FastQCFastQC Report
Sat 14 Jan 2017
SRR2935002.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2935002.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876288
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC67280.7677841075080339TruSeq Adapter, Index 18 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC63380.723278191644756RNA PCR Primer, Index 34 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGCT44780.5110192082968156RNA PCR Primer, Index 34 (96% over 26bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGC36380.41516031259129416No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCG34640.3953038270522933No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC32900.37544734151329245RNA PCR Primer, Index 34 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC28950.3303708369851008TruSeq Adapter, Index 18 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTGC23210.26486725825299445TruSeq Adapter, Index 3 (95% over 24bp)
ACCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG14080.16067776803973122TruSeq Adapter, Index 3 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG12790.14595658048495472RNA PCR Primer, Index 34 (95% over 24bp)
GGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG11520.13146362839614373RNA PCR Primer, Index 34 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCC11390.1299800978673678No Hit
GCCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG10380.11845420683610867TruSeq Adapter, Index 18 (95% over 22bp)
AGCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTCTG9810.11194949605609116RNA PCR Primer, Index 34 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCGTGGCAACTCGTATGCCGTCTTC9080.10361890154834939TruSeq Adapter, Index 3 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTTCG750.045.00000422
CTACTAT302.1649084E-645.00000433
CGTATAA302.1649084E-645.00000426
GCGAAGT302.1649084E-645.00000412
TTATCCG302.1649084E-645.00000412
ATCGTCC302.1649084E-645.00000431
GTTACTT302.1649084E-645.00000432
ACTAGCG302.1649084E-645.0000041
TGCGCGT302.1649084E-645.00000437
ACGTCGG600.045.0000042
CATCGGT302.1649084E-645.00000418
ATCGGTT302.1649084E-645.00000419
GCGCTTA302.1649084E-645.00000414
TACGTCG302.1649084E-645.0000041
CGAAGTA302.1649084E-645.00000413
TATCCGA302.1649084E-645.00000413
TCACGCG207.032368E-445.040
CTATGCG207.032368E-445.01
TTAGGCA253.890044E-545.015
TCGCATA207.032368E-445.01
Produced by FastQC (version 0.11.5)