	0.0
33	0.0	0.0	0.0	53.59559769714067	0.0
34	0.0	0.0	0.0	54.14708818178226	0.0
35	0.0	0.0	0.0	54.80801774390569	0.0
36	0.0	0.0	0.0	55.40005394642124	0.0
37	0.0	0.0	0.0	55.943469648265896	0.0
38	0.0	0.0	0.0	56.44444978945433	0.0
39	0.0	0.0	0.0	56.999719959660446	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGT	25	3.8884238E-5	45.000004	9
TGATCGC	35	1.2105374E-7	45.000004	14
GTTTGCG	35	1.2105374E-7	45.000004	1
TATCACT	25	3.8884238E-5	45.000004	8
TCTGACA	25	3.8884238E-5	45.000004	44
CTGTCCA	35	1.2105374E-7	45.000004	11
TCTTGCG	25	3.8884238E-5	45.000004	1
ACTGCGT	25	3.8884238E-5	45.000004	26
GATCGTC	25	3.8884238E-5	45.000004	28
CACGGTG	25	3.8884238E-5	45.000004	10
ATAGGCG	25	3.8884238E-5	45.000004	4
CCTAGTA	35	1.2105374E-7	45.000004	27
TTAGCTA	25	3.8884238E-5	45.000004	42
CGAAACG	25	3.8884238E-5	45.000004	40
CCCTATT	25	3.8884238E-5	45.000004	34
CGTGCGG	25	3.8884238E-5	45.000004	2
TTAGCCG	25	3.8884238E-5	45.000004	1
TAGACCG	25	3.8884238E-5	45.000004	1
GACGTTG	25	3.8884238E-5	45.000004	36
CATTGCC	25	3.8884238E-5	45.000004	34
>>END_MODULE