##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2935004.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	601454
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98070675396622	33.0	31.0	34.0	30.0	34.0
2	32.150791249206094	33.0	31.0	34.0	30.0	34.0
3	32.174483834175184	34.0	31.0	34.0	30.0	34.0
4	35.782851223867496	37.0	35.0	37.0	35.0	37.0
5	35.674716603431015	37.0	35.0	37.0	33.0	37.0
6	35.592161661573456	37.0	35.0	37.0	33.0	37.0
7	35.89934558586359	37.0	35.0	37.0	35.0	37.0
8	35.557469066628535	37.0	35.0	37.0	35.0	37.0
9	37.32737333195889	39.0	37.0	39.0	34.0	39.0
10	37.25293538657986	39.0	37.0	39.0	34.0	39.0
11	37.14447655182275	39.0	37.0	39.0	34.0	39.0
12	36.95188326954347	39.0	35.0	39.0	33.0	39.0
13	36.698570464241655	39.0	35.0	39.0	33.0	39.0
14	37.686178826643435	39.0	36.0	41.0	33.0	41.0
15	37.78283293485453	39.0	36.0	41.0	33.0	41.0
16	37.9283868758043	40.0	36.0	41.0	33.0	41.0
17	37.94644977005723	39.0	36.0	41.0	34.0	41.0
18	37.979245295567075	39.0	36.0	41.0	34.0	41.0
19	37.932197641049854	39.0	36.0	41.0	34.0	41.0
20	37.85710627911694	39.0	36.0	41.0	34.0	41.0
21	37.79308642057414	39.0	35.0	41.0	34.0	41.0
22	37.67682981574651	39.0	35.0	41.0	33.0	41.0
23	37.44285681032964	39.0	35.0	41.0	33.0	41.0
24	37.2080757630675	39.0	35.0	41.0	32.0	41.0
25	37.188307002696796	39.0	35.0	41.0	32.0	41.0
26	37.40017856727198	39.0	35.0	41.0	33.0	41.0
27	37.372079992817405	39.0	35.0	41.0	33.0	41.0
28	37.40155190588141	39.0	35.0	41.0	33.0	41.0
29	37.43607823707216	39.0	35.0	41.0	33.0	41.0
30	37.30027400266687	39.0	35.0	41.0	33.0	41.0
31	37.16255274717601	39.0	35.0	41.0	33.0	41.0
32	36.95435228629288	39.0	35.0	41.0	32.0	41.0
33	36.850838135584766	39.0	35.0	41.0	32.0	41.0
34	36.79298333704656	39.0	35.0	41.0	32.0	41.0
35	36.61518586625078	39.0	35.0	40.0	31.0	41.0
36	36.55293172877726	39.0	35.0	40.0	31.0	41.0
37	36.392623542282536	39.0	35.0	40.0	31.0	41.0
38	36.350118213529214	38.0	35.0	40.0	31.0	41.0
39	36.34984387833484	39.0	35.0	40.0	31.0	41.0
40	36.261128864385306	38.0	35.0	40.0	31.0	41.0
41	36.172972496649784	38.0	35.0	40.0	30.0	41.0
42	36.186428222274685	38.0	35.0	40.0	31.0	41.0
43	36.061125206582716	38.0	35.0	40.0	30.0	41.0
44	35.93276460045157	38.0	35.0	40.0	30.0	41.0
45	35.96018149351405	38.0	35.0	40.0	30.0	41.0
46	35.81707994293828	38.0	35.0	40.0	29.0	41.0
47	35.684750620995125	38.0	35.0	40.0	29.0	41.0
48	35.677350221297054	38.0	35.0	40.0	29.0	41.0
49	35.68306969444047	38.0	35.0	40.0	29.0	41.0
50	35.58393659365471	38.0	35.0	40.0	29.0	41.0
51	34.50644272047405	36.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	11.0
11	9.0
12	9.0
13	8.0
14	14.0
15	26.0
16	50.0
17	86.0
18	187.0
19	392.0
20	718.0
21	1169.0
22	1800.0
23	2596.0
24	3456.0
25	4099.0
26	4431.0
27	5012.0
28	5892.0
29	7347.0
30	9162.0
31	11781.0
32	16010.0
33	23296.0
34	43272.0
35	64211.0
36	48845.0
37	76823.0
38	135259.0
39	135398.0
40	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.92789473509196	22.251909539216634	22.993612146564825	12.826583579126583
2	27.602775939639606	27.791319036867325	24.975143568751726	19.630761454741343
3	26.97363389386387	28.43459350174743	26.93472817538831	17.65704442900039
4	26.866560036178992	25.726156946333383	28.716244301309825	18.691038716177797
5	24.99243499918531	30.160078742513974	24.55898539206656	20.28850086623416
6	23.21474293960968	33.50114888254131	26.439927242981177	16.844180934867836
7	70.58245518360506	8.388671452845935	13.649090371000941	7.379782992548059
8	67.63509761345007	7.581128398846794	13.187209661919283	11.59656432578385
9	62.6383730094072	8.255327922002348	16.753733452599867	12.352565615990581
10	37.63912119630096	23.26379074708955	22.199536456653377	16.897551599956106
11	28.724058697755773	23.04282621779887	26.1802232589691	22.052891825476262
12	25.006234890781343	20.445786377678093	32.881151343244866	21.666827388295697
13	20.311944055572	29.42336404779085	34.01440509166121	16.250286804975943
14	15.688149052130337	33.15748170267385	28.115699621251167	23.038669623944642
15	12.421066282708237	26.33468228659216	41.20032454684814	20.04392688385147
16	12.808627093676325	27.263764144888885	34.01706531172791	25.910543449706875
17	13.168089330189838	26.71609133865599	28.536679446807238	31.579139884346937
18	14.937634465811184	26.275159862599633	34.870497161877715	23.916708509711466
19	16.80078609502971	27.973876638944954	29.391940198252904	25.833397067772427
20	19.497417923897757	28.565276812524313	31.040279057084994	20.89702620649293
21	18.758541800370434	30.160245006268145	31.30729864628051	19.773914547080906
22	14.976706448040913	28.69828781585957	26.649253309480024	29.67575242661949
23	14.051614919844244	29.63601538937309	27.95475630721552	28.357613383567156
24	19.399488572692174	24.4647138434527	29.439990423207764	26.695807160647366
25	14.270085492822393	30.195825449660322	27.06757291496939	28.466516142547892
26	18.001376663884518	28.792559364473426	28.40483228975117	24.801231681890883
27	20.31809581447625	31.676570444290004	25.786843216605092	22.21849052462865
28	14.287543187010145	28.20764347730666	29.91749992518131	27.587313410501885
29	19.60648694663266	26.50144483202373	26.202502602027756	27.689565619315857
30	19.57423177832386	31.451948112407603	22.862596308279603	26.111223800988938
31	19.739165422459575	34.00592564019858	18.34404626122696	27.910862676114885
32	20.713969813152794	37.49098019133633	17.673837068171462	24.121212927339414
33	17.19699262121459	35.04025245488433	19.089074143658536	28.673680780242545
34	18.03329930468498	31.066881257752048	23.48109747378852	27.418721963774452
35	17.613317061653923	33.73707714970721	20.000864571521678	28.648741217117184
36	21.226727231010184	32.95015080122503	21.026711934744803	24.79641003301998
37	15.699454987413834	35.24858093885817	26.864398607374795	22.187565466353202
38	14.543755632184672	35.91247210925524	20.506306384195632	29.037465874364454
39	18.703175970232138	32.18400742201399	25.10582687952861	24.006989728225268
40	20.321753617067973	28.943360589504767	25.755585630821308	24.979300162605952
41	17.213618996631496	33.15299258131129	21.473296378442907	28.16009204361431
42	20.935433133705985	28.503094168465086	26.39802877693057	24.163443920898356
43	22.723101018531757	27.460287902316722	26.16276556478135	23.653845514370175
44	18.51446660925025	25.722332879987498	25.903892899540114	29.859307611222135
45	19.28726053862806	24.94920642310135	24.373767569922222	31.38976546834837
46	24.197195462994674	26.335347341608834	26.321713713766968	23.14574348162952
47	15.650407179933959	26.89382729186272	32.28160424571123	25.174161282492097
48	17.641249372354327	25.22038260615109	27.708852214799467	29.42951580669511
49	20.16912349074077	21.074762159699663	33.95455014016034	24.801564209399224
50	20.618035626997244	20.65760640048948	30.28810183322415	28.436256139289124
51	17.443894296155648	20.351681092818403	24.86757091980434	37.336853691221606
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1384.0
1	1363.5
2	1343.0
3	1460.5
4	1578.0
5	1297.5
6	1017.0
7	1154.5
8	1292.0
9	1449.5
10	1607.0
11	1794.5
12	1982.0
13	2039.5
14	2097.0
15	2158.0
16	2219.0
17	2212.0
18	2205.0
19	2140.0
20	2075.0
21	1965.0
22	1855.0
23	1759.5
24	1664.0
25	1500.5
26	2240.0
27	3143.0
28	2597.5
29	2052.0
30	3440.5
31	4829.0
32	5635.0
33	6441.0
34	7427.0
35	8413.0
36	8752.5
37	9092.0
38	11340.0
39	13588.0
40	18798.0
41	24008.0
42	34720.0
43	45432.0
44	66447.0
45	87462.0
46	96771.0
47	106080.0
48	103987.5
49	101895.0
50	78707.5
51	55520.0
52	44780.5
53	34041.0
54	28660.5
55	23280.0
56	19340.0
57	15400.0
58	12988.5
59	10577.0
60	9529.0
61	8481.0
62	6875.0
63	5269.0
64	4666.0
65	4063.0
66	3632.5
67	3202.0
68	2570.0
69	1938.0
70	1677.0
71	1416.0
72	1235.5
73	1055.0
74	775.0
75	358.5
76	222.0
77	245.0
78	268.0
79	151.0
80	34.0
81	63.5
82	93.0
83	50.0
84	7.0
85	4.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	601454.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.469749709464935
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.47302209265192	15.366354412368086
2	11.943024504542509	5.605995917912383
3	5.96900584694177	4.202732197261684
4	3.6435703413691045	3.4205473584305044
5	2.401376660350204	2.817985458828504
6	1.6368849167995791	2.3050367580290674
7	1.2274924352342584	2.016625815764675
8	0.9363157426899202	1.758007690397136
9	0.7767423735607354	1.6406954186585558
>10	4.622204327944767	20.976901195879183
>50	0.9464053984351847	15.96526221567974
>100	0.39897482483051905	13.93486694014171
>500	0.012847132105454996	1.988685150663105
>1k	0.00999221385979833	4.370015010992174
>5k	0.002141188684242499	3.6302884589934985
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	8718	1.4494874088458967	No Hit
GCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	7775	1.2927006886644699	No Hit
CTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGCT	5179	0.8610799828415805	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGC	3985	0.6625610603637186	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC	3878	0.6447708386676289	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCG	3528	0.5865785247084565	No Hit
TCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	2402	0.3993655375140908	No Hit
ACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTGC	1904	0.31656618793789715	No Hit
CGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG	1510	0.2510582687952861	No Hit
GCCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG	1274	0.2118200228113871	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1193	0.1983526587236929	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC	1136	0.1888756247360563	No Hit
ACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG	1121	0.18638166842352033	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCC	1111	0.1847190308818297	No Hit
GGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG	1031	0.17141793054830462	No Hit
AGACATAGACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCC	1009	0.1677601279565852	No Hit
AAAACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC	1006	0.16726133669407803	No Hit
AGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCTG	961	0.15977946775647017	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCT	909	0.15113375253967887	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGACACAAAT	903	0.15013617001466445	No Hit
GGCCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT	773	0.12852188197268619	No Hit
AGGTACAGGAGCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGC	722	0.12004243051006394	No Hit
GACCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT	660	0.109734077751582	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTC	658	0.10940155024324388	No Hit
GAAACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTC	657	0.1092352864890748	No Hit
GAACTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT	645	0.10724012143904604	No Hit
AGCCTGTCTCTTATACACATCTGACGCGACACAAATCGTATGCCGTCTTCT	603	0.10025704376394537	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9046410864338752	0.0
2	0.0	0.0	0.0	4.554629281707329	0.0
3	0.0	0.0	0.0	6.034044166303658	0.0
4	0.0	0.0	0.0	7.725279073711373	0.0
5	0.0	0.0	0.0	12.499043983413529	0.0
6	0.0	0.0	0.0	14.590974538368686	0.0
7	0.0	0.0	0.0	17.09041755479222	0.0
8	0.0	0.0	0.0	20.981820721119156	0.0
9	0.0	0.0	0.0	22.349672626668042	0.0
10	0.0	0.0	0.0	25.806296075842873	0.0
11	0.0	0.0	0.0	30.03272070682047	0.0
12	0.0	0.0	0.0	33.99046311106086	0.0
13	1.6626375416906364E-4	0.0	0.0	35.208345110349256	0.0
14	1.6626375416906364E-4	0.0	0.0	35.67587878707266	0.0
15	1.6626375416906364E-4	0.0	0.0	36.619259328227926	0.0
16	1.6626375416906364E-4	0.0	0.0	38.299853355368825	0.0
17	1.6626375416906364E-4	0.0	0.0	40.31812906722708	0.0
18	1.6626375416906364E-4	0.0	0.0	42.92630857887719	0.0
19	1.6626375416906364E-4	0.0	0.0	44.30214114462619	0.0
20	1.6626375416906364E-4	0.0	0.0	45.364234006258165	0.0
21	1.6626375416906364E-4	0.0	0.0	46.634156560601475	0.0
22	3.325275083381273E-4	0.0	0.0	47.770403056592855	0.0
23	3.325275083381273E-4	0.0	0.0	48.957027470097465	0.0
24	3.325275083381273E-4	0.0	0.0	49.89874537371104	0.0
25	3.325275083381273E-4	0.0	0.0	50.64327446488011	0.0
26	3.325275083381273E-4	0.0	0.0	51.357876080298745	0.0
27	3.325275083381273E-4	0.0	0.0	51.98219647720358	0.0
28	3.325275083381273E-4	0.0	0.0	52.58373873978725	0.0
29	3.325275083381273E-4	0.0	0.0	53.25627562540111	0.0
30	3.325275083381273E-4	0.0	0.0	54.09557505644654	0.0
31	6.650550166762546E-4	0.0	0.0	54.84642217027404	0.0
32	6.650550166762546E-4	0.0	0.0	55.42635014481573	0.0
33	6.650550166762546E-4	0.0	0.0	56.03437669381199	0.0
34	6.650550166762546E-4	0.0	0.0	56.58321334632407	0.0
35	6.650550166762546E-4	0.0	0.0	57.203210885620514	0.0
36	6.650550166762546E-4	0.0	0.0	57.815726555979346	0.0
37	6.650550166762546E-4	0.0	0.0	58.34178507417026	0.0
38	6.650550166762546E-4	0.0	0.0	58.86667974608199	0.0
39	6.650550166762546E-4	0.0	0.0	59.337372434134615	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGC	30	2.1637698E-6	45.000004	19
TCCGGAC	25	3.8885795E-5	45.000004	32
ATCCTTA	25	3.8885795E-5	45.000004	29
ACAACGA	25	3.8885795E-5	45.000004	13
TTGATCG	25	3.8885795E-5	45.000004	1
CTTCGCT	30	2.1637698E-6	45.000004	24
AGGTATC	25	3.8885795E-5	45.000004	19
CGTGGCA	25	3.8885795E-5	45.000004	10
TCGTTCT	45	3.8380676E-10	45.000004	29
CTCACGA	30	2.1637698E-6	45.000004	24
GCCTTCG	30	2.1637698E-6	45.000004	22
TCGCAAC	25	3.8885795E-5	45.000004	33
CGAACCT	30	2.1637698E-6	45.000004	36
GTTCTAT	25	3.8885795E-5	45.000004	40
GCGACGA	25	3.8885795E-5	45.000004	21
CGTATAA	25	3.8885795E-5	45.000004	28
CTACTAC	30	2.1637698E-6	45.000004	35
GATCGAA	25	3.8885795E-5	45.000004	9
CGAAAAG	25	3.8885795E-5	45.000004	2
CGCGGAT	30	2.1637698E-6	45.000004	3
>>END_MODULE